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- 1. ANTIMICROBIAL ACTIVE RELEASE COATING IN BIOMATERIALS Shirley Luo, (Dustin Lee Williams, PhD) Department of Orthopedics University of Utah, Salt Lake City, UT. 84112 The success of an implanted device may be diminished by biofilm implant-related infection. These infections may affect between 10% and 50% of patients with an implanted device. Antimicrobial coatings are being investigated as potential therapies to treat and prevent biofilm- related infections. This study investigated the ability of a unique antimicrobial compound, CZ-86, to act as the active release agent in a device coating on a simulated orthopedic implant in order to kill and disperse methicillin-resistant Staphylococcus aureus (MRSA). Stainless steel plates were primed and coated with three layers of polydimethlysiloxane (PDMS) containing CZ-86. Biofilms were grown on the surface of PEEK membranes by inoculating the bacteria in BHI broth. To evaluate the efficacy of CZ-86, the coated stainless steel plates and the bacteria covered PEEK membrane were screwed onto a piece of bone. The assembled unit was then placed in a flow cell system to simulate in vivo conditions wherein liquid flow would constantly dilute an active release product. Subsequently, the parts were disassembled and each piece was placed into its own broth solution, which was later used for quantification of the remaining bacterial cells to assess the efficacy of CZ-86. Results indicated that CZ-86 was able to reduce biofilms of MRSA by greater than 6log10 units in 24 hours. Based on the results of this study, CZ-86 is a promising compound to translate to an in vivo animal model in order to determine if it has the ability to treat and prevent biofilm implant-related infections. These data may lead to the development of new- coated devices to ultimately improve patient care.