This document discusses CABI's experience creating an online course on working with microorganisms. The course aimed to provide flexible, affordable training on sanitary and phyto-sanitary issues related to food security. It covers establishing and managing culture collections, characterizing and using microorganisms, best practices for identification, isolation, storage and growth, preservation techniques, health and safety requirements, and compliance with international standards. The course was developed based on CABI's 25 years of laboratory and field training experience in 75 countries, along with input from collaborators who provided pedagogical and technical expertise in online materials development. It includes introductory screens, lessons on core concepts, and practice exercises.