Membrane Proteomics Methods and Protocols 1st Edition Henry Bigelow
- 1. Membrane Proteomics Methods and Protocols 1st Edition Henry Bigelow - Downloadable PDF 2025 https://ebookfinal.com/download/membrane-proteomics-methods-and- protocols-1st-edition-henry-bigelow/ Visit ebookfinal.com today to download the complete set of ebooks or textbooks
- 2. Here are some recommended products that we believe you will be interested in. You can click the link to download. Proteomics Methods and Protocols 1st Edition Friedrich Lottspeich (Auth.) https://ebookfinal.com/download/proteomics-methods-and-protocols-1st- edition-friedrich-lottspeich-auth/ Membrane Transporters Methods and Protocols 1st Edition. Edition Qing Yan https://ebookfinal.com/download/membrane-transporters-methods-and- protocols-1st-edition-edition-qing-yan/ Membrane Transporters Methods and Protocols 1st Edition Qing Yan (Auth.) https://ebookfinal.com/download/membrane-transporters-methods-and- protocols-1st-edition-qing-yan-auth/ Clinical Proteomics Methods and Protocols 2nd Edition Antonia Vlahou https://ebookfinal.com/download/clinical-proteomics-methods-and- protocols-2nd-edition-antonia-vlahou/
- 3. Renal and Urinary Proteomics Methods and Protocols 1st Edition Visith Thongboonkerd https://ebookfinal.com/download/renal-and-urinary-proteomics-methods- and-protocols-1st-edition-visith-thongboonkerd/ Cancer Genomics and Proteomics Methods and Protocols 1st Edition Narendra Wajapeyee (Eds.) https://ebookfinal.com/download/cancer-genomics-and-proteomics- methods-and-protocols-1st-edition-narendra-wajapeyee-eds/ Plant Proteomics Methods and Protocols 1st Edition Valérie Méchin https://ebookfinal.com/download/plant-proteomics-methods-and- protocols-1st-edition-valerie-mechin/ Electron Crystallography of Soluble and Membrane Proteins Methods and Protocols 1st Edition Werner Kühlbrandt (Auth.) https://ebookfinal.com/download/electron-crystallography-of-soluble- and-membrane-proteins-methods-and-protocols-1st-edition-werner- kuhlbrandt-auth/ Quantitative Methods in Proteomics 1st Edition Katharina Podwojski https://ebookfinal.com/download/quantitative-methods-in- proteomics-1st-edition-katharina-podwojski/
- 5. Membrane Proteomics Methods and Protocols 1st Edition Henry Bigelow Digital Instant Download Author(s): Henry Bigelow, Burkhard Rost (auth.), MatthewJ. Peirce, Robin Wait (eds.) ISBN(s): 9781603273091, 1603273093 Edition: 1 File Details: PDF, 10.65 MB Year: 2009 Language: english
- 8. M E T H O D S I N M O L E C U L A R B I O L O G Y TM John M. Walker, SERIES EDITOR 528. Membrane Proteomics: Methods and Protocols,, edited by Matthew J. Peirce and Robin Wait 2009 510. Hepatitis C: Methods and Protocols, Second Edi- tion, edited by Hengli Tang 2009 507. DNA Methylation: Methods and Protocols, Second Edition, edited by Jörg Tost 2009 502. Bacteriophages: Methods and Protocols, Volume 2: Molecular and Applied Aspects, edited by Martha R. J. Clokie and Andrew M. Kropinski 2009 501. Bacteriophages: Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions, edited by Martha R. J. Clokie and Andrew M. Kropinski 2009 496. DNA and RNA Profiling in Human Blood: Methods and Protocols, edited by Peter Bugert, 2009 493. Auditory and Vestibular Research: Methods and Protocols, edited by Bernd Sokolowski, 2009 489. Dynamic Brain Imaging: Methods and Protocols, edited by Fahmeed Hyder, 2009 485. HIV Protocols: Methods and Protocols, edited by Vinayaka R. Prasad and Ganjam V. Kalpana, 2009 484. Functional Proteomics: Methods and Protocols, edited by Julie D. Thompson, Christine Schaeffer- Reiss, and Marius Ueffing, 2008 483. Recombinant Proteins From Plants: Methods and Protocols, edited by Loı̈c Faye and Veronique Gomord, 2008 482. Stem Cells in Regenerative Medicine: Methods and Protocols, edited by Julie Audet and William L. Stanford, 2008 481. Hepatocyte Transplantation: Methods and Proto- cols, edited by Anil Dhawan and Robin D. Hughes, 2008 480. Macromolecular Drug Delivery: Methods and Pro- tocols, edited by Mattias Belting, 2008 479. Plant Signal Transduction: Methods and Protocols, edited by Thomas Pfannschmidt, 2008 478. Transgenic Wheat, Barley and Oats: Production and Characterization Protocols, edited by Huw D. Jones and Peter R. Shewry, 2008 477. Advanced Protocols in Oxidative Stress I, edited by Donald Armstrong, 2008 476. Redox-Mediated Signal Transduction: Methods and Protocols, edited by John T. Hancock, 2008 475. Cell Fusion: Overviews and Methods, edited by Elizabeth H. Chen, 2008 474. Nanostructure Design: Methods and Protocols, edited by Ehud Gazit and Ruth Nussinov, 2008 473. Clinical Epidemiology: Practice and Methods, edited by Patrick Parfrey and Brendon Barrett, 2008 472. Cancer Epidemiology, Volume 2: Modifiable Fac- tors, edited by Mukesh Verma, 2008 471. Cancer Epidemiology, Volume 1: Host Susceptibil- ity Factors, edited by Mukesh Verma, 2008 470. Host-Pathogen Interactions: Methods and Proto- cols, edited by Steffen Rupp and Kai Sohn, 2008 469. Wnt Signaling, Volume 2: Pathway Models, edited by Elizabeth Vincan, 2008 468. Wnt Signaling, Volume 1: Pathway Methods and Mammalian Models, edited by Elizabeth Vincan, 2008 467. Angiogenesis Protocols: Second Edition, edited by Stewart Martin and Cliff Murray, 2008 466. Kidney Research: Experimental Protocols, edited by Tim D. Hewitson and Gavin J. Becker, 2008 465. Mycobacteria, Second Edition, edited by Tanya Parish and Amanda Claire Brown, 2008 464. The Nucleus, Volume 2: Physical Properties and Imaging Methods, edited by Ronald Hancock, 2008 463. The Nucleus, Volume 1: Nuclei and Subnu- clear Components, edited by Ronald Hancock, 2008 462. Lipid Signaling Protocols, edited by Banafshe Larijani, Rudiger Woscholski, and Colin A. Rosser, 2008 461. Molecular Embryology: Methods and Protocols, Second Edition, edited by Paul Sharpe and Ivor Mason, 2008 460. Essential Concepts in Toxicogenomics, edited by Donna L. Mendrick and William B. Mattes, 2008 459. Prion Protein Protocols, edited by Andrew F. Hill, 2008 458. Artificial Neural Networks: Methods and Applica- tions, edited by David S. Livingstone, 2008 457. Membrane Trafficking, edited by Ales Vancura, 2008 456. Adipose Tissue Protocols, Second Edition, edited by Kaiping Yang, 2008 455. Osteoporosis, edited by Jennifer J. Westendorf, 2008 454. SARS- and Other Coronaviruses: Laboratory Pro- tocols, edited by Dave Cavanagh, 2008 453. Bioinformatics, Volume 2: Structure, Function, and Applications, edited by Jonathan M. Keith, 2008 452. Bioinformatics, Volume 1: Data, Sequence Anal- ysis, and Evolution, edited by Jonathan M. Keith, 2008 451. Plant Virology Protocols: From Viral Sequence to Protein Function, edited by Gary Foster, Elis- abeth Johansen, Yiguo Hong, and Peter Nagy, 2008
- 9. M E T H O D S I N M O L E C U L A R B I O L O G Y TM Membrane Proteomics MethodsandProtocols Edited by Matthew J. Peirce, PhD and Robin Wait, PhD Kennedy InstituteofRheumatology Division, Faculty ofMedicine,ImperialCollegeLondon,UK
- 10. Editor Matthew J. Peirce Robin Wait Kennedy Institute of Rheumatology Division Kennedy Institute of Rheumatology Division Faculty of Medicine Faculty of Medicine Imperial College London Imperial College London UK UK m.peirce@imperial.ac.uk r.wait@imperial.ac.uk Series Editor John M. Walker University of Hertfordshire Hatfield, Herts UK ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60327-309-1 e-ISBN 978-1-60327-310-7 DOI 10.1007/978-1-60327-310-7 Library of Congress Control Number: 2008939875 c Humana Press, a part of Springer Science+Business Media, LLC 2009 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper springer.com
- 11. Preface The membranes surrounding cells and organelles constitute their interface with the local environment. The functions of membrane proteins include cell/cell and cell/extracellular matrix recognition, the reception and transduction of extracellular signals, and the trans- port of proteins, solutes and water molecules. Abnormal membrane protein expression has profound biological effects and may, for example, underlie phenotypic and functional differences between normal and tumour cells. Moreover the accessibility, particularly of plasma proteins traversing the plasma membrane of cells, makes them of particular util- ity to the therapeutic intervention in disease. Indeed, it is estimated that of all currently licensed pharmaceuticals, approximately 70% target proteins resident in the plasma mem- brane. In theory, unbiased technologies such as proteomics have the power to define patterns of membrane protein expression characteristic of distinct states of cellular development, differentiation or disease, and thereby identify novel markers of, or targets for intervention in, disease. However, although about 25% of open reading frames in fully sequenced genomes are estimated to encode integral membrane proteins, global analysis of membrane protein expression has proved problematic. Membrane protein analysis poses unique challenges at the level of extraction, solubilization, and separation in particular, and to a lesser extent of identification and quantitation. These challenges have, however, fostered creativity, inno- vation, and technical advances, many of which are brought together in Membrane Pro- teomics. Two-dimensional gel electrophoresis (2DE) can resolve mixtures containing many hundreds of components but membrane proteins are often severely underrepresented in 2DE protein patterns because of factors including their size, low abundance, and hydrophobicity. Thus, several chapters describe modifications to standard 2DE protocols; novel combinations of chaotropes, and detergents or solution-phase isoelectric focussing that improve recovery of membrane proteins. Other chapters discuss other electrophoretic methods such as blue-native gel, free-flow, and 16-BAC electrophoresis, which lend them- selves to the resolution of membrane proteins yet are not widely used. Several protocols focus specifically on the problems associated with the low abundance of membrane proteins; an array of protocols for the specific enrichment of plasma mem- brane proteins using organic solvents or detergents, chemical labelling of lysine or cysteine residues or proteolytic cleavage of PM proteins is presented. The selective enrichment of proteins derived from membranes of the nucleus, mitochondria, and lipid rafts are also covered. The limitations of 2DE have spurred the development of alternative, nongel based approaches to the resolution of membrane proteins. One approach is to generate a pro- teolytic digest of a membrane protein sample, the component peptides of which are then resolved by multidimensional chromatography prior to MS/MS. By analysing peptides derived from membrane proteins, rather than the proteins themselves, many of the prob- lems associated with hydrophobicity and large size of membrane proteins are avoided. v
- 12. vi Preface Such approaches have proved extremely useful for the study of membrane proteins not least because they lend themselves to techniques such as ICAT and iTRAQ, which enable the relative amounts of particular peptides in similar samples (e.g., resting versus activated cells) to be compared. Several chapters address both the application of such peptide “shot- gun” approaches to various types of membrane protein and their combination with ICAT and iTRAQ experiments to enable peptide quantitation. Another way to obviate the technical obstacles to proteomic examination of mem- brane proteins is to use an in silico approach. For sequenced genomes such efforts can be extremely informative and we have included several chapters describing how publicly avail- able bioinformatic tools can be used to predict membrane proteins and can be combined with other unbiased approaches, such as transcriptomic analysis, to acquire information regarding the membrane protein complement of a particular cell at a particular time. In practice, a strategy can be devised for the analysis of virtually any individual mem- brane protein; the challenge is to find methods capable of simultaneous analysis of large and diverse populations of such proteins. At the moment there seems to be no single tech- nology platform which enables global membrane proteome analysis, and it is necessary to apply methods in combination. This need for methodological pluralism is reflected in the diversity of procedures included in Membrane Proteomics. It is intended as a laboratory bench resource which provides a comprehensive toolkit of proven methods, contributed by leading experts in the field, for investigators wishing to apply state of the art membrane proteomic method- ologies in their own research programs. Mathew J. Peirce, PhD Robin Wait, PhD
- 13. Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi PART I. In Silico METHODS FOR PREDICTION OF MEMBRANE PROTEIN HYDROPHOBICITY AND TOPOLOGY 1 Online Tools for Predicting Integral Membrane Proteins Henry Bigelow and Burkhard Rost . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 2 In Silico Identification of Novel G Protein Coupled Receptors Matthew N. Davies and Darren R. Flower . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 3 Transcriptome-Based Identification of Candidate Membrane Proteins Edward J. Evans, Lawrence Hene, Mai Vuong, S. Hussain I. Abidi, and Simon J. Davis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 PART II. EXTRACTION AND PURIFICATION OF MEMBRANE PROTEINS PART A. PLANT MEMBRANE PROTEINS 4 Separation of Thylakoid Membrane Proteins by Sucrose Gradient Ultracentrifugation or Blue Native-SDS-PAGE Two-Dimensional Electrophoresis Gian Maria D’Amici, Christian G. Huber, and Lello Zolla. . . . . . . . . . . . . . . . . . . . . 61 PART B. PROKARYOTIC MEMBRANE PROTEINS 5 Extraction of Yeast Mitochondrial Membrane Proteins by Solubilization and Detergent/Polymer Aqueous Two-Phase Partitioning Henrik Everberg, Niklas Gustavsson, and Folke Tjerneld. . . . . . . . . . . . . . . . . . . . . . . . 73 6 16-BAC/SDS-PAGE Analysis of Membrane Proteins of Yeast Mitochondria Purified by Free Flow Electrophoresis Ralf J. Braun, Norbert Kinkl, Hans Zischka, and Marius Ueffing. . . . . . . . . . . . . . . 83 PART C. MAMMALIAN MEMBRANE PROTEINS 7 Sequential Detergent Extraction Prior to Mass Spectrometry Analysis Fiona M. McCarthy, Amanda M. Cooksey, and Shane C. Burgess. . . . . . . . . . . . . . . . . 111 8 Enrichment of Brain Plasma Membranes by Affinity Two-Phase Partitioning Jens Schindler and Hans Gerd Nothwang . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119 vii
- 14. viii Contents 9 Protocol to Enrich and Analyze Plasma Membrane Proteins Jacek R. Wiśniewski . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127 10 Proteomic Analysis of the Lymphocyte Plasma Membrane Using Cell Surface Biotinylation and Solution-Phase Isoelectric Focusing Matthew J. Peirce, Andrew P. Cope, and Robin Wait. . . . . . . . . . . . . . . . . . . . . . . . . . . . 135 11 Identification of Target Membrane Proteins as Detected by Phage Antibodies Cecile A.W. Geuijen, Arjen Q. Bakker, and John de Kruif. . . . . . . . . . . . . . . . . . . . . . . 141 12 Membrane Protease Degradomics: Proteomic Identification and Quantification of Cell Surface Protease Substrates Georgina S. Butler, Richard A. Dean, Derek Smith, and Christopher M. Overall . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 159 13 Purification of Basolateral Integral Membrane Proteins by Cationic Colloidal Silica-Based Apical Membrane Subtraction Robert J.A. Goode and Richard J. Simpson. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 177 14 Moving Closer to the Lipid Raft Proteome Using Quantitative Proteomics Leonard J. Foster. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 189 15 Use of Sequential Chemical Extractions to Purify Nuclear Membrane Proteins for Proteomics Identification Nadia Korfali, Elizabeth A. L. Fairley, Selene K. Swanson, Laurence Florens, and Eric C. Schirmer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 201 16 Isolation of Extracellular Membranous Vesicles for Proteomic Analysis Rommel A. Mathias, Justin W. Lim, Hong Ji, and Richard J. Simpson. . . . . . . . . . . 227 PART III. SEPARATION OF MEMBRANE PROTEINS 17 Enrichment of Human Platelet Membranes for Proteomic Analysis David W. Greening, Kristen M. Glenister, Rosemary L. Sparrow, and Richard J. Simpson. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 245 18 Detergents and Chaotropes for Protein Solubilization Before Two-Dimensional Electrophoresis Thierry Rabilloud . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259 19 Two-Dimensional Separation of Membrane Proteins by 16-BAC-SDS-PAGE Hans Gerd Nothwang and Jens Schindler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 269 PART IV. IDENTIFICATION AND QUANTIFICATION OF MEMBRANE PROTEINS 20 MudPIT Analysis: Application to Human Heart Tissue Kelli G. Kline and Christine C. Wu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 281
- 15. Contents ix 21 Liquid Chromatography MALDI MS/MS for Membrane Proteome Analysis Nan Wang, J. Bryce Young and Liang Li . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 295 22 Cysteinyl-Tagging of Integral Membrane Proteins for Proteomic Analysis Using Liquid Chromatography-Tandem Mass Spectrometry Srijeet K. Mitra and Michael B. Goshe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 311 23 Quantitative Proteomics Analysis of Pancreatic Zymogen Granule Membrane Proteins Xuequn Chen and Philip C. Andrews . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 327 Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 339
- 16. Contributors PHILIP C. ANDREWS • Department of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA ARJEN Q. BAKKER • Aimm Therapeutics, Amsterdam, Netherlands HENRY BIGELOW • CUBIC, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA RALF J. BRAUN • GSF-National Research Centre for Environment and Health, Institute of Human Genetics, Munich, Germany SHANE C. BURGESS • Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, MS 39762, USA GEORGINA S. BUTLER • Centre for Blood Research, University of British Columbia, Vancouver, BC, Canada XUEQUN CHEN • Department of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA ANDREW P. COPE • Kennedy institute of Rheumatology Division, Faculty of Medicine, Imperial College London, London, UK AMANDA M. COOKSEY • Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, MS 39762, USA GIAN MARIA D’AMICI • Department of Environmental Sciences, University of Tuscia, Viterbo, Italy MATTHEW N. DAVIES • Department of Crystallography, Birkbeck College, London, UK SIMON J. DAVIS • Nuffield Department of Clinical Medicine and MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK RICHARD A. DEAN • Centre for Blood Research, University of British Columbia, Vancouver, BC, Canada EDWARD J. EVANS • Nuffield Department of Clinical Medicine and MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK HENRIK EVERBERG • Department of Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Luund, Sweeden ELIZABETH A.L. FAIRLEY • The Wellcome Trust Centre for Cell Biology and Institute of Cell Biology, University of Edinburgh, Edinburgh, UK DARREN R. FLOWER • Edward Jenner Institute, Newbury, UK LAURENCE FLORENS • The Stowers Institute for Medical Research, Kansas City, MO, USA LEONARD J. FOSTER • UBC Centre for Proteomics, University of British Columbia, Vancouver, BC, Canada CECILE A.W. GEUIJEN • Genmab BV, Utrecht, Netherlands MICHAEL B. GOSHE • North Carolina State University, Department of Molecular and Structural Biochemistry, Raleigh, NC, USA xi
- 17. xii Contributors KRISTEN M. GLENISTER • Department of Biochemistry and Molecular Biology, University of Melbourne, Melbourne, Australia ROBERT J.A. GOODE • Joint Proteomics Laboratory, Ludwig Institute for Cancer Research The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia DAVID W. GREENING • Joint Proteomics Laboratory, Ludwig Institute for Cancer Research The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia NIKLAS GUSTAVSSON • Department of Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden LAWRENCE HENE • Nuffield Department of Clinical Medicine and MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK S. Hussain I Abidi • Nuffield Department of Clinical Medicine and MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK CHRISTIAN G. HUBER • Department of Environmental Sciences, University of Tuscia, Viterbo, Italy HONG JI • Joint Proteomics Laboratory, Ludwig Institute for Cancer Research The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia NORBERT KINKL • Technical University Munich, Institute of Human Genetics, Munich, Germany KELLI G. KLINE • Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO, USA NADIA KORFALI • The Wellcome Trust Centre for Cell Biology and Institute of Cell Biology, University of Edinburgh, Edinburgh, UK JOHN DE KRUIF • Merus BV, Utrecht, Netherlands LIANG LI • Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada JUSTIN W. LIM • Department of Biochemistry and Molecular Biology, University of Melbourne, Melbourne, Australia ROMMEL A. MATHIAS • Joint Proteomics Laboratory, Ludwig Institute for Cancer Research The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia FIONA M. MCCARTHY • Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, MS 39762, USA SRIJEET K. MITRA • North Carolina State University, Department of Molecular and Structural Biochemistry, Raleigh, NC, USA HANS GERD NOTHWANG • Abteilung Neurogenetik, Institut für Biologie und Umweltwissenschaften, Oldenburg, Germany CHRISTOPHER M. OVERALL • Centre for Blood Research, University of British Columbia, Vancouver, BC, Canada MATTHEW J. PEIRCE • Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College London, London, UK THIERRY RABILLOUD • Laboratoire, d’immunologie, DRDC/ICH, INSERM, Grenoble, France BURKHARD ROST • Columbia University Center for Computational Biology and Bioinformatics, Irvine Center for Cancer Research, New York, NY, USA
- 18. Contributors xiii JENS SCHINDLER • Abteilung Neurogenetik, Institut für Biologie und Umweltwissenschaften, Oldenburg, Germany ERIC C. SCHIRMER • The Wellcome Trust Centre for Cell Biology and Institute of Cell Biology, University of Edinburgh, Edinburgh, UK RICHARD J. SIMPSON. Joint ProteomicS Laboratory, Ludwig Institute for Cancer Research The Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Parkville, VIC, Australia DEREK SMITH • University of Victoria Proteomics Centre, Vancouver Island Technology Park, Victoria, BC, Canada ROSEMARY L. SPARROW • Research Unit, Australian Red Cross Blood Service (ARCBS), Melbourne, Australia SELENE K. SWANSON • The Stowers Institute for Medical Research, Kansas City, MO, USA FOLKE TJERNELD • Department of Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden MARIUS UEFFING • GSF-National Research Center for Environment and Health, Institute of Human Genetics and Technical University Munich, Institute of Human Genetics, Munich, Germany MAI VUONG • Nuffield Department of Clinical Medicine and MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK ROBIN WAIT • Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College London, London, UK NAN WANG • Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada JACEK R. WIŚNIEWSKI • Department of Proteomics and Signal Transduction, Max-Planck Institute for Biochemistry, Martinsried, Germany CHRISTINE C. WU • Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO, USA BRYCE YOUNG • Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada HANS ZISCHKA • GSF-National Research Center for Environment and Health, Institute of Toxicology, Munich, Germany LELLO ZOLLA • Department of Environmental Sciences, University of Tuscia, Viterbo, Italy
- 19. Part I In Silico Methods for Prediction of Membrane Protein Hydrophobicity and Topology
- 20. Chapter 1 Online Tools for Predicting Integral Membrane Proteins Henry Bigelow and Burkhard Rost Abstract We identify and describe a set of tools readily available for integral membrane protein prediction. These tools address two problems: finding potential transmembrane proteins in a pool of new sequences, and identifying their transmembrane regions. All methods involve comparing the query protein against one or more target models. In the simplest of these, the target “model” is another protein sequence, while the more elaborate methods group together the entire set of transmembrane helical or transmembrane beta-barrel proteins. In general, prediction accuracy either in identifying new integral membrane proteins or transmembrane regions of known integral membrane proteins depends strongly on how closely the query fits the model. Because of this, the best approach is an opportunistic one: submit the protein of interest to all methods and choose the results with the highest confidence scores. Key words: Membrane protein structure prediction, transmembrane helix, transmembrane beta barrel, hidden Markov model, neural network, remote homolog detection, proteome searching. 1. Introduction Experimentalists often work with proteins of unknown or par- tially known structure or function. For transmembrane proteins, there are two types of predictions that can be made. The more common type is identifying transmembrane regions and orienta- tion of a protein already known or suspected to be integral to the membrane. Less common is the problem of identifying all potential integral membrane proteins in a pool of proteins. A vari- ety of online services to solve both problems are available, but it is often time-consuming to find them and especially to discern which methods are reliable and how to interpret the results. Here we provide a guide to aid experimentalists in this endeavor. Matthew J. Peirce, Robin Wait (eds.), Membrane Proteomics: Methods and Protocols, vol. 528 C Humana Press, a part of Springer Science+Business Media, LLC 2009 DOI 10.1007/978-1-60327-310-7 1 Springerprotocols.com 3
- 21. 4 Bigelow and Rost 1.1. Basic Concept of Alignments At a basic level, all methods work by the same paradigm. The simplest of these is BLAST. BLAST aligns the query sequence with each target sequence in a database. The alignment algorithm assigns a score to each alignment of query and target using a 20 × 20 matrix of scores called a “substitution matrix.” The sub- stitution matrix quantifies how often proteins whose sequences are aligned, based on known structure, have the same or differ- ent amino acids at each position. The alignment score involves summing substitution matrix values along with scores associated with gaps. Finally, taking all these alignments, a score threshold identifies a subset as target homologs. 1.2. Homology-Transfer through Alignments Available experimental information for any of the targets can be transferred to the query (homology-transfer). For example, if one of the targets (database proteins) is experimentally known to be a transmembrane helical (TMH) protein, the homologous query is likely to also be a TMH protein. Moreover, if particular regions of a target protein are known to be TMHs, the regions in the query aligned to these regions are also likely to be TMHs. Of course, both inferences are subject to the accuracy of the alignment and the similarity between the two proteins. As with all elements of living things, protein sequences orig- inate from an evolutionary process of divergence and selection, creating a tree of proteins related in hierarchical fashion. Extend- ing this idea to the homology search, a query protein can be compared to an entire family of related target proteins that are prealigned. Often, where a query might not have apparent simi- larity to any individual target protein in a family, it may have simi- larity to the target family taken as a whole. Essentially all advanced methods implement this idea. 1.3. Improved Profile-Based Alignment Methods A well-known example of this extension is PSI-BLAST (1), which works as follows. First, the query is searched against a database of individual sequences using ordinary BLAST, resulting in a set of query-target alignments. Next, the query and set of target proteins are aligned to each other in a single multiple-sequence alignment. The frequencies of each amino acid as occurring in the columns of the multiple-sequence alignment are calculated, resulting in a set of 20-element vectors, one for each posi- tion in the original query. This statistical representation, called a position-specific score matrix (PSSM) can be seen as a substi- tution matrix, custom designed for each position in the query protein. In subsequent rounds, the PSSM, rather than the orig- inal query, is searched against the original database of individual sequences. For statistical reasons, conserved regions tend to be more influential in scoring subsequent alignments, allowing for improved detection of more diverged sequences.
- 22. Online Tools for Predicting Integral Membrane Proteins 5 Similar to PSI-BLAST, Pfam (2) uses multiple-sequence alignments. There are two differences, however. First, while PSI- BLAST iteratively re-queries a database of individual sequences with a PSSM, Pfam is the inverse: it is a database of protein fami- lies, and the individual query protein is aligned against each family in the database. Second, while PSI-BLAST uses PSSMs to repre- sent a protein family multiple sequence alignment, Pfam uses a hidden Markov model (HMM). An HMM extends the idea of position specific substitution scores to include gap insertion and deletion scores that are also position specific. These are possi- ble to derive from the original multiple-sequence alignment by observing how many aligned proteins contain insertions or dele- tions relative to the query protein at each position in the query. As in PSI-BLAST or BLAST, the query protein is aligned against each HMM in the Pfam database and assigned an E-value compa- rable to BLAST E-value, representing the expected number of matches as good or better occurring by chance. Since HMM- based alignment methods are often more sensitive than BLAST or PSI-BLAST, they may succeed in finding a homologous family. BLAST, PSI-BLAST, and Pfam are very general methods capable of identifying sequence or family homologs of virtually any kind of protein, including specific kinds of membrane pro- teins. For integral membrane protein prediction however, another generalization yields further improvement. 1.4. Two Major Classes of Transmembrane Proteins: TMB and TMH Integral membrane proteins come in two general structural classes. Transmembrane alpha helical (TMH) proteins span the plasma membrane in one or more alpha helices in alternating direction (Fig. 1.2). TMH proteins can be found in plasma membranes, organelle membranes, archaea, and bacteria, except the outer membranes of Gram-negative bacteria. Transmembrane beta-barrel (TMB) proteins reside exclusively in outer membranes of Gram-negative bacteria, atypical Gram-positive (“mycolata”) bacteria, mitochondria, and chloroplasts (Fig. 1.1). TMBs form an “all-next-neighbor” beta-barrel in the membrane, meaning each transmembrane strand neighboring in sequence is physically adjacent in the beta-sheet forming the barrel. N- and C-termini always reside in the periplasm, dictating an even number of trans- membrane strands. Examples range from 8 (OmpX) to 22 (FepA) transmembrane strands. 1.4.1. Specific Prediction Methods Methods designed to predict TMH or TMB proteins in general are built on each class taken as a group. Because of the diversity in specific structure (different numbers of transmembrane helices or strands), it is impossible to derive a single multiple sequence alignment for such a class. Instead, these methods extract fea- tures in common to all TMH or all TMB proteins without the need for explicit multiple-sequence alignment. Technically, this is
- 23. 6 Bigelow and Rost Fig. 1.1. TMB per-residue prediction methods. Per-residue predictions from the three best-performing TMB methods are displayed for long-chain fatty acid transport protein from E. coli, both linearly and mapped onto the 3D structure (PDB: 1T16 (10)). PDBTM and OPM, both 3D structure-based estimates of relative membrane orientation, are used for comparison against per-residue predictions. Note that the agreement between the methods for this example is not representative. A. 3D structure illustration. Inner loops, TM-β-strands, and outer loops are depicted. Note that none of the programs actually predicts such a 3D ribbon plot, instead the actual predictions are as shown in B. B. Linear display. Inner loop, TM-β-strands, and outer loop are depicted respectively by a thin line, thick line, and no lone. 3D structure images rendered by GRASP (47). achieved by assigning one of a set of discrete labels to each posi- tion in each sequence, based on its structure. For example, the set of labels T, I, and O can be assigned one per residue to each TMH sequence, identifying the transmembrane helices, inner and outer loops. From the resulting set of labeled protein sequences, a general model (also often a HMM) can be derived that recog- nizes features common to all labeled protein sequences. Such gen- eral models are potentially able to detect TMH or TMB proteins even further diverged from any sequence homolog, (perhaps an example of a previously undiscovered subfamily), than sequence- alignment based methods such as PSI-BLAST and Pfam. A different homology approach is exemplified in the PROSITE (3) and PRINTS (4) databases. They contain a set of local sequence patterns defined by strong association with a specific protein function or structure. Because protein function and structure can be modular, some of these patterns may be found within a collection of proteins differing in overall struc- ture. Others are very well correlated with overall structure despite their sequence-local nature. For identifying TMH or TMB pro- teins, several patterns prove useful (see Methods Section). Poten- tially, such patterns may be conserved in a protein whose overall sequence is so diverged from any homologs as to be unidentifiable by alignment-based methods. In general, all methods relying on alignment of proteins work optimally in aligning proteins in a specific similarity range
- 24. Online Tools for Predicting Integral Membrane Proteins 7 corresponding to the range of sequences from which they are derived. In a degenerate sense, BLAST can be thought of as searching a database of “models” consisting of individual sequences. It is optimized to find close-range homologs. PSI- BLAST and Pfam build statistical models from multiple align- ments of very similar sequences, and they work best to find medium-range homologs. TMH and TMB-specific methods are single statistical models built from a diverse set of TMH or TMB proteins only related by broad structural category. Thus, they are optimized to find long-range homologs. Optimal results of each of these methods will be obtained fortuitously when the query happens to have a single sequence homolog, homology to a sequence family, or homology to a struc- ture family. It is impossible to know in advance which if any of these will be the case. Because of this, we recommend an oppor- tunistic approach: run all prediction methods and select those giv- ing highest confidence scores. We provide a guide for obtaining as much relevant information about your protein as possible, and some general principles for interpreting the information. This guide is in three parts. Firstly, we describe how to obtain a quick, comprehensive set of homology-based information and possible experimental information about your protein, and how to use it to identify whether it is an integral membrane pro- tein. Secondly, we describe those methods suitable for screening an entire set of proteins for potential TMH or TMB proteins. Thirdly, we present the methods for predicting which residues in a known or suspected transmembrane protein are in the mem- brane, and the overall orientation in the membrane. For quick reference, we provide a list of selected programs (Table 1.1) and databases (Table 1.2). 2. Methods 2.1. Determining If Your Protein Is Integral to the Membrane There are TMH- or TMB-specific and general methods available. The general methods are motif- and domain-based, and poten- tially identify the protein as one of a subtype of TMH or TMB proteins. TMH- or TMB-specific methods are designed to iden- tify features common to all TMH (or all TMB) proteins, and do not identify subtypes. InterProScan is a portal that allows query- ing the general methods at once. UniProt provides a compre- hensive view of previously analyzed results on many proteins and accompanying experimental information on structure or function. 2.1.1. TMB-Specific Methods BOMP (β-barrel outer membrane protein predictor), TMB- HUNT, and PROFtmb are specially designed to identify TMB proteins in a pool. They have all been evaluated for accuracy in
- 25. 8 Bigelow and Rost Table 1.1 Selected programs Method Scope Service URL References BLAST and PSI-BLAST general WP www.ncbi.nlm.nih.gov/BLAST (1,15) TMHMM TMH PR3, S www.cbs.dtu.dk/services/TMHMM (16) PiMohtm TMH PR3 www.predictprotein.org (17) Phobius TMH PR3, SP, S phobius.cgb.ki.se (18) HMMTOP TMH PR3 www.enzim.hu/hmmtop (19) MEMSAT TMH PR5 bioinf.cs.ucl.ac.uk/psipred (20) Split4 TMH PR2 split.pmfst.hr/split/4 (21) PRED-TMBB TMB PR3 bioinformatics2.biol.uoa.gr/PRED-TMBB (22,23) HMM-B2TMR TMB PR3 gpcr.biocomp.unibo.it (24) PROFtmb TMB PR3, S rostlab.org/services/proftmb (25) TMB-HUNT TMB S www.bioinformatics.leeds.ac.uk (26,27) BOMP TMB S www.bioinfo.no/tools/bomp (28) SignalP SP SP, S www.cbs.dtu.dk/services/SignalP (29) Pfam domain WP www.sanger.ac.uk/Software/Pfam (2) Superfamily domain WP supfam.mrc-lmb.cam.ac.uk/SUPERFAMILY (30) Panther domain WP www.pantherdb.org (31) SMART domain WP smart.embl-heidelberg.de (32,33) PROSITE motif WP ca.expasy.org/prosite (3) PRINTS motif WP umber.sbs.man.ac.uk/dbbrowser/PRINTS (4) Selected programs and databases for identification and per-residue prediction of integral membrane proteins. Scope. TMH, TMB: built on a representative collection of TMH or TMB proteins. motif: built on short sequence motifs associated with particular function or structure. domain: built on medium to long sequence regions of partic- ular structure. Service. Per residue predictions PRn: all residues are assigned to one of a number of discrete structural states; PR2: TM, non-TM; PR3: TMB: TM-strand, extra cellular loop, and periplasmic loop. TMH: TM-helix, cyto- plasmic loop, and noncytoplasmic loop. PR5: PR3, but distinguishing non-TM portions of helical overhang on both sides. SP: Signal peptide and cleavage site prediction. S: suitable for whole-proteome screening; these methods all allow multiple-sequence submission and have been evaluated for accuracy and coverage in whole protein discrimina- tion. WP: whole protein prediction of individual proteins. discriminating TMBs from background. Unfortunately, a defini- tive comparison is complicated by the fact that the evaluations are all done on different data sets. It is recommended that you submit your query to all three and scrutinize the results. Taking a con- sensus of predictors has been found consistently to yield better accuracy than relying on one individual predictor.
- 26. Another Random Scribd Document with Unrelated Content
- 27. Sixth Generation. -7540, The following was received after this work had gone to press : •40S1. Judge James Phelps, b. Colebrook, 12 Jan., 1S22, m. Lydia A.Ingham, of Essex, Conn., daughter of Hon. Samuel and Lydia (Wilson) Ingham, b. Essex, 28 Nov., 1819. Mr. Ingham was a member, of the 24th and 25th Congresses with Dr. Lancelot Phelps, father of Judge Phelps, and held many other important offices under the State and National governments, and was an able and distinguished lawyer and citizen, Mr. Phelps entered Washington, now Trinity College, but on account of severe illness was soon obliged to relinquish his course. He was a member of the law department of Yale College, and admitted an attorney in 1844, and located in Essex where he has ever since resided. Besides holding the office of Judge of Probate and other local positions, he was a member of the House of Representatives, in the State Legislatures of 1853', 1854 and 1856, and of the State Senate in 1858 and 1859. In 1863 he was elected by the General Assembly a Judge of the Superior Court for the regular term of eight years. He was re-elected in 1871, and in iSj; was elected a Judge of the Supreme Court of Errors. Ht resigned in 1875 on his election to the 44th Congress from the Second Congressional District, comprising the counties of New Haven and Middlesex. He was re- elected to the 45th, 46th and 47th Congresses and declined further Congressional service. He was again elected a Judge of the Superior Court in January, 1885, which position he held until disqualified by age in 1S92. While in Congress he served on the Standing Committees of Ways and Means, Foreign Affairs, Reform in the Civil Service, and others, and on several important special committees, including that to investigate alleged frauds in the State of Louisiana in the Presidential election of 1876. He procured the establishment of the break-water at the entrance of New Haven harbor, and liberal appropriations for the improvement of the channel of the Connecticut river below Hartford and at its mouth, and other needed fmprovements in his district. He is now retired from active professional
- 28. J5j.b Sixth Generation. business, and is President of the Saybrook Bank, and the Essex Savings Bank, and is Senior Warden of Saint John's Episcopal Parish and Church, to which he is a liberal contributor, and of which he has for more than thirty years been a devoted member,
- 29. Seve?ith Generation. 75-5and later removed to Monroe Heights, Cortland, N. Y., where he died. His widow now resides at Homer, N. Y. •1087. Ralph Burnham Phelps, b, Norfolk, Ct., 27 April, 1S27, m. Sarah Jane Whitby, 11 June, 1849, in Eastport, Me. She was daughter of Thomas and Sarah (Garry) Whitby, and was b. Eastport, Me., 7 April, 1831, d. Portland, Me., 25 Dec, 1S64. Mr. Phelps settled in Eastport, Me., later removing to Ohio, and was at the National Military Home, Montgomery Co., Ohio, in 1897. Children : + 7834- I- William Harvey, b. Eastport, Me., 13 May, 1850, m, Elizabeth Lyshon, 7835. II. Maria Euphrazee, b. St. Lawrence Co., N. Y., 3 April, 1853, m. Skinner. Res. 582 Boston St., Lynn. 4091. Cicero Claremont Phelps, b. West Groton, N. Y., 11 Feb., 1S45, m, Sarah Thompson. Settled in Homer, N, Y. Children, b. Homer, N. Y.: 7836. I. Homer, b. 5 June, 1879. 7S37. II. Claude, b. 4 April, 1882. 7838. III. Leona, b. 29 Jan., 1890. 4092. Aitrelia Phelps, b. Colebrook, Ct., Dec, 3, 1802, d. Lincoln, Ind,, August 21, 1880, m. in Waterbury, Ct., Dec. 4, 1821, Alvah Hoadley, b. Waterbury, Ct., Feb. 19, 1800, d. Lincoln, Ind., June 26, 1879, son of Asa and Esther (Tyler) Hoadley. Mr. Hoadley was a farmer in Waterbury, Ct., until 1S38, when he removed to Hendricks County, Indiana, and in the following spring settled on a farm in the town of Lincoln. In Waterbury he was a justice of the peace, and in Lincoln a township trustee. Children, b. Waterbury, Ct.: 7S39- I- James Gilbert (Hoadley), b. Sept. 16, 1824, m. Elizabeth Larsh. 7840. II. Asa Thomas (Hoadley), b. Oct. 22, 1827, m. Rebecca E. Shirley.
- 30. 75 Seventh Generation. 4093. Roxy Matilda Grant, b. Norfolk, Ct.,. Sept. 2, 1795, d. Oct. 24, 1841, m. May 17, 1S18, GeorgeCook, b. Goshen, Ct., July 24, 1791, d. Feb, 10, 1864, son of Moses and Matilda (Thompson) Cook. Res. Goshen, Ct. Mr. Cook was a merchant. Children, b. Goshen, Ct,: 7841. I. Caroline M. (Cook), b. June 10, 1819, d. Feb. 10, 1876, unra. 7842. II. Ralph F. (Cook), b. May 5, 1822, d. Goshen, Sept, 26, 1884, m. June 18, 1872, Julia Eliza Wooster, b. Goshen, July 14, 1830, daughter ■ of Sterling and Phebe (Sherman) Wooster. No issue. Mrs. Cook res. Goshen 1897. 4094. Lois Fucinda Grant, b. Norfolk, Ct., 2 Sept., 1795, m. Benedict Sweet, May, 1819. He was sou of John and Phebe (Spencer) Sweet, and was b. Winchester, Ct., 15 Oct., 1796. Mr. Sweet first settled in Tyringham, Mass., from there to Wellington, Ohio, and from there to Fond Du Fac, Wis., where he d. 8 July, 1S48, and where his widow resided in 1865. Children : 7S43. I. Moses (Sweet), b. about 1821, resides in Oak Centre, Wis. 7844. II. Sumner Boyingtou (Sweet), b. Mentor, Ohio, 20 March, 1823, d. Byron, 27 Dec, 1896, m. Elizabeth Evans, at Waupun, Wis., 24 Oct., 1847. She was b. England, 29 July, 18 1 9. They reside in Byron. Had 2 sons and 3 daughters, 4096. Joel Marshall Grant, b. Norfolk, Ct., 3 March, 1799, m. Jeunette Greeley, at Covington, N. Y., 6 Sept., 1826. She was daughter of Samuel and Caroline (Phelps) Greeley, and was b. at Waterbury, Ct., 22 Aug., 1807. She returned to Waterbury, Ct., in 1S44, and died 29 Sept., 1896, Mr. Grant resided in Covington, N. Y., where he d. 10 July, 1843.
- 31. Seventh Generation. yjy Children, b. Covington, N. Y. : + 7S45. I. Caroline M. (Grant), b. March 26, 1830, m. Charles B. Merrill. 7546. II. Marietta Sophia (Grant), resides New Haven, Ct. 7547. III. Mar}* Ann Desire (Grant), resides 236 Hillside Ave., Waterbnry. 7S4S. IV. George Moses (Grant), resides Chautauqua, N. Y. 4097. Giles Phelps Grant, b. Norfolk, Ct., 26 May, 1S01, m. 1st Laura Chittenden, 1 Feb., 1825. She was daughter of Amos and Laura (Gould) Chittenden, and was b. Hartford, Ct., 15 April, 1862. He m. 2nd Margaret McLean, 28 Sept., 1S63. She was daughter of Hector and Anna (Mclntyre) McLean, and was b. Caledonia, N. Y. Mr. Grant in 1822, removed to Hartford, and opened a wholesale and retail store, which he managed with success for 29 years, removing in 1851 to Ithaca, N. Y., and in 1852, to Rochester, N. Y., where he was in business in 1865. No issue. They adopted a daughter who m. Charles Wilde, a cotton manufacturer, and resides in Kinderhook, N. Y. 4099. Harry McGill Grant, b. Norfolk, Ct., 8 Feb., 1806. m. Sarah Ann Richards, at Winchester, Ct., 1 Oct., 1834. She was daughter of Moses and Naomi (Hulburt) Richards and was b. Otis, Mass., 4 Feb., 1813. Mr. Grant settled in Norfolk, Ct., on the homestead of his father and grandfather. He d. 20 Sept., 1870. Children, b. Norfolk, Ct.: 7849. I. Moses Franklin (Grant), b. 26 Jan., 1835, m. Mary Ann Gilbert. Res. Norfolk, Ct. 7850. II. Lucy Ann (Grant), b. 2 Nov., 1836, m. Isaac Coe. Res. West Wiusted. 7851. III. Mary Jane (Grant), b. 1 July, 1839, m. Edward Manchester. 7852. IV. Roxauna Matilda (Grant), b. 1 Aug., 1841, m. Sylvanus E. Granger, 15 Nov., 187 1. Res. Winsted, Ct.
- 32. j 58 Seve?ith Generation. 7853. V. Sarah Elizabeth (Grant), b. 3 August, 1846, m. George Johnson. She d. 30 June, 1869. One son, 7854. VI. George Phelps (Grant), b. 30 Oct,, 1848, d, 23 Nov., 1874, unm. 7855. VII. Henry Richard (Grant), b. 26 July, 185 1. Res. Millerton, N. Y. 7856. VIII. Miles Eugene (Grant), b. 21 April, 1855. Res.. Torrington, Ct. 4103. Riley Andrews Grant, b. Norfolk, Ct., 15 July, 1817, m. Huldah Rebecca Tibbals, 20 May, 1S43. She was daughter of Nathan and Rebecca (Green) Tibbals, and was b. Winchester, Ct.. 6 Jan., 182 1, d. Norfolk, Ct., 27 Feb., 1893. Mr. Grant settled in Norfolk, Ct. Child, b. Norfolk, Ct.: 7857. I. Garry C. (Grant), b. 20 Oct., 1849, m. Ellen E. Phelps. See No. 4104. Oliver Phelps, b. Unadilla, N. Y., 10 June, 1809, in. Emily Fallett, at Hector, N. Y., 10 March, 1831. Mr. Phelps was a farmer. He first settled in Tompkins' Co., and from there to Schuyler Co., and finally at Cuba, N. Y., where he d. 5 April, 18S7. Children: 7858. I. Helen, b. Hector, N. Y., 24 May. 1832, m. Silas J. Eldrad, 20 March, 1853, at Bennettsburgh, N. Y. 7859. II. Nancy A., b. Covert, N. Y., 1 Sept., 1833, m. Harvey Brown, 16 Jan., 1854, at Bennettsburgh, N. Y. 7860. Ill, Eucy M., b. Covert, N. Y., 13 April, 1835, m. Euther Chandler, 3 July, 1852. 7861. IV. Augusta A., b. Haskell Flats, N. Y., 23 Oct., 1837, m, Henry Smith, 7 Oct., 1854, 7862. V. Cicero Horace, b. Haskell Flats, N. Y., 15 Dec, 1839, d. Cuba, N. Y,, S April, 1SS7,
- 33. Seventh Generation. ?jp unm. Was a soldier in war of the Rebellion, enlisted in the outbreak. + 7863. VI. Harlow William, b. Haskell Flats, N, Y., 26 May, 1842, m. Emma Kyle. + 7S64. VII. Warren O., b. Hector, N. Y., 1 Sept., 1845, m. Louisa Brown. 4105. Emily Phelps, b. Unadilla, N. Y., 181 1, m. Andrew Beach, 22 June, 1832. Settled in Cuba. N. Y., where he died. She 111. 2nd Webb. Settled in Cuba, N. Y., where he d. March, 1S82. Child b}^ 1st husband : 7865. I. Alvin (Beach), b. Cuba, N.Y., 8 Nov. Children by 2nd husband : 7866. II. (Webb), b. Cuba, N. Y. 7S67. III. b. 786S. IV. b. 4106. Cyrus Phelps, b. Lansing, N. Y., 26 July, 1813, m. Charlotte Howe, 20 Dec, 1840, at Haskell Flats, N. Y. She was daughter of Ebenezer and Sarah (Hampton) Howe, and was b. Haskell Flats, N. Y., 4 Feb., 1 82 1, d. at Carthage, Mo., 15 June, 1880. Mr. Phelps settled in Haskell Flats, N. Y., where he d. 4 Jnly, 1874. He was a farmer. Children, b. Haskell Flats, N. Y.: 7869. I. Clarissa, b. 1842, d. 1844. + 7870, II. William Harlow, b. 16 Oct., 1S45, m. Lois J. Wilson. + 7871. III. Cyrus Emmet,- b. 29 May, 1848, m. Agnes E. Keller. + 7S72. IV. Charles Hampton, b. 7 Dec, 1S56, m, Ina Burlingame. 7873. V. Lucy May, b. Sept., 1859, m. Charles Burton. Settled in Haskell Flats, N. Y. 4107. Augustus Harlow Phelps, b. Lansing, N. Y., 11 August, 1816, m. Olive Quin, at Milford, N. Y., 25 May, 1848. She was b. 17 Dec, 1820, daughter of Patrick and Betsey (Wolcott) Quin.
- 34. y6o Seventh Geyieration. Mr. Phelps is a farmer, first settled in Hector, N. Y., and from there in 1S54, to Haskell Flats, N. Y., where he now resides. Children : + 7874. I. Bessie, b. Hector, N, Y., 7 Jan., 1853,. m. George B. Rounsevell. + 7875. II. George H., b. Haskell. Flats, N. Y. 24 Sept., 1854, m. T. Adelle Canfield. 7876. III. George E., b. Haskell Flats, N. Y. 10 July, 1862, d. Sept., 1862. 4110. William Calvin Phelps, b. Colebrook, Ct., 4 Sept., 1807, m. Parney Cowles, 10 April, 1832. She was daughter of Moses and Hannah (Bates) Cowles, and was b. Norfolk, Ct., 7 Sept., 1S07, d. West Wiusted, Ct., 15 Feb., 1873. He m. 2nd Mrs. Harriet E. (Munson) Butler, in Chicago, 111., 23 Oct., 1873. She was b. Barkhamsted, Ct., 11 Feb., 1822, d. July 14, 1897, daughter of Sherlock Munson, and widow of William Butler. Mr. Phelps removed with his parents in 181 1 to Kingsviile, Ohio, where he resided up to 1827, when he returned to Ct. In 1832 he settled in Norfolk, Ct., on a farm of his father-inlaw, and was engaged in dairy and cheese business up to 1854, when he moved to Winsted, Ct., and was secretary of the savings bank and building association of that place. During the War of the Rebellion he was town agent to fill the quota and attend to the wants of the soldiers. Since 1874, and after marrying his 2nd wife, most of .his time has been spent in traveling in the states and territories. Res. Winsted, Ct. Is now living in good health (July, 1899). He has been many times selectman. Children, b. Norfolk, Ct.: + 7877. I. Dwight, b. 3 Sept., 1834, m. Diana Lowe. 7878. II. Mary P., b. 16 Dec, 1836, m. Jeuisou J. Whiting. Hed. 16 Dec, 1836, son of Julius and Duciuda (Payne) Whiting. No issue. + 7879. III. William B., b. 2 June, 1843, m. Elizabeth D. Osborn. 7880. IV. John Betts, b. 10 Feb., 1S4S, d. 24 Sept., 1848.
- 35. Seventh Generation. j6i Note. — Sou of Moses- Cowles, Ebenezer5, Samuel*, Samuel3, John3, John1 Cowles, res. Hartford, 1635 to 1639, Farming-ton 1640, Hadley 1659, Hartford 1660, where he d. 1676. 4111. Daniel Milton Phelps, b. Colebrook, Ct., 3 June, 1809, m. Celestia Perviua, 12 Dec., 1833. Mr. Phelps in 1S11 removed with his father to Kiugsville, Ohio, where he settled after marrying. In 1837 he removed to Sheffield, Ohio, where he died March, 1899. Children, b. Sheffield, Ohio, except first two: 7581. I. Pheonessa Merula, b. Kiugsville, Ohio, 6 July, 1835, m. Clark, of North Kiugsville. Ohio. 7582. II. Frances Ellen, b. Kiugsville, Ohio, 21 Oct., 1836, d. 31 Oct., 1836. 7883. III. Marcus Eugene, b. 4 April. 1838. 7S84. IV. Rosanna B., b. 8 May, 1S40. 78S5. V. Ina, b. 30 June, 1S42. 7886. VI. Phebe Eudora, b. 2 Dec, 1843. 7S87. VII. Sarah Angeline, b. 27 Dec, 1845, d. 7 July, iS5478SS. VIII. William Calvin, b. 20 Oct., 1847. 4116. Francis Barnard Phelps, b. Kiugsville, Ohio, 16 August, 18 18, in. Margaret Scott Saunders, 6 Oct., 1S37. She was daughter of Abram Saunders and wasb. Ashtabula, Ohio, Mr. Phelps settled in Kingsville, Ohio. Child: 7889. I. Sarah Jane, b. Kingsville, Ohio, 1839. 4118. Elizabeth Phelps, b. Colebrook, Ct., 6 March, 1817, m. Asaph O. Piuney, 17 May, 1840. He was son of Asaph and Betsey (Wilcox) Pinney, and was b. Colebrook, Ct., 9 Nov., 1805. Settled in Mill Brook, Colebrook, Ct. He d. 16 April, 1889. No issue. 4119. Martin Luther Phelps, b. Colebrook, Ct., 10 Jan., 1S20, m. 1st 27 Nov., 1S50, Charlotte N. Sage, b. Colebrook, Ct., 1821, where she d. 14 March, 1856. He m. 2nd 6 May, 1857, Susan J. Muuson, b. Sandisfield, Mass., 3 Feb., 1S35. Mr. Phelps settled in Colebrook, Ct. He d. 12 March, 1S87.
- 36. 762 Seventh Generation, Child by 1st wife, b. Colebrook, Ct.: + 7890. I. Luther C, b, 11 May, 1S54, m. Cora E. Phelps. Children by 2nd wife, b. Colebrook, Ct.: 7S91. II. Elizabeth M., b. 4 August, 1864, m. Luther N. Smith, 5 March, 1S85. 7892. III. Edward M., b. 23 May, 1871. 7893. IV. Bertha S., b. 24 Sept., 1842. 4120. Elijah Phelps Grant, b. Colebrook, Ct., 23 August, 1808, m. Susan Boyd, 7 Sept., 1836. She was daughter of James and Mary (Munroe) Boyd, and was b. Winchester, Ct., 19 March, 18 15, d. Omaha, Neb. Mr. Grant was a lawyer, residing at Canton, Ohio. He d. 20-21 Dec, 1874. He graduated at Yale College, 1830. Children, b. Canton, Ohio : 7S94. I. Elizabeth (Grant), b. 21 May, 1838, m. Thos. Hurford, 18 May, 1858. Res. Pueblo, Colo., 1897. 7895. II. Susan (Grant), b. 8 Jan., 1841, d. 19- July,. 1 841. 7896. III. Mary (Grant), b. 12 Sept., 1S42, m. William Wallace. Res. 2420 Henry Street, Omaha, Neb. 7897. IV. Charles Fourier (Grant), b. 12 August, 1844, d. 25 May, 1845, Wheeling, W. Va. 7898. V. Jane (Grant), b. 27 Nov., 1S46, m. Munroe. Res. 1897, 6238 Windsor Ave., Chicago, 111. 7899. VI. Martha A. (Grant), b. 30 April, 1849, d. 27 June, 1859. 7900. VII. James Boyd, b. 10 Nov., 1853. A banker. 4121. Mary Zilpha Grant, b. Colebrook, Ct., 18 Aug., 181 1, 111. Rev. Ebenezer Burgess, 11 March, [839. He was son of Ebenezer and Mary (Hall) Burgess and was b. Grafton, Yt., 26 June, 1805. Graduated at Amherst College in 1831, and at Andover Theological Seminary [837. Was a member of the Mahratta Mission, India, for which lie em
- 37. Seventh Generation. y6^ barked at Salem, i April, 1839, arriving at Ahmednugger the same year, where his wife d, 24 Sept., 1842. (Hem. 2nd Abigail Moore, 15 Sept., 1S46, who d. at Mahavnlishwer, 26 April, 1853, By her he had 1st Mary Porter, b. 11 July, 1S4S ; 2nd Abb}^ Lyon, b. 9 Sept., 1849 ; 3rd Sarah b. 19 April, 1853, died at sea, 17 June, 1854.) Child, b. Ahmednugger, India : 7901. I. Edwin (Burgess), b. 21 June, 1840, died 11 Sept., 1844. 4122. Elizabeth Grant, b. Colebrook, Ct., 8 Feb., 1S13, m. Rev. William Burton, 12 Sept., 1836, who d. 12 March, 1S5S. Resided in East Liberty, Pa., Piketon and Austinburgh, Ohio. She d. 5 April, 1885, or 31 March. (He m, 1st Margaret Hughes in 1824.) Children : 7902. I. Mary Elizabeth (Burton), b. East Liberty, Pa., 23 Dec, 1838, m. Giles Surtleff. Res. Oberlin, O. 7903. II. Philander (Burton), b. Piketon, Ohio, 27 Sept., 1840. Res. Gwinnell, Iowa. 7904. III. Edward (Burton), b. Piketon, Ohio, 8 Oct., 1842. Res. Lincoln, 111. 7905. IV. William (Burton), b. Piketon, Ohio, 9 July, 1S44. Res. Cal. 7906. V. Theodore Elijah (Burton), b. Austinburgh,. Ohio, 20 Dec, 1850. 4123. Rev. Joel Grant, b. Colebrook, Ct., 24 Jan., 18 1 6, m. Abigail Fidelia Cowles, 12 Oct., 1845. She was daughter of Moses and Hannah (Betts) Cowles, and was b. Norfolk, Ct., 13 August, 1820, and d. Bristol, 111., 5 May, 1881. Rev. Mr. Grant graduated at Yale College 1S38, taught a year in Berlin, Worcester Co., Md., professor of mathematics, U. S. Navy, 1839 to 1842, studied theology at Andover, Mass., one year, at New Haven two years, completing the course in 1845, licensed to preach 1 August, 1S44, by New Haven west association, ordained in the north consociation of Litchfield, Ct., 29 Sept., 1845, as an evangelist with the work of mis
- 38. y 6 4. Seventh Generation. siouary in the Valley of the Mississippi. Was pastor of Congregational Church of Dockport, 111., 24 Oct., 1852, to 17 Oct., 1858, supplied Congregational church at Bristol, 111., 26 Dec, 1858, to 1 April, i860, then again to Dockport, 111., from 9 Sept., i860, to 1 August, 1 86 1. Chaplain 12th 111. Infantry 1 August, 1 86 1, to muster out of regiment 12 July, 1865. Chaplain of 113th U. S. Col. Infantry, 14 Oct., 1865, to muster out of'regiment 9 April, 1866. Supplying Congregational church in his native town ten months in 1867, he then returned to the Congregational church of Bristol, 111., where he d. 31 Oct., 1873. Children, b. Dockport, 111.: 7907. 1. John Cowles (Grant), b. 21 April, 1848, graduated Yale, 1869. Res. 2101 Indiana Ave., .Chicago, 111. 7908. II. Mary Hannah (Grant), b. 18 June, 1S51, d. 11 August, 1853. 7909. III. William Elijah (Grant), b. 31 Jul}', 1852, mini., d. 16 Oct., 1S69, Lockport, 111. 7910. IV. Robert Stuart (Grant), b. 5 Oct., 1S57, d. 7 Dec, 1858. 4124. Daniel Grant; b. Colebrook, Ct., 19 June, 1818, m. Caroline Burr, 10 May, 1843. She was daughter of Ebenezer and Permelia (Benton) Burr, and was b, Norfolk, Ct., 22 Feb., 1820, d. New Marlborough, Mass., 7 Sept., 1892. Mr. Grant settled in Waukesha, Wis., as a farmer, resided for a time in Kansas, went to California in 1849, where he continued in the same business and digging for gold till 1856, when he returned to Litchfield, Ct., and farmed in Norfolk for four years, after which he was in trade in stoves and tin ware in West Winsted, Ct. In 188S they were living in New Marlborough, Mass., where he d. 2 Jan., 1892. Children: 791 1. I. Abigail Elizabeth (Grant), b. Waukesha, Wis., 1 August, 1845, m. Rev. Almon W. Burr, Beloit, Wis.
- 39. Seventh Generation. 7657912. II. Edward Burr (Grant), b. Waukesha, Wis., 1 Oct., 1S4S, m. Lucy Churchill Baldwin, 23 March, 18.81. She was b. Southfield, Mass., 21 Sept., 1853. 4125. John Grant, b. Colebrook,Ct.,29 Aug., 1S22, i±i. Sarah Gertrude Day, at Canton Ohio., 20 August,. 1S57. She d. Colebrook, Ct., 1 July, 1881. Mr. Grant graduated at Yale 1845, a tutor in 184S and 1S50, since which engaged in teaching when his health would allow. He d. Colebrook, Ct., 5 July, 1878. Children : 7913. I. Gertrude Elizabeth (Grant), b. 7 Oct., 185S, d. 26 Oct., i860. 7914. II. William Marcus (Grant), b. Oct,, 1S60. 4132. Issac Mortimer Griswold, b. Windsor, Ct., 23 Dec, 1803, m. Blendon, Ohio, 4 Sept., 1833, Mary Osboru, b. Blendon, O., 28 Nov., 18 10, d. Westerville, O., 12 Jan., 1892, dau. of George and Miriam (Palmer) Osborn. Mr. Griswold settled 1806, in Blendon, Ohio, and Winchester, 111., where he died, May, 1839. He was a farmer and fur dealer. Children, b. Blendon, Ohio : + 7915. I. Rosaltha (Griswold), b. 10 April, 1835, m. Daniel M. Slyh. + 7916. II. Minerva (Griswold), b. 18 May, 1839, m. twice. 4134. Christina Griswold, b. Blendon, Ohio, 23 Feb., 1808, m. Thomas Schrock, 8 June, 1S32. Settled in Blendon, Ohio. Children, b, Blendon, Ohio: 7917. I. Isaac (Schrock), b, Nov. 1S33. 7918. II, Orintha b. May, 1S36. 7919. III. Ursula b. Jan., 1S39, m. Had two children. 7920. IV. Edith b. Oct., 1S49. 7921. V. Virginia b. May, 1852,
- 40. 7923II. 7924Ill 7925IV. 7926. •v, j 66 Seventh Generation. 4137. Mindwell E. Griswold, b. Blendon, Ohio, 9 Oct., 1822, m. 9 March, 1841, George Clark, d. Keosaugua, Iowa. Settled in Blendon, Ohio, and Keosauqua, Iowa. After her husband's death she returned to Blendon, Ohio, and having inherited, with her children, the estate of her brother, Cicero P. Griswold, now resides on the homestead in Blendon, Ohio. Children, b. Blendon, Ohio : 7922. I. Louise M., (Clark), b. 23 August, 1842, m. H. Warren Phelps, See No. 4181. Eunice Griswold (Clark), b. 8 April, 1844. George Walter b. 4 Oct., 1S4S. Emma b. 27 Jan., 1849. Charles b. 5 Oct., 185 1. 4137aa. Aurelia Adaline Hempstead, b. Blendon, Ohio, March 25, 1S22, d. Wellington, Ohio, Feb. 9, 1899, m. Sept. 25, 1S51, Orlando Barker, b. Peru, Mass., Dec. 10, 1S10, d. Wellington, Ohio, Marcl 3, 1876, sou of Thomas and- Elizabeth (Fuller) Barker, who were among the pioneer settlers of Lorain County, Ohio. Both Mr. and Mrs. Barker were buried in Huntington, O. Mrs. Barker received a thorough education at Central College, Ohio, Academy, and taught school for many years. Mr. Barker was a carpenter and farmer. Res. Huntington and Wellington, Ohio. See Additions and Corrections for parentage, Children, b. Huntington, Ohio, except last two : 7926a. I. Dwight (Barker), b. 13 August, 1852, d. May 3i, 1853. 7926b. II. Monroe (Barker), b. 21 Dec, 1843, d. 10 June, 1 86 1. 7926c. III. Wallace (Barker), b. 9 July, 1857. + 7926d. IV. Charles Phelps (Barker), b. 25 May, 1S59, m. Laura Butler. 7926c V. Ardelia Lee (Barker), b. Wellington, Ohio,2i Jan., 1864. She graduated at Oberlin College, 18S6, and is now a school teacher. 7926L VI. John Craig (Barker), b. Wellington, Ohio, 21 Feb., [866.
- 41. Seventh Generation. j6~ -t 1 3 7 bb . John Craig Hempstead, b. Blendon, Ohio, 29 Oct., 1S53, d. Topeka, Kanrsas, 22 May, 1892, m. at Des Moines, Iowa, 11 Jan., 1S51, Ellen E. Ramsey, b. Des Moines, Iowa, 14 June, 1S31, d. Nevada, Iowa, 12 Oct., 1880. Mr. Hempstead was a school teacher, accountant and farmer. Res. Blendon, Ohio, Nevada and Des Moines, Iowa, and Topeka, Kansas. Child, b. Nevada, Iowa : + 79.26g. I. Charles William (Hempstead), b. 24 Aug., 1S56, 111. Minnie May Fersou. 4-137e. Alexander S. Hempstead, b. Blendon, Ohio. 27 August, 183S, m. at Lewis Center, Ohio, 22 Oct., -1S66, Mary Prout Lewis, b. Essex Co,, N. J., 3 Nov., 1840, dau. of William Towney and Elizabeth (Fowler) Lewis, of Newark, N. J. Res. Westerville, Ohio. Mr. Hempstead was educated at Central College, Franklin Co., Ohio, Academy, and studied law, and has been a Justice of the Peace. He served three years in the Union army in the war of 1S61. Children, b. Lewis Center, Ohio, except the first : 7927. I. Marion (Hempstead), b. Adel, Iowa, 23 Nov., 1867. A clerk. 7928. II. William Emmett (Hempstead), b. 12 Feb., 1S47. An editor. 7929. III. Grace (Hempstead), b. 12 April, 1877. A teacher. 7930. IV. Don Carlos (Hempstead), b. 20 August, 1S79. A clerk. 4138. Gustavus Swax Phelps, b. Blendon, Ohio, March 30, 1818, d. Placer County, Cal., Jan. 31, 1871, m. Nov. 19, 1S38, Sarah M. Rugg, b. Dec. 16, 1S18, d. Columbus, Ohio, Feb. 22, 1S5S, daughter of Moses and Isabel (Nicholson) Rugg, He m. 2nd, at Madisonville, Mo., Jan. 26, 1S61, Sarah Elizabeth Smith, b. Pike Co., Mo., April 30, 1840, dau. of Matthew M. and Susan (Lane) Smith. Res. Blendon and Columbus, Ohio, and Madisonville. Mo. Mr. Phelps was a farmer and horse dealer.
- 42. The text on this page is estimated to be only 26.13% accurate j6S Seventh Generation. Children by ist wife, b. Blendon, Ohio: -1-7931. I. Rosetta, b. March 30, 1S40. m. Robert D. Dean. 7932. II. Moses T., b. Oct. 14, 1S41, d. March 21, 1845. 7933. III. Edward Meredith, b. Sept. 26, 1843, d. Sept. r5i lS537934. IV, Infant, b. April 11, 1S45, d. April 14, 1845. 7935- V. Mary Isabel, b. Sept. 24, 1846, d. West Union, Iowa, July 14, 1S96, m. June 24, 1S73, Benjamin Heiserman. 7936. VI. Josephine Rugg, b. July 5, 1S50, d. May 12, 1870. Children by 2nd wife : 7937. VII. Everett J., b. Pike Co., 111., Nov. 13, iS6i, d. Sept.. 1S62. + 793S. VIII. Lorena Frank, b. Ralls Co., Mo., June 1, 1865, m. Durham. + 7939. IX. Homer Gustavus, b. Ralls Co., Mo., August 27, 1S66, m. . -j- 7940. X. Martha Mazie, b. Yolo Co., Cal., June 20, 1869, m, Dr. Sauford, 7941. XL Carrie Dee, b. Lincoln, Cal., Feb. 20, 187 1. A school teacher at Red Bluff, Cal. -±139. William Jamisox Phelps, b. Blendon, Ohio, 4 Dec, 1819, m. 4 Feb., 1851, Caroline Hart, b. Oct. 9, 1826, d. Dec. 30, iSyf , daughter of Gideon W. and Xancy (Langdon) Hart. Mr. Phelps settled in Blendon, Ohio, on part of his father's farm. He has been an active business man, an extensive farmer, and has held main' offices of public trust. Children, b. Blendon, Ohio: + 7942. I. Frank, b. 19 Jan., 1852, m. Cora Dell Xoble. + 7943. II. Edward, b. 27 August, 1 s 5 3 , m. Lydia M. Dei bier. + 7944. III. Leonard, b. 23 June, [855, m. Laura Lan don. 4140. Perry Robert Phelps, b. Blendon, ()., 27 July, 1821, d. 14 Feb., 1897, ra. 16 Dec, 1841, Lauretta
- 43. Seventh Generation. j6g M. Wright, b. Bleudon, Ohio, 17 July, 1S20, d. Blendon, Ohio, 16 March, 1S54, dau. of Cruger and Abigail (Goodrich) Wright. He m. 2nd at Blendon, Ohio, 2S April, 1S55, Ann McWhirk, who d. 17 Dec, 1858, He m. 3rd S March, 1S60, Priscilla Decker, of Genoa, Ohio. Res. 1 154 Highland Street, Columbus, Ohio. Mr. Phelps was a farmer and traveling salesman. He was buried in Green Lawn cemetery, Columbus, Ohio. Children by 1st wife, b. Blendon, Ohio : 7945. I. Alice, b. 21 Sept., 1842, d. 23 Jan., 1843. + 7946. II. Chauncey Wright, b, 11 March, 1S44, m. Jane Jones. 7947. III. Cruger, b. 9 April. 1848, d. 27 June, 1848. + 7948. IV. Dayton Rugg, b. 25 April 1850, m. Eaura Patterson. + 7949. V. Kate, b. 23 May, 1852, m. Jonas Concklin. Children by 2nd wife, b. Blendon, Ohio : 7950. VI. Charles, b. 14 Sept., 1S56. 7951. VII. Elizabeth, b. 16 Nov., 185S. Children by 3rd wife, b. Columbus, Ohio : 7952. VIII. Ada, Adelaide, b. 2 Jan., 1861. 7953. IX. Wilhelmiua A., b. 8 May, 1862, m. John Keneer. Res. Chicago, 111. 2 children. 7954. X. Robert F., b. 23 April, 1864. 4-14:1. Harriet Azubah Phelps, b. Blendon, Ohio, 18 May, 1823, d. Sioux Falls, Dak., May, 1S89, m. 9 July, 1S46, Porter Webster Benton, b. Springfield, Mass., 17 April, 1S22, d. Homer, Ohio, 12 June, 18S6, son of Eliakim and Elizabeth (Meacham) Webster) Benton. (Elizabeth Meacham was daughter of Paul and Roxanna (Phelps) Meacham No. 1058. See also additions and corrections. Mr. Benton was buried at Sioux Falls, Dak. They had lived in Quiucy, 111., Sunbury and Blendon, Ohio, and Homer, 111. Children : + 7955. I. Thomas Kerr (Benton), b. Quincy, 111., 14 Feb., 1847, m. Helena Postlewaite. (49)
- 44. 7956. II. 7957Ill, + 7953. IV. + 7959V. + 796o. VI, 7961. VII, jyo Seventh Generation. Betsey Ann (Benton), b. Sunbury, Ohio, 8 August, 1848. Augustus Porter (Benton), b. Sunbury, Ohio,. 20 July, 1850. Mary Aurelia (Benton), b. Blendon, Ohio, 13 Sept., 1 85 2, m. Cyrus Walts. L,ucina Adelia (Benton), b. Blendon, Ohio, 28 Feb., 1S54, m. Henry M. Avery. Harriet Orintha (Benton), b. Blendon, Ohio, 15 Jan., 1S56, m. Menza L. Ashley. Edwin Eliakim (Benton), b. Blendon, Ohio, 12 March, 1858. + 7962. VIII. Angeline Roxanna (Benton), b. Homer, 111., 2 July, 1S61, m. John H. Duling. 414:3. Margaret Jane Phelps, b. Blendon,. Ohio, 3 March, 1S28, m. 22 Feb., 1844, Joseph C. Vance, b. , d, June 23, 1882, 'son of Joseph and Cynthia (Hart) Vance. Settled in Blendon, Ohio, in 1841 Was a farmer. Children, b. Blendon, Ohio, except 3rd and 4th: 7963. I. George Alexander (Vance), b. Oct. 3, 1S45, d. Sept. 18, 1893. 7964. II. Edward Phelps (Vance), b. April 5, 1S47, m. Lillian New comb, July 7, 1SS7. 7965. III. Juliann (Vance), b. Frankliuton, O., Feb. 12, 1849, m. June 29, 1875, Vance Roberts, her cousin . + 7966. IV. Cynthia Ellen (Vance), b. Frankliuton, O., August 20, 1S50, m. William W. Hutchisson. 7967. V. Joseph (Vance), b. May 20, 1S53, d. August 8, 1853. 7968. VI. Lizzie (Vance), b. June 25, 1854. + 7969. VII. Robert Cutler (Vance), b. Feb. 10, 1856, m. Jane Douglass. + 7970. VIII. John Joseph (Vance), b. June 7, 1858, m. Donna Wilkins. 7971. IX. Abraham Lincoln (Vance), b. July 30, 1S60,. d. Jan. 30, 18S5.
- 45. Seventh Generation. 7JT + 7972. X. Timothy (Vance), b. Nov. 29, 1864, m. Mary Johnson. + 7973. XI. Clinton Henry (Vance), b. June 22, 1S66, m. Lulu P. Landou. 41-14. Timothy Denton Phelps, b. Blendon, Ohio, Sept. 12, 1S30, m. Dec. 3, 1850, Clarissa Pinney, b. Blendon, Ohio, Dec. 7, 1830, d. Blendon, Ohio, June 12, 1S68, daughter of Grove and Mary (Cummings) Pinney, of Conn. and Blendon, Ohio. He m. 2nd, Sept. 22, 1S69, Zelda Andrews, b. Worthington, Ohio, May 12, 1833. Res. Blendon, Ohio, and Kansas Cit}*, Kansas. Mr. Phelps is a farmer, live stock dealer and traveling salesman . Children by 1st wife, b. Blendon, Ohio: + 7974. I. Alice Jennie, b. Sept. 3, 1S51, m. James R, Fisher. 7975. II. Albert Larey, b. April 2, 1S53, d. July 10, 1S54. + 7976. III. Jessie Adel, b. July 9, 1855, m. Edwin M. Jocelyn. + 7977. IV. Clara Josephine, b. Jan. 22, 185S, m. Arthur H. Jones.' + 797S. V. Hector Melville, b. April 23, 1S60, m. twice. + 7979. VI. Elmer Ellsworth, b. August 3, 1863, m. Mary R. Goodrich. Child by 2nd wife, b. Blendon, Ohio: + 7980. VII. Timothy Denton, b. Oct. 9, 1871, m. Jennie E. McGee. 4145. Homer H. Phelps, b. Blendon, 0.„ 8 Xov., 1S32, m. Mar}- Merrill, 24 Nov., i^~n Mr. Phelps settled in Iowa. Children, b. Iowa: 7981. I. Francis E., b. 9 Sept., 1854. 7982. II. Florence L., b. 14 August, 1855. 4146. Lauretta Clarissa Phelps, b. Blendon, Ohio, Sept. 18, 1835, m- Xov. 24, 1853, Xathan S. Vincent.
- 46. 77 Seventh Generation, Res. Blendon, Ohio. . Children, b. Blendon, Ohio, except 5th : 7983. I. Charles (Vincent), b, Dec. 9, 1855, m. Nov. 22, 1898, Dora Carnes of Mansfield, O. 7984. II. Chauncey (Vincent), b. Nov. 19, 1S57, d. March 28, 1885, m. Feb., 1884, Hattie Davis. 7985. III. Corwin Thomas (Vincent), b. Dec. 24, 1859, d. March 21, 1895. + 7986. IV. Carrie Ferson, b. Oct. 14, 1862, m. Edward Linnabary. 7987. V. Joseph Clark (Vincent), b. Geneva, Ohio, March 21, 1866, m. July 17, 1S98, Laura F. Hanson, of Wester ville, O. 7988. VI. William Ferson (Vincent), b. Feb. 14, 1868. + 79S9. VII. Fred. Wright (Vincent), b. March 7, 1871, m. Cora Stanton. 7990. VIII. Walter Brown (Vincent), b. July 14, 1873. •4140. Emily Williams, b. Genoa, Ohio, May 26, 1821, d. Feb. 3, 1890, m. George McLeod, b. Genoa, Ohio, Jan. 2, 1816, d. Feb. 22, 1890. Mr. McEeod was a farmer in Genoa, Ohio. Children, b, Genoa, Ohio: + 7991. I. Milton William (McLeod), b. July 2. 1844, m. Mary A. Schott. + 7992. II. Clifford (McLeod), b. May 19, 1846, m. Theresa Jane Wurm. + 7993- III- Miles Clinton (McLeod), b. Sept. 16. 1849, m. Ordelia J. Mahaney. + 7994. IV. Emma (McLeod), b. Oct. 9, 1851, m. Adam Bookman. + 7995- V. Charles (McLeod), b. Oct. 4, 1833, m. Maggie A. Huelf. + 7996. AT. Homer H. (McLeod), b. July 29, 1855. m. Mary A. Mullen. 4151. Amos Williams, b. Genoa, Ohio, Oct. 2, 1823, m. Feb. 4, 1826, Susan Campbell. Res. Genoa, Ohio,
- 47. Seventh Generation. yjj Child : 7997- I. •±152. Edward Phelps Williams, b. Genoa, Ohio, 15 Dec, 1S24, d. 30 April, 1S95, m. 6 April, 1S51, Mary Octavo L-andon, of Blendon, Ohio, b. , d. 24 Aug., 1S94. Children : + 799S. I. Ida May (Williams), b. 1 May, 1852, m. William M. Benton. 7999. II. Inez (Williams), b. 16 August, 1S55, m. James Powell. Sooo. III. Annis Dee (Williams), b. 12 June, 1857. 8001. IV. Edward Virgil (Williams), b. 30 Oct,, 1859. Res. Kankakee, 111, 8002. V. Nina (Williams), b. Genoa, Ohio, 7 Dec, 1865. 8003. VI. John (Williams), b. Genoa, Ohio, 9 July, 1867. Res. Columbus, Ohio. Is in employ of a R. R. Co. 4156. Alma Williams, b. Genoa, Ohio, 22 August, 1S31, m. 3 May, 1866, William H. Grinnell, b. Genoa, Ohio, 1S14, d. 13 August, 1897, aged 83 years. Res. Blendon, Ohio, and Kankakee, 111. Children, b. Kankakee, 111., except the first : 8004. I. Anna P., b. Blendon, Ohio, 19 Dec, 1867. 8005. II. KateR., b. 2 Dec, 1868, m. 10 June, 1896, Allison Gibbs. 8006. III. Grant, b. 16 Sept., 1870. 8007. IV. G. Clark, b. 19 June, 1872, m. 24 Nov., 1898, Anna Bowen. 8008. V. Effa M., b. 8 May, 1876. 4157. Corixtha Williams, b. Genoa, Ohio, 18 Dec, 1832, d. 26 Feb., 1S63, m. 8 Feb., 1S55, Edwin Phillips, son of William and Helen (Bishop) Phillips, of Westerville, Ohio. Children: S009. I. William (Phillips), b. 12 Aug., 1858. Res. Columbus, Ohio. Is in employ of Standard Oil Co.
- 48. 77^ Seventh Generation. 8010. II. Frank (Phillips), b. 3 Dec, 1859. A farmer near Galena, Ohio. Son. III. Charles (Phillips), b, 23 June, 1861. Supt. Industrial Dept., Illinois State Reform School, at Peoria, 111. 4158. Cloe Williams, b. Genoa, Ohio, Feb. 6, 1835, m. May 24, i860, Rodney Romoaldo Cooke, b. Feb. 26, 1832, d. Clintonville, Ohio, Oct. 28, 1886, son of Rodney and Faura (Cowles) Cooke. Mr. Cooke was a farmer. Child, b. Clintonville, Ohio: 8012. I. Fuella Z., b. March 3, 1862. 4159. LUCINDA FORELLY WlLLIAMS, b. Genoa, Ohio, Nov. 1, 1836, m. Dec. 21, 1854, William Penn Vincent, b. , d, , While Mr. Vincent was engaged in raising cotton near Helena, Arkansas, he was taken prisoner August 15, 1864, by Confederate troops, and died while a prisoner. Children: 8013. I. William Dalbert (Vincent), b. Troy, Ohio, Oct, 17, 1857, m. at Pittsburgh, Pa., Nov. 12, 1897, Cecelia Connelly, Res. South Bethlehem, Pa. He is superintendent of lost car tracers for Penn. Central R. R. 8014. II. Jennie Belle (Vincent), b. March 16, 1S60, m. at Delaware, Ohio, Sept. 12, 1884, George Creighton, b. Carlisle, England. Res. Moscow, Idaho. 4160. Virgil Douglass Williams, b. Genoa, Ohio, August 16, 1839, d. June 19, 1875, m. at Galena, Ohio, Dec. 25, 1865, Emmeline Cox, b. , d. March 21, 1S86. Mr. Williams enlisted in Co. D., 20th Reg., Ohio Vol. Inf., Sept. 24, 1 86 1, and served through the civil war. He was in many battles, and at the battle of Champion Hills, in the siege of Vicksburg, May, 1863, received a gunshot wound in the head from which he recovered at the time, but in 1N74 a growth on the brain caused by the wound gave him softening of the brain, from which he died a' year later. He was a farmer.
- 49. Seventh Generation. 775 Children, b. Genoa, Ohio: 4-8015. I. Iono Cox (Williams), b. Jan. 8, 1S6S, m. Ira H. Steele. + S016. II. Bertha Phelps (Williams), b. July 15, 1871, m. George W. Page. 8017. III. Gilbert Hoover (Williams), b. March 12, 1873, d. Jan. 1 1, 1875. 4161. Victor Williams, b. Genoa, Ohio, 16 August, 1S39, m. 12 Sept., 1865, Priscilla Martin, of Genoa, Ohio. Mr. Williams is a farmer in Galena, Ohio. Children, b. Genoa, Ohio : 5018. I. Eucinda (Williams), b. 24 Oct., 1866. 5019. II. Edward (Williams), b. 4 June, i87i,d. 6 June, 1871. 5020. III. Clayton Gilbert (Williams), b. 24 August, 1872, m. Elizabeth Haines. 8021. VI. Effie Fayetta (Williams), b. 8 May, 1S75, m. 29 April, 1897, Charles Franklin McCarty. 8022. V. Virgil Clifton (Williams), b. 31 August, 187S. S023. VI. Clarence Victor (Williams,) b. 31 May, 1884. 4162. Mary Azubah Gillespie, b. Blendon, Ohio, 22 August, 1S1S, m. 4 April, 1844, Ephraim McCleary, of Harrisville, Ohio. Probably had seven children. Mr. McCleary was a farmer and stock raiser in Harrisville, Ohio. Child, b. Harrisville, Ohio : + 8024, I. Clayton (McCleary), b. 19 Sept., 1848, m. Henrietta Holmes. 4163. William Marcus Gillespie, b. Blendon, Ohio, 19 Oct., 1821, m. Blendon, Ohio, 4 Nov., 1S46, Ann Park, b. Philipsburg, N. J., 20 August, 1821, d. Durfee, Ind., 9 Oct., 1S7S, dau. of Abner and Sarah (Pennell) Park. Mr. Gillespie was a farmer in Durfee, Ind., but is now retired, living with his children. He and his wife settled in Jefferson, Ind., in 1848, clearing up land that was heavily wooded. Children, b. Durfee, Ind :
- 50. yj6 Seventh Generation. 8025. I. (Gillespie), d. young. 8026. II. 8027. III. + 8028, IV. Mary Cornelia b. iS Sept., 1856, m. Samuel F. Swayne. + 8029. V. Arthur Marion b. 6 Oct., 1858, m. Barbara Howenstine. + 8030. VI. Morton Abram b. 15 Feb., 1865, m, Ina Smith. 4164. Abram Jabez Gillespie, b. Blendon, Ohio, April 11, 1824, 111. at Blendon, Ohio, May 30, 1848, Amanda D. Jenkins, b. Erie Co., N. Y., Oct. 25, 1824, dau. of Samuel and Margaret R. Jenkins. Mr. Gillespie and famity removed to Jefferson, Ind., in Dec, 1852. He served in the Union army in the war of 1861. He is a farmer and has held many local offices. Is a member of the Methodist Episcopal Church. He owns the sword which his father, Capt. Menzes Gillespie carried in the war of 1812. Res. Uand, Ind. Children : + S031. I. Isora Jenkins (Gillespie), b. Blendon, Ohio, Dec. 15, 1849, m. Louis Balus. + 8032. II. Eudora Azuba (Gillespie), b. Blendon, Ohio, July 30, 1 85 1, m. Theodore Swihart. + 8033. III. Sylvester Menzes (Gillespie), b. Jefferson, Ind., Sept. 6, 1853, m. Annabell Druley. 4165. Alma Ducy GIllespie, b. Blendon, Ohio, July 3, 1827, d. Feb. 9, 1S93, m. Nov. 2, 1847, Moses Taggart Dickey, b. North Greenwich, N, Y., 1823, d. Central College, Ohio, March 12, 1898, son of Joseph and Lovina (Taggart) Dickey, of Greenwich, N. Y. Mr. Dickey came to Blendon, Ohio, in June, 1S3S. He was a farmer. Children, b. Blendon, Ohio: 8034. I. Clarence Walter (Dickey), b. August 31, 1S48, in. April 4, 1X94, Flora Robinson, of
- 51. Seventh Generation. yyj Alton, Ohio. They have two children, He is a clerk in the U. S. pension bureau at Washington, D. C. + S035. II. Alice Lovina (Dickey), b. Dec. 11, 1849, m. John Allen McCoy. S036. III. Alma Gillespie (Dickey), b. Dec. 8, 1851. 8037. IV. Charles Albert (Dickey), b. June 18, 1S55, d. Feb. 26, 1858. + S03S. V. Clayton Leroy (Dickey), b. Jul}- 26, 1859, m. Mary F. Wilbur. 8039. VI. Marcus Courtland (Dickey), b. Sept. 29, 1 86 1, m. at Cincinnati, Ohio, April 26, 1890, Mary Lewis, of Newport, Ky. Res. Columbus, O. Mr. Dickey is a newspaper editor. 4166. Menzes Phelps Gillespie, b. Blendon, Ohio, Jan. 5, 1830, d. Nov. 19, 1896, m. Dec. 21, 1854, Harriet E. Eblin. Mr. Gillespie was a farmer in Ohio, and Mount Vernon, South Dakota. He served in Co. C, 133rd Ohio Vol. Inf., in war of 1861. Children, b. Blendon, Ohio: S040. I. George Walter (Gillespie), b. April n, 1856, d. Sept. 27, 185S. + S041. II. Robert Ernest (Gillespie), b. Jan. 12, 1858,. m. Mary M. Fisher. 4169. Octavius Phelps, b. Blendon, Ohio, 8 June, 1825, m. Eydia Decker, 1 June, 1848. She was b. Penn. and d. Oct. 25, 1883, daughter of Henry Decker. He m. 2nd, March 26, 1885, Martha E. Slater, b. Warren Co., Ohio, March. 5, 1839. Mr. Phelps settled in Jefferson, Whitley Co., Ind. Is a farmer, and has been justice of the peace for many years. Children, b. Jefferson, Ind., by 1st wife: 8042. I. Agues Jane, b. 5 Sept., 1850, m. M. G. Wright. 8043. II. Florence, b. 21 Feb., 1852, m. William Rich. 8044. III. William Henry, b. 21 June, 1854.
- 52. Welcome to our website – the ideal destination for book lovers and knowledge seekers. With a mission to inspire endlessly, we offer a vast collection of books, ranging from classic literary works to specialized publications, self-development books, and children's literature. Each book is a new journey of discovery, expanding knowledge and enriching the soul of the reade Our website is not just a platform for buying books, but a bridge connecting readers to the timeless values of culture and wisdom. With an elegant, user-friendly interface and an intelligent search system, we are committed to providing a quick and convenient shopping experience. Additionally, our special promotions and home delivery services ensure that you save time and fully enjoy the joy of reading. Let us accompany you on the journey of exploring knowledge and personal growth! ebookfinal.com