Methods of strain development by mutation and isolation methods

This document discusses methods for strain development in biotechnology. There are two main methods: mutation and genetic recombination. Mutation involves inducing changes to an organism's DNA through spontaneous or induced means like radiation or chemicals. This can improve strain performance. Selection of mutants involves screening colonies for desired traits like increased product formation. Isolation techniques identify mutants with traits like antibiotic resistance or an inability to synthesize certain factors (auxotrophs). Overall, strain improvement aims to genetically modify organisms for enhanced metabolic functions in biotechnology applications.