Single Cell Protein - Slideshare PPT
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Single cell proteins (SCP) are dried cells of microorganisms that can be used as protein supplements for humans and animals. SCP production was first commercialized in the 1950s using bacteria cultured on methanol. Common microorganisms used for SCP production include fungi, yeast, algae and bacteria. Production involves selecting a suitable microorganism strain, fermenting it under controlled conditions, harvesting the cells, processing them, and isolating the protein. SCP have potential applications as nutritional supplements, health foods, and animal feed due to their protein and nutrient content.
Single Cell Protein - Slideshare PPT
- 1. SINGLE CELL PROTEIN By- Priyabrata Karmakar Couse – Project & Seminar I MSc Microbiology (1st sem) JIS UNIVERSITY
- 2. CONTAINS- Brief description History Used Microorganisms as raw material Production Places Production Steps Uses of SCP Conclusion References
- 3. WHAT ARE SINGLE CELL PROTEINS ? SCP are dried cells of microorganisms which can be used as protein-rich food supplements by humans and animals They are used as animal feed & can be used for human feed as protein supplement. Also called ‘Novel Food’ & ‘Minifood’.
- 4. HISTORY OF SCP • In 1950’s British Petroleum initiated production of SCP on commercial basis. • Pruteen was the 1st commercial SCP used as animal feed additive • Pruteen was produced from bacteria Methylophilus methylotrophus cultured on methanol & had 72 % protein content. • Earlier known as ‘Microbial Protein’. • In1966 M.I.T Professor Carroll L.Wilson renamed it as “Single Cell Proteins”
- 5. MICRO-ORGANISMS USED FOR SCP PRODUCTION Fungi Aspergillus fumigatus Aspergillus niger Rhizopus cyclopium Yeast Saccharomyces cerevisae Candida tropicalis Candida utilis Algae Spirulina sps. Chlorella pyrenoidosa Chondrus crispus Bacteria Pseudomonas fluroescens Lactobacillus Bacillus megaterium
- 6. SCP PRODUCTION IN INDIA • National Botanical Research Institute (NBRI). • Central Food Technological Research Institute (CFTRI) • In CFTRI, SCP is produced from algae cultured on sewage
- 7. PRODUCTION OF SCP 1. Selection of Suitable Strain 2. Fermentation 3. Harvesting 4. Post harvest treatment 5. Processing of SCP for food Single Cell Protein production involves following steps:
- 8. STEP- 1 SELECTION OF SUITABLE STRAIN • It a very critical step as the quality of protein depends totally on the microbe that is used for the production. • Thus careful selection of the strain should be done. • Care should be taken that the selected strain should not produce any toxic or undesirable effects in the consumer.
- 9. STEP -2 FERMENTATION • Is done in a large chamber either of glass or stainless steel called “Fermentor” • Fermentation should be done under sterilized conditions • Controlled conditions as necessary e.g. Temperature, Pressure, pH, Humidity etc • Usually fed-batch cultures are used for the fermentation of microbes
- 10. STEP -3 HARVESTING • When the colonies of microbes are fully developed, they are then harvested • The bulk of cells are removed from the fermenter by decantation.
- 11. STEP -4 POST HARVESTING • After harvesting, the cells are subjected to a variety of processes. • Post harvesting treatments includes steps like separation by centrifugation, washing, drying, etc.
- 12. STEP -5 FINAL PROCESSING OF SCP • Pure protein isolation can be done by disrupting the cell wall through crushing, crumbling, cycles of freezing & thawing, grinding & thermal shocks. • Nucleic acid can be remove by- By treatment with Nacl 10% By Chemicals e.g. NaoH Thermal shocks Enzymes Treatment e.g. ribonucleases
- 13. USES OF SCP • As protein supplemented food -source of vitamins, amino acids, minerals, crude fibers, etc • As health food-controls obesity ,provides instant energy • Therapeutic and natural medicines- reduce body weight, cholesterol, stress , lowers blood sugar level in diabetic • In cosmetics-important role in maintaining healthy hair (vitamin A and B), many herbal beauty products (biolipstics and herbal face cream) • Poultry and cattle feed- excellent, convenient source of protein and other nutrients
- 14. CONCLUSION • At present SCP production is in its infancy. One of the ways to enhance productivity and quality is genetic improvements of micro-organisms. • Using microbial biomass as a food source deserves serious consideration because of insufficient world food supply and high protein content of most microorganisms. • Microbes have shorter generation time, allow easy transformation, utilize many substrates, have no requirements in arable lands or any particular season to grow and have the possibility of continuous production in any part of the world.
- 15. REFERENCE S https://en.wikipedia.org/wiki/Single-cell_protein https://www.sciencedirect.com/topics/agricultural-and- biological-sciences/single-cell-protein https://pubmed.ncbi.nlm.nih.gov/8543324/ https://www2.slideshare.net/FIRDOUS88/single-cell-protein https://www.biologydiscussion.com/single-cell- protein/production-of-single-cell-protein-and-mushrooms/10392