Micropragation
- 1. Application of biotechnology in agriculture Micropropagation By Pradeep Roll no. – 18/5443 Biochemistry (hons) VI semester
- 3. Micropropagation is the production of whole plant from a Small section of plant such a stem tip,node,meristem ,embryo Or even a seed. What is micropropagation? It is also defined as an advanced vegetative propagation technology for producing a large no. Of transplant in a limited time and space.
- 4. Stages Selection of mother plant 1. Establisment Of aseptic culture 2. Multiplication of shoot 3. In vitro rooting 4. Transplantaion or hardening
- 5. Selection of mother plant Selection of mother plant is crucial. Disease free mother explant is selected. High potential to grow and divide.
- 6. 1.Establishment of aseptic culture The main step involves surface sterilization and establishment of explant on culture medium. Plant tissue are commonly associated with bacteria and fungus . Therefore , sterilization method are used to eliminate the Microorganisms fr the explant.
- 7. 2.Multiplication of shoots: The main objective of multiplication of shoot is to give rise to Individual plant. • This involve inoculation of explant in Suitable culture media And proliferation or multiplication of shoot from the explant on media. • Musashige and skoog (MS) medium used as cultivation. • The Innoculum are incubated At optimum temperature during this period shoot will be obtained. • The shoot Can be transferred into Shoot inducing media and incubated to obtain multiple shoot.
- 8. 3. In vitro rooting: Shoot or plantalet obtain in stage 2 Not contain roots amd fails to grow in soil.Therefore, adequate step are taken to grow individual plantlet that can carry out photosynthesis and survive without supply of carbohydrate.therfore, in vitro grown shoot must bhi transfer in rooting media . In vitro rooting can be accomplished by adding auxins to the culture media. Supplying riboflay and other adjuvant enhances root induction process.
- 9. 4. Transplantation or hardening: Process of transferring the plantlet from aseptic culture medium to soil. The lab grown plantlet may fail to survive in soil due to desication, unfavourable condition and due to unadjustability from dependent to independent nutrition. Therefore, acclimatization is needed before cultivation in soil. Acclimatization can be done – by exposing the test tube culture to unfavourable environment before first two week of planting outside. functional leaf by inducing functional root
- 11. How does Micropropagation work? Plant cell have ability to reproduce the whole plant from single cell.this is called totipotency. Totipotency is The ability of single cell to express the full genome in the cell to which it give rise by cell division.
- 12. Why do we do it? To regenerate plant from single cell or Plant tissue. To produce large quantity of identical plants To create new plant varieties. The Micropropagation method produce disease free plant. Help to save endangered species.
- 13. Thank you