Mid-Term-Bioleaching-Presentation-2015
- 1. Justin Rangasawmy & Kusum Basnet ENGI33126 – Tech. Report 2
- 2. Definition of bioleaching Different methods of used in experiment: Acid leaching vs. Bioleaching Purpose and general overview Problems and solutions Quality Control Method Next presentation
- 3. Extraction of metal from ore using non traditional methods Metallic ores contain metal sulphides. Leaching converts metal sulfide to a metal forms which can be solubilized and filtered.
- 4. Bioleaching Acid Leaching Industrial Leaching
- 5. Definition: Bioleaching is a method of metal extraction of ore with the usage of microorganisms. Examples: Bacteria: Paenibacillus Polymyxa Fungi : Aspergillus niger Penicillium simplicissimum
- 6. Definition: Acid leaching is a wet method of metal extraction where ore is treated with acid and agitated for efficiency. Goal is to oxidize the original metal form to a water soluble form.
- 7. In Bioleaching: Grow bacteria, Paenibacillus Polymyxa, to extract a variety of metals from Olivine ore. Comparative experiment – leached metals using bioleaching will be compared to acid leach.
- 8. In Acid-leaching: Usage of Lactic and Hydrochloric acid to extract a variety of metals from olivine ore. Comparative experiment of Acid-leaching of Olivine ore from various locations: Spain, Italy and Norway
- 9. Bioleaching and Acid-leaching: Metals extracted will be analyzed for mass amounts using ICP-OES analysis.
- 10. Streak the plates with revived bacteria. Leave plates in incubator for growth.
- 12. Mature bacteria colonies were introduced to liquid broth sample along with pulverized ore.
- 13. Left to leach for 2 – weeks in incubator
- 15. The Bioleaching sample triplicates were centrifuged.
- 17. Live bacteria were killed off using the autoclave.
- 18. Acid leaching was also done on the ore for comparison of results. Both LacticAcid and Hydrochloric Acid were used in leaching process. Left for 1 week on shaker table and later centrifuged.
- 20. 5 standards were made for calibration of the instrument. 24 samples were made up for acid leaching. 3 unknown replicates were made from bioleaching process.
- 22. Standards, samples and unknowns were analyzed.
- 24. Originally, fungi, Aspergillus Niger was proposed Aspergillus Niger: Important organism in biotechnology Used in enzyme production A promising Nickel leaching micro-organism
- 25. Problem? A. Niger contains toxic spores than can be airborne Can make people with weak immune systems become very sick may even result in death Can cause a lung disease calledAspergillosis
- 26. Solution? Bacteria, Paenibacillus Polymyxa was used Seemed safer alternative. Soil bacteria Contributes in nitrogen fixation
- 27. Contamination of agar plate While incubating agar plates it was discovered that different colonies of micro- organisms where growing along
- 28. Description: Rod shaped Gram positive Colorless
- 31. Blank AgarTest
- 33. Source? Shared micro-lab Fridge Sponge plug Desk Surface
- 34. Solution? New agar solution was prepared Autoclaved equipment Use of biosafety cabinet Used UV light to disinfect surface
- 36. Kusum working in biosafety cabinet
- 38. Bacteria when received were freeze dry Growth and viability of bacteria was important Temperature control and PH testing were done
- 39. pH strips were used for pH testing
- 40. Experimental Results Waste/Disposal Equipment Used (ShakerTable, Incubator, Bio-Safety Cabinet etc.) Case Study on Bioleaching – Current Research Projects. Paenibacillus Polymyxa Overview
- 41. Questions????