Mohammed Said Project Defence PowerPoint presentation
- 1. Presented by : MUHAMMED SAID MUHAMMED 20/03/01/024 Supervised by : PROF. MUSTAPHA BALA ABUBAKAR Department of Biological Science, Biotechnology, Borno State University . December, 2024 SEROLOGICAL EVIDENCE OF INFECTIOUS BURSAL DISEASE IN CHICKEN
- 2. INTRODUCTION • The global poultry industry, including Nigeria, faces significant threats from IBD, resulting in substantial economic losses (van den Berg, 2000) • Young chickens, typically between 3-6 weeks old, are highly susceptible to IBDV, which causes a severe and contagious disease (Eterradossi and Saif, 2008) • IBD outbreaks have devastating economic consequences, including significant losses due to mortality, morbidity, and reduced vaccine effectiveness (Heise et al., 2015) • Detecting IBDV antibodies is essential for diagnosing and monitoring IBD outbreaks in Nigeria (Eterradossi and Saif, 2008)
- 3. Infectious bursal disease virus, chicken Enlarged cloacal bursa, infectious bursal disease virus, chicken Atrophic cloacal bursa, infectious bursal disease virus, chicken
- 4. STATEMENT OF PROBLEM Infectious Bursal Disease (IBD) is a highly contagious viral infection that primarily affects young chickens, leading to considerable economic losses in the poultry industry due to increased mortality, decreased productivity, and immune suppression. In regions like Maiduguri, where poultry farming is a vital source of livelihood, the prevalence of IBD poses a serious threat to food security and the local economy.
- 5. AIM AND OBJECTIVES OF THE STUDY Aim The aim of this study is to investigate the seroprevalence of Infectious Bursal Disease (IBD) in chicken populations in Maiduguri, Nigeria. Objectives 1. Determine the seroprevalence of IBD antibodies in chicken populations. 2. Identify and characterize IBDV strains circulating in the study area. 3. Evaluate the efficacy of current IBD vaccines.
- 6. METHODOLOGY Study Area and Sample Collection • Study conducted in Maiduguri, Nigeria • Sample collection sites: Monday Market, Yan Kaji Area, Damboa Road, and Gidan Madara • Total samples: 100 Sample Preparation and Analysis • Samples collected using sterile tubes • Samples processed through centrifugation and serum preservation • Gel medium prepared using agarose gel and other chemicals • Serological analysis conducted using Ouchterlony technique Serological Analysis • Serum samples dispensed into wells • Antigen and control placed in central well • Plates incubated
- 7. Serial No. Age (Weeks) Gender No. of Samples tested No. of (%) Positive 1. 12 – 24 Female 10 6 (6%) 2. 20 – 28 Male 08 4 (4%) 3. 24 – 32 Male 09 3 (3%) 4. 14 – 38 Female 07 5 (5%) 5. 26 – 46 Female 10 6 (6%) 6. 18 – 30 Female 10 4 (4%) 7. 11 – 22 Male 09 7 (7%) 8. 10 – 20 Female 10 9 (9%) 9. 13 – 25 Female 09 3 (3%) 10. 17 – 34 Male 10 5 (5%) 11. 20 – 29 Male 08 6 (6%) - - 100 57 (57%) Summary results of presence of IBD antibodies in chicken Sera, collected from various locations in Maiduguri. The serological study revealed a 57% seroprevalence of IBD antibodies in chickens sampled from various locations in Maiduguri. The data suggests that a significant proportion of the chicken population in the area has been exposed to IBD. RESULT
- 8. CONCLUSION AND RECOMMDATION Conclusion A recent study investigated the presence of Infectious Bursal Disease (IBD) antibodies among chickens in Maiduguri, Nigeria. The study revealed a 57% prevalence of IBD antibodies, indicating widespread exposure and a potential risk for transmission to other poultry species. Key Recommendations ① Implement regular serological testing every 3-6 months to monitor IBD prevalence trends ② Vaccinate chickens against IBD, targeting birds under 6 weeks old ③ Educate farmers and poultry owners on IBD risks, transmission, and biosecurity practices through training programs ④ Enforce strict biosecurity measures, including quarantine, hygiene, and restricted bird movement ⑤ Conduct further research on the genetic diversity of IBD virus strains and transmission dynamics between chickens and other avian species
- 9. Abdel-Alim, G. A. and Saif, Y. M. (2001): Immunogenicity and antigenicity of very virulent strains of infectious bursal disease viruses. Avian Diseases. 45, 92-101. Abdel-Alim, G., Awaad, M.H. and Saif, Y.M. (2003). Characterization of Egyptian field strains of infectious bursal disease virus. Avian Diseases, 47:1452-1457. Abdu, P.A., Umoh, J.U., Abdullahi S.U and Saidu, L. (2001). Infectious Bursal Disease (Gumboro) of chickens in Nigeria.TropicalVeterinarian, 19 (4):216-236. Adamu J., Owoade, A.A., Abdu, P.A., Kazeem, H.M and Fatihu, M.Y. (2013). Characterization of field and vaccine Infectious bursal disease virus from Nigeria revealing possible virulence and regional markers in the VP2 minor hydrophilic peaks. Avian Pathology, 42, (5), 420-433. Adamu, J., Abdu, P.A., Owoade, A.A., Kazeem, H.M. and Fatihu, M.Y. (2011b). Preliminary trial of a killed infectious bursal disease vaccine developed using Nigerian field vvIBDV isolate. Zariya Veterinarian, 8, 15-20. REFERENCES