Myco ppt
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Mycoplasmas are the smallest free-living organisms lacking a cell wall. They range from 0.2-0.3 μm in size and are bounded by a single membrane. Mycoplasmas are pleomorphic, occurring in coccoidal or filamentous shapes. They reproduce by binary fission or budding and are resistant to some antibiotics like penicillin but sensitive to tetracycline and chloramphenicol. Mycoplasmas require rich media with sterols and serum for growth at 35-37°C and form small transparent colonies visible under low magnification.
Myco ppt
- 2. GENERAL FEATURES 1. Mycoplasmas are unicellular, smallest, non- motile, free-living organisms and lack a cell wall. unicellular, smallest, non-motile and prokaryotic organisms 2. Bounded only by a single triple layered unit membrane. 3. Size ranges from 0.2-0.3 µm(bacteria 2-10 µm) 4. They are pleomorphic in shape. they occur as two different structural forms during a life cycle: coccoidal, a spherical or spheroidal shape, and filamentous, resembling rods.
- 4. No flagella, no spore and Gram negative. contain both RNA and DNA as well as ribosomes. resistant to antibiotics like penicillin, cephaloridine, vencomycin etc. But sensitive to tetracycline & chloramphenicol. Reproduction by binary fission and budding. Earlier it was called as pleuropneumonia-like organisms or PPLO because the first organism of this mycoplasma species was associated with pleuropneumonia of cattle.
- 5. CULTURE CHARACTERISTICS Most mycoplasmas require a rich medium(liquid or solid)containing a sterol and serum proteins for growth. Temperature 35-37 °C. On solid media, they form minute, transparent colonies. When viewed under low-power magnification, the colony looks like a “fried egg”. The different strains vary in their growth rate and may take from two days to several weeks to form a colony.
- 6. Do not stain with the Gram stain. With Giemsa stain, they appear as tiny pleomorphic cocci, short rods, short spirals, and sometimes as hollow ring forms.
- 9. Mycoplasma pneumoniae- respiratory infection Mycoplasma genitalium- causes urethritis and pelvic inflammation. Mycoplasma hominis- urogenital infections. etc INFECTIONS CAUSED BY MYCOPLASMA-
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