Mycobacterium.ppt
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This document discusses the diagnosis of Mycobacterium tuberculosis. It notes that M. tuberculosis is an aerobic, acid-fast bacillus that is transmitted via aerosols and causes tuberculosis (TB). Diagnostic tests for TB include culturing samples, detecting the bacteria in smears of respiratory secretions, chest X-rays, tuberculin skin tests, and molecular tests. The diagnostic procedure involves decontaminating pulmonary or extra-pulmonary samples, making smears, and inoculating samples onto Lowenstein-Jensen and MGIT media to culture for up to 42 days to detect potential positive cases.
Mycobacterium.ppt
- 2. Introduction Pathogenic bacteria of mycobacteraceae family Aerobic bacilli –non spore forming non motile Cell wall –rich in lipids Acid-fast bacilli Very slow growing Route of transmission- aerosole
- 3. TB drug resistance: resistant to one or more of first line drugs: isoniazid, rifampin, pyrazinamide and ethambutol (~ 5% in Canada) MDR-TB: resistance to at least INH and rifampin (~ 0.7% in Canada) XDR-TB: resistance to all first line therapeutic drugs (rare)
- 4. Diagnostic Testing Culture is still the recommended method for diagnosing TB Detection of AFB in respiratory secretions Chest X-ray Tuberculin skin test (TST) Molecular diagnostic tests
- 5. procedure Pulmonary or extra pulmonary samples are first decontaminated (NAL soln.) Smear formation Inoculation to LJ and MGIT media.
- 7. After inoculation of specimen the MGIT are kept in bactec for 42 days If any tube appears positive, It should be sub cultured, acid fast stained and treated as presumptive positive
- 11. thank you