![Tinfoil Barb [TFB]
§ Globally popular aquarium Cyprinid
§ Native to Southeast Asia
§ No authentic records on its natural
spawning in captive condition in Sri
Lanka
Objective of breeding TFB
§ Inducing the spawning of captive reared TFB using
Ovaprim
§ Introducing less time consuming and fruitful breeding
method for commercial scale fish breeders in Sri Lanka](https://image.slidesharecdn.com/5b903481-7af9-43b1-9a62-0e92974b4b4a-160622065231/85/NSS-2016-ppt-2-320.jpg)
![Materials and methods
Inducing agent - Ovaprim
— Uses to induce ovulation and
spermiation in fish
— A synthetic GnRH [1 mL
contains 20 µg of GnRH + 10
mg Domperidon]
— Ready to inject product
liquid](https://image.slidesharecdn.com/5b903481-7af9-43b1-9a62-0e92974b4b4a-160622065231/85/NSS-2016-ppt-3-320.jpg)
NSS 2016 ppt
- 1. Induced breeding of Tinfoil Barb (Barbonymus schwanenfeldii) (Bleeker, 1854) using Ovaprim . By E.D.M. Epasinghe, A.M.A.N. Adikari, T.A.D.W. Karunaratne, H.M.P. Kithsiri, V. Pahalawattarachchi
- 2. Tinfoil Barb [TFB] § Globally popular aquarium Cyprinid § Native to Southeast Asia § No authentic records on its natural spawning in captive condition in Sri Lanka Objective of breeding TFB § Inducing the spawning of captive reared TFB using Ovaprim § Introducing less time consuming and fruitful breeding method for commercial scale fish breeders in Sri Lanka
- 3. Materials and methods Inducing agent - Ovaprim — Uses to induce ovulation and spermiation in fish — A synthetic GnRH [1 mL contains 20 µg of GnRH + 10 mg Domperidon] — Ready to inject product liquid
- 4. Selection of brood fish — Selected randomly based on their external features. — Distended abdominal region- — Normal abdominal region- — Sedated fish in 65 mg/L TMS — Females were subjected to Intra Ovarian Biopsy. — Males were checked for secretion of milt Materials and methods
- 5. Confirmation of fish maturity, — 18 females with Migratory stage GV — 18 males Trickled milt when a slight pressure was applied at the vent Administration of Ovaprim within minimum possible period of time — Injected single dose — IM injection to the dorsal musculature Materials and methods Avg: BW 0.23 ± 0.01 kg
- 7. Dose mL/kg of BW 0.20 0.30 0.40 0.50 0.60 Control Un injected 0.10 0.15 0.20 0.25 0.30 Un injected The injected TFBs were kept at 100 L glass tank with preconditioned water aerating water continuously Sex ration 1:1 male to female Each experimental group comprised of three replicates. Materials and methods Hormone doses Breeding performance Ovulation time (hrs) Fertility rate (%) In addition, data collected on Post experimental mortality rate of brooders Water quality parameters in the experimental tanks
- 8. Identification of Ovulation time — In order to identify the correct ovulating time - after 3 hrs by the injection — Females vent was slightly pressed very carefully to facilitate come out eggs- repeated in every half an hour — Did not try by force — Expected free flow of eggs after completion of ovulation — Collecting of milt — Just after confirmed the complete ovulation in females — Males were stripped first — Fertilization — Dry method was followed to fertilize eggs — Fertility rate — Sub sample of eggs checked under the microscope to identify the first cleavage stage of embryonic development.
- 9. Cleavage stages of embryonic development of TFB 4 Cell 16 Cell Cleavage Blastula
- 10. Results and discussion. Dose mL/kg of BW 0.20 0.30 0.40 0.50 0.60 Control Un injected 0.10 0.15 0.20 0.25 0.30 Ovulation time/ hrs 11.00 ± 0.51a 9.20 ± 0.17b 5.43 ± 0.51c 6.10 ± 0.17c 3.20 ± 0.17d not observed Fertility rate % 27.84 ± 2.36a 50.5 ± 7.09b 73.33 ± 4.51c 80.67 ± 5.13c 33.33 ± 9.07a not observed Data was analyzed with one way ANOVA and the Tukey test in SPSS software. Values are presented as means ± S.D. Means in each raw with different superscripts are significantly different from each other.
- 11. 11.00 9.20 5.43 6.10 3.20 0.00 2.00 4.00 6.00 8.00 10.00 12.00 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 Ovulationtime(hrs) Dose- mL/kg of BW Relationship of Hormone dose Vs Ovulation time Dosage/mLkg-1 of BW 0.20 0.30 0.40 0.50 0.60 P value Ovulation time/ hrs 11.00 ± 0.51a 9.20 ± 0.17b 5.43 ± 0.51c 6.10 ± 0.17c 3.20 ± 0.17d 0.000 Fertility rate % 27.84 ± 2.36a 50.5 ± 7.09b 73.33 ± 4.51c 80.67 ± 5.13c 33.33 ± 9.07a 0.000
- 12. 27.84 50.50 73.33 80.67 33.33 0.00 10.00 20.00 30.00 40.00 50.00 60.00 70.00 80.00 90.00 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 Fertilityrate(%) Dose- mL/kg of BW Relationship of Hormone dose Vs Fertility rate Dosage/mLkg-1 of BW 0.20 0.30 0.40 0.50 0.60 P value Ovulation time/ hrs 11.00 ± 0.51a 9.20 ± 0.17b 5.43 ± 0.51c 6.10 ± 0.17c 3.20 ± 0.17d 0.000 Fertility rate % 27.84 ± 2.36a 50.5 ± 7.09b 73.33 ± 4.51c 80.67 ± 5.13c 33.33 ± 9.07a 0.000
- 13. Results and discussion. — In the post spawning period — survival rate of brooders was 100% — the range of hormone dosages - not harmful — During the latency period temperature ranged from 27.4 0C 27.8 0C , pH was measured as 7.3, Breeding tanks were aerated continuosly
- 14. Conclusion — Recommended dosages of obtain highest fertility rates and relatively short ovulation times are 0.4 -0.5 mlkg-1 single dosage of Ovaprim to the female and half the dosage to the male at a same time. Female fish should be induced when they are at the peak of GV migratory stage (stage IV) and, Male should ooze sperm with gentle pressure.
- 15. Dose calculation — This is a 1.0 mL cyringe. — It has been graduated in to the 50 units. — Therefore, a one unit is equal to 0.02 mL — We need to adjust 1 unit = 0.005 mL of hormone Normal saline 0.75 ml 0.25 ml hormone Dose mL/kg of BW 0.2 0.3 0.4 0.5 Recommended dose by producer 0.6 Control Un injected 0.1 0.15 0.2 0.25 0.3 Un injected For 0.23 kgFor 0.23 kg of BW /unitof BW /unit 9 14 18 23 28 4.5 7 9 12 14
- 16. To improve hatchability — Introduce different hatching techniques in order to increase the hatching rate — Eggs are semi buoyant Future research needs Effect of other hormone products which contains GnRH on TFB breeding should be monitored WOVA.FH , Ovatide®, Ovulin® - synthetic products