This document discusses methods for determining the concentration of chlorophyll in plant leaves. It describes how to extract chlorophyll from leaves using organic solvents like methanol or acetone. The concentration of chlorophyll is then determined using spectrophotometry by measuring absorption of light at wavelengths of 430, 645, and 663 nanometers. Calculations are provided to determine chlorophyll concentrations based on absorption measurements and sample properties. Equipment and supplies needed for chlorophyll extraction and analysis are also listed.