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Photosynthesis slides

 The process that occurs in green plants, whereby
solar energy is converted into chemical energy
and stored as organic molecules by making use
of carbon dioxide, sunlight, and water. Water
and Oxygen are formed as byproducts
 Photosynthesis can be summarized in the
following equation:
 6 CO2 + 12 H2O + Light
energy

 C6H12O6 + 6 O2 + 6
H2O
 (glucose)
WHAT IS PHOTOSYNTHESIS?

Photoautotrophs can photosynthesize.
Include: Green plants, algae, cyanobacteria
and green protists.
WHO OR WHAT CAN PHOTOSYNTHESIZE?
Plants
Green protists
Cyanobacteria
Algae

To provide nutrients and oxygen for
heterotrophs.
Heterotrophs are dependent on
autotrophs, because they cannot produce
there own food.
WHY DO PLANTS PHOTOSYNTHESIZE?
 Photosynthesis occurs in the chloroplasts of
plant cells.
 The chloroplasts are mainly concentrated in
the mesophyll cells of leaves.
 Chloroplast contain chlorophyll – green
pigment that absorbs sunlight.
 Chlorophyll fill the space in the thylakoid
membrane.
WHAT PART OF THE PLANT IS
RESPONSIBLE FOR PHOTOSYNTHESIS?
CHLOROPLAST MESOPHYLL CELL
(PALLISADE AND
SPONGY)
1 µm
Thylakoid
space
Chloroplast
Granum
Intermembrane
space
Inner
membrane
Outer
membrane
Stroma
Thylakoid
RAW MATERIALS OF
PHOTOSYNTHESIS
• The raw materials of
photosynthesis are:
 water,
carbon dioxide and
sunlight.
HOW RAW MATERIALS REACH
THE CHLOROPLASTS
 Water is absorbed through the root hair into the
xylem of the roots, into the xylem of the stem,
through the xylem of the leaves into the mesophyll
cells and finally into the chloroplasts.
 Carbon dioxide diffuses from the atmosphere
through the stomata, into the intercellular airspaces
in the leaves, and finally into the chloroplasts of the
mesophyll cells.
 The chlorophyll and other pigments in the
thylakoid membrane absorb the solar energy to
drive photosynthesis

LIGHT REACTION PHASE
(Dependent on light)
DARK PHASE/ CALVIN CYCLE
(Light independent)
PHOTOSYNTHESIS CONSIST OF 2
STAGES:

Takes place in the thylakoids of the chloroplasts.
Chlorophyll absorbs solar energy from the sun.
When a chlorophyll pigment absorbs light
(photon of energy), it excites the electrons, which
goes from ground state to an excited state, which
is unstable, but can be used as potential energy.
When unused excited e- fall back to the ground
state, photons and heat are given off.
LIGHT REACTION PHASE

 The electrons are excited in the
photosystems fount in the thylakoid
membrane.
This potential energy is then used firstly to
split water – into hydrogen & oxygen.
 2H2O 2H2 + O2
 Oxygen is released as a byproduct – diffuse
through stomata into atmosphere.
The hydrogen reduces NADP+ to NADPH
Some energy is then used to
photophosphorylate ADP to generate ATP.
 ADP + P ATP

Light
Fig. 10-5-2
H2O
Chloroplast
Light
Reactions
NADP+
P
ADP
i
+
ATP
NADPH
O2

 Carbon dioxide diffuses through the
stomata of the leave and finally into the
stroma of the chloroplast.
 The carbon dioxide is accepted by a 5C
molecule called ribulose biphosphate
(RuBP) which then forms an unstable 6C
compound.
 6C compound dissociates into 2 x 3C
compounds called phosphoglycerate
(PGA)
CALVIN CYCLE

 PGA is then reduced to phosphoglyceraldehyde
(PGAL/ G3P) by accepting a phosphate from ATP
and a hydrogen electron from NADPH.
Thus changing ATP back to ADP and NADPH to
NADP.
 PGAL are now used for the following reactions:
 Some PGAL are used to make RuBP again, so
that the cycle can start over again.
 Some PGAL are used to form hexose sugars like
glucose and fructose. Which combine to form
disaccharides and polysaccharides.
* The carbohydrates can then be converted to
other biological compounds like proteins or fats
by adding mineral salts like nitrates and
phoshates.

 CO2 +
 RuBP(5C)

 6C
compound


2x PGA (3C)

 PGAL
ATP = ADP + P
NADPH = NADP + H

.
Fig. 10-21
Light
Reactions:
Photosystem II
Electron transport chain
Photosystem I
Electron transport chain
CO2
NADP+
ADP
Pi
+
RuBP 3-Phosphoglycerate
Calvin
Cycle
G3PATP
NADPH
Starch
(storage)
Sucrose (export)
Chloroplast
Light
H2O
O2

 Light is a form of energy =
ELECTROMAGNETIC ENERGY/
ELECTROMAGNETIC RADIATION
 The electromagnetic energy travel in waves.
 Distance between crests of electromagnetic
waves = WAVELENGTH
 Wavelength range from ≤ 1nm (gamma rays) –
 ≥ 1 km (radio waves)
 The entire range of radiation wavelengths =
ELECTROMAGNETIC SPECTRUM
THE NATURE OF SUNLIGHT

ELECTROMAGNETIC SPECTRUM

UV
Fig. 10-6
Visible light
Infrared
Micro-
waves
Radio
wavesX-raysGamma
rays
103 m
1 m
(109 nm)106 nm103 nm1 nm10–3 nm10–5 nm
380 450 500 550 600 650 700 750 nm
Longer wavelength
Lower energyHigher energy
Shorter wavelength

The most important part for life is the visible
light (380nm – 750nm)
We can see this light as various colours.
Light consist of particles = PHOTONS
Photons have energy- The shorter the wave
length the greater the energy of the photon.
Therefore violet light has more energy than red
light.
Photosynthesis are driven by visible light of the
sun.

 Chlorophyll a – Absorb violet, blue and red light.
Reflects and transmits green light (that is why plant
leaves appear green)
 Chlorophyll b – Absorb violet, blue and red light.
Reflects and transmits green light (that is why plant
leaves appear green).
 Carotenoids – Play an accessory role in
photosynthesis. They are shades of yellow and
orange and able to absorb light in the violet-blue-
green range. These pigments become noticeable in
the fall when chlorophyll breaks down.

MAIN PIGMENTS USED DURING
PHOTOSYNTHESIS:
 The thylakoid membrane of a chroloplast
contains several photosystems.
 A photosystem consist of a protein complex
called a reaction-centre complex surrounded by
several light harvesting complexes.
 Study the diagram to understand the process of
light harvesting.
HOW A PHOTOSYSTEM HARVESTS
LIGHT

THYLAKOID SPACE
(INTERIOR OF THYLAKOID)
STROMA
e–
Pigment
molecules
Photon
Transfer
of energy
Special pair of
chlorophyll a
molecules
Thylakoidmembrane
Photosystem
Primary
electron
acceptor
Reaction-center
complex
Light-harvesting
complexes

The Importance of Photosynthesis: A Review
Energy entering chloroplasts as sunlight gets
stored as chemical energy in organic compounds
Sugar made in the chloroplasts supplies chemical
energy and carbon skeletons to synthesize the
organic molecules of cells.
Plants store excess sugar as starch in structures
such as roots, tubers, seeds, and fruits
In addition to food production, photosynthesis
produces the O2 in our atmosphere

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Photosynthesis slides

  • 2.   The process that occurs in green plants, whereby solar energy is converted into chemical energy and stored as organic molecules by making use of carbon dioxide, sunlight, and water. Water and Oxygen are formed as byproducts  Photosynthesis can be summarized in the following equation:  6 CO2 + 12 H2O + Light energy   C6H12O6 + 6 O2 + 6 H2O  (glucose) WHAT IS PHOTOSYNTHESIS?
  • 3.  Photoautotrophs can photosynthesize. Include: Green plants, algae, cyanobacteria and green protists. WHO OR WHAT CAN PHOTOSYNTHESIZE? Plants Green protists Cyanobacteria Algae
  • 4.  To provide nutrients and oxygen for heterotrophs. Heterotrophs are dependent on autotrophs, because they cannot produce there own food. WHY DO PLANTS PHOTOSYNTHESIZE?
  • 5.  Photosynthesis occurs in the chloroplasts of plant cells.  The chloroplasts are mainly concentrated in the mesophyll cells of leaves.  Chloroplast contain chlorophyll – green pigment that absorbs sunlight.  Chlorophyll fill the space in the thylakoid membrane. WHAT PART OF THE PLANT IS RESPONSIBLE FOR PHOTOSYNTHESIS?
  • 8. RAW MATERIALS OF PHOTOSYNTHESIS • The raw materials of photosynthesis are:  water, carbon dioxide and sunlight.
  • 9. HOW RAW MATERIALS REACH THE CHLOROPLASTS  Water is absorbed through the root hair into the xylem of the roots, into the xylem of the stem, through the xylem of the leaves into the mesophyll cells and finally into the chloroplasts.  Carbon dioxide diffuses from the atmosphere through the stomata, into the intercellular airspaces in the leaves, and finally into the chloroplasts of the mesophyll cells.  The chlorophyll and other pigments in the thylakoid membrane absorb the solar energy to drive photosynthesis
  • 10.  LIGHT REACTION PHASE (Dependent on light) DARK PHASE/ CALVIN CYCLE (Light independent) PHOTOSYNTHESIS CONSIST OF 2 STAGES:
  • 11.  Takes place in the thylakoids of the chloroplasts. Chlorophyll absorbs solar energy from the sun. When a chlorophyll pigment absorbs light (photon of energy), it excites the electrons, which goes from ground state to an excited state, which is unstable, but can be used as potential energy. When unused excited e- fall back to the ground state, photons and heat are given off. LIGHT REACTION PHASE
  • 12.   The electrons are excited in the photosystems fount in the thylakoid membrane. This potential energy is then used firstly to split water – into hydrogen & oxygen.  2H2O 2H2 + O2  Oxygen is released as a byproduct – diffuse through stomata into atmosphere. The hydrogen reduces NADP+ to NADPH Some energy is then used to photophosphorylate ADP to generate ATP.  ADP + P ATP
  • 14.   Carbon dioxide diffuses through the stomata of the leave and finally into the stroma of the chloroplast.  The carbon dioxide is accepted by a 5C molecule called ribulose biphosphate (RuBP) which then forms an unstable 6C compound.  6C compound dissociates into 2 x 3C compounds called phosphoglycerate (PGA) CALVIN CYCLE
  • 15.   PGA is then reduced to phosphoglyceraldehyde (PGAL/ G3P) by accepting a phosphate from ATP and a hydrogen electron from NADPH. Thus changing ATP back to ADP and NADPH to NADP.  PGAL are now used for the following reactions:  Some PGAL are used to make RuBP again, so that the cycle can start over again.  Some PGAL are used to form hexose sugars like glucose and fructose. Which combine to form disaccharides and polysaccharides. * The carbohydrates can then be converted to other biological compounds like proteins or fats by adding mineral salts like nitrates and phoshates.
  • 16.   CO2 +  RuBP(5C)   6C compound   2x PGA (3C)   PGAL ATP = ADP + P NADPH = NADP + H
  • 17.  . Fig. 10-21 Light Reactions: Photosystem II Electron transport chain Photosystem I Electron transport chain CO2 NADP+ ADP Pi + RuBP 3-Phosphoglycerate Calvin Cycle G3PATP NADPH Starch (storage) Sucrose (export) Chloroplast Light H2O O2
  • 18.   Light is a form of energy = ELECTROMAGNETIC ENERGY/ ELECTROMAGNETIC RADIATION  The electromagnetic energy travel in waves.  Distance between crests of electromagnetic waves = WAVELENGTH  Wavelength range from ≤ 1nm (gamma rays) –  ≥ 1 km (radio waves)  The entire range of radiation wavelengths = ELECTROMAGNETIC SPECTRUM THE NATURE OF SUNLIGHT
  • 20.  UV Fig. 10-6 Visible light Infrared Micro- waves Radio wavesX-raysGamma rays 103 m 1 m (109 nm)106 nm103 nm1 nm10–3 nm10–5 nm 380 450 500 550 600 650 700 750 nm Longer wavelength Lower energyHigher energy Shorter wavelength
  • 21.  The most important part for life is the visible light (380nm – 750nm) We can see this light as various colours. Light consist of particles = PHOTONS Photons have energy- The shorter the wave length the greater the energy of the photon. Therefore violet light has more energy than red light. Photosynthesis are driven by visible light of the sun.
  • 22.   Chlorophyll a – Absorb violet, blue and red light. Reflects and transmits green light (that is why plant leaves appear green)  Chlorophyll b – Absorb violet, blue and red light. Reflects and transmits green light (that is why plant leaves appear green).  Carotenoids – Play an accessory role in photosynthesis. They are shades of yellow and orange and able to absorb light in the violet-blue- green range. These pigments become noticeable in the fall when chlorophyll breaks down.  MAIN PIGMENTS USED DURING PHOTOSYNTHESIS:
  • 23.  The thylakoid membrane of a chroloplast contains several photosystems.  A photosystem consist of a protein complex called a reaction-centre complex surrounded by several light harvesting complexes.  Study the diagram to understand the process of light harvesting. HOW A PHOTOSYSTEM HARVESTS LIGHT
  • 24.  THYLAKOID SPACE (INTERIOR OF THYLAKOID) STROMA e– Pigment molecules Photon Transfer of energy Special pair of chlorophyll a molecules Thylakoidmembrane Photosystem Primary electron acceptor Reaction-center complex Light-harvesting complexes
  • 25.  The Importance of Photosynthesis: A Review Energy entering chloroplasts as sunlight gets stored as chemical energy in organic compounds Sugar made in the chloroplasts supplies chemical energy and carbon skeletons to synthesize the organic molecules of cells. Plants store excess sugar as starch in structures such as roots, tubers, seeds, and fruits In addition to food production, photosynthesis produces the O2 in our atmosphere