plasmid isolation.HARIS
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This document discusses the three main steps to isolate plasmid DNA from bacterial cells: 1) Cell lysis using alkaline lysis with SDS to break open the cells and release the plasmid DNA, 2) Deproteinization and removal of chromosomal DNA, and 3) Precipitation of the purified plasmid DNA. The isolation of plasmid DNA is important for studying extrachromosomal DNA molecules that are capable of autonomous replication in bacteria and yeast.
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plasmid isolation.HARIS
- 1. HARIS P MSC BIOTECHNOLOGY M.A.COLLEGE KOTHAMANGALAM ISOLATION OF PLASMID DNA BY ALKALINE LYSIS WITH SDS
- 2. Plasmid is an extra chromosomal DNA molecule which is capable of self replication. It is circular and double stranded. Plasmids naturally occur in bacteria but are also found in eukaryotic yeast.
- 3. HOW PLASMIDS ARE ISOLATED??? 3 MAIN STEPS:- CELL LYSIS DEPROTEINATION & REMOVAL OF Chr.DNA PRECIPITATION OF PLASMID DNA