Post harvest diseases of citrus
Dr. P. Kishore Varma
Assistant professor
PJTSAU, Hyderabad
Green and blue mold – Penicillium spp.
Green mold
• Penicillium digitatum
(green mould)
• P. italicum (blue mould)
• Softening of damaged
tissue.
• White fungal growth,
which progressively turns
blue or green as spores
develop.
• Infections develop
from damaged areas.
• The growth of mould
increases with storage
• Optimum temperatures
(20-270
C).
• Late season fruit more
susceptible.
• Damaged rind is more
susceptible.
3
Blue mold
Green and blue mold – Penicillium spp.
Pathogen biology – Penicillium digitatum
Penicillium digitatum
Penicillium italicum
Sour rot - Geotrichum candidum / G. citri-aurantii
• Very soft, watery decay.
• Distinct margin between
decayed and healthy tissue.
• Lesions covered with slimy
off-white spores
• Sour odour detectable.
• Prevalent after warm
wet areas
• Pathogenic on ‘weak’,
wounded, bruised, and
split fruit
• Lemons, limes and
grape fruits stored for
extended periods
• Ripe fruit is more
susceptible
6
7
Geotrichum candidum
8
Stem end rot - Lasiodiplodia theobromae
• Softening of tissue
around button
• Brown rind with dark
flesh beneath
• Rapid decay down the
axis of the fruit
reaching stylar end
• Abundant rainfall during
crops eason
• Ethylene degreening
treatments predispose fruit to
Botryodiplodia infection
• Quiesecent in floral remnants
under the sepals of fruit
• Botryodiplodia – 300
C
• Phomopsis, Dothiorella -
230
C
9
10
Botryodiplodia theobromae
Phomopsis citri
Dothiorella
13
Brown rot - Phytophthora citrophthora
• Greyish brown firm and
leathery spot
• Pungent odour
• Fine white spore bearing
mould on peel
• Develops rapidly at - 250
C
Phytophthora citrophthora, P. citricola, P. hibernalis
14
Anthracnose - Colletotrichum gloeosporioides
Tear stain
• Superficial leathery
appearance
• Silver/grey to dark lesions.
• Tear-staining pattern
• Pink tinge (spores) under
humid conditions.
• Infection occurs by
rain-splash during
autumn.
• ethylene degreening
increases sensitivity to
anthracnose
• Quiescent - appressoria
16
Colletotrichum gloeosporioides
17
Trichoderma rot - Trichoderma viride
• Peel turns dark brown
• Veins on the interior
surface of the peel turn
brown
• Odour of coconuts
• Yellow green to dark
green sporulation on
surface
Trichoderma viride
19
Management
• Careful handling
• Sanitation
• Sodium-ortho phenyl phenate, sodium carbonate
• Benzimidazole and imazalil
• Curing citrus fruits at 360
C for 3 days
• Yeast, eg. Debaryomyces, Pichia
• Candida famata, Pseudomonas syringae,
Aureobasidium pullulans
• Aspire – Candida oleophila
• Biosave – P. syringae
20
Management
• Stem end rots – TBZ, Benomyl, Imazalil,
prochloraz
• Brown rot – Fixed copper fungicides, Metalaxyl,
Fosetyl-Al
• Hot water dip treatments at 46-490
C for 3 min
• Stem-end rots – Refrigeration between 4-70
C
• Anthracnose- <100
C
21
Citrus disorders
Chilling injury
• Critical temperature – 10-150
C for grapefruit,
lemons and lime
• 1 and 50
C – Oranges, mandarins
• Brown pitting of rind
• Watery breakdown
• Brown staining of peel or peel tissue collapse
• Oil gland darkening – grapefruits
• Albedo browning, membranous stain - lemons
22
23
Chilling injury
• Abnormal biochemical changes
• Moisture loss from peel
• Growth regulators and fungicides
• Varies from season to season
Chilling injury vs. Pitting
24
Peel tissue collapse
Develops in 4-6 weeks in storage
Reduced by waxing
Oil gland targeted
Develops in 3-4 days
Caused by wax application
Chilling injury-Management
• Reduction of moisture loss by applying oil, wax
(shellac) or film
• Maintain high humidity
• Sealing in polyethylene film
• Pre-conditioning of fruits to chilling temperatures
• Intermittent heating
• Fungicide application (eg. TBZ)
• Low oxygen and high CO2
25
Optimal temperature with proper wax is the best means for
controlling chilling injury
Freezing injury
• Temperature below -1.50
C
• Browning of peel
• Water soaked membranes between the
segments
• Mandarins- crystals in pulp
• Grapefruit – milky appearance of pulp
• Cavities in flesh
26
27
Oleocellosis or rind-oil spotting
• Common on lemons and limes
• Injured areas firm, irregular in shape
• Yellow, green or brown discolouration
• Oil glands appear prominent
Reasons
• Compression of fruits
• Sudden spell of cold weather
• Physical injury to the peel
• Fruits harvested in cool, wet periods
28
Oleocellosis
Management
• Pre-harvest spray of ethylene
• Avoid harvesting immature turgid fruits
• Avoid picking too early in the season
29
Other disorders
30
Creasing Chemical injury
Peteca Puffiness

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Post harvest lecture citrus-1

  • 1. Post harvest diseases of citrus Dr. P. Kishore Varma Assistant professor PJTSAU, Hyderabad
  • 2. Green and blue mold – Penicillium spp. Green mold • Penicillium digitatum (green mould) • P. italicum (blue mould) • Softening of damaged tissue. • White fungal growth, which progressively turns blue or green as spores develop.
  • 3. • Infections develop from damaged areas. • The growth of mould increases with storage • Optimum temperatures (20-270 C). • Late season fruit more susceptible. • Damaged rind is more susceptible. 3 Blue mold Green and blue mold – Penicillium spp.
  • 4. Pathogen biology – Penicillium digitatum Penicillium digitatum Penicillium italicum
  • 5. Sour rot - Geotrichum candidum / G. citri-aurantii • Very soft, watery decay. • Distinct margin between decayed and healthy tissue. • Lesions covered with slimy off-white spores • Sour odour detectable.
  • 6. • Prevalent after warm wet areas • Pathogenic on ‘weak’, wounded, bruised, and split fruit • Lemons, limes and grape fruits stored for extended periods • Ripe fruit is more susceptible 6
  • 8. 8 Stem end rot - Lasiodiplodia theobromae • Softening of tissue around button • Brown rind with dark flesh beneath • Rapid decay down the axis of the fruit reaching stylar end
  • 9. • Abundant rainfall during crops eason • Ethylene degreening treatments predispose fruit to Botryodiplodia infection • Quiesecent in floral remnants under the sepals of fruit • Botryodiplodia – 300 C • Phomopsis, Dothiorella - 230 C 9
  • 13. 13 Brown rot - Phytophthora citrophthora • Greyish brown firm and leathery spot • Pungent odour • Fine white spore bearing mould on peel • Develops rapidly at - 250 C
  • 14. Phytophthora citrophthora, P. citricola, P. hibernalis 14
  • 15. Anthracnose - Colletotrichum gloeosporioides Tear stain • Superficial leathery appearance • Silver/grey to dark lesions. • Tear-staining pattern • Pink tinge (spores) under humid conditions.
  • 16. • Infection occurs by rain-splash during autumn. • ethylene degreening increases sensitivity to anthracnose • Quiescent - appressoria 16
  • 18. Trichoderma rot - Trichoderma viride • Peel turns dark brown • Veins on the interior surface of the peel turn brown • Odour of coconuts • Yellow green to dark green sporulation on surface
  • 20. Management • Careful handling • Sanitation • Sodium-ortho phenyl phenate, sodium carbonate • Benzimidazole and imazalil • Curing citrus fruits at 360 C for 3 days • Yeast, eg. Debaryomyces, Pichia • Candida famata, Pseudomonas syringae, Aureobasidium pullulans • Aspire – Candida oleophila • Biosave – P. syringae 20
  • 21. Management • Stem end rots – TBZ, Benomyl, Imazalil, prochloraz • Brown rot – Fixed copper fungicides, Metalaxyl, Fosetyl-Al • Hot water dip treatments at 46-490 C for 3 min • Stem-end rots – Refrigeration between 4-70 C • Anthracnose- <100 C 21
  • 22. Citrus disorders Chilling injury • Critical temperature – 10-150 C for grapefruit, lemons and lime • 1 and 50 C – Oranges, mandarins • Brown pitting of rind • Watery breakdown • Brown staining of peel or peel tissue collapse • Oil gland darkening – grapefruits • Albedo browning, membranous stain - lemons 22
  • 23. 23
  • 24. Chilling injury • Abnormal biochemical changes • Moisture loss from peel • Growth regulators and fungicides • Varies from season to season Chilling injury vs. Pitting 24 Peel tissue collapse Develops in 4-6 weeks in storage Reduced by waxing Oil gland targeted Develops in 3-4 days Caused by wax application
  • 25. Chilling injury-Management • Reduction of moisture loss by applying oil, wax (shellac) or film • Maintain high humidity • Sealing in polyethylene film • Pre-conditioning of fruits to chilling temperatures • Intermittent heating • Fungicide application (eg. TBZ) • Low oxygen and high CO2 25 Optimal temperature with proper wax is the best means for controlling chilling injury
  • 26. Freezing injury • Temperature below -1.50 C • Browning of peel • Water soaked membranes between the segments • Mandarins- crystals in pulp • Grapefruit – milky appearance of pulp • Cavities in flesh 26
  • 27. 27
  • 28. Oleocellosis or rind-oil spotting • Common on lemons and limes • Injured areas firm, irregular in shape • Yellow, green or brown discolouration • Oil glands appear prominent Reasons • Compression of fruits • Sudden spell of cold weather • Physical injury to the peel • Fruits harvested in cool, wet periods 28
  • 29. Oleocellosis Management • Pre-harvest spray of ethylene • Avoid harvesting immature turgid fruits • Avoid picking too early in the season 29
  • 30. Other disorders 30 Creasing Chemical injury Peteca Puffiness