Ppt Presentation on sterilization methods
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The document discusses various sterilization methods, categorized into physical, chemical, mechanical, and radiation methods. It emphasizes the importance of sterilization in preventing contamination, spoilage, and the transmission of pathogens. Key sterilization techniques include dry and moist heat, gaseous agents, filtration, and radiation methods.
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Ppt Presentation on sterilization methods
- 1. Microbiology Sterilization methods Ms. Ayodhya Pardhe (M Pharmacy) Sudhakar Rao Naik institute of pharmacy Pusad
- 2. Contents • Introduction • Importance of sterilization • Sterilization methods 1. Physical methods 2. Chemical methods 3. Mechanical methods 4. Radiation methods
- 5. Importance of sterilization • To prevent contamination in sterile products • To prevent decomposition and spoilage of food products • To prevent transmission of pathogenic microorganisms which responsible for causing diseases. • To prevent contamination of unwanted microbes in pure culture media • To prevent unwanted microbes contamination in antibiotics, enzymes, vitamins etc • To prevent contamination in aseptic areas which are used for the preparation of sterile dosage form and sterility testing
- 6. Methods of sterilization 1. Physical methods • Dry heat sterilization eg. Hot air oven, red heat, direct flame, incineration • Moist heat sterilization eg. Autoclave, pasturization, tyndallization 2. Chemical methods • Gaseous sterilization eg. Formaldehyde, ethylene oxide etc • By using disinfectants eg. Cresol, phenol etc
- 7. 3. Mechanical methods ( filtration methods) • Asbestos filter (Seitz filter) • Filter candles (ceramic or Berkefeld filter) • Membrane filter • Sintered glass filter ( morton filter) 4. Radiation methods • Use of ultra violet rays: UV rays • Ionizing radiation: X-rays, gamma rays, beta rays
- 40. Thank you!