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Science Further Ahead:
NGS
Archana Kulkarni
RASA Life Sciences
Development of NGS
 The growing power and reducing cost sparked an
enormous range of applications of Next generation
sequencing (NGS) technology.
 The first NGS platform, was developed and put on
the market by Roche in 2005.
 The development of the next-generation sequencing
(NGS) technologies has contributed to this trend
substantially by reducing costs and producing
massive sequencing data.
Development of NGS
Working of Illumina and Ion Torrent Sequencing
Working of Illumina and Ion Torrent Sequencing
Working of Illumina and Ion Torrent Sequencing
Template generation via Bridge Amplification (A) or emPCR (B).
Instrument Comparison
Instruments may differ at Three Levels:
1. Read Alignments
2. Statistical Analysis
3. Final Biological Interferences
Comparison of Working
Advantages and Disadvantages
Thank You

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Presentation of NGS ppt