Recombinant DNA, probes and Techniques
- 1. Cindy Vargas Forero Third Semester Medicine – UPB 2010
- 4. Recombinant DNA, involves creating VOLUNTARILY DNA molecules consisting of DNA fragments coming from different sources probes are short fragments of nucleic acids are used for identification of gens
- 6. Also improved their ability to detect smaller concentrations of reactive oxygen species. Replacing a hydrogen atom with a deuterium Increased the stability and shelf life of the dyes,
- 7. Deuterium is an isotope of hydrogen that has a single proton and single neutron in its nucleus. Its atomic weight is therefore twice that of the much more common hydrogen atom, which lacks a neutron.
- 8. When they designed and synthesized various fluorescent probes with deuterium instead of hydrogen the dyes were not as susceptible to spontaneous degradation by air and light as their hydrogen counterparts which made them significantly more accurate at detecting reactive oxygen species in cells and animals.
- 9. It is important to understan that reactive oxygen species are the produc of cellular stress and reacts better on probes that have change a hydrogen in fluoresent dyes
- 11. Researchers from MBIC are turning up the brightness on a group of fluorescent probes called fluoromodules.
- 12. Fluoromodules that are used to monitor biological activities of individual proteins in real-time.
- 13. This latest advance enhances their fluormodule technology by causing it to glow an order of magnitude brighter than typical fluorescent proteins. The new fluoromodules are five- to seven-times brighter than enhanced green fluorescent protein (EGFP)
- 14. This flouromodules makes brighter proteis, because it amplified the signal, so it makes possible to follow an specific protein in real time .
- 16. Fluorescent probes are essential to studying a variety of inflammatory diseases, including cancer and atherosclerosis.
- 17. The probes detec and measure reactive oxygen species, which play an important role in disease processes The probes detect and measure reactive oxygen species, which play an important role in disease processes. The probes detect and measure reactive oxygen species, which play an important role in disease processes. The probes detect and measure reactive oxygen species, which play an important role in disease processes.
- 18. Fluoro modules, can be used to monitor biological activities of individual proteins in living cells in real time. nervous system diseases such as Parkinson's, autism, Alzheimer. the expression of genes and proteins in people with mutations, evaluate specific proteins of different types of cancer
- 19. MARTINEZ, S. Lina Maria. Biologia Molecular. Quinta edición. Medellin. UPB. Fac de Medicina, 2009. “ Replacing Hydrogen in Fluorescent Dyes Improves Detection Ability, Stability and Shelf Life”. Science Daily. July 20, 2010 “ Brightness on Fluorescent Probes Used to Monitor Biological Activities of Individual Proteins Increased ”. Science Daily. Aug. 19, 2010