Revision Scheme - Prac. Microbiology
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This document contains slides and information about microbiological testing and identification of various bacteria and fungi. It includes schemes for staining bacteria with and without background staining. It also includes images of various microbes growing on different media like blood agar, chocolate agar and Loffler's serum. Various tests are described like coagulase testing of Staphylococcus, antibiotic sensitivity testing, phage typing and serological testing like Elek's test and tube agglutination testing.
Revision Scheme - Prac. Microbiology
- 2. SLIDES
- 3. Schemes for slides With background Without background Gram positive cocci Gram positive bacilli Gram negative cocci
- 4. Scheme for slides With background Without background Brown Blue Red Organism Gram +ve cocci Organism Gram -ve
- 9. Candida
- 18. Vincent’s angina
- 19. Mycobacterium tuberculosis (Acid Fast bacilli Z-N stain)
- 20. Mycobacterium tuberculosis (Acid Fast bacilli Z-N stain)
- 21. Mycobacterium tuberculosis (Acid Fast bacilli Z-N stain)
- 25. MEDIA
- 26. Nutrient agar
- 27. Staphylococcus aureus on nutrient agar ( showing golden yellow endopigment )
- 28. Blood agar
- 29. Types of hemolysis on Blood agar
- 30. Alpha-hemolysis on blood agar
- 31. Beta-hemolysis on blood agar
- 32. Non-hemolysis on blood agar
- 34. Loffler’s serum for C. diphtheriae
- 35. Lowenstein Jensen medium for M. tuberculosis
- 36. Cooked meat medium for anaerobic cultivation
- 37. TESTS
- 38. Coagulase Test Staphylococcus aureus Coagulase negative staphylococci, e.g. S. epidermidis
- 39. Coagulase test Staphylococcus aureus Coagulase negative staphylococci, e.g. S. epidermidis
- 40. Antibiotic Sensitivity Test (Disc diffusion) Sensitive to: 1, 2, 3, 4, 6 Resistant to: 5 1 5 62 4 3
- 41. Antibiotic Sensitivity Test (Disc diffusion) Sensitive to: 1, 2, 5, 6 Resistant to: 3, 4 1 5 6 2 4 3
- 42. Phage typing 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 Sensitive to 4, 5, 6,……..etc.
- 43. SEROLOGY
- 44. Elek’s Test Toxigenic C. diphtheriae Non-toxigenic C. diphtheriae
- 45. Simple tube agglutination test (Titre:1/320) TITRE 1/20 1/40 1/80 1/160 1/320 1/640 1/1280 - ve control