RNA interference & its application
- 1. R N A I N T E R F E R E N C E A N D I T S A P P L I C AT I O N S B Y - A K R I T I G A U R A V
- 2. Overview 1. Introduction 2. Mechanism 3. Recent Application 4. Conclusion
- 3. Several terms are used to described RNA silencing; usually there are three phenotypically different but mechanistically similar phenomena: 1. Co-suppression or post-transcriptional gene silencing (PTGS) in plants 2. Quelling in fungi 3. RNA interference in animal kingdom Introduction
- 4. Definition RNA interference (RNAi) is a mechanism that inhibits gene expression at the stage of translation or by hindering the transcription of specific genes.
- 6. Recent applications of RNAi • Modulation of HIV-1 replication by RNA interference. • Potent and specific inhibition of human immunodeficiency virus type 1 replication by RNA interference. • In the treatment of Hepatitis. • It is used to intervene in the process of atherosclerosis or to reduce damage to heart tissue and brain cells.
- 7. • RNAi is a powerful and attractive genetic approach because of the diversity of its applications. The potential uses currently in progress include the identification of specific gene functions in living systems and creation of genome wide screens. Development of antiviral and anticancer therapies are broadening the horizons of the therapeutic arena. • Another value of RNAi screens is in combining it with other functional genomic assays enabling mapping of biochemical pathways. Impact of RNAi is also being extended to the field of agriculture for example by increasing disease resistance in plants. Conclusions