A Simulation Study of Segmentation Methods on the Soil Aggregate Microtomographic Images Wei Wang, Alexandra N. Kravchenko, Kateryna Ananyeva,  Alvin J. M. Smucker, C.Y. Lim and Mark L. Rivers   Department of Crop & Soil Sciences, Department of Statistics & Probability, MSU Advanced Photon Source, Argonne National Laboratory
Computed microtomographic images (CMT)
Motivation Segmentation results will affect pore network analysis, (e.g. pore connectivity, tortuosity, medial axis … ) and therefore influence flow simulation (e.g. Lattice Boltzmann modeling ), pore-scale biological activity modeling. Accurate pore/solid classification is very important to understand  pore structures in the intra-aggregate spaces.
Difficulties in processing CMT images Artifacts : partial volume effect (finite resolution effect), beam hardening, ring artifacts … Complex composition of soil matrix (large range of grey-scale values)
Difficulties in processing CMT images Lack of ground-truth information to assess pore/solid classification accuracy Identify criteria to select optimal segmentation method for soil aggregate images
Objectives To evaluate criteria for selecting the optimal segmentation method for soil pore characterization To compare performance of several commonly used segmentation methods in soil aggregate images with different porosities
Simulation approach In order to overcome the absence of ground-truth information we proposed a simulation approach including: ●  Simulate partial volume effect in the pore space ●  Simulate different solid material ●  Simulate   random noise
Simulate partial volume effect Generate binary soil images at the scanned resolution;  scanned pixel size Ground-truth  image 1 mm
Simulate partial volume effect Generate  3 layers of pores at smaller scales; 1/2 scanned pixel size 1 mm 1/8 scanned pixel size 1 mm 1/4 scanned pixel size 1 mm
Simulate partial volume effect Combine all the layers of pores 1 mm
Simulate solid space and noise Solid space simulation was done for all the  “ white ”  pixels  using spatial simulation of LU decomposition technique Gaussian random noise was added to the whole image
Grey scale image simulation Ground truth image Simulation in the pore space Simulation in the solid space + noise simulation Original image from the scan
Different porosity cases (1) Low Medium High High + flow pattern Porosity = 4.8% Porosity = 7.8% Porosity = 16.5% Porosity = 22.8%
Different porosity cases (2) Low Medium High High + flow pattern Porosity = 3.6% Porosity = 8.3% Porosity = 15.8% Porosity = 28.5%
Existing segmentation methods More than 40 different segmentation methods (Sezgin et al., 2004 ) They mainly can be classified into several categories:   ●   Manual thresholding ●   Global thresholding methods  ●   Local adaptive methods
Segmentation methods Global thresholding : ●   Entropy  method:  Renyi ’ s entropy (Sahoo et al., 1997)  ●   Iterative  method: Riddler et al., 1978  ●   Otsu  method: maximize between-class variance (Otsu, 1979) Local adaptive method: ●   Indicator kriging  ( IK ) method:  Oh and Lindquist, 1999
IK method Two steps : thresholding, kriging Thresholding step: the thresholds are determined by fitting mixed Gaussian distributions to pore and solid spaces using Expectation-Maximization algorithm (Dempster et al., 1977 ).  Black White T 1 T 2 Solid Pore kriging step ?
Segmentation performance criterion Misclassification Error (ME): 0<ME<1 (ground-truth image required) where  P  and  S  are the number of common pore or solid pixels in both ground-truth and segmented images.
Segmentation performance criterion Region non-uniformity measure (NU): 0<NU<1 (ground-truth image not required) Where  P  and T are the numbers of pore and total number of pixels in the segmented image,  and  are the variance of grey-scale values in the pore space and total variance in the simulated grayscale image.  Whether NU can be used as a criterion for soil  ?
How good is NU for soil ?   Pore morphological characteristics: Porosity Number of connected pores Number of pore boundary pixels Number of pore skeleton pixels
Results (Low porosity) Ground truth image IK Entropy Iterative Otsu Distinct segmentation error
Results (Medium porosity) Ground truth image IK Entropy Iterative Otsu
Results (High porosity) Ground truth image IK Entropy Iterative Otsu
Results (High+flow pattern) Ground truth image IK Entropy Iterative Otsu
Comparisons of segmentation methods using ME and NU Overall ranking by ME :  IK > Entropy > Iterative > Otsu  Overall ranking by NU :  IK > Otsu > Iterative > Entropy Indicator Kriging is the best! Indicator Kriging is the best! IK Iter Otsu Entropy Entropy IK Otsu Iter
How good is NU for preserving pore characteristics ? * Relative error = ( the pore characteristic value from the segmented image - the pore characteristic ground-truth value)/ the ground-truth value
Summary Soil aggregate CMT images were generated from the pore/solid binary image by simulating  partial volume effect, different solid material and background noise.  No single method preserved pore characteristics in all cases. However,  Indicator Kriging  method yielded segmented images most similar to the ground-truth images in the majority of cases studied.
Summary We recommend using NU as a criterion for choosing best segmentation approaches. Segmentation assessment using NU provides  acceptable representation of pore characteristics in the segmented images.
USDA-CSREES National Research Initiative:  Project 2008-35102-04567 NSF LTER Program at Kellogg Biological Station and the Michigan Agricultural Experiment Station Advanced Photon Source, Argonne National Lab Acknowledgement
Thanks for your attention!
References M. Sezgin, B. Sankur. 2004. Survey over image thresholding techniques and quantitative performance evaluation. Journal of Electronic Imaging 13(1), 146 – 165 P. Sahoo, C. Wilkins and J. Yeager. 1997. Threshold selection using Renyi ’ s entropy. Pattern Recognition, Vol.1, No.1, 71-84 W. Oh, B. Lindquist. 1999. Image thesholding by indicator kriging. IEEE Transactions on Pattern Analysis and Machine Intelligence 21: 590-602. T. W. Ridler and S. Calvard,  ‘‘ Picture thresholding using an iterative selection method, ’’  IEEE Trans. Syst. Man Cybern. SMC-8, 630 – 632 ~1978. N. Otsu (1979). &quot;A threshold selection method from gray-level histograms&quot;.  IEEE Trans. Sys., Man., Cyber.  9: 62 – 66 Dempster, A.P., Laird, N.M. and Rubin, D.B., 1977. Maximum likelihood from in- complete data via the em algorithm. Journal of the Royal Statistical Society: Series B, 39(1): 1-38.

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S S S A2009 Simulation Study Of Segmentation

  • 1. A Simulation Study of Segmentation Methods on the Soil Aggregate Microtomographic Images Wei Wang, Alexandra N. Kravchenko, Kateryna Ananyeva, Alvin J. M. Smucker, C.Y. Lim and Mark L. Rivers Department of Crop & Soil Sciences, Department of Statistics & Probability, MSU Advanced Photon Source, Argonne National Laboratory
  • 3. Motivation Segmentation results will affect pore network analysis, (e.g. pore connectivity, tortuosity, medial axis … ) and therefore influence flow simulation (e.g. Lattice Boltzmann modeling ), pore-scale biological activity modeling. Accurate pore/solid classification is very important to understand pore structures in the intra-aggregate spaces.
  • 4. Difficulties in processing CMT images Artifacts : partial volume effect (finite resolution effect), beam hardening, ring artifacts … Complex composition of soil matrix (large range of grey-scale values)
  • 5. Difficulties in processing CMT images Lack of ground-truth information to assess pore/solid classification accuracy Identify criteria to select optimal segmentation method for soil aggregate images
  • 6. Objectives To evaluate criteria for selecting the optimal segmentation method for soil pore characterization To compare performance of several commonly used segmentation methods in soil aggregate images with different porosities
  • 7. Simulation approach In order to overcome the absence of ground-truth information we proposed a simulation approach including: ● Simulate partial volume effect in the pore space ● Simulate different solid material ● Simulate random noise
  • 8. Simulate partial volume effect Generate binary soil images at the scanned resolution; scanned pixel size Ground-truth image 1 mm
  • 9. Simulate partial volume effect Generate 3 layers of pores at smaller scales; 1/2 scanned pixel size 1 mm 1/8 scanned pixel size 1 mm 1/4 scanned pixel size 1 mm
  • 10. Simulate partial volume effect Combine all the layers of pores 1 mm
  • 11. Simulate solid space and noise Solid space simulation was done for all the “ white ” pixels using spatial simulation of LU decomposition technique Gaussian random noise was added to the whole image
  • 12. Grey scale image simulation Ground truth image Simulation in the pore space Simulation in the solid space + noise simulation Original image from the scan
  • 13. Different porosity cases (1) Low Medium High High + flow pattern Porosity = 4.8% Porosity = 7.8% Porosity = 16.5% Porosity = 22.8%
  • 14. Different porosity cases (2) Low Medium High High + flow pattern Porosity = 3.6% Porosity = 8.3% Porosity = 15.8% Porosity = 28.5%
  • 15. Existing segmentation methods More than 40 different segmentation methods (Sezgin et al., 2004 ) They mainly can be classified into several categories: ● Manual thresholding ● Global thresholding methods ● Local adaptive methods
  • 16. Segmentation methods Global thresholding : ● Entropy method: Renyi ’ s entropy (Sahoo et al., 1997) ● Iterative method: Riddler et al., 1978 ● Otsu method: maximize between-class variance (Otsu, 1979) Local adaptive method: ● Indicator kriging ( IK ) method: Oh and Lindquist, 1999
  • 17. IK method Two steps : thresholding, kriging Thresholding step: the thresholds are determined by fitting mixed Gaussian distributions to pore and solid spaces using Expectation-Maximization algorithm (Dempster et al., 1977 ). Black White T 1 T 2 Solid Pore kriging step ?
  • 18. Segmentation performance criterion Misclassification Error (ME): 0<ME<1 (ground-truth image required) where P and S are the number of common pore or solid pixels in both ground-truth and segmented images.
  • 19. Segmentation performance criterion Region non-uniformity measure (NU): 0<NU<1 (ground-truth image not required) Where P and T are the numbers of pore and total number of pixels in the segmented image, and are the variance of grey-scale values in the pore space and total variance in the simulated grayscale image. Whether NU can be used as a criterion for soil ?
  • 20. How good is NU for soil ? Pore morphological characteristics: Porosity Number of connected pores Number of pore boundary pixels Number of pore skeleton pixels
  • 21. Results (Low porosity) Ground truth image IK Entropy Iterative Otsu Distinct segmentation error
  • 22. Results (Medium porosity) Ground truth image IK Entropy Iterative Otsu
  • 23. Results (High porosity) Ground truth image IK Entropy Iterative Otsu
  • 24. Results (High+flow pattern) Ground truth image IK Entropy Iterative Otsu
  • 25. Comparisons of segmentation methods using ME and NU Overall ranking by ME : IK > Entropy > Iterative > Otsu Overall ranking by NU : IK > Otsu > Iterative > Entropy Indicator Kriging is the best! Indicator Kriging is the best! IK Iter Otsu Entropy Entropy IK Otsu Iter
  • 26. How good is NU for preserving pore characteristics ? * Relative error = ( the pore characteristic value from the segmented image - the pore characteristic ground-truth value)/ the ground-truth value
  • 27. Summary Soil aggregate CMT images were generated from the pore/solid binary image by simulating partial volume effect, different solid material and background noise. No single method preserved pore characteristics in all cases. However, Indicator Kriging method yielded segmented images most similar to the ground-truth images in the majority of cases studied.
  • 28. Summary We recommend using NU as a criterion for choosing best segmentation approaches. Segmentation assessment using NU provides acceptable representation of pore characteristics in the segmented images.
  • 29. USDA-CSREES National Research Initiative: Project 2008-35102-04567 NSF LTER Program at Kellogg Biological Station and the Michigan Agricultural Experiment Station Advanced Photon Source, Argonne National Lab Acknowledgement
  • 30. Thanks for your attention!
  • 31. References M. Sezgin, B. Sankur. 2004. Survey over image thresholding techniques and quantitative performance evaluation. Journal of Electronic Imaging 13(1), 146 – 165 P. Sahoo, C. Wilkins and J. Yeager. 1997. Threshold selection using Renyi ’ s entropy. Pattern Recognition, Vol.1, No.1, 71-84 W. Oh, B. Lindquist. 1999. Image thesholding by indicator kriging. IEEE Transactions on Pattern Analysis and Machine Intelligence 21: 590-602. T. W. Ridler and S. Calvard, ‘‘ Picture thresholding using an iterative selection method, ’’ IEEE Trans. Syst. Man Cybern. SMC-8, 630 – 632 ~1978. N. Otsu (1979). &quot;A threshold selection method from gray-level histograms&quot;. IEEE Trans. Sys., Man., Cyber. 9: 62 – 66 Dempster, A.P., Laird, N.M. and Rubin, D.B., 1977. Maximum likelihood from in- complete data via the em algorithm. Journal of the Royal Statistical Society: Series B, 39(1): 1-38.