The process of forming a protein begins with transcription in the nucleus, where RNA polymerase binds to a promoter, synthesizing mRNA through complementary base pairing, replacing thymine (T) with uracil (U), and ends with a termination sequence followed by mRNA modification. The mRNA then exits the nucleus and enters the cytoplasm, where translation occurs on ribosomes; mRNA codons are read by tRNA anticodons to assemble amino acids into a polypeptide chain. This sequence continues until a stop codon is reached, resulting in a new protein.