Translocation of California Red-legged
Frog (Rana draytonii) to Restored Critical
Habitat, Solano County.




Rob Schell, WRA, Inc.
Schell@wra-ca.com, (415) 454-8868
California Red-legged Frog (CRLF)

                 Federally listed as
                  Threatened in 1996
                 CDFW Species of Special
                  Concern (SSC)
                 Final Critical Habitat
                  designated in 2010
                 Recovery Plan Published
                  in 2002
                 Consistent with Recovery
                  Plan actions and tasks
Conservation Banking


The goal of conservation banking is to protect, manage and
enhance/create habitat for ESA listed species. The program
Incentivizes conservation and management of large tracts
private lands to benefit listed species. Once a bank is
developed, the bank owner can sell endangered species
habitat “credits” to mitigate for other project impacts. To
ensure bank success, the owner must establish a non-
wasting endowment for perpetual management of the
preserve which funds scientific based monitoring.
Ridge Top Ranch
Conservation Bank

• 745 acres located in
  southwestern Solano
  County

• 12 existing stock ponds

• Surrounded by privately
  owned Rangeland or
  preserved lands

• Within Designated Critical
  Habitat for CRLF
Historic Anuran Translocations
• California Red-legged Frog
  • GGNRA/USGS
  • Pinnacles National Monument

• Oregon Spotted Frog
     (Rana pretiosa)

• Relict Leopard Frog (Rana onca)

• Northern Leopard Frog
     (Rana pipiens)
Pre-translocation Due Diligence
      at the Conservation Bank Site

                Visual surveys for CRLF in Q1-2 2010

                Surveys were negative

               Follow-up seining for CRLF in Q2 2010

                Surveys were negative

            Toxicology sampling and analysis in Q3 2010

 Analytics were negative for contaminants

Analysis of chytrid fungus at donor location and at Ridge Top Ranch
         Chytrid is present at both sites
Pond D       Ridge Top Ranch
                      Conservation Bank

                           Pond E


Pond B

                            • Ponds N and D were fenced
                              and planted in Q4 2010
                               • Bullrush
                               • Spikerush
                  Pond N       • Arroyo willow

                            • Ponds B and E were fenced
                              and seeded in Q4 2012
                               • Bullrush
                               • Spikerush
Pilot Pond Restoration: Before and After
                      Pond N           Pond D
         Oct. 2010
Before
         March 2012
After
2012 Donor Site Surveys




   Donor larvae
2012: Year 1 Translocation



                                Transported eggs in temperature-
                                controlled coolers




Collected eggmass along with
attachment material.

Placed in large plastic bags
similar to taking home a fish   Eggs placed in hatch-out cages to
from a pet store.               “head-start” larvae and protect
                                against invertebrate predators.
CRLF Embryotic/Larval Development




                                22 days old: Larvae began to hatch


                                41 days old




9 days old: Confirmed embryo                  ~2CM TL
    viability and development
CRLF Larval/Juvenile Development - Continued
                                         108 days old
•   First metamorph
    observed at 138
    days old
                              ~11cm TL
•   Population
    monitoring began                     237 days old:
    Q3 2012 – will                          ~5cm SUL
    continue until 2016


•   All individuals
    captured over
    40mm SUL, were
    PIT tagged to track
    dispersal,
    colonization, and
    demographics
2012 Preliminary Results
                             Number of Metamorph
                           California Red-legged Frogs
   YOY Visually Detected              104

   YOY PIT Tagged                      76

   Males Tagged                        30

   Females Tagged                      16

   Unknown Sex Tagged                  30
2012 YOY R. draytonii Preliminary Demographic
           80.0
                                         Data
           75.0

           70.0

           65.0
                                                                          Male n=30
SUL (mm)




           60.0

           55.0                                                           Female
                                                                          n=16
           50.0
                                                                          Unknown
           45.0                           55 mm is the threshold
                                                                          sex n=30
                                          size at which CRLF can
           40.0                           be reliably sexed.
           35.0

           30.0
                  0.0     10.0     20.0        30.0      40.0      50.0
                                    Weight (grams)
Thank You!
•   Land Bank
•   Dave Kelly (USFWS)
•   Dr. Brad Shaffer (UCLA)
•   WRA Staff
     • Geoff Smick
     • Tricia Valcarcel
     • Dan Chase




                              Rob Schell, WRA, Inc.
                               Schell@wra-ca.com
                              (415) 454-8868 x 149

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Translocation power point for wra website

  • 1. Translocation of California Red-legged Frog (Rana draytonii) to Restored Critical Habitat, Solano County. Rob Schell, WRA, Inc. Schell@wra-ca.com, (415) 454-8868
  • 2. California Red-legged Frog (CRLF) Federally listed as Threatened in 1996 CDFW Species of Special Concern (SSC) Final Critical Habitat designated in 2010 Recovery Plan Published in 2002 Consistent with Recovery Plan actions and tasks
  • 3. Conservation Banking The goal of conservation banking is to protect, manage and enhance/create habitat for ESA listed species. The program Incentivizes conservation and management of large tracts private lands to benefit listed species. Once a bank is developed, the bank owner can sell endangered species habitat “credits” to mitigate for other project impacts. To ensure bank success, the owner must establish a non- wasting endowment for perpetual management of the preserve which funds scientific based monitoring.
  • 4. Ridge Top Ranch Conservation Bank • 745 acres located in southwestern Solano County • 12 existing stock ponds • Surrounded by privately owned Rangeland or preserved lands • Within Designated Critical Habitat for CRLF
  • 5. Historic Anuran Translocations • California Red-legged Frog • GGNRA/USGS • Pinnacles National Monument • Oregon Spotted Frog (Rana pretiosa) • Relict Leopard Frog (Rana onca) • Northern Leopard Frog (Rana pipiens)
  • 6. Pre-translocation Due Diligence at the Conservation Bank Site Visual surveys for CRLF in Q1-2 2010 Surveys were negative Follow-up seining for CRLF in Q2 2010 Surveys were negative Toxicology sampling and analysis in Q3 2010 Analytics were negative for contaminants Analysis of chytrid fungus at donor location and at Ridge Top Ranch Chytrid is present at both sites
  • 7. Pond D Ridge Top Ranch Conservation Bank Pond E Pond B • Ponds N and D were fenced and planted in Q4 2010 • Bullrush • Spikerush Pond N • Arroyo willow • Ponds B and E were fenced and seeded in Q4 2012 • Bullrush • Spikerush
  • 8. Pilot Pond Restoration: Before and After Pond N Pond D Oct. 2010 Before March 2012 After
  • 9. 2012 Donor Site Surveys Donor larvae
  • 10. 2012: Year 1 Translocation Transported eggs in temperature- controlled coolers Collected eggmass along with attachment material. Placed in large plastic bags similar to taking home a fish Eggs placed in hatch-out cages to from a pet store. “head-start” larvae and protect against invertebrate predators.
  • 11. CRLF Embryotic/Larval Development 22 days old: Larvae began to hatch 41 days old 9 days old: Confirmed embryo ~2CM TL viability and development
  • 12. CRLF Larval/Juvenile Development - Continued 108 days old • First metamorph observed at 138 days old ~11cm TL • Population monitoring began 237 days old: Q3 2012 – will ~5cm SUL continue until 2016 • All individuals captured over 40mm SUL, were PIT tagged to track dispersal, colonization, and demographics
  • 13. 2012 Preliminary Results Number of Metamorph California Red-legged Frogs YOY Visually Detected 104 YOY PIT Tagged 76 Males Tagged 30 Females Tagged 16 Unknown Sex Tagged 30
  • 14. 2012 YOY R. draytonii Preliminary Demographic 80.0 Data 75.0 70.0 65.0 Male n=30 SUL (mm) 60.0 55.0 Female n=16 50.0 Unknown 45.0 55 mm is the threshold sex n=30 size at which CRLF can 40.0 be reliably sexed. 35.0 30.0 0.0 10.0 20.0 30.0 40.0 50.0 Weight (grams)
  • 15. Thank You! • Land Bank • Dave Kelly (USFWS) • Dr. Brad Shaffer (UCLA) • WRA Staff • Geoff Smick • Tricia Valcarcel • Dan Chase Rob Schell, WRA, Inc. Schell@wra-ca.com (415) 454-8868 x 149