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Part III:
Biotechnology
“What is genetic engineering?
⪢ A process used by
scientists to
enhance/modify the
characteristics of the
original organism
⪢ Alters the DNA in
organism
Go the classwork section
and complete the Actively
assignment: Can DNA
Editing Save Endangered
Species?
“What are GMO’s?
⪢ Genetically Modified
Organisms
⪢ A plant, animal,
microorganism or other
organism whose genetic
makeup has been modified in
a laboratory using genetic
engineering or transgenic
technology
How do you create recombinant GMO’s?
⪢ Using viruses or bacteria to “infect” animal or plant cells with the
new DNA.
⪢ Coating DNA onto tiny metal pellets, and firing with a special gun
into the cells.
⪢ Injecting the new DNA into fertilized eggs with a very fine needle.
⪢ Using electric shocks to create holes in the membrane covering
sperm and then forcing the new DNA into sperm through these
holes.
“Which type of molecule is
absolutely necessary to
make GMO’s?
⪢ ENZYMES!!! (Proteins)
⪢ Restriction enzymes - cut
DNA at a specific base
sequence
“Do GMO’s occur in nature?
⪢ NO!
⪢ GMO’s are completely
man-made
“What is recombinant DNA?
⪢ DNA that has been taken
from two (or more)
different organisms and
recombined to make a
new DNA sequence that
would not otherwise be
found in the organism
⪢ Created by HUMANS -
NOT NATURE!
How do you create recombinant DNA?
⪢ Remove bacterial DNA (plasmid).
⪢ Cut the Bacterial DNA with “restriction enzymes”.
⪢ Cut the DNA from another organism with “restriction
enzymes”.
⪢ Combine the cut pieces of DNA together with another
enzyme and insert them into bacteria.
⪢ Reproduce the recombinant bacteria.
⪢ The foreign genes will be expressed in the bacteria.
Recombinant
DNA
T
r
a
n
s
f
o
r
m
a
ti
o
n
“What is transformation?
⪢ Inserting a plasmid into a
host cell in order to make
the host cell now express
the gene/DNA in the
plasmid.
“
What are examples of genetic engineering?
⪢ Medical
○ Production of insulin, HGH, etc.
⪢ Environmental
○ Bacteria to clean up oil spills
⪢ Industrial/Manufacturing
⪢ Food/Agriculture
○ BT corn, arctic apples, golden rice
Are GMO’s safe?
According to the Center for Food Safety:
“There is no consensus in the science. A comprehensive review of
peer-reviewed animal feeding studies of GMOs found roughly an equal
number of research groups raising concerns about genetically
engineered foods and those suggesting GMOs were as safe and
nutritious as conventional foods. The review also found that most
studies finding GMOs foods the same as conventional foods were
performed by biotechnology companies or their associates...”
Go the classwork section
and complete the Actively
assignment: Are GMO’s
Bad? Science Says They’re
Safe
Click on the link when you’re ready to take the
quiz: Unit 4 - Week 6 Checkpoint Quiz
It’s QUIZ time!
You smarty pants will
have no trouble...Good
luck!
point Quiz

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Unit 4 week 6 slides

  • 2. “What is genetic engineering? ⪢ A process used by scientists to enhance/modify the characteristics of the original organism ⪢ Alters the DNA in organism
  • 3. Go the classwork section and complete the Actively assignment: Can DNA Editing Save Endangered Species?
  • 4. “What are GMO’s? ⪢ Genetically Modified Organisms ⪢ A plant, animal, microorganism or other organism whose genetic makeup has been modified in a laboratory using genetic engineering or transgenic technology
  • 5. How do you create recombinant GMO’s? ⪢ Using viruses or bacteria to “infect” animal or plant cells with the new DNA. ⪢ Coating DNA onto tiny metal pellets, and firing with a special gun into the cells. ⪢ Injecting the new DNA into fertilized eggs with a very fine needle. ⪢ Using electric shocks to create holes in the membrane covering sperm and then forcing the new DNA into sperm through these holes.
  • 6. “Which type of molecule is absolutely necessary to make GMO’s? ⪢ ENZYMES!!! (Proteins) ⪢ Restriction enzymes - cut DNA at a specific base sequence
  • 7. “Do GMO’s occur in nature? ⪢ NO! ⪢ GMO’s are completely man-made
  • 8. “What is recombinant DNA? ⪢ DNA that has been taken from two (or more) different organisms and recombined to make a new DNA sequence that would not otherwise be found in the organism ⪢ Created by HUMANS - NOT NATURE!
  • 9. How do you create recombinant DNA? ⪢ Remove bacterial DNA (plasmid). ⪢ Cut the Bacterial DNA with “restriction enzymes”. ⪢ Cut the DNA from another organism with “restriction enzymes”. ⪢ Combine the cut pieces of DNA together with another enzyme and insert them into bacteria. ⪢ Reproduce the recombinant bacteria. ⪢ The foreign genes will be expressed in the bacteria.
  • 11. “What is transformation? ⪢ Inserting a plasmid into a host cell in order to make the host cell now express the gene/DNA in the plasmid.
  • 12.
  • 13. What are examples of genetic engineering? ⪢ Medical ○ Production of insulin, HGH, etc. ⪢ Environmental ○ Bacteria to clean up oil spills ⪢ Industrial/Manufacturing ⪢ Food/Agriculture ○ BT corn, arctic apples, golden rice
  • 14. Are GMO’s safe? According to the Center for Food Safety: “There is no consensus in the science. A comprehensive review of peer-reviewed animal feeding studies of GMOs found roughly an equal number of research groups raising concerns about genetically engineered foods and those suggesting GMOs were as safe and nutritious as conventional foods. The review also found that most studies finding GMOs foods the same as conventional foods were performed by biotechnology companies or their associates...”
  • 15. Go the classwork section and complete the Actively assignment: Are GMO’s Bad? Science Says They’re Safe
  • 16. Click on the link when you’re ready to take the quiz: Unit 4 - Week 6 Checkpoint Quiz It’s QUIZ time! You smarty pants will have no trouble...Good luck! point Quiz