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Validated Method List

KCAS Bioanalytical & Biomarker Services , profile picture
KCAS Bioanalytical & Biomarker Services

The document lists new and under development analytical methods for various analytes in different biological matrices like plasma, serum, urine, etc. It provides the analyte, matrix, lower limit of quantification, unit of measurement, and analytical platform (LC-MS/MS, ELISA, HPLC, etc.) for over 100 methods. The methods cover therapeutic drug monitoring, toxicology testing, biomarker analysis, and more. New methods are fully validated, while those under development are still in the validation process.

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Validated Method List New Methods & Methods Under Development KCAS Bioanalytical Services | kcasbio.com 1 New Methods Methods Under Development Analyte Matrix LLOQ Unit Platform Amlodipine Human Plasma 0.15 ng/mL LC-MS/MS Guaifenesin Human Plasma 10 ng/mL LC-MS/MS Methylphenidate Human Plasma 0.1 ng/mL LC-MS/MS Terbinafine Human Nail 200 mcg/g LC-MS/MS Zolmitriptan / N-Desmethylzolmitriptan Human Plasma 0.1 / 0.1 ng/mL LC-MS/MS Analyte Matrix LLOQ Unit Platform ACTH Intact 1-39 Human Plasma 7 pg/mL ELISA Amantadine Human Plasma 2 ng/mL LC-MS/MS L-Amphetamine / D-Amphetamine Human Plasma 0.2 / 0.25 ng/mL LC-MS/MS C-Reactive Protein Human Plasma 62.5 ng/mL ELISA Inflammatory Cytokine Panel: 4 to 9-plex Human Plasma Inquire ELISA Interferon b1 Human Plasma 2 pg/mL ELISA Interferon, gamma- Human Plasma 15 pg/mL ELISA Interleukin-6 (IL-6) Human Plasma & Serum 1.56 pg/mL ELISA PTH 1-34 Human Plasma 10 pg/mL ELISA PTH 1-84 Intact Human Plasma 10 pg/mL ELISA Testosterone Rat & Mini Pig Plasma 0.3 ng/mL LC-MS
Validated Method List 2 Analyte Matrix LLOQ Unit Platform Acebutolol / Diacetolol Human Plasma 5 ng/mL HPLC/UV Acetaminophen (APAP) Human Plasma 0.1 mcg/mL LC-MS/MS Acetaminophen / Tramadol Human Plasma 100 / 5 ng/mL LC-MS/MS Acetylsalicylic Acid / Salicylic Acid Human Plasma 0.25/ 0.75 mcg/mL HPLC/UV Acid Glycoprotein, α1 Human Plasma 3.5 ng/mL ELISA Acyclovir Human Plasma 20 ng/mL HPLC/FL Acyclovir Human Urine 1 mcg/mL HPLC/FL Adipenectin Human Serum 0.8 ng/mL ELISA Alkaline Hematin (see Menstrual Blood) Menstrual Products Allopurinol / Oxypurinol Human Plasma 50 / 100 ng/mL LC-MS/MS Alprazolam / α-Hydroxyalprazolam Dog Plasma 0.5 / 0.5 ng/mL LC-MS/MS Alprazolam / α-Hydroxyalprazolam Human Plasma 0.5 / 0.5 ng/mL LC-MS/MS Aluminum Human Plasma 5 / 2 ng/mL HPLC Aluminum Human Urine 5 / 2 ng/mL HPLC Amikacin Human Serum 0.15 mcg/mL LC-MS/MS Amikacin Human Urine 0.5 mcg/mL LC-MS/MS Aminosalicylic Acid, 5- Human Feces 1 ng on col HPLC/FL Aminosalicylic Acid, 5- Human Urine 2 mcg/mL HPLC/FL Aminosalicylic Acid, 5- Rat Plasma 10 ng/mL LC-MS/MS Aminosalicylic Acid, 5- / N-Acetyl-5-Aminosalicylic Acid Human Plasma 10 / 10 ng/mL HPLC/FL Amiodarone / Desethylamiodarone Human Plasma 10 ng/mL LC-MS/MS Amitriptyline Human Plasma 200 pg/mL LC-MS/MS Amlodipine Human Plasma 50 pg/mL LC-MS/MS Amlodipine Human Plasma 0.15 ng/mL LC-MS/MS Anipamil Human Plasma 5 ng/mL HPLC/FL Aprepitant Human Plasma 10 ng/mL LC-MS/MS Aspirin (See Acetylsalicylic Acid) Human Plasma 0.25 ng/mL HPLC/UV Atenolol Human Plasma 10 ng/mL LC-MS/MS Atenolol Human Urine 250 ng/mL LC-MS/MS Atenolol, (R)- and (S)- Human Plasma 20 / 20 ng/mL HPLC/FL Atenolol, (R)- and (S)- Rat Plasma 20 / 20 ng/mL HPLC/FL Atomoxetine Human Plasma 10 ng/mL LC-MS/MS Atorvastatin / p- and o-Hydroxyatorvastatin Human Plasma 0.2 / 0.2 / 0.2 ng/mL LC-MS/MS Azacytidine, 5- Human Plasma 10 ng/mL LC-MS/MS Azithromycin Human Plasma 2 ng/mL LC-MS/MS Benazepril/ Benazeprilat Dog Plasma 0.1 / 0.5 ng/mL LC-MS/MS Benazepril / Benazeprilat Human Plasma 2 / 2 ng/mL LC-MS/MS Bicalutamide Human Plasma 2.5 ng/mL LC-MS/MS Bucladesine Human Plasma 1 ng/mL LC-MS/MS Brompheniramine / Chlorpheniramine / Pseudeophedrine Human Plasma 0.5 / 0.5 / 5 ng/mL LC-MS/MS Buprenorphine / Norbuprenorphine Dog Plasma 100 pg/mL LC-MS/MS
Validated Method List 3 Analyte Matrix LLOQ Unit Platform Buprenorphine / Norbuprenorphine Human Plasma 50 pg/mL LC-MS/MS Buprenorphine / Norbuprenorphine Rabbit Plasma 50 pg/mL LC-MS/MS Buspirone / 1-PP Dog Plasma 100 pg/mL LC-MS/MS Buspirone / 1-PP Human Plasma 100 pg/mL LC-MS/MS Butalbital Human Plasma 0.1 mcg/mL HPLC/UV Butorphanol Human Plasma 20 pg/mL LC-MS/MS Caffeine Human Plasma 0.05 mcg/mL HPLC/UV Canrenone Human Plasma 2 ng/mL LC-MS/MS Canrenone Human Urine 20 ng/mL LC-MS/MS Captisol Human Plasma 5 mcg/mL LC-MS/MS Carbamazepine Human Plasma 0.05 mcg/mL HPLC/UV Carisbamate Human Plasma 5 ng/mL LC-MS/MS Cetirizine Human Plasma 2 ng/mL LC-MS/MS Chlorpheniramine Human Plasma 0.5 ng/mL LC-MS/MS Cimetidine Dog Plasma 50 ng/mL HPLC/UV Cimetidine Human Plasma 50 ng/mL HPLC/UV Ciprofloxacin Cat Plasma 25 ng/mL HPLC/FL Ciprofloxacin Human Serum 10 ng/mL LC-MS/MS Ciprofloxacin Human Plasma 10 ng/mL LC-MS/MS Cisapride Human Plasma 5 ng/mL HPLC/FL Citric Acid Human Urine 50 mcg/mL LC-MS/MS Clarithromycin Human Plasma 10 ng/mL LC-MS/MS Clonazepam / 7-Hydroxyclonazepam Human Plasma 0.1 / 0.2 pg/mL LC-MS/MS Clonidine Human Plasma 10 pg/mL LC-MS/MS Clotrimazole / Ketoconazole Human Plasma 5 / 50 ng/mL LC-MS/MS Cluster of Differentiation 14 (CD-14) Human Plasma 100 pg/mL ELISA Codeine Human Plasma 1 ng/mL LC-MS/MS Cordycepin Human Plasma 1 ng/mL LC-MS/MS Cortisol Human Plasma 40.4 ng/mL LC-MS/MS Cortisone Human Plasma 5 ng/mL HPLC/UV Cyclobenzaprine Dog Plasma 0.1 ng/mL LC-MS/MS Cyclobenzaprine Human Plasma 0.2 ng/mL LC-MS/MS Cytarabine (Ara-C) Human Plasma 20 ng/mL LC-MS/MS Deoxycholic Acid Human Plasma 10 ng/mL LC-MS/MS Dexamethasone Human Plasma 0.25 ng/mL LC-MS/MS Dextromethorphan Human Plasma 50 pg/mL LC-MS/MS Dextromethorphan / 3-Hydroxymorphinan / 4-Methoxymorphinan Human Plasma 10 ng/mL HPLC/FL Dextromethorphan / 3-Hydroxymorphinan / 4-Methoxymorphinan Human Urine 0.25 mcg/mL HPLC/FL Dextrorphan Human Plasma 10 ng/mL HPLC/FL Dextrorphan Human Urine 250 ng/mL HPLC/FL Diclofenac Human Plasma 5 ng/mL LC-MS/MS Diclofenac Rat Plasma 10 ng/mL HPLC/UV
Validated Method List 4 Analyte Matrix LLOQ Unit Platform Digoxin Human Plasma 0.1 ng/mL LC-MS/MS Diltiazem / N-Desmethyldiltiazem / Desacetyldiltiazem Human Plasma 4 / 2 / 1 ng/mL LC-MS/MS Diphenydramine Human Plasma 1 ng/mL LC-MS/MS Dipyridamole Human Plasma 50 ng/mL LC-MS/MS DNA Quantitation Human Tissue 50 ng/mL Fluorometric Doxepin / Nordoxepin Human Urine 0.25 / 0.20 ng/mL LC-MS/MS Doxorubicin Human Urine 1.5 ng/mL LC-MS/MS Doxorubicin/Doxorubicinol Human Plasma 0.5 / 0.5 ng/mL LC-MS/MS Doxylamine Human Plasma 5 / 100 ng/mL HPLC/UV Doxylamine Human Urine 5 / 100 ng/mL HPLC/UV Dyclonine Dog Serum 1 ng/mL LC-MS/MS Ebastine Human Plasma 10 ng/mL HPLC/UV Efavirenz Human Plasma 50 ng/mL LC-MS/MS Enalapril / Enalaprilat Human Plasma 0.25 ng/mL LC-MS/MS Esomeprazole Human Plasma 0.25 ng/mL LC-MS/MS Ethambutol Human DBS 100 ng/mL LC-MS/MS Famotidine Human Plasma 2 ng/mL LC-MS/MS Famotidine Human Urine 2 ng/mL LC-MS/MS Felodipine Human Plasma 25 pg/mL LC-MS/MS Femoxitine / N-Desmethylfemoxitine Human Plasma 5 ng/mL HPLC/FL Femoxitine / N-Desmethylfemoxitine Monkey Plasma 5 ng/mL HPLC/FL Fenofibric Acid Human Plasma 20 ng/mL LC-MS/MS Fexofenadine Human Plasma 1 ng/mL LC-MS/MS Fexofenadine Mouse Plasma 25 ng/mL LC-MS/MS Fexofenadine Rabbit Plasma 25 ng/mL LC-MS/MS Fexofenadine Rat Plasma 25 ng/mL LC-MS/MS Flavoxate / MFCA metabolite Human Plasma 0.1 ng/mL LC-MS/MS Flurbiprofen Human Plasma 5 ng/mL LC-MS/MS Flurbiprofen, (R)- and (S)- Human Plasma 0.25 mcg/mL HPLC/FL Flurbiprofen, (R)- and (S)- Human Urine 0.5 mcg/mL HPLC/FL Furosemide Human Plasma 50 ng/mL LC-MS/MS Furosemide Human Urine 50 ng/mL LC-MS/MS Gemcitabine Human Plasma 40 ng/mL HPLC/UV Gemcitabine Human Urine 40 ng/mL HPLC/UV Gemfibrozil Human Plasma 0.5 mcg/mL HPLC/UV Ghrelin Active Human Plasma 100 pg/mL ELISA Ghrelin Total Human Plasma 200 pg/mL ELISA Gliclazide Human Plasma 100 ng/mL HPLC/UV Granisetron Dog Plasma 0.1 ng/mL LC-MS/MS Guaifenesin / Naproxen Human Plasma 10 / 250 ng/mL LC-MS/MS Haloperidol Human Plasma 25 pg/mL LC-MS/MS Hepatocyte Growth Factor (HGF) Human Plasma 400 pg/mL ELISA Heptazyme Antibodies Monkey Serum 1:25,600 titer ELISA
Validated Method List 5 Analyte Matrix LLOQ Unit Platform Homocysteine Dog Plasma 5 mcg/mL LC-MS/MS Homocysteine Rat Plasma 5 mcg/mL LC-MS/MS Hydrochlorothiazide (HCTZ) Human Plasma 1 ng/mL HPLC/UV Hydrochlorothiazide (HCTZ) Human Urine 0.05 mcg/mL HPLC/UV Hydrocodone Human Plasma 0.1 ng/mL LC-MS/MS Hydromorphone Dog Plasma 20 pg/mL LC-MS/MS Hydromorphone Human Plasma 40 pg/mL LC-MS/MS Hydroxymephenytoin, (R)-4- / Hydroxymephenytoin, (S)-4- Human Plasma 10 ng/mL LC-MS/MS Hydroxymephenytoin, (R)-4- / Hydroxymephenytoin, (S)-4- Human Saliva 10 ng/mL LC-MS/MS Hydroxymephenytoin, (R)-4- / Hydroxymephenytoin, (S)-4- Human Urine 0.1 mcg/mL LC-MS/MS Hydroxymidazolam, α- / Hydroxymidazolam, 4- Dog Plasma 2 / 2 ng/mL LC-MS/MS Hydroxymidazolam, α- / Hydroxymidazolam, 4- Rat Plasma 2 / 2 ng/mL LC-MS/MS Ibuprofen Human Plasma 0.25 mcg/mL LC-MS/MS Ibuprofen, (R)- and (S)- Human Plasma 0.25 mcg/mL HPLC/UV Ibuprofen, (R)- and (S)- Human Urine 2.5 mcg/mL HPLC/FL Imipramine / Desipramine Human Plasma 0.1 ng/mL LC-MS/MS Indocyanine Green (ICG) Human Plasma 0.3 mcg/mL HPLC/UV Indomethacin Human Plasma 10 ng/mL LC-MS/MS Indomethacin Plasma/Serum 20 ng/mL HPLC/FL Interferon alpha Human Serum 10 pg/mL ELISA Isotretinoin / 4-Oxo-Isotretinoin Human Plasma 10 ng/mL LC-MS/MS Isradapine Human Plasma 0.1 ng/mL LC-MS/MS Ketoconazole / Clotrimazole Human Plasma 50 / 5 ng/mL LC-MS/MS Ketoprofen Human Plasma 0.25 ng/mL LC-MS/MS Lansoprazole Human Plasma 0.25 ng/mL LC-MS/MS Levofloxacin Rabbit Plasma 10 ng/mL LC-MS/MS Lorazepam Human Plasma 0.2 ng/mL LC-MS/MS Losartan / Carboxylosartan Human Plasma 2 / 2 ng/mL LC-MS/MS Meloxicam Human Plasma 10 ng/mL LC-MS/MS Melphalan Human Plasma 25 ng/mL LC-MS/MS Memantine Human Plasma 0.5 ng/mL LC-MS/MS Memantine N-Glucuronide Human Plasma 0.2 ng/mL LC-MS/MS Menstrual Blood (as Alkaline Hematin) Menstrual Products 1 mL Colorimetric Mephenytoin, (R)- / Mephenytoin, (S)- Human Plasma 10 ng/mL LC-MS/MS Mephenytoin, (R)- / Mephenytoin, (S)- Human Saliva 10 ng/mL LC-MS/MS Mephenytoin, (R)- / Mephenytoin, (S)- Human Urine 0.1 mcg/mL LC-MS/MS Mercaptopurine, 6- / 6-Thiouric Acid Human Plasma 2 / 20 ng/mL LC-MS/MS Mercaptopurine, 6- / 6-Thiouric Acid Human Urine 6 / 60 ng/mL LC-MS/MS Metformin Human Plasma 20 ng/mL LC-MS/MS Methocarbamol Human Plasma 0.1 mcg/mL HPLC/FL Methylnicotinamide Human Plasma 10 ng/mL LC-MS/MS
Validated Method List 6 Analyte Matrix LLOQ Unit Platform Methylphenidate Human Plasma 0.1 ng/mL LC-MS/MS Methylprednisolone Human Plasma 2.5 ng/mL LC-MS/MS Metoclopramide Human Plasma 1 ng/mL HPLC/UV Metoclopramide Human Urine 60 ng/mL HPLC/FL Metoprolol Human Plasma 1 ng/mL LC-MS/MS Metoprolol / Hydroxymetoprolol Human Plasma 2.5 ng/mL HPLC/FL Metoprolol / Hydroxymetoprolol Human Urine 1000 ng/mL HPLC/FL Midazolam / α-Hydroxymidazolam Human Plasma 0.5 / 0.5 ng/mL LC-MS/MS Midazolam / 1-Hydroxymidazolam / 4-Hydroxymidazolam Human Urine 10 / 2 / 2 ng/mL LC-MS/MS Midazolam / α-Hydroxymidazolam Human Urine 20 / 0.5 ng/mL LC-MS/MS Molindone Human Plasma 0.25 ng/mL LC-MS/MS Monocyte Chemotactic Protein 1 (MCP-1) Human Plasma 30 pg/mL ELISA Monocyte Chemotactic Protein 3 (MCP-3) Human Plasma 15 pg/mL ELISA Morphine / Morphine-3-Glucuronide / Morphine-6-Glucuronide Dog Plasma 0.5 / 5 / 2 ng/mL LC-MS/MS Morphine / Morphine-3-Glucuronide / Morphine-6-Glucuronide Human Plasma 0.5 / 5 / 2 ng/mL LC-MS/MS Morphine / Morphine-3-Glucuronide / Morphine-6-Glucuronide Ovine Plasma 0.5 / 5 / 2 ng/mL LC-MS/MS Moxifloxacin Human DBS 100 ng/mL LC-MS/MS Moxifloxacin Human Plasma 20 ng/mL LC-MS/MS Naproxen Human Plasma 0.25 mcg/mL LC-MS/MS Niacin (Nicotinic Acid) / Nicotinuric Acid Human Urine 25 ng/mL LC-MS/MS Nifedipine / NPO Human Plasma 1 ng/mL LC-MS/MS Nitrofurantoin Human Plasma 5 ng/mL HPLC/UV Nitrofurantoin Human Urine 2 mcg/mL HPLC/UV Noroxycodone Human Plasma 0.25 ng/mL LC-MS/MS Olanzapine Human Plasma 0.1 ng/mL LC-MS/MS Omeprazole Human Plasma 5 ng/mL LC-MS/MS Ondansetron Human Plasma 0.5 ng/mL LC-MS/MS Oxandrolone Human Plasma 0.25 ng/mL LC-MS/MS Oxaprozin Human Plasma 2 mcg/mL HPLC/UV Oxybutynin / N-Desethyloxybutynin Human Plasma 50 / 250 pg/mL LC-MS/MS Oxycodone / Noroxycodcone / Oxymorphone Human Plasma 0.25 ng/mL LC-MS/MS Oxymorphone Human Plasma 0.25 ng/mL LC-MS/MS Paclitaxel Human Plasma 100 pg/mL LC-MS/MS Paclitaxel Human Urine 5 ng/mL LC-MS/MS Pantoprazole Human Plasma 10 ng/mL LC-MS/MS Paraxanthine Human Plasma 25 ng/mL HPLC/UV Paroxetine Human Plasma 0.2 ng/mL LC-MS/MS Pentazocine Human Plasma 1 ng/mL LC-MS/MS Phenylephrine Human Plasma 0.5 ng/mL LC-MS/MS Phenylephrine Human Urine 5 ng/mL LC-MS/MS
Validated Method List 7 Analyte Matrix LLOQ Unit Platform Phenytoin Human Plasma 0.25 mcg/mL HPLC/UV Phenytoin Human Urine 1 mcg/mL HPLC/UV Phenytoin / Phenylhydantoin / Phenobarbitol (Free Assay) Human Urine 0.1 mcg/mL HPLC/UV Pindolol Human Plasma 1 ng/mL HPLC/UV Piroxicam Human Plasma 50 ng/mL HPLC/UV Potassium Human Urine 8 mM ICP-MS Prednisolone Human Plasma 1 ng/mL HPLC/UV Prednisone Human Plasma 1 ng/mL HPLC/UV Primidone Human Plasma 25 ng/mL LC-MS/MS Propafenone / N-Despropylpropafenone- / 5-Hydroxypropafenone Human Plasma 250 pg/mL LC-MS/MS Propofol Human Plasma 2.5 ng/mL HPLC/FL Propoxyphene/Norpropoxyphene Human Plasma 1 ng/mL LC-MS/MS Propranolol / 4-Hydroxypropranolol Human Plasma 2.5 ng/mL HPLC/FL Propranolol Glucuronide Human Urine 1 mcg/mL HPLC/FL Pseudoephedrine Human Plasma 5 ng/mL LC-MS/MS Pyrazinamide Human DBS 10 mcg/mL LC-MS/MS Quinidine Human Plasma 10 ng/mL HPLC/UV Ranitidine Human Plasma 5 ng/mL HPLC/UV Reserpine Human Plasma 25 pg/mL HPLC/FL Resistin Human Plasma 160 ng/mL ELISA Ribavirin Human Plasma 5 ng/mL LC-MS/MS Rifampin Human DBS 200 ng/mL LC-MS/MS Risperidone Human Plasma 0.1 ng/mL LC-MS/MS Ropinirole Human Plasma 20 pg/mL LC-MS/MS Ropinirole Mini-Pig Plasma 20 pg/mL LC-MS/MS Rosuvastatin Human Plasma 0.2 ng/mL LC-MS/MS Salicylic Acid Plasma 0.7 mcg/mL HPLC/FL Salicylic Acid Urine 9.2 mcg/mL HPLC/FL Salicyluric Acid Human Plasma 25 ng/mL HPLC/UV Salicyluric Acid Human Urine 2.5 mcg/mL HPLC/UV Simvastatin / Simvastatin Hydroxy Acid Human Plasma 50 / 50 pg/mL LC-MS/MS Spironolactone Human Plasma 0.5 ng/mL LC-MS/MS Sumatriptan Human Plasma 0.5 ng/mL LC-MS/MS Tacrolimus Rat DBS 5 ng/mL LC-MS/MS Tacrolimus Dog Whole Blood 0.1 ng/mL LC-MS/MS Tacrolimus Human Whole Blood 0.5 ng/mL LC-MS/MS Temazepam Human Plasma 20 ng/mL LC-MS/MS Tenofovir Human Plasma 10 ng/mL LC-MS/MS Tenoxicam Human Plasma 20 ng/mL HPLC/UV
Validated Method List 8 Analyte Matrix LLOQ Unit Platform Terbinafine Human Nail 200 mcg/g LC-MS/MS Terbinafine / N-Desmethylterbinafine Human Plasma 40 pg/mL LC-MS/MS Testosterone (total) Human Serum 5 ng/dL RIA Theophylline Human Plasma 50 ng/mL HPLC/UV Theophylline Human Plasma 0.2 mcg/mL HPLC/UV Theophylline Human Serum 0.2 mcg/mL HPLC/UV Tizanidine Human Plasma 100 pg/mL LC-MS/MS Tolbutamide / Carboxytolbutamide / Hydroxytolbutamide Human Plasma 0.25 / 0.5 / 0.5 mcg/mL HPLC/UV Tolbutamide / Carboxytolbutamide / Hydroxytolbutamide Human Urine 0.25 / 0.5 / 0.5 mcg/mL HPLC/UV Tramadol Dog Plasma 2 ng/mL LC-MS/MS Tramadol / Acetaminophen Human Plasma 2.5 / 100 ng/mL LC-MS/MS Tretinoin / Isotretinoin Human Plasma 10 / 10 ng/mL LC-MS/MS Triamcinolone Human Plasma 20 pg/mL LC-MS/MS Triamterene Human Plasma 2 ng/mL LC-MS/MS Tyramine Human Plasma 1 ng/mL LC-MS/MS Valproic Acid Human Plasma 0.5 mcg/mL LC-MS/MS Venlafaxine / O-Desmethylvenlafaxine Human Plasma 2.5 ng/mL LC-MS/MS Vitamin B6 Human Plasma 50 ng/mL LC-MS/MS Vitamin E Human Plasma 0.5 mcg/mL HPLC/UV Warfarin, (R)- and (S)- Human Plasma 50 ng/mL HPLC/FL Xanthine Human Urine 1 mcg/mL HPLC/UV Xanthine Human Plasma 0.5 mcg/mL HPLC/UV Zolpidem Human Plasma 0.5 ng/mL LC-MS/MS Zolmitriptan / N-Desmethylzolmitriptan Human Plasma 0.1 / 0.1 ng/mL LC-MS/MS Zonisamide Human Serum 50 ng/mL HPLC/UV
Analgesic Methods 9 Analyte Matrix LLOQ Unit Platform Acetaminophen (APAP) Human Plasma 0.1 mcg/mL LC-MS/MS Acetaminophen / Tramadol Human Plasma 100 / 5 ng/mL LC-MS/MS Acetylsalicylic Acid / Salicylic Acid Human Plasma 0.25 / 0.75 mcg/mL HPLC/UV Buprenorphine / Norbuprenorphine Dog Plasma 100 pg/mL LC-MS/MS Buprenorphine / Norbuprenorphine Human Plasma 50 pg/mL LC-MS/MS Buprenorphine / Norbuprenorphine Rabbit Plasma 50 pg/mL LC-MS/MS Butorphanol Human Plasma 20 pg/mL LC-MS/MS Codeine Human Plasma 1 ng/mL LC-MS/MS Diclofenac Human Plasma 5 ng/mL LC-MS/MS Flurbiprofen Human Plasma 5 ng/mL LC-MS/MS Flurbiprofen, (R)- and (S)- Human Plasma 0.25 mcg/mL HPLC/FL Flurbiprofen, (R)- and (S)- Human Urine 0.5 mcg/mL HPLC/FL Hydrocodone Human Plasma 0.1 ng/mL LC-MS/MS Hydromorphone Dog Plasma 20 pg/mL LC-MS/MS Hydromorphone Human Plasma 20 pg/mL LC-MS/MS Ibuprofen Human Plasma 0.25 mcg/mL LC-MS/MS Ibuprofen, (R)- and (S)- Human Plasma 0.25 mcg/mL HPLC/UV Ibuprofen, (R)- and (S)- Human Urine 2.5 mcg/mL HPLC/FL Indomethacin Human Plasma 10 ng/mL LC-MS/MS Ketoprofen Human Urine 1000 ng/mL HPLC Ketoprofen Human Plasma 0.25 ng/mL LC-MS/MS Mefenamic Acid Human Plasma 40 ng/mL LC-MS/MS Meloxicam Human Plasma 10 ng/mL LC-MS/MS Morphine / Morphine-3-Glucuronide / Morphine-6-Glucuronide Dog Plasma 0.5 / 5 / 2 ng/mL LC-MS/MS Morphine / Morphine-3-Glucuronide / Morphine-6-Glucuronide Human Plasma 0.5 / 5 / 2 ng/mL LC-MS/MS Morphine / Morphine-3-Glucuronide / Morphine-6-Glucuronide Ovine Plasma 0.5 / 5 / 2 ng/mL LC-MS/MS Naproxen Human Plasma 0.25 mcg/mL LC-MS/MS Oxaprozin Human Plasma 2 mcg/mL HPLC/UV Noroxycodone Human Plasma 0.25 ng/mL LC-MS/MS Oxycodone / Noroxycodcone / Oxymorphone Human Plasma 0.25 ng/mL LC-MS/MS Oxymorphone Human Plasma 0.25 ng/mL LC-MS/MS Pentazocine Human Plasma 1 ng/mL LC-MS/MS Piroxicam Human Plasma 50 ng/mL HPLC/UV Propoxyphene / Norpropoxyphene Human Plasma 1 ng/mL LC-MS/MS Salicylic Acid Plasma 0.7 mcg/mL HPLC/FL Salicylic Acid Urine 9.2 mcg/mL HPLC/FL Salicyluric Acid Human Plasma 25 ng/mL HPLC/UV Salicyluric Acid Human Urine 2.5 mcg/mL HPLC/UV Tenoxicam Human Plasma 20 ng/mL HPLC/UV Tramadol Dog Plasma 2 ng/mL LC-MS/MS Tramadol / Acetaminophen Human Plasma 2.5 / 100 ng/mL LC-MS/MS
Animal Methods 10 Analyte Matrix LLOQ Unit Platform Alprazolam / α-Hydroxyalprazolam Dog Plasma 0.5 ng/mL LC-MS/MS Aminosalicylic Acid, 5- Rat Plasma 10 ng/mL LC-MS/MS Atenolol, (R)- and (S)- Rat Plasma 20 / 20 ng/mL HPLC/FL Benazepril/ Benazeprilat Dog Plasma 0.1 / 0.5 ng/mL LC-MS/MS Buprenorphine / Norbuprenorphine Dog Plasma 100 pg/mL LC-MS/MS Buprenorphine / Norbuprenorphine Rabbit Plasma 50 pg/mL LC-MS/MS Buspirone / 1-PP Dog Plasma 100 pg/mL LC-MS/MS Cimetidine Dog Plasma 50 ng/mL HPLC/UV Ciprofloxacin Cat Plasma 25 ng/mL HPLC/FL Cyclobenzaprine Dog Plasma 0.1 ng/mL LC-MS/MS Diclofenac Rat Plasma 10 ng/mL HPLC/UV Dyclonine Dog Serum 1 ng/mL LC-MS/MS Femoxitine / N-Desmethylfemoxitine Monkey Plasma 5 ng/mL HPLC/FL Fexofenadine Mouse Plasma 25 ng/mL LC-MS/MS Fexofenadine Rabbit Plasma 25 ng/mL LC-MS/MS Fexofenadine Rat Plasma 25 ng/mL LC-MS/MS Granisetron Dog Plasma 0.1 ng/mL LC-MS/MS Homocysteine Dog Plasma 5 mcg/mL LC-MS/MS Homocysteine Rat Plasma 5 mcg/mL LC-MS/MS Hydromorphone Dog Plasma 20 pg/mL LC-MS/MS Hydromorphone Dog Serum 20 pg/mL LC-MS/MS Hydroxymidazolam, α- / Hydroxymidazolam, 4- Dog Plasma 2 / 2 ng/mL LC-MS/MS Hydroxymidazolam, α- / Hydroxymidazolam, 4- Rat Plasma 2 / 2 ng/mL LC-MS/MS Levofloxacin Rabbit Plasma 10 ng/mL LC-MS/MS Lidocaine Dog Plasma 0.1 ng/mL LC-MS/MS Lidocaine Dog Urine 0.1 ng/mL LC-MS/MS Lidocaine Dog Tissues 10 ng/g LC-MS/MS Lidocaine Rat Plasma 0.5 ng/mL LC-MS/MS Morphine / Morphine-3-Glucuronide / Morphine- 6-Glucuronide Dog Plasma 0.5 / 5 / 2 ng/mL LC-MS/MS Morphine / Morphine-3-Glucuronide / Morphine- 6-Glucuronide Ovine Plasma 0.5 / 5 / 2 ng/mL LC-MS/MS Ropinirole Mini-Pig Plasma 20 pg/mL LC-MS/MS Tacrolimus Dog Whole Blood 0.1 ng/mL LC-MS/MS Tramadol Dog Plasma 2 ng/mL LC-MS/MS
Biomarkers 11 Analyte Matrix LLOQ Unit Platform Acid Glycoprotein, α1 Human Plasma 3.5 ng/mL ELISA Adipenectin Human Serum 0.8 ng/mL ELISA Cluster of Differentiation 14 (CD-14) Human Plasma 100 pg/mL ELISA Cortisol Human Plasma 40.4 ng/mL LC-MS/MS Cortisone Human Plasma 5 ng/mL HPLC/UV Deoxycholic Acid Human Plasma 10 ng/mL LC-MS/MS Ghrelin Human Serum 94 pg/mL ELISA Ghrelin Active Human Plasma 100 pg/mL ELISA Ghrelin Total Human Plasma 200 pg/mL ELISA Hepatocyte Growth Factor (HGF) Human Plasma 400 pg/mL ELISA Homocysteine Dog Plasma 5 mcg/mL LC-MS/MS Homocysteine Rat Plasma 5 mcg/mL LC-MS/MS Monocyte Chemotactic Protein 1 (MCP-1) Human Plasma 30 pg/mL ELISA Monocyte Chemotactic Protein 3 (MCP-3) Human Plasma 15 pg/mL ELISA Methylnicotinamide Human Plasma 10 ng/mL LC-MS/MS Resistin Human Plasma 160 ng/mL ELISA Tyramine Human Plasma 1 ng/mL LC-MS/MS Vitamin B6 Human Plasma 50 ng/mL LC-MS/MS Vitamin E Human Plasma 0.5 mcg/mL HPLC/UV Xanthine Human Plasma 0.5 mcg/mL HPLC/UV Xanthine Human Urine 1 mcg/mL HPLC/UV
Cardiovascular Methods 12 Analyte Matrix LLOQ Unit Platform Acebutolol / Diacetolol Human Plasma 5 ng/mL HPLC/UV Amiodarone / Desethylamiodarone Human Plasma 10 ng/mL LC-MS/MS Amlodipine Human Plasma 50 pg/mL LC-MS/MS Anipamil Human Plasma 5 ng/mL HPLC/FL Atenolol Human Plasma 10 ng/mL LC-MS/MS Atenolol Human Urine 250 ng/mL LC-MS/MS Atenolol, (R)- and (S)- Human Plasma 20 ng/mL HPLC/FL Atenolol, (R)- and (S)- Rat Plasma 20 ng/mL HPLC/FL Benazepril / Benazeprilat Dog Plasma 0.1 / 0.5 ng/mL LC-MS/MS Benazepril / Benazeprilat Human Plasma 2 / 2 ng/mL LC-MS/MS Bucladesine Human Plasma 1 ng/mL LC-MS/MS Digoxin Human Plasma 0.1 ng/mL LC-MS/MS Diltiazem / N-Desmethyldiltiazem / Desacetyldiltiazem Human Plasma 4 / 2 / 1 ng/mL LC-MS/MS Dipyridamole Human Plasma 50 ng/mL LC-MS/MS Enalapril & Enalaprilat Human Plasma 0.25 ng/mL LC-MS/MS Felodipine Human Plasma 25 pg/mL LC-MS/MS Isradapine Human Plasma 0.1 ng/mL LC-MS/MS Metoprolol Human Plasma 1 ng/mL LC-MS/MS Metoprolol / Hydroxymetoprolol Human Plasma 2.5 ng/mL HPLC/FL Metoprolol / Hydroxymetoprolol Human Urine 1000 ng/mL HPLC/FL Nifedipine / NPO Human Plasma 1 ng/mL LC-MS/MS Pindolol Human Plasma 1 ng/mL HPLC/UV Propafenone / N-Despropylpropafenone- / 5- Hydroxypropafenone Human Plasma 250 pg/mL LC-MS/MS Propranolol / 4-Hydroxypropranolol Human Plasma 2.5 ng/mL HPLC/FL Propranolol Glucuronide Human Urine 1 mcg/mL HPLC/FL Quinidine Human Plasma 10 ng/mL HPLC/UV Reserpine Human Plasma 25 pg/mL HPLC/FL Verapamil / Norverapamil Plasma 2.5 ng/mL HPLC/FL
Analyte Matrix LLOQ Unit Platform Alprazolam Human Plasma 0.5 ng/mL LC-MS/MS Alprazolam / α-Hydroxyalprazolam Dog Plasma 0.5 / 0.5 ng/mL LC-MS/MS Alprazolam / α-Hydroxyalprazolam Human Plasma 0.5 / 0.5 ng/mL LC-MS/MS Amitriptyline Human Plasma 200 pg/mL LC-MS/MS Aprepitant Human Plasma 10 ng/mL LC-MS/MS Atomoxetine Human Plasma 10 ng/mL LC-MS/MS Buspirone / 1-PP Dog Plasma 100 pg/mL LC-MS/MS Buspirone / 1-PP Human Plasma 100 pg/mL LC-MS/MS Butalbital Human Plasma 0.1 mcg/mL HPLC/UV Caffeine Human Plasma 0.05 mcg/mL HPLC/UV Carbamazepine Human Plasma 0.05 mcg/mL HPLC/UV Carisbamate Human Plasma 5 ng/mL LC-MS/MS Citalopram Plasma 100 pg/mL LC-MS/MS Clonazepam / 7-Aminoclonzepam Human Plasma 0.1 / 0.2 ng/mL LC-MS/MS Cyclobenzaprine Dog Plasma 0.1 ng/mL LC-MS/MS Cyclobenzaprine Human Plasma 0.2 ng/mL LC-MS/MS Doxepin / Nordoxepin Human Urine 0.25 / 0.20 ng/mL LC-MS/MS Femoxitine / N-Desmethylfemoxitine Human Plasma 5 ng/mL HPLC/FL Femoxitine / N-Desmethylfemoxitine Monkey Plasma 5 ng/mL HPLC/FL Haloperidol Human Plasma 25 pg/mL LC-MS/MS Hydroxymephenytoin, (R)-4- / Hydroxymephenytoin, (S)-4- Human Plasma 10 ng/mL LC-MS/MS Hydroxymephenytoin, (R)-4- / Hydroxymephenytoin, (S)-4- Human Saliva 10 ng/mL LC-MS/MS Hydroxymephenytoin, (R)-4- / Hydroxymephenytoin, (S)-4- Human Urine 0.1 mcg/mL LC-MS/MS Hydroxymidazolam, α- / Hydroxymidazolam, 4- Dog Plasma 2 / 2 ng/mL LC-MS/MS Hydroxymidazolam, α- / Hydroxymidazolam, 4- Rat Plasma 2 / 2 ng/mL LC-MS/MS Imipramine / Desipramine Human Plasma 0.1 ng/mL LC-MS/MS Lorazepam Human Plasma 0.2 ng/mL LC-MS/MS Memantine Human Plasma 0.5 ng/mL LC-MS/MS Memantine N-Glucuronide Human Plasma 0.2 ng/mL LC-MS/MS Mephenytoin, (R)- / Mephenytoin, (S)- Human Plasma 10 ng/mL LC-MS/MS Mephenytoin, (R)- / Mephenytoin, (S)- Human Saliva 10 ng/mL LC-MS/MS Mephenytoin, (R)- / Mephenytoin, (S)- Human Urine 0.1 mcg/mL LC-MS/MS Methocarbamol Human Plasma 0.1 mcg/mL HPLC/FL Methylphenidate Human Plasma 0.1 ng/mL LC-MS/MS Midazolam / 1-Hydroxymidazolam / 4- Hydroxymidazolam Human Urine 10 / 2 / 2 ng/mL LC-MS/MS Midazolam /α-Hydroxymidazolam Human Plasma 0.5 / 0.5 ng/mL LC-MS/MS Midazolam / α-Hydroxymidazolam Human Urine 20 / 0.5 ng/mL LC-MS/MS Molindone Human Plasma 0.25 ng/mL LC-MS/MS Olanzapine Human Plasma 0.1 ng/mL LC-MS/MS Ondansetron Human Plasma 0.5 ng/mL LC-MS/MS CNS Methods 13
CNS Methods 14 Analyte Matrix LLOQ Unit Platform Paroxetine Human Plasma 0.2 ng/mL LC-MS/MS Phenytoin Human Plasma 0.25 mcg/mL HPLC/UV Phenytoin Human Urine 1 mcg/mL HPLC/UV Phenytoin / Phenylhydantoin / Phenobarbitol (Free Assay) Human Urine 0.1 mcg/mL HPLC/UV Primidone Human Plasma 25 ng/mL LC-MS/MS Risperidone Human Plasma 0.1 ng/mL LC-MS/MS Ropinirole Human Plasma 20 pg/mL LC-MS/MS Ropinirole Mini-Pig Plasma 20 pg/mL LC-MS/MS Sumatriptan Human Plasma 0.5 ng/mL LC-MS/MS Temazepam Human Plasma 20 ng/mL LC-MS/MS Tizanidine Human Plasma 0.1 ng/mL LC-MS/MS Topiramate Plasma 10 ng/mL LC-MS/MS Valproic Acid Human Plasma 0.5 mcg/mL LC-MS/MS Venlafaxine / O-Desmethylvenlafaxine Human Plasma 2.5 ng/ml LC-MS/MS Zolpidem Human Plasma 0.5 ng/mL LC-MS/MS Zolmitriptan / N-Desmethylzolmitriptan Human Plasma 0.1 / 0.1 ng/mL LC-MS/MS Zonisamide Human Serum 50 ng/mL HPLC/UV
Cold Medication Methods 15 Analyte Matrix LLOQ Unit Platform Acetaminophen (APAP) Human Plasma 0.1 mcg/mL LC-MS/MS Acetylsalicylic Acid / Salicylic Acid Human Plasma 25 / 50 ng/mL HPLC/UV Brompheniramine / Chlorpheniramine / Pseudeophedrine Human Plasma 0.5 / 0.5 / 5 ng/mL LC-MS/MS Chlorpheniramine Human Plasma 0.5 ng/mL LC-MS/MS Dextromethorphan Human Plasma 50 pg/mL LC-MS/MS Dextromethorphan / 3-Hydroxymorphinan / 4-Methoxymorphinan Human Plasma 10 ng/mL HPLC/FL Dextromethorphan / 3-Hydroxymorphinan / 4-Methoxymorphinan Human Urine 0.25 mcg/mL HPLC/FL Dextrorphan Human Plasma 10 ng/mL HPLC/FL Dextrorphan Human Urine 250 ng/mL HPLC/FL Diphenydramine Human Plasma 1 ng/mL LC-MS/MS Fexofenadine Human Plasma 1 ng/mL LC-MS/MS Guaifenesin / Naproxen Human Plasma 10 / 250 ng/mL LC-MS/MS Ibuprofen Human Plasma 0.25 mcg/mL LC-MS/MS Naproxen Human Plasma 250 ng/mL LC-MS/MS Phenylephrine Human Plasma 0.5 ng/mL LC-MS/MS Phenylephrine Human Urine 5 ng/mL LC-MS/MS
Cytochrome P450 & DDI Methods 16 1A2 Cimetidine Human Plasma 50 ng/mL Inhibitor Ciprofloxacin Human Serum 10 ng/mL Inhibitor Phenacetin / Acetaminophen † in vitro Substrate Rifampin Human DBS 200 ng/mL Inducer Theophylline * Human Plasma 50 ng/mL Inhibitor 2A6 Coumarin / 7-Hydroxycoumarin † in vitro Substrate Rifampin Human DBS 200 ng/mL Inhibitor 2B6 Buproprion / Hydroxybupropion in vitro Substrate Efavirenz Human Plasma 50 ng/mL Substrate Efavirenz / 8-Hydroxyefavirenz in vitro Substrate 2C8 Amodiaquine / N-Desethylamodiaquine in vitro Substrate Gemfibrozil Human Plasma 0.5 mcg/mL Inhibitor Paclitaxel / 6α-Hydroxypaclitaxel in vitro Substrate 2C9 Amiodarone / Desethylamiodarone Human Plasma 10 ng/mL Inhibitor Carbamazepine Human Plasma 0.05 mcg/mL Inducer Cimetidine Human Plasma 50 ng/mL Inhibitor Diclofenac / 4'-Hydroxydiclofenac in vitro Substrate Phenytoin Human Plasma 0.25 mcg/mL Inducer Rifampin Human DBS 200 ng/mL Inducer Tolbutamide / 4'-Hydroxytolbutamide Human Plasma 0.25 / 0.5 ng/mL 2C19 Carbamazeipine Human Plasma 0.05 mcg/mL Inducer Cimetidine Human Plasma 50 ng/mL Inhibitor Esomeprazole Human Plasma 0.25 ng/mL Substrate Lansoprazole Human Plasma 0.25 ng/mL Substrate Mephenytoin, (S-) / 4'-Hydroxy-(S)-Mephenytoin † in vitro Substrate Omeprazole Human Plasma 5 ng/mL Inhibitor / Substrate Pantoprazole Human Plasma 10 ng/mL Substrate Phenytoin Human Plasma 0.25 mcg/mL Inducer
Cytochrome P450 & DDI Methods 17 2D6 Atomoxetine Human Plasma 10 ng/mL Substrate Dextromethorphan / Dextrorphan Human Plasma 0.05 / 10 ng/mL Substrate Dextromethorphan / Dextrorphan † in vitro Substrate Imipramine / Desipramine * Human Plasma 0.1 ng/mL Inhibitor Paroxetine Human Plasma 100 pg/mL Inhibitor Quinidine Human Plasma 10 ng/mL Inhibitor 2E1 Chlorzoxasone / 6-Hydroxyclorzoxasone † in vitro Substrate 2J2 Ebastine Human Plasma 10 ng/mL Substrate Ebastine in vitro Substrate 3A4 / 3A5 Atorvastatin / p- and o-Hydroxyatorvastatin Human Plasma 0.2 / 0.2 / 0.2 ng/mL Substrate Atorvastatin / o-Hydroxyatorvastatin in vitro Substrate Buspirone / 1-PP Human Plasma 100 pg/mL Substrate Carbamazeipine Human Plasma 0.05 mcg/mL Inducer Cimetidine Human Plasma 50 ng/mL Inhibitor Dexamethasone Human Plasma 0.25 ng/mL Inducer Diltiazem / N-Desmethyl- / Desacetyl- Human Plasma 4 / 2 / 1 ng/mL Inhibitor Felodipine Human Plasma 1/25/1900 pg/mL Substrate Imipramine / Desipramine Human Plasma 0.1 ng/mL Inhibitor Ketoconazole Human Plasma 50 ng/mL Inducer Midazolam / α-Hydroxymidazolam Human Plasma 0.5 / 0.5 ng/mL Substrate Midazolam / α-Hydroxymidazolam † in vitro Substrate Nifedipine in vitro Substrate Phenytoin Human Plasma 0.25 mcg/mL Inducer Rifampin * Human DBS 200 ng/mL Inducer Simvastatin / Simvastatin Hydroxy Acid * Human Plasma 50 / 50 pg/mL Inhibitor Testosterone / 6-β-Hydroxytestosterone † in vitro Substrate Venlafaxine / O-Desmethylvenlafaxine Human Plasma 2.5 ng/mL Inhibitor
Gastrointestinal & Metabolic Methods 18 Analyte Matrix LLOQ Unit Platform Allopurinol / Oxypurinol Human Plasma 50 / 100 ng/mL LC-MS/MS Atorvastatin / p- and o-Hydroxyatorvastatin Human Plasma 0.2 / 0.2 / 0.2 ng/mL LC-MS/MS Esomeprazole Human Plasma 0.25 ng/mL LC-MS/MS Fenofibric Acid Human Plasma 20 ng/mL LC-MS/MS Gemfibrozil Human Plasma 0.5 mcg/mL HPLC/UV Gliclazide Human Plasma 100 ng/mL HPLC/UV Metformin Human Plasma 20 ng/mL LC-MS/MS Methylprednisolone Human Plasma 2.5 ng/mL LC-MS/MS Lansoprazole Human Plasma 0.25 ng/mL LC-MS/MS Omeprazole Human Plasma 5 ng/mL LC-MS/MS Oxandrolone Human Plasma 0.25 ng/mL LC-MS/MS Paraxanthine Human Plasma 25 ng/mL HPLC/UV Simvastatin / Simvastatin Hydroxy Acid Human Plasma 50 / 50 pg/mL LC-MS/MS
Human Drug Abuse Liability Methods 19 Analyte Matrix LLOQ Unit Platform Alprazolam / α-Hydroxyalprazolam Human Plasma 0.5 ng/mL LC-MS/MS Buprenorphine / Norbuprenorphine Human Plasma 50 ng/mL LC-MS/MS Codeine Human Plasma 1 ng/mL LC-MS/MS Hydrocodone Human Plasma 0.1 ng/mL LC-MS/MS Hydromorphone (Anticoagulant: K2EDTA) Human Plasma 40 pg/mL LC-MS/MS Hydromorphone (Anticoagulant: Sodium Heparin) Human Plasma 75 pg/mL LC-MS/MS Lorazepam Human Plasma 0.2 ng/mL LC-MS/MS Midazolam / α-Hydroxymidazolam Human Plasma 0.5 ng/mL LC-MS/MS Methylphenidate Human Plasma 0.1 ng/mL LC-MS/MS Morphine / Morphine-3-β-D-Glucuronide / Morphine-6-β-D-Glucuronide Human Plasma 0.5 / 5 / 2 ng/mL LC-MS/MS Noroxycodone / Oxycodone Human Plasma 0.25 ng/mL LC-MS/MS Oxymorphone Human Plasma 0.25 ng/mL LC-MS/MS Zolpidem Human Plasma 0.5 ng/mL LC-MS/MS
Infectious Disease Methods 20 Analyte Matrix LLOQ Unit Platform Acyclovir Human Plasma 20 ng/mL HPLC/FL Acyclovir Human Urine 1 mcg/mL HPLC/FL Amikacin Human Serum 0.15 mcg/mL LC-MS/MS Amikacin Human Urine 0.5 mcg/mL LC-MS/MS Amoxicillin Plasma 0.25 mcg/mL HPLC/UV Ampicillin Plasma 0.25 mcg/mL HPLC/UV Azithromycin Human Plasma 2 ng/mL LC-MS/MS Cefaclor Plasma 0.5 mcg/mL HPLC/UV Cefadroxil Plasma 0.25 mcg/mL HPLC/UV Cefdinir Plasma 25 ng/mL HPLC/UV Cefixime Plasma 50 ng/mL HPLC/UV Cefprozil Plasma 0.25 mcg/mL HPLC/UV Ciprofloxacin Cat Plasma 25 ng/mL HPLC/FL Ciprofloxacin Human Serum 10 ng/mL LC-MS/MS Clarithromycin Human Plasma 10 ng/mL LC-MS/MS Clavulanic Acid Plasma 10 ng/mL HPLC/UV Clindamycin Plasma 100 ng/mL LC-MS/MS Clotrimazole / Ketoconazole Human Plasma 5 / 50 ng/mL LC-MS/MS Doxycycline Plasma 50 ng/mL HPLC/UV Erythromycin A Plasma 0.1 mcg/mL HPLC/EC Fluconazole Plasma 50 ng/mL HPLC/UV Efavirenz Human Plasma 50 ng/mL LC-MS/MS Ethambutol Human DBS 100 ng/mL LC/MS/MS Ibuprofen Human Plasma 0.25 mcg/mL LC-MS/MS Ketoconazole Human Plasma 50 ng/mL HPLC/FL Ketoconazole / Clotrimazole Human Plasma 50 / 5 ng/mL LC-MS/MS Levofloxacin Rabbit Plasma 0.2 mcg/mL LC-MS/MS Moxifloxacin Human DBS 100 ng/mL LC-MS/MS Moxifloxacin Human Plasma 20 ng/mL LC-MS/MS Naproxen Human Plasma 0.5 mcg/mL HPLC/FL Nitrofurantoin Human Plasma 5 ng/mL HPLC/UV Nitrofurantoin Human Urine 2 mcg/mL HPLC/UV Pyrazinamide Human DBS 10 mcg/mL LC-MS/MS Ribavirin Human Plasma 5 ng/mL LC-MS/MS Rifampin Human DBS 200 ng/mL LC-MS/MS Tenofovir Human Plasma 10 ng/mL LC-MS/MS Terbinafine Human Nail 200 mcg/g LC-MS/MS Terbinafine / N-Desmethylterbinafine Human Plasma 40 pg/mL LC-MS/MS
Oncology Methods 21 Analyte Matrix LLOQ Unit Platform Azacytidine, 5- Human Plasma 10 ng/mL LC-MS/MS Bicalutamide Human Plasma 2.5 ng/mL LC-MS/MS Cytarabine (Ara-C) Human Plasma 20 ng/mL LC-MS/MS Doxorubicin Human Urine 1.5 ng/mL LC-MS/MS Doxorubicin / Doxorubicinol Human Plasma 0.5 / 0.5 ng/mL LC-MS/MS Gemcitabine Human Plasma 40 ng/mL HPLC/UV Gemcitabine Human Urine 40 ng/mL HPLC/UV Melphalan Human Plasma 25 ng/mL LC-MS/MS Paclitaxel Human Urine 5 ng/mL LC-MS/MS Paclitaxel Human Plasma 100 pg/mL LC-MS/MS Paclitaxel Human Plasma 1 ng/mL LC-MS/MS Tretinoin Human Plasma 1 ng/mL LC-MS/MS

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Validated Method List

  • 1. Validated Method List New Methods & Methods Under Development KCAS Bioanalytical Services | kcasbio.com 1 New Methods Methods Under Development Analyte Matrix LLOQ Unit Platform Amlodipine Human Plasma 0.15 ng/mL LC-MS/MS Guaifenesin Human Plasma 10 ng/mL LC-MS/MS Methylphenidate Human Plasma 0.1 ng/mL LC-MS/MS Terbinafine Human Nail 200 mcg/g LC-MS/MS Zolmitriptan / N-Desmethylzolmitriptan Human Plasma 0.1 / 0.1 ng/mL LC-MS/MS Analyte Matrix LLOQ Unit Platform ACTH Intact 1-39 Human Plasma 7 pg/mL ELISA Amantadine Human Plasma 2 ng/mL LC-MS/MS L-Amphetamine / D-Amphetamine Human Plasma 0.2 / 0.25 ng/mL LC-MS/MS C-Reactive Protein Human Plasma 62.5 ng/mL ELISA Inflammatory Cytokine Panel: 4 to 9-plex Human Plasma Inquire ELISA Interferon b1 Human Plasma 2 pg/mL ELISA Interferon, gamma- Human Plasma 15 pg/mL ELISA Interleukin-6 (IL-6) Human Plasma & Serum 1.56 pg/mL ELISA PTH 1-34 Human Plasma 10 pg/mL ELISA PTH 1-84 Intact Human Plasma 10 pg/mL ELISA Testosterone Rat & Mini Pig Plasma 0.3 ng/mL LC-MS
  • 2. Validated Method List 2 Analyte Matrix LLOQ Unit Platform Acebutolol / Diacetolol Human Plasma 5 ng/mL HPLC/UV Acetaminophen (APAP) Human Plasma 0.1 mcg/mL LC-MS/MS Acetaminophen / Tramadol Human Plasma 100 / 5 ng/mL LC-MS/MS Acetylsalicylic Acid / Salicylic Acid Human Plasma 0.25/ 0.75 mcg/mL HPLC/UV Acid Glycoprotein, α1 Human Plasma 3.5 ng/mL ELISA Acyclovir Human Plasma 20 ng/mL HPLC/FL Acyclovir Human Urine 1 mcg/mL HPLC/FL Adipenectin Human Serum 0.8 ng/mL ELISA Alkaline Hematin (see Menstrual Blood) Menstrual Products Allopurinol / Oxypurinol Human Plasma 50 / 100 ng/mL LC-MS/MS Alprazolam / α-Hydroxyalprazolam Dog Plasma 0.5 / 0.5 ng/mL LC-MS/MS Alprazolam / α-Hydroxyalprazolam Human Plasma 0.5 / 0.5 ng/mL LC-MS/MS Aluminum Human Plasma 5 / 2 ng/mL HPLC Aluminum Human Urine 5 / 2 ng/mL HPLC Amikacin Human Serum 0.15 mcg/mL LC-MS/MS Amikacin Human Urine 0.5 mcg/mL LC-MS/MS Aminosalicylic Acid, 5- Human Feces 1 ng on col HPLC/FL Aminosalicylic Acid, 5- Human Urine 2 mcg/mL HPLC/FL Aminosalicylic Acid, 5- Rat Plasma 10 ng/mL LC-MS/MS Aminosalicylic Acid, 5- / N-Acetyl-5-Aminosalicylic Acid Human Plasma 10 / 10 ng/mL HPLC/FL Amiodarone / Desethylamiodarone Human Plasma 10 ng/mL LC-MS/MS Amitriptyline Human Plasma 200 pg/mL LC-MS/MS Amlodipine Human Plasma 50 pg/mL LC-MS/MS Amlodipine Human Plasma 0.15 ng/mL LC-MS/MS Anipamil Human Plasma 5 ng/mL HPLC/FL Aprepitant Human Plasma 10 ng/mL LC-MS/MS Aspirin (See Acetylsalicylic Acid) Human Plasma 0.25 ng/mL HPLC/UV Atenolol Human Plasma 10 ng/mL LC-MS/MS Atenolol Human Urine 250 ng/mL LC-MS/MS Atenolol, (R)- and (S)- Human Plasma 20 / 20 ng/mL HPLC/FL Atenolol, (R)- and (S)- Rat Plasma 20 / 20 ng/mL HPLC/FL Atomoxetine Human Plasma 10 ng/mL LC-MS/MS Atorvastatin / p- and o-Hydroxyatorvastatin Human Plasma 0.2 / 0.2 / 0.2 ng/mL LC-MS/MS Azacytidine, 5- Human Plasma 10 ng/mL LC-MS/MS Azithromycin Human Plasma 2 ng/mL LC-MS/MS Benazepril/ Benazeprilat Dog Plasma 0.1 / 0.5 ng/mL LC-MS/MS Benazepril / Benazeprilat Human Plasma 2 / 2 ng/mL LC-MS/MS Bicalutamide Human Plasma 2.5 ng/mL LC-MS/MS Bucladesine Human Plasma 1 ng/mL LC-MS/MS Brompheniramine / Chlorpheniramine / Pseudeophedrine Human Plasma 0.5 / 0.5 / 5 ng/mL LC-MS/MS Buprenorphine / Norbuprenorphine Dog Plasma 100 pg/mL LC-MS/MS
  • 3. Validated Method List 3 Analyte Matrix LLOQ Unit Platform Buprenorphine / Norbuprenorphine Human Plasma 50 pg/mL LC-MS/MS Buprenorphine / Norbuprenorphine Rabbit Plasma 50 pg/mL LC-MS/MS Buspirone / 1-PP Dog Plasma 100 pg/mL LC-MS/MS Buspirone / 1-PP Human Plasma 100 pg/mL LC-MS/MS Butalbital Human Plasma 0.1 mcg/mL HPLC/UV Butorphanol Human Plasma 20 pg/mL LC-MS/MS Caffeine Human Plasma 0.05 mcg/mL HPLC/UV Canrenone Human Plasma 2 ng/mL LC-MS/MS Canrenone Human Urine 20 ng/mL LC-MS/MS Captisol Human Plasma 5 mcg/mL LC-MS/MS Carbamazepine Human Plasma 0.05 mcg/mL HPLC/UV Carisbamate Human Plasma 5 ng/mL LC-MS/MS Cetirizine Human Plasma 2 ng/mL LC-MS/MS Chlorpheniramine Human Plasma 0.5 ng/mL LC-MS/MS Cimetidine Dog Plasma 50 ng/mL HPLC/UV Cimetidine Human Plasma 50 ng/mL HPLC/UV Ciprofloxacin Cat Plasma 25 ng/mL HPLC/FL Ciprofloxacin Human Serum 10 ng/mL LC-MS/MS Ciprofloxacin Human Plasma 10 ng/mL LC-MS/MS Cisapride Human Plasma 5 ng/mL HPLC/FL Citric Acid Human Urine 50 mcg/mL LC-MS/MS Clarithromycin Human Plasma 10 ng/mL LC-MS/MS Clonazepam / 7-Hydroxyclonazepam Human Plasma 0.1 / 0.2 pg/mL LC-MS/MS Clonidine Human Plasma 10 pg/mL LC-MS/MS Clotrimazole / Ketoconazole Human Plasma 5 / 50 ng/mL LC-MS/MS Cluster of Differentiation 14 (CD-14) Human Plasma 100 pg/mL ELISA Codeine Human Plasma 1 ng/mL LC-MS/MS Cordycepin Human Plasma 1 ng/mL LC-MS/MS Cortisol Human Plasma 40.4 ng/mL LC-MS/MS Cortisone Human Plasma 5 ng/mL HPLC/UV Cyclobenzaprine Dog Plasma 0.1 ng/mL LC-MS/MS Cyclobenzaprine Human Plasma 0.2 ng/mL LC-MS/MS Cytarabine (Ara-C) Human Plasma 20 ng/mL LC-MS/MS Deoxycholic Acid Human Plasma 10 ng/mL LC-MS/MS Dexamethasone Human Plasma 0.25 ng/mL LC-MS/MS Dextromethorphan Human Plasma 50 pg/mL LC-MS/MS Dextromethorphan / 3-Hydroxymorphinan / 4-Methoxymorphinan Human Plasma 10 ng/mL HPLC/FL Dextromethorphan / 3-Hydroxymorphinan / 4-Methoxymorphinan Human Urine 0.25 mcg/mL HPLC/FL Dextrorphan Human Plasma 10 ng/mL HPLC/FL Dextrorphan Human Urine 250 ng/mL HPLC/FL Diclofenac Human Plasma 5 ng/mL LC-MS/MS Diclofenac Rat Plasma 10 ng/mL HPLC/UV
  • 4. Validated Method List 4 Analyte Matrix LLOQ Unit Platform Digoxin Human Plasma 0.1 ng/mL LC-MS/MS Diltiazem / N-Desmethyldiltiazem / Desacetyldiltiazem Human Plasma 4 / 2 / 1 ng/mL LC-MS/MS Diphenydramine Human Plasma 1 ng/mL LC-MS/MS Dipyridamole Human Plasma 50 ng/mL LC-MS/MS DNA Quantitation Human Tissue 50 ng/mL Fluorometric Doxepin / Nordoxepin Human Urine 0.25 / 0.20 ng/mL LC-MS/MS Doxorubicin Human Urine 1.5 ng/mL LC-MS/MS Doxorubicin/Doxorubicinol Human Plasma 0.5 / 0.5 ng/mL LC-MS/MS Doxylamine Human Plasma 5 / 100 ng/mL HPLC/UV Doxylamine Human Urine 5 / 100 ng/mL HPLC/UV Dyclonine Dog Serum 1 ng/mL LC-MS/MS Ebastine Human Plasma 10 ng/mL HPLC/UV Efavirenz Human Plasma 50 ng/mL LC-MS/MS Enalapril / Enalaprilat Human Plasma 0.25 ng/mL LC-MS/MS Esomeprazole Human Plasma 0.25 ng/mL LC-MS/MS Ethambutol Human DBS 100 ng/mL LC-MS/MS Famotidine Human Plasma 2 ng/mL LC-MS/MS Famotidine Human Urine 2 ng/mL LC-MS/MS Felodipine Human Plasma 25 pg/mL LC-MS/MS Femoxitine / N-Desmethylfemoxitine Human Plasma 5 ng/mL HPLC/FL Femoxitine / N-Desmethylfemoxitine Monkey Plasma 5 ng/mL HPLC/FL Fenofibric Acid Human Plasma 20 ng/mL LC-MS/MS Fexofenadine Human Plasma 1 ng/mL LC-MS/MS Fexofenadine Mouse Plasma 25 ng/mL LC-MS/MS Fexofenadine Rabbit Plasma 25 ng/mL LC-MS/MS Fexofenadine Rat Plasma 25 ng/mL LC-MS/MS Flavoxate / MFCA metabolite Human Plasma 0.1 ng/mL LC-MS/MS Flurbiprofen Human Plasma 5 ng/mL LC-MS/MS Flurbiprofen, (R)- and (S)- Human Plasma 0.25 mcg/mL HPLC/FL Flurbiprofen, (R)- and (S)- Human Urine 0.5 mcg/mL HPLC/FL Furosemide Human Plasma 50 ng/mL LC-MS/MS Furosemide Human Urine 50 ng/mL LC-MS/MS Gemcitabine Human Plasma 40 ng/mL HPLC/UV Gemcitabine Human Urine 40 ng/mL HPLC/UV Gemfibrozil Human Plasma 0.5 mcg/mL HPLC/UV Ghrelin Active Human Plasma 100 pg/mL ELISA Ghrelin Total Human Plasma 200 pg/mL ELISA Gliclazide Human Plasma 100 ng/mL HPLC/UV Granisetron Dog Plasma 0.1 ng/mL LC-MS/MS Guaifenesin / Naproxen Human Plasma 10 / 250 ng/mL LC-MS/MS Haloperidol Human Plasma 25 pg/mL LC-MS/MS Hepatocyte Growth Factor (HGF) Human Plasma 400 pg/mL ELISA Heptazyme Antibodies Monkey Serum 1:25,600 titer ELISA
  • 5. Validated Method List 5 Analyte Matrix LLOQ Unit Platform Homocysteine Dog Plasma 5 mcg/mL LC-MS/MS Homocysteine Rat Plasma 5 mcg/mL LC-MS/MS Hydrochlorothiazide (HCTZ) Human Plasma 1 ng/mL HPLC/UV Hydrochlorothiazide (HCTZ) Human Urine 0.05 mcg/mL HPLC/UV Hydrocodone Human Plasma 0.1 ng/mL LC-MS/MS Hydromorphone Dog Plasma 20 pg/mL LC-MS/MS Hydromorphone Human Plasma 40 pg/mL LC-MS/MS Hydroxymephenytoin, (R)-4- / Hydroxymephenytoin, (S)-4- Human Plasma 10 ng/mL LC-MS/MS Hydroxymephenytoin, (R)-4- / Hydroxymephenytoin, (S)-4- Human Saliva 10 ng/mL LC-MS/MS Hydroxymephenytoin, (R)-4- / Hydroxymephenytoin, (S)-4- Human Urine 0.1 mcg/mL LC-MS/MS Hydroxymidazolam, α- / Hydroxymidazolam, 4- Dog Plasma 2 / 2 ng/mL LC-MS/MS Hydroxymidazolam, α- / Hydroxymidazolam, 4- Rat Plasma 2 / 2 ng/mL LC-MS/MS Ibuprofen Human Plasma 0.25 mcg/mL LC-MS/MS Ibuprofen, (R)- and (S)- Human Plasma 0.25 mcg/mL HPLC/UV Ibuprofen, (R)- and (S)- Human Urine 2.5 mcg/mL HPLC/FL Imipramine / Desipramine Human Plasma 0.1 ng/mL LC-MS/MS Indocyanine Green (ICG) Human Plasma 0.3 mcg/mL HPLC/UV Indomethacin Human Plasma 10 ng/mL LC-MS/MS Indomethacin Plasma/Serum 20 ng/mL HPLC/FL Interferon alpha Human Serum 10 pg/mL ELISA Isotretinoin / 4-Oxo-Isotretinoin Human Plasma 10 ng/mL LC-MS/MS Isradapine Human Plasma 0.1 ng/mL LC-MS/MS Ketoconazole / Clotrimazole Human Plasma 50 / 5 ng/mL LC-MS/MS Ketoprofen Human Plasma 0.25 ng/mL LC-MS/MS Lansoprazole Human Plasma 0.25 ng/mL LC-MS/MS Levofloxacin Rabbit Plasma 10 ng/mL LC-MS/MS Lorazepam Human Plasma 0.2 ng/mL LC-MS/MS Losartan / Carboxylosartan Human Plasma 2 / 2 ng/mL LC-MS/MS Meloxicam Human Plasma 10 ng/mL LC-MS/MS Melphalan Human Plasma 25 ng/mL LC-MS/MS Memantine Human Plasma 0.5 ng/mL LC-MS/MS Memantine N-Glucuronide Human Plasma 0.2 ng/mL LC-MS/MS Menstrual Blood (as Alkaline Hematin) Menstrual Products 1 mL Colorimetric Mephenytoin, (R)- / Mephenytoin, (S)- Human Plasma 10 ng/mL LC-MS/MS Mephenytoin, (R)- / Mephenytoin, (S)- Human Saliva 10 ng/mL LC-MS/MS Mephenytoin, (R)- / Mephenytoin, (S)- Human Urine 0.1 mcg/mL LC-MS/MS Mercaptopurine, 6- / 6-Thiouric Acid Human Plasma 2 / 20 ng/mL LC-MS/MS Mercaptopurine, 6- / 6-Thiouric Acid Human Urine 6 / 60 ng/mL LC-MS/MS Metformin Human Plasma 20 ng/mL LC-MS/MS Methocarbamol Human Plasma 0.1 mcg/mL HPLC/FL Methylnicotinamide Human Plasma 10 ng/mL LC-MS/MS
  • 6. Validated Method List 6 Analyte Matrix LLOQ Unit Platform Methylphenidate Human Plasma 0.1 ng/mL LC-MS/MS Methylprednisolone Human Plasma 2.5 ng/mL LC-MS/MS Metoclopramide Human Plasma 1 ng/mL HPLC/UV Metoclopramide Human Urine 60 ng/mL HPLC/FL Metoprolol Human Plasma 1 ng/mL LC-MS/MS Metoprolol / Hydroxymetoprolol Human Plasma 2.5 ng/mL HPLC/FL Metoprolol / Hydroxymetoprolol Human Urine 1000 ng/mL HPLC/FL Midazolam / α-Hydroxymidazolam Human Plasma 0.5 / 0.5 ng/mL LC-MS/MS Midazolam / 1-Hydroxymidazolam / 4-Hydroxymidazolam Human Urine 10 / 2 / 2 ng/mL LC-MS/MS Midazolam / α-Hydroxymidazolam Human Urine 20 / 0.5 ng/mL LC-MS/MS Molindone Human Plasma 0.25 ng/mL LC-MS/MS Monocyte Chemotactic Protein 1 (MCP-1) Human Plasma 30 pg/mL ELISA Monocyte Chemotactic Protein 3 (MCP-3) Human Plasma 15 pg/mL ELISA Morphine / Morphine-3-Glucuronide / Morphine-6-Glucuronide Dog Plasma 0.5 / 5 / 2 ng/mL LC-MS/MS Morphine / Morphine-3-Glucuronide / Morphine-6-Glucuronide Human Plasma 0.5 / 5 / 2 ng/mL LC-MS/MS Morphine / Morphine-3-Glucuronide / Morphine-6-Glucuronide Ovine Plasma 0.5 / 5 / 2 ng/mL LC-MS/MS Moxifloxacin Human DBS 100 ng/mL LC-MS/MS Moxifloxacin Human Plasma 20 ng/mL LC-MS/MS Naproxen Human Plasma 0.25 mcg/mL LC-MS/MS Niacin (Nicotinic Acid) / Nicotinuric Acid Human Urine 25 ng/mL LC-MS/MS Nifedipine / NPO Human Plasma 1 ng/mL LC-MS/MS Nitrofurantoin Human Plasma 5 ng/mL HPLC/UV Nitrofurantoin Human Urine 2 mcg/mL HPLC/UV Noroxycodone Human Plasma 0.25 ng/mL LC-MS/MS Olanzapine Human Plasma 0.1 ng/mL LC-MS/MS Omeprazole Human Plasma 5 ng/mL LC-MS/MS Ondansetron Human Plasma 0.5 ng/mL LC-MS/MS Oxandrolone Human Plasma 0.25 ng/mL LC-MS/MS Oxaprozin Human Plasma 2 mcg/mL HPLC/UV Oxybutynin / N-Desethyloxybutynin Human Plasma 50 / 250 pg/mL LC-MS/MS Oxycodone / Noroxycodcone / Oxymorphone Human Plasma 0.25 ng/mL LC-MS/MS Oxymorphone Human Plasma 0.25 ng/mL LC-MS/MS Paclitaxel Human Plasma 100 pg/mL LC-MS/MS Paclitaxel Human Urine 5 ng/mL LC-MS/MS Pantoprazole Human Plasma 10 ng/mL LC-MS/MS Paraxanthine Human Plasma 25 ng/mL HPLC/UV Paroxetine Human Plasma 0.2 ng/mL LC-MS/MS Pentazocine Human Plasma 1 ng/mL LC-MS/MS Phenylephrine Human Plasma 0.5 ng/mL LC-MS/MS Phenylephrine Human Urine 5 ng/mL LC-MS/MS
  • 7. Validated Method List 7 Analyte Matrix LLOQ Unit Platform Phenytoin Human Plasma 0.25 mcg/mL HPLC/UV Phenytoin Human Urine 1 mcg/mL HPLC/UV Phenytoin / Phenylhydantoin / Phenobarbitol (Free Assay) Human Urine 0.1 mcg/mL HPLC/UV Pindolol Human Plasma 1 ng/mL HPLC/UV Piroxicam Human Plasma 50 ng/mL HPLC/UV Potassium Human Urine 8 mM ICP-MS Prednisolone Human Plasma 1 ng/mL HPLC/UV Prednisone Human Plasma 1 ng/mL HPLC/UV Primidone Human Plasma 25 ng/mL LC-MS/MS Propafenone / N-Despropylpropafenone- / 5-Hydroxypropafenone Human Plasma 250 pg/mL LC-MS/MS Propofol Human Plasma 2.5 ng/mL HPLC/FL Propoxyphene/Norpropoxyphene Human Plasma 1 ng/mL LC-MS/MS Propranolol / 4-Hydroxypropranolol Human Plasma 2.5 ng/mL HPLC/FL Propranolol Glucuronide Human Urine 1 mcg/mL HPLC/FL Pseudoephedrine Human Plasma 5 ng/mL LC-MS/MS Pyrazinamide Human DBS 10 mcg/mL LC-MS/MS Quinidine Human Plasma 10 ng/mL HPLC/UV Ranitidine Human Plasma 5 ng/mL HPLC/UV Reserpine Human Plasma 25 pg/mL HPLC/FL Resistin Human Plasma 160 ng/mL ELISA Ribavirin Human Plasma 5 ng/mL LC-MS/MS Rifampin Human DBS 200 ng/mL LC-MS/MS Risperidone Human Plasma 0.1 ng/mL LC-MS/MS Ropinirole Human Plasma 20 pg/mL LC-MS/MS Ropinirole Mini-Pig Plasma 20 pg/mL LC-MS/MS Rosuvastatin Human Plasma 0.2 ng/mL LC-MS/MS Salicylic Acid Plasma 0.7 mcg/mL HPLC/FL Salicylic Acid Urine 9.2 mcg/mL HPLC/FL Salicyluric Acid Human Plasma 25 ng/mL HPLC/UV Salicyluric Acid Human Urine 2.5 mcg/mL HPLC/UV Simvastatin / Simvastatin Hydroxy Acid Human Plasma 50 / 50 pg/mL LC-MS/MS Spironolactone Human Plasma 0.5 ng/mL LC-MS/MS Sumatriptan Human Plasma 0.5 ng/mL LC-MS/MS Tacrolimus Rat DBS 5 ng/mL LC-MS/MS Tacrolimus Dog Whole Blood 0.1 ng/mL LC-MS/MS Tacrolimus Human Whole Blood 0.5 ng/mL LC-MS/MS Temazepam Human Plasma 20 ng/mL LC-MS/MS Tenofovir Human Plasma 10 ng/mL LC-MS/MS Tenoxicam Human Plasma 20 ng/mL HPLC/UV
  • 8. Validated Method List 8 Analyte Matrix LLOQ Unit Platform Terbinafine Human Nail 200 mcg/g LC-MS/MS Terbinafine / N-Desmethylterbinafine Human Plasma 40 pg/mL LC-MS/MS Testosterone (total) Human Serum 5 ng/dL RIA Theophylline Human Plasma 50 ng/mL HPLC/UV Theophylline Human Plasma 0.2 mcg/mL HPLC/UV Theophylline Human Serum 0.2 mcg/mL HPLC/UV Tizanidine Human Plasma 100 pg/mL LC-MS/MS Tolbutamide / Carboxytolbutamide / Hydroxytolbutamide Human Plasma 0.25 / 0.5 / 0.5 mcg/mL HPLC/UV Tolbutamide / Carboxytolbutamide / Hydroxytolbutamide Human Urine 0.25 / 0.5 / 0.5 mcg/mL HPLC/UV Tramadol Dog Plasma 2 ng/mL LC-MS/MS Tramadol / Acetaminophen Human Plasma 2.5 / 100 ng/mL LC-MS/MS Tretinoin / Isotretinoin Human Plasma 10 / 10 ng/mL LC-MS/MS Triamcinolone Human Plasma 20 pg/mL LC-MS/MS Triamterene Human Plasma 2 ng/mL LC-MS/MS Tyramine Human Plasma 1 ng/mL LC-MS/MS Valproic Acid Human Plasma 0.5 mcg/mL LC-MS/MS Venlafaxine / O-Desmethylvenlafaxine Human Plasma 2.5 ng/mL LC-MS/MS Vitamin B6 Human Plasma 50 ng/mL LC-MS/MS Vitamin E Human Plasma 0.5 mcg/mL HPLC/UV Warfarin, (R)- and (S)- Human Plasma 50 ng/mL HPLC/FL Xanthine Human Urine 1 mcg/mL HPLC/UV Xanthine Human Plasma 0.5 mcg/mL HPLC/UV Zolpidem Human Plasma 0.5 ng/mL LC-MS/MS Zolmitriptan / N-Desmethylzolmitriptan Human Plasma 0.1 / 0.1 ng/mL LC-MS/MS Zonisamide Human Serum 50 ng/mL HPLC/UV
  • 9. Analgesic Methods 9 Analyte Matrix LLOQ Unit Platform Acetaminophen (APAP) Human Plasma 0.1 mcg/mL LC-MS/MS Acetaminophen / Tramadol Human Plasma 100 / 5 ng/mL LC-MS/MS Acetylsalicylic Acid / Salicylic Acid Human Plasma 0.25 / 0.75 mcg/mL HPLC/UV Buprenorphine / Norbuprenorphine Dog Plasma 100 pg/mL LC-MS/MS Buprenorphine / Norbuprenorphine Human Plasma 50 pg/mL LC-MS/MS Buprenorphine / Norbuprenorphine Rabbit Plasma 50 pg/mL LC-MS/MS Butorphanol Human Plasma 20 pg/mL LC-MS/MS Codeine Human Plasma 1 ng/mL LC-MS/MS Diclofenac Human Plasma 5 ng/mL LC-MS/MS Flurbiprofen Human Plasma 5 ng/mL LC-MS/MS Flurbiprofen, (R)- and (S)- Human Plasma 0.25 mcg/mL HPLC/FL Flurbiprofen, (R)- and (S)- Human Urine 0.5 mcg/mL HPLC/FL Hydrocodone Human Plasma 0.1 ng/mL LC-MS/MS Hydromorphone Dog Plasma 20 pg/mL LC-MS/MS Hydromorphone Human Plasma 20 pg/mL LC-MS/MS Ibuprofen Human Plasma 0.25 mcg/mL LC-MS/MS Ibuprofen, (R)- and (S)- Human Plasma 0.25 mcg/mL HPLC/UV Ibuprofen, (R)- and (S)- Human Urine 2.5 mcg/mL HPLC/FL Indomethacin Human Plasma 10 ng/mL LC-MS/MS Ketoprofen Human Urine 1000 ng/mL HPLC Ketoprofen Human Plasma 0.25 ng/mL LC-MS/MS Mefenamic Acid Human Plasma 40 ng/mL LC-MS/MS Meloxicam Human Plasma 10 ng/mL LC-MS/MS Morphine / Morphine-3-Glucuronide / Morphine-6-Glucuronide Dog Plasma 0.5 / 5 / 2 ng/mL LC-MS/MS Morphine / Morphine-3-Glucuronide / Morphine-6-Glucuronide Human Plasma 0.5 / 5 / 2 ng/mL LC-MS/MS Morphine / Morphine-3-Glucuronide / Morphine-6-Glucuronide Ovine Plasma 0.5 / 5 / 2 ng/mL LC-MS/MS Naproxen Human Plasma 0.25 mcg/mL LC-MS/MS Oxaprozin Human Plasma 2 mcg/mL HPLC/UV Noroxycodone Human Plasma 0.25 ng/mL LC-MS/MS Oxycodone / Noroxycodcone / Oxymorphone Human Plasma 0.25 ng/mL LC-MS/MS Oxymorphone Human Plasma 0.25 ng/mL LC-MS/MS Pentazocine Human Plasma 1 ng/mL LC-MS/MS Piroxicam Human Plasma 50 ng/mL HPLC/UV Propoxyphene / Norpropoxyphene Human Plasma 1 ng/mL LC-MS/MS Salicylic Acid Plasma 0.7 mcg/mL HPLC/FL Salicylic Acid Urine 9.2 mcg/mL HPLC/FL Salicyluric Acid Human Plasma 25 ng/mL HPLC/UV Salicyluric Acid Human Urine 2.5 mcg/mL HPLC/UV Tenoxicam Human Plasma 20 ng/mL HPLC/UV Tramadol Dog Plasma 2 ng/mL LC-MS/MS Tramadol / Acetaminophen Human Plasma 2.5 / 100 ng/mL LC-MS/MS
  • 10. Animal Methods 10 Analyte Matrix LLOQ Unit Platform Alprazolam / α-Hydroxyalprazolam Dog Plasma 0.5 ng/mL LC-MS/MS Aminosalicylic Acid, 5- Rat Plasma 10 ng/mL LC-MS/MS Atenolol, (R)- and (S)- Rat Plasma 20 / 20 ng/mL HPLC/FL Benazepril/ Benazeprilat Dog Plasma 0.1 / 0.5 ng/mL LC-MS/MS Buprenorphine / Norbuprenorphine Dog Plasma 100 pg/mL LC-MS/MS Buprenorphine / Norbuprenorphine Rabbit Plasma 50 pg/mL LC-MS/MS Buspirone / 1-PP Dog Plasma 100 pg/mL LC-MS/MS Cimetidine Dog Plasma 50 ng/mL HPLC/UV Ciprofloxacin Cat Plasma 25 ng/mL HPLC/FL Cyclobenzaprine Dog Plasma 0.1 ng/mL LC-MS/MS Diclofenac Rat Plasma 10 ng/mL HPLC/UV Dyclonine Dog Serum 1 ng/mL LC-MS/MS Femoxitine / N-Desmethylfemoxitine Monkey Plasma 5 ng/mL HPLC/FL Fexofenadine Mouse Plasma 25 ng/mL LC-MS/MS Fexofenadine Rabbit Plasma 25 ng/mL LC-MS/MS Fexofenadine Rat Plasma 25 ng/mL LC-MS/MS Granisetron Dog Plasma 0.1 ng/mL LC-MS/MS Homocysteine Dog Plasma 5 mcg/mL LC-MS/MS Homocysteine Rat Plasma 5 mcg/mL LC-MS/MS Hydromorphone Dog Plasma 20 pg/mL LC-MS/MS Hydromorphone Dog Serum 20 pg/mL LC-MS/MS Hydroxymidazolam, α- / Hydroxymidazolam, 4- Dog Plasma 2 / 2 ng/mL LC-MS/MS Hydroxymidazolam, α- / Hydroxymidazolam, 4- Rat Plasma 2 / 2 ng/mL LC-MS/MS Levofloxacin Rabbit Plasma 10 ng/mL LC-MS/MS Lidocaine Dog Plasma 0.1 ng/mL LC-MS/MS Lidocaine Dog Urine 0.1 ng/mL LC-MS/MS Lidocaine Dog Tissues 10 ng/g LC-MS/MS Lidocaine Rat Plasma 0.5 ng/mL LC-MS/MS Morphine / Morphine-3-Glucuronide / Morphine- 6-Glucuronide Dog Plasma 0.5 / 5 / 2 ng/mL LC-MS/MS Morphine / Morphine-3-Glucuronide / Morphine- 6-Glucuronide Ovine Plasma 0.5 / 5 / 2 ng/mL LC-MS/MS Ropinirole Mini-Pig Plasma 20 pg/mL LC-MS/MS Tacrolimus Dog Whole Blood 0.1 ng/mL LC-MS/MS Tramadol Dog Plasma 2 ng/mL LC-MS/MS
  • 11. Biomarkers 11 Analyte Matrix LLOQ Unit Platform Acid Glycoprotein, α1 Human Plasma 3.5 ng/mL ELISA Adipenectin Human Serum 0.8 ng/mL ELISA Cluster of Differentiation 14 (CD-14) Human Plasma 100 pg/mL ELISA Cortisol Human Plasma 40.4 ng/mL LC-MS/MS Cortisone Human Plasma 5 ng/mL HPLC/UV Deoxycholic Acid Human Plasma 10 ng/mL LC-MS/MS Ghrelin Human Serum 94 pg/mL ELISA Ghrelin Active Human Plasma 100 pg/mL ELISA Ghrelin Total Human Plasma 200 pg/mL ELISA Hepatocyte Growth Factor (HGF) Human Plasma 400 pg/mL ELISA Homocysteine Dog Plasma 5 mcg/mL LC-MS/MS Homocysteine Rat Plasma 5 mcg/mL LC-MS/MS Monocyte Chemotactic Protein 1 (MCP-1) Human Plasma 30 pg/mL ELISA Monocyte Chemotactic Protein 3 (MCP-3) Human Plasma 15 pg/mL ELISA Methylnicotinamide Human Plasma 10 ng/mL LC-MS/MS Resistin Human Plasma 160 ng/mL ELISA Tyramine Human Plasma 1 ng/mL LC-MS/MS Vitamin B6 Human Plasma 50 ng/mL LC-MS/MS Vitamin E Human Plasma 0.5 mcg/mL HPLC/UV Xanthine Human Plasma 0.5 mcg/mL HPLC/UV Xanthine Human Urine 1 mcg/mL HPLC/UV
  • 12. Cardiovascular Methods 12 Analyte Matrix LLOQ Unit Platform Acebutolol / Diacetolol Human Plasma 5 ng/mL HPLC/UV Amiodarone / Desethylamiodarone Human Plasma 10 ng/mL LC-MS/MS Amlodipine Human Plasma 50 pg/mL LC-MS/MS Anipamil Human Plasma 5 ng/mL HPLC/FL Atenolol Human Plasma 10 ng/mL LC-MS/MS Atenolol Human Urine 250 ng/mL LC-MS/MS Atenolol, (R)- and (S)- Human Plasma 20 ng/mL HPLC/FL Atenolol, (R)- and (S)- Rat Plasma 20 ng/mL HPLC/FL Benazepril / Benazeprilat Dog Plasma 0.1 / 0.5 ng/mL LC-MS/MS Benazepril / Benazeprilat Human Plasma 2 / 2 ng/mL LC-MS/MS Bucladesine Human Plasma 1 ng/mL LC-MS/MS Digoxin Human Plasma 0.1 ng/mL LC-MS/MS Diltiazem / N-Desmethyldiltiazem / Desacetyldiltiazem Human Plasma 4 / 2 / 1 ng/mL LC-MS/MS Dipyridamole Human Plasma 50 ng/mL LC-MS/MS Enalapril & Enalaprilat Human Plasma 0.25 ng/mL LC-MS/MS Felodipine Human Plasma 25 pg/mL LC-MS/MS Isradapine Human Plasma 0.1 ng/mL LC-MS/MS Metoprolol Human Plasma 1 ng/mL LC-MS/MS Metoprolol / Hydroxymetoprolol Human Plasma 2.5 ng/mL HPLC/FL Metoprolol / Hydroxymetoprolol Human Urine 1000 ng/mL HPLC/FL Nifedipine / NPO Human Plasma 1 ng/mL LC-MS/MS Pindolol Human Plasma 1 ng/mL HPLC/UV Propafenone / N-Despropylpropafenone- / 5- Hydroxypropafenone Human Plasma 250 pg/mL LC-MS/MS Propranolol / 4-Hydroxypropranolol Human Plasma 2.5 ng/mL HPLC/FL Propranolol Glucuronide Human Urine 1 mcg/mL HPLC/FL Quinidine Human Plasma 10 ng/mL HPLC/UV Reserpine Human Plasma 25 pg/mL HPLC/FL Verapamil / Norverapamil Plasma 2.5 ng/mL HPLC/FL
  • 13. Analyte Matrix LLOQ Unit Platform Alprazolam Human Plasma 0.5 ng/mL LC-MS/MS Alprazolam / α-Hydroxyalprazolam Dog Plasma 0.5 / 0.5 ng/mL LC-MS/MS Alprazolam / α-Hydroxyalprazolam Human Plasma 0.5 / 0.5 ng/mL LC-MS/MS Amitriptyline Human Plasma 200 pg/mL LC-MS/MS Aprepitant Human Plasma 10 ng/mL LC-MS/MS Atomoxetine Human Plasma 10 ng/mL LC-MS/MS Buspirone / 1-PP Dog Plasma 100 pg/mL LC-MS/MS Buspirone / 1-PP Human Plasma 100 pg/mL LC-MS/MS Butalbital Human Plasma 0.1 mcg/mL HPLC/UV Caffeine Human Plasma 0.05 mcg/mL HPLC/UV Carbamazepine Human Plasma 0.05 mcg/mL HPLC/UV Carisbamate Human Plasma 5 ng/mL LC-MS/MS Citalopram Plasma 100 pg/mL LC-MS/MS Clonazepam / 7-Aminoclonzepam Human Plasma 0.1 / 0.2 ng/mL LC-MS/MS Cyclobenzaprine Dog Plasma 0.1 ng/mL LC-MS/MS Cyclobenzaprine Human Plasma 0.2 ng/mL LC-MS/MS Doxepin / Nordoxepin Human Urine 0.25 / 0.20 ng/mL LC-MS/MS Femoxitine / N-Desmethylfemoxitine Human Plasma 5 ng/mL HPLC/FL Femoxitine / N-Desmethylfemoxitine Monkey Plasma 5 ng/mL HPLC/FL Haloperidol Human Plasma 25 pg/mL LC-MS/MS Hydroxymephenytoin, (R)-4- / Hydroxymephenytoin, (S)-4- Human Plasma 10 ng/mL LC-MS/MS Hydroxymephenytoin, (R)-4- / Hydroxymephenytoin, (S)-4- Human Saliva 10 ng/mL LC-MS/MS Hydroxymephenytoin, (R)-4- / Hydroxymephenytoin, (S)-4- Human Urine 0.1 mcg/mL LC-MS/MS Hydroxymidazolam, α- / Hydroxymidazolam, 4- Dog Plasma 2 / 2 ng/mL LC-MS/MS Hydroxymidazolam, α- / Hydroxymidazolam, 4- Rat Plasma 2 / 2 ng/mL LC-MS/MS Imipramine / Desipramine Human Plasma 0.1 ng/mL LC-MS/MS Lorazepam Human Plasma 0.2 ng/mL LC-MS/MS Memantine Human Plasma 0.5 ng/mL LC-MS/MS Memantine N-Glucuronide Human Plasma 0.2 ng/mL LC-MS/MS Mephenytoin, (R)- / Mephenytoin, (S)- Human Plasma 10 ng/mL LC-MS/MS Mephenytoin, (R)- / Mephenytoin, (S)- Human Saliva 10 ng/mL LC-MS/MS Mephenytoin, (R)- / Mephenytoin, (S)- Human Urine 0.1 mcg/mL LC-MS/MS Methocarbamol Human Plasma 0.1 mcg/mL HPLC/FL Methylphenidate Human Plasma 0.1 ng/mL LC-MS/MS Midazolam / 1-Hydroxymidazolam / 4- Hydroxymidazolam Human Urine 10 / 2 / 2 ng/mL LC-MS/MS Midazolam /α-Hydroxymidazolam Human Plasma 0.5 / 0.5 ng/mL LC-MS/MS Midazolam / α-Hydroxymidazolam Human Urine 20 / 0.5 ng/mL LC-MS/MS Molindone Human Plasma 0.25 ng/mL LC-MS/MS Olanzapine Human Plasma 0.1 ng/mL LC-MS/MS Ondansetron Human Plasma 0.5 ng/mL LC-MS/MS CNS Methods 13
  • 14. CNS Methods 14 Analyte Matrix LLOQ Unit Platform Paroxetine Human Plasma 0.2 ng/mL LC-MS/MS Phenytoin Human Plasma 0.25 mcg/mL HPLC/UV Phenytoin Human Urine 1 mcg/mL HPLC/UV Phenytoin / Phenylhydantoin / Phenobarbitol (Free Assay) Human Urine 0.1 mcg/mL HPLC/UV Primidone Human Plasma 25 ng/mL LC-MS/MS Risperidone Human Plasma 0.1 ng/mL LC-MS/MS Ropinirole Human Plasma 20 pg/mL LC-MS/MS Ropinirole Mini-Pig Plasma 20 pg/mL LC-MS/MS Sumatriptan Human Plasma 0.5 ng/mL LC-MS/MS Temazepam Human Plasma 20 ng/mL LC-MS/MS Tizanidine Human Plasma 0.1 ng/mL LC-MS/MS Topiramate Plasma 10 ng/mL LC-MS/MS Valproic Acid Human Plasma 0.5 mcg/mL LC-MS/MS Venlafaxine / O-Desmethylvenlafaxine Human Plasma 2.5 ng/ml LC-MS/MS Zolpidem Human Plasma 0.5 ng/mL LC-MS/MS Zolmitriptan / N-Desmethylzolmitriptan Human Plasma 0.1 / 0.1 ng/mL LC-MS/MS Zonisamide Human Serum 50 ng/mL HPLC/UV
  • 15. Cold Medication Methods 15 Analyte Matrix LLOQ Unit Platform Acetaminophen (APAP) Human Plasma 0.1 mcg/mL LC-MS/MS Acetylsalicylic Acid / Salicylic Acid Human Plasma 25 / 50 ng/mL HPLC/UV Brompheniramine / Chlorpheniramine / Pseudeophedrine Human Plasma 0.5 / 0.5 / 5 ng/mL LC-MS/MS Chlorpheniramine Human Plasma 0.5 ng/mL LC-MS/MS Dextromethorphan Human Plasma 50 pg/mL LC-MS/MS Dextromethorphan / 3-Hydroxymorphinan / 4-Methoxymorphinan Human Plasma 10 ng/mL HPLC/FL Dextromethorphan / 3-Hydroxymorphinan / 4-Methoxymorphinan Human Urine 0.25 mcg/mL HPLC/FL Dextrorphan Human Plasma 10 ng/mL HPLC/FL Dextrorphan Human Urine 250 ng/mL HPLC/FL Diphenydramine Human Plasma 1 ng/mL LC-MS/MS Fexofenadine Human Plasma 1 ng/mL LC-MS/MS Guaifenesin / Naproxen Human Plasma 10 / 250 ng/mL LC-MS/MS Ibuprofen Human Plasma 0.25 mcg/mL LC-MS/MS Naproxen Human Plasma 250 ng/mL LC-MS/MS Phenylephrine Human Plasma 0.5 ng/mL LC-MS/MS Phenylephrine Human Urine 5 ng/mL LC-MS/MS
  • 16. Cytochrome P450 & DDI Methods 16 1A2 Cimetidine Human Plasma 50 ng/mL Inhibitor Ciprofloxacin Human Serum 10 ng/mL Inhibitor Phenacetin / Acetaminophen † in vitro Substrate Rifampin Human DBS 200 ng/mL Inducer Theophylline * Human Plasma 50 ng/mL Inhibitor 2A6 Coumarin / 7-Hydroxycoumarin † in vitro Substrate Rifampin Human DBS 200 ng/mL Inhibitor 2B6 Buproprion / Hydroxybupropion in vitro Substrate Efavirenz Human Plasma 50 ng/mL Substrate Efavirenz / 8-Hydroxyefavirenz in vitro Substrate 2C8 Amodiaquine / N-Desethylamodiaquine in vitro Substrate Gemfibrozil Human Plasma 0.5 mcg/mL Inhibitor Paclitaxel / 6α-Hydroxypaclitaxel in vitro Substrate 2C9 Amiodarone / Desethylamiodarone Human Plasma 10 ng/mL Inhibitor Carbamazepine Human Plasma 0.05 mcg/mL Inducer Cimetidine Human Plasma 50 ng/mL Inhibitor Diclofenac / 4'-Hydroxydiclofenac in vitro Substrate Phenytoin Human Plasma 0.25 mcg/mL Inducer Rifampin Human DBS 200 ng/mL Inducer Tolbutamide / 4'-Hydroxytolbutamide Human Plasma 0.25 / 0.5 ng/mL 2C19 Carbamazeipine Human Plasma 0.05 mcg/mL Inducer Cimetidine Human Plasma 50 ng/mL Inhibitor Esomeprazole Human Plasma 0.25 ng/mL Substrate Lansoprazole Human Plasma 0.25 ng/mL Substrate Mephenytoin, (S-) / 4'-Hydroxy-(S)-Mephenytoin † in vitro Substrate Omeprazole Human Plasma 5 ng/mL Inhibitor / Substrate Pantoprazole Human Plasma 10 ng/mL Substrate Phenytoin Human Plasma 0.25 mcg/mL Inducer
  • 17. Cytochrome P450 & DDI Methods 17 2D6 Atomoxetine Human Plasma 10 ng/mL Substrate Dextromethorphan / Dextrorphan Human Plasma 0.05 / 10 ng/mL Substrate Dextromethorphan / Dextrorphan † in vitro Substrate Imipramine / Desipramine * Human Plasma 0.1 ng/mL Inhibitor Paroxetine Human Plasma 100 pg/mL Inhibitor Quinidine Human Plasma 10 ng/mL Inhibitor 2E1 Chlorzoxasone / 6-Hydroxyclorzoxasone † in vitro Substrate 2J2 Ebastine Human Plasma 10 ng/mL Substrate Ebastine in vitro Substrate 3A4 / 3A5 Atorvastatin / p- and o-Hydroxyatorvastatin Human Plasma 0.2 / 0.2 / 0.2 ng/mL Substrate Atorvastatin / o-Hydroxyatorvastatin in vitro Substrate Buspirone / 1-PP Human Plasma 100 pg/mL Substrate Carbamazeipine Human Plasma 0.05 mcg/mL Inducer Cimetidine Human Plasma 50 ng/mL Inhibitor Dexamethasone Human Plasma 0.25 ng/mL Inducer Diltiazem / N-Desmethyl- / Desacetyl- Human Plasma 4 / 2 / 1 ng/mL Inhibitor Felodipine Human Plasma 1/25/1900 pg/mL Substrate Imipramine / Desipramine Human Plasma 0.1 ng/mL Inhibitor Ketoconazole Human Plasma 50 ng/mL Inducer Midazolam / α-Hydroxymidazolam Human Plasma 0.5 / 0.5 ng/mL Substrate Midazolam / α-Hydroxymidazolam † in vitro Substrate Nifedipine in vitro Substrate Phenytoin Human Plasma 0.25 mcg/mL Inducer Rifampin * Human DBS 200 ng/mL Inducer Simvastatin / Simvastatin Hydroxy Acid * Human Plasma 50 / 50 pg/mL Inhibitor Testosterone / 6-β-Hydroxytestosterone † in vitro Substrate Venlafaxine / O-Desmethylvenlafaxine Human Plasma 2.5 ng/mL Inhibitor
  • 18. Gastrointestinal & Metabolic Methods 18 Analyte Matrix LLOQ Unit Platform Allopurinol / Oxypurinol Human Plasma 50 / 100 ng/mL LC-MS/MS Atorvastatin / p- and o-Hydroxyatorvastatin Human Plasma 0.2 / 0.2 / 0.2 ng/mL LC-MS/MS Esomeprazole Human Plasma 0.25 ng/mL LC-MS/MS Fenofibric Acid Human Plasma 20 ng/mL LC-MS/MS Gemfibrozil Human Plasma 0.5 mcg/mL HPLC/UV Gliclazide Human Plasma 100 ng/mL HPLC/UV Metformin Human Plasma 20 ng/mL LC-MS/MS Methylprednisolone Human Plasma 2.5 ng/mL LC-MS/MS Lansoprazole Human Plasma 0.25 ng/mL LC-MS/MS Omeprazole Human Plasma 5 ng/mL LC-MS/MS Oxandrolone Human Plasma 0.25 ng/mL LC-MS/MS Paraxanthine Human Plasma 25 ng/mL HPLC/UV Simvastatin / Simvastatin Hydroxy Acid Human Plasma 50 / 50 pg/mL LC-MS/MS
  • 19. Human Drug Abuse Liability Methods 19 Analyte Matrix LLOQ Unit Platform Alprazolam / α-Hydroxyalprazolam Human Plasma 0.5 ng/mL LC-MS/MS Buprenorphine / Norbuprenorphine Human Plasma 50 ng/mL LC-MS/MS Codeine Human Plasma 1 ng/mL LC-MS/MS Hydrocodone Human Plasma 0.1 ng/mL LC-MS/MS Hydromorphone (Anticoagulant: K2EDTA) Human Plasma 40 pg/mL LC-MS/MS Hydromorphone (Anticoagulant: Sodium Heparin) Human Plasma 75 pg/mL LC-MS/MS Lorazepam Human Plasma 0.2 ng/mL LC-MS/MS Midazolam / α-Hydroxymidazolam Human Plasma 0.5 ng/mL LC-MS/MS Methylphenidate Human Plasma 0.1 ng/mL LC-MS/MS Morphine / Morphine-3-β-D-Glucuronide / Morphine-6-β-D-Glucuronide Human Plasma 0.5 / 5 / 2 ng/mL LC-MS/MS Noroxycodone / Oxycodone Human Plasma 0.25 ng/mL LC-MS/MS Oxymorphone Human Plasma 0.25 ng/mL LC-MS/MS Zolpidem Human Plasma 0.5 ng/mL LC-MS/MS
  • 20. Infectious Disease Methods 20 Analyte Matrix LLOQ Unit Platform Acyclovir Human Plasma 20 ng/mL HPLC/FL Acyclovir Human Urine 1 mcg/mL HPLC/FL Amikacin Human Serum 0.15 mcg/mL LC-MS/MS Amikacin Human Urine 0.5 mcg/mL LC-MS/MS Amoxicillin Plasma 0.25 mcg/mL HPLC/UV Ampicillin Plasma 0.25 mcg/mL HPLC/UV Azithromycin Human Plasma 2 ng/mL LC-MS/MS Cefaclor Plasma 0.5 mcg/mL HPLC/UV Cefadroxil Plasma 0.25 mcg/mL HPLC/UV Cefdinir Plasma 25 ng/mL HPLC/UV Cefixime Plasma 50 ng/mL HPLC/UV Cefprozil Plasma 0.25 mcg/mL HPLC/UV Ciprofloxacin Cat Plasma 25 ng/mL HPLC/FL Ciprofloxacin Human Serum 10 ng/mL LC-MS/MS Clarithromycin Human Plasma 10 ng/mL LC-MS/MS Clavulanic Acid Plasma 10 ng/mL HPLC/UV Clindamycin Plasma 100 ng/mL LC-MS/MS Clotrimazole / Ketoconazole Human Plasma 5 / 50 ng/mL LC-MS/MS Doxycycline Plasma 50 ng/mL HPLC/UV Erythromycin A Plasma 0.1 mcg/mL HPLC/EC Fluconazole Plasma 50 ng/mL HPLC/UV Efavirenz Human Plasma 50 ng/mL LC-MS/MS Ethambutol Human DBS 100 ng/mL LC/MS/MS Ibuprofen Human Plasma 0.25 mcg/mL LC-MS/MS Ketoconazole Human Plasma 50 ng/mL HPLC/FL Ketoconazole / Clotrimazole Human Plasma 50 / 5 ng/mL LC-MS/MS Levofloxacin Rabbit Plasma 0.2 mcg/mL LC-MS/MS Moxifloxacin Human DBS 100 ng/mL LC-MS/MS Moxifloxacin Human Plasma 20 ng/mL LC-MS/MS Naproxen Human Plasma 0.5 mcg/mL HPLC/FL Nitrofurantoin Human Plasma 5 ng/mL HPLC/UV Nitrofurantoin Human Urine 2 mcg/mL HPLC/UV Pyrazinamide Human DBS 10 mcg/mL LC-MS/MS Ribavirin Human Plasma 5 ng/mL LC-MS/MS Rifampin Human DBS 200 ng/mL LC-MS/MS Tenofovir Human Plasma 10 ng/mL LC-MS/MS Terbinafine Human Nail 200 mcg/g LC-MS/MS Terbinafine / N-Desmethylterbinafine Human Plasma 40 pg/mL LC-MS/MS
  • 21. Oncology Methods 21 Analyte Matrix LLOQ Unit Platform Azacytidine, 5- Human Plasma 10 ng/mL LC-MS/MS Bicalutamide Human Plasma 2.5 ng/mL LC-MS/MS Cytarabine (Ara-C) Human Plasma 20 ng/mL LC-MS/MS Doxorubicin Human Urine 1.5 ng/mL LC-MS/MS Doxorubicin / Doxorubicinol Human Plasma 0.5 / 0.5 ng/mL LC-MS/MS Gemcitabine Human Plasma 40 ng/mL HPLC/UV Gemcitabine Human Urine 40 ng/mL HPLC/UV Melphalan Human Plasma 25 ng/mL LC-MS/MS Paclitaxel Human Urine 5 ng/mL LC-MS/MS Paclitaxel Human Plasma 100 pg/mL LC-MS/MS Paclitaxel Human Plasma 1 ng/mL LC-MS/MS Tretinoin Human Plasma 1 ng/mL LC-MS/MS