What's the right quaternary LC system for your analysis?
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The document discusses the cost considerations and ownership of various analytical liquid chromatography (LC) systems, focusing on the differences between HPLC, UHPLC, and UPLC technologies. Waters’ portfolio aims to provide reliable and efficient solutions for laboratories, with an emphasis on maximizing efficiency and scalability through correct system pairing. It highlights the importance of optimizing column dimensions, flow rates, and operational pressures for improved separation performance.
![[ ANALYTICAL LC PORTFOLIO ]
®2015WatersCorporation.June2015720005426ENLM-PDF
CONSIDER THE ENTIRE COST OF YOUR ASSETS
Consider your cost of ownership… …by considering your cost of analysis.
Initial Cost
Time
Total Cost of Ownership
HPLC
UHPLC
UPLC
CostofSolvent
CostofOwnership
Time
HPLC
UHPLC
UPLC
9x more
than UPLC
4x more
than UPLC
What’s the right quaternary
LC for your analysis?
WATERS’ PORTFOLIO OF ANALYTICAL LC SYSTEMS IS DESIGNED
TO MEET YOUR REQUIREMENTS FOR RELIABILITY,
ROBUSTNESS, AND REPEATABILITY
The time-tested, reliable
workhorse for your HPLC
separations – that isn’t
going away any time soon.
A versatile LC platform for
laboratories in need of a
solution to replicate or
improve HPLC methods.
Run true UPLC
separations and
maximize the efficiency
of your laboratory.
SYSTEM SCALABILILTY: MATCHING LC COLUMNS WITH THE RIGHT TECHNOLOGY
AU
0.000
0.010
0.020
0.030
0.040
0.050
0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60
3.75
AU
0.000
0.010
0.020
0.030
0.040
0.050
2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00
15.00
XBridge C18 5 µm, 4.6 x 150 mm
Flow rate = 1.0 mL/min
Solvent consumed = 15 mL/injection
XBridge C18 2.5 µm, 3.0 x 75 mm
Flow rate = 0.85 mL/min
Solvent consumed = 3.2 mL/injection
ACQUITY BEH C18 1.7 µm, 2.1 x 50 mm
Flow rate = 0.61 mL/min
Solvent consumed = 1.0 mL/injection
Alliance
ACQUITY Arc
ACQUITY UPLC
H-Class
AU
0.000
0.010
0.020
0.030
0.040
0.050
min
min
min
0.30 0.40 0.50 0.60 0.70 0.80 0.90 1.00 1.10 1.20 1.30 1.40 1.50 1.60
1.70
Rs = 2.0
Rs = 2.0
Rs = 2.0
PcAve.
W@50% 122
PcAve.
W@50% 118
PcAve.
W@50% 116
DISPERSION MATTERS: RETHINKING LC SEPARATION CATEGORIES
HPLC
UHPLC
UPLC
HPLC
<6000 psi
UHPLC
6000 –
15,000 psi
UPLC
9000–
15,000 psi
UPLC
typically
<1 mL/min
DISPERSION IMPACT
OPTIMAL PARTICLE SIZE COST PER SAMPLE OPTIMAL COLUMN I.D.
TYPICAL OPERATING PRESSURE FLOW RATE
HPLC
typically
<3 mL/min
HPLC
≥3.5 µm
UHPLC
1.5–5 µm
UPLC
<2 µm
HPLC UHPLC UPLC
HPLC
≥4.6 mm
UHPLC
2.1– 4.6 mm
UPLC
1–3 mm
UHPLC
typically
<2 mL/min
www.waters.com/lcportfolio
For the best system performance, pair the right system dispersion and characteristics with the right column.](https://image.slidesharecdn.com/5426iglcportfolioinfographicwebfinal-160201175904/85/What-s-the-right-quaternary-LC-system-for-your-analysis-1-320.jpg)
What's the right quaternary LC system for your analysis?
- 1. [ ANALYTICAL LC PORTFOLIO ] ®2015WatersCorporation.June2015720005426ENLM-PDF CONSIDER THE ENTIRE COST OF YOUR ASSETS Consider your cost of ownership… …by considering your cost of analysis. Initial Cost Time Total Cost of Ownership HPLC UHPLC UPLC CostofSolvent CostofOwnership Time HPLC UHPLC UPLC 9x more than UPLC 4x more than UPLC What’s the right quaternary LC for your analysis? WATERS’ PORTFOLIO OF ANALYTICAL LC SYSTEMS IS DESIGNED TO MEET YOUR REQUIREMENTS FOR RELIABILITY, ROBUSTNESS, AND REPEATABILITY The time-tested, reliable workhorse for your HPLC separations – that isn’t going away any time soon. A versatile LC platform for laboratories in need of a solution to replicate or improve HPLC methods. Run true UPLC separations and maximize the efficiency of your laboratory. SYSTEM SCALABILILTY: MATCHING LC COLUMNS WITH THE RIGHT TECHNOLOGY AU 0.000 0.010 0.020 0.030 0.040 0.050 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.75 AU 0.000 0.010 0.020 0.030 0.040 0.050 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 XBridge C18 5 µm, 4.6 x 150 mm Flow rate = 1.0 mL/min Solvent consumed = 15 mL/injection XBridge C18 2.5 µm, 3.0 x 75 mm Flow rate = 0.85 mL/min Solvent consumed = 3.2 mL/injection ACQUITY BEH C18 1.7 µm, 2.1 x 50 mm Flow rate = 0.61 mL/min Solvent consumed = 1.0 mL/injection Alliance ACQUITY Arc ACQUITY UPLC H-Class AU 0.000 0.010 0.020 0.030 0.040 0.050 min min min 0.30 0.40 0.50 0.60 0.70 0.80 0.90 1.00 1.10 1.20 1.30 1.40 1.50 1.60 1.70 Rs = 2.0 Rs = 2.0 Rs = 2.0 PcAve. W@50% 122 PcAve. W@50% 118 PcAve. W@50% 116 DISPERSION MATTERS: RETHINKING LC SEPARATION CATEGORIES HPLC UHPLC UPLC HPLC <6000 psi UHPLC 6000 – 15,000 psi UPLC 9000– 15,000 psi UPLC typically <1 mL/min DISPERSION IMPACT OPTIMAL PARTICLE SIZE COST PER SAMPLE OPTIMAL COLUMN I.D. TYPICAL OPERATING PRESSURE FLOW RATE HPLC typically <3 mL/min HPLC ≥3.5 µm UHPLC 1.5–5 µm UPLC <2 µm HPLC UHPLC UPLC HPLC ≥4.6 mm UHPLC 2.1– 4.6 mm UPLC 1–3 mm UHPLC typically <2 mL/min www.waters.com/lcportfolio For the best system performance, pair the right system dispersion and characteristics with the right column.