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Introduction
• Zacharias Janssen - made 1st compound
microscope
• a Dutch maker of reading glasses (late 1500’s)
Robert Hooke
• (18 July 1635 – 3 March 1703)
• Philosopher, architect and
physicist
• played an important role in the
scientific revolution through both
experimental and theoretical work
• known for his law of elasticity
• applying the word “cell" to describe
the basic unit of life.
Cork cells
Leeuwenhoek
• made a simple microscope (mid 1600’s)
• magnified 270X
• Early microscope lenses made images larger but
the image was not clear
• He is best known for his contribution to
improvement of the microscope
• and his contributions towards the
establishment of cell biology.
• he was the first to observe and
describe muscle fibres, bacteria,
spermatozoa and blood flow in
capillaries
• His microscope was used and
improved by Christiaan Huygens for
his own investigations into
microscopy.
Leeuwenhoek's microscope
A) a screw for adjusting the
height of the object being
examined
B) a metal plate serving as
the body
C) a skewer to impale the
object and rotate it
D) the lens itself, which
was spherical
van Leeuwenhoek’s microscopes were
three to five times stronger than
Hookes
allowing up to a magnification of 200
times.
enough to see one-celled animals
and even, as moving dots, bacteria.
• A simple microscope has
one lens
• Similar to a magnifying glass
• Magnification is the change
in apparent size produced by
a microscope
Light Microscope
A. cells introduction
TOTAL MAGNIFICATION
• Powers of the eyepiece (10X) multiplied by
objective lenses determine total
magnification.
Resolution
the ability to tell two points
apart as separate points.
• If the resolving power of your lens is 2um
that means two points that are 2um apart
can be seen as separate points
• If they are closer together than that, they
will blend together into one point.
• The magnification is the ability to make an
object larger.
• If the resolving power of a microscope is
poor, it will just magnify a blurry object..
A. cells introduction
A. cells introduction
ELECTRON MICROSCOPES
• More powerful; some
can magnify up to
1,000,000X
• Use a magnetic field in
a vacuum to bend
beams of electrons
• Images must be
photographed or
produced electronically
A. cells introduction
A. cells introduction
Scanning Electron Microscope (SEM)
Electron microscope image of a spider
• produces realistic 3D image
• only the surface of
specimen can be observed
Electron microscope image of a fly foot
Transmission Electron Microscope (TEM)
• produces 2D image of
thinly sliced specimen
• detailed cell parts (only
inside a cell) can be
observed
CELL THEORY
1. The basic unit of life is the cell. (Hooke)
In 1665, an English scientist
named Robert Hooke
made an improved
microscope and viewed
thin slices of cork
viewing plant cell
walls
Hooke named
what he saw
"cells"
CELL THEORY
2. All living things are made of 1 or more cells.
• Matthias Schleiden (botanist studying
plants)
• Theodore Schwann (zoologist studying
animals) stated that all living things
were made of cells
Schleiden
Schwann
CELL THEORY
3. All cells divide & come from old cells. (Virchow)
Virchow
Animal and Plant Cell Structure
Liver Cells -EM
A. cells introduction
• Click here
1mm = 1000μm
1μ m = 1000nm

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A. cells introduction

  • 2. • Zacharias Janssen - made 1st compound microscope • a Dutch maker of reading glasses (late 1500’s)
  • 3. Robert Hooke • (18 July 1635 – 3 March 1703) • Philosopher, architect and physicist • played an important role in the scientific revolution through both experimental and theoretical work • known for his law of elasticity • applying the word “cell" to describe the basic unit of life.
  • 5. Leeuwenhoek • made a simple microscope (mid 1600’s) • magnified 270X • Early microscope lenses made images larger but the image was not clear
  • 6. • He is best known for his contribution to improvement of the microscope • and his contributions towards the establishment of cell biology. • he was the first to observe and describe muscle fibres, bacteria, spermatozoa and blood flow in capillaries • His microscope was used and improved by Christiaan Huygens for his own investigations into microscopy.
  • 7. Leeuwenhoek's microscope A) a screw for adjusting the height of the object being examined B) a metal plate serving as the body C) a skewer to impale the object and rotate it D) the lens itself, which was spherical
  • 8. van Leeuwenhoek’s microscopes were three to five times stronger than Hookes allowing up to a magnification of 200 times. enough to see one-celled animals and even, as moving dots, bacteria.
  • 9. • A simple microscope has one lens • Similar to a magnifying glass • Magnification is the change in apparent size produced by a microscope
  • 12. TOTAL MAGNIFICATION • Powers of the eyepiece (10X) multiplied by objective lenses determine total magnification.
  • 13. Resolution the ability to tell two points apart as separate points. • If the resolving power of your lens is 2um that means two points that are 2um apart can be seen as separate points • If they are closer together than that, they will blend together into one point. • The magnification is the ability to make an object larger. • If the resolving power of a microscope is poor, it will just magnify a blurry object..
  • 16. ELECTRON MICROSCOPES • More powerful; some can magnify up to 1,000,000X • Use a magnetic field in a vacuum to bend beams of electrons • Images must be photographed or produced electronically
  • 19. Scanning Electron Microscope (SEM) Electron microscope image of a spider • produces realistic 3D image • only the surface of specimen can be observed Electron microscope image of a fly foot
  • 20. Transmission Electron Microscope (TEM) • produces 2D image of thinly sliced specimen • detailed cell parts (only inside a cell) can be observed
  • 21. CELL THEORY 1. The basic unit of life is the cell. (Hooke) In 1665, an English scientist named Robert Hooke made an improved microscope and viewed thin slices of cork viewing plant cell walls Hooke named what he saw "cells"
  • 22. CELL THEORY 2. All living things are made of 1 or more cells. • Matthias Schleiden (botanist studying plants) • Theodore Schwann (zoologist studying animals) stated that all living things were made of cells Schleiden Schwann
  • 23. CELL THEORY 3. All cells divide & come from old cells. (Virchow) Virchow
  • 24. Animal and Plant Cell Structure
  • 27. • Click here 1mm = 1000μm 1μ m = 1000nm