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Honors Biology Chapter 2 PowerPoint (Sections 2-1 & 2-2)
Honors Biology Chapter 2 PowerPoint (Sections 2-1 & 2-2)
• Biosphere 
• Ecosystem 
• Community 
• Population 
• Organism 
• Organ system 
• Organ 
• Tissue 
• Cell 
• Molecule 
• Atom
• Everything in the universe is made of matter. 
• Matter is anything that occupies space and has 
mass and is made of atoms! 
• Mass is the measurement of the amount of 
matter in an object. 
Matter in our galaxy
• Elements are pure substances that 
cannot be broken down chemically into 
simpler kinds of matter. 
(some things don’t count, for example: 
protons, electrons, etc.) 
• Many elements will be very familiar to you. For 
example… 
1.Helium 
2.Oxygen 
3.Gold 
4.Platinum 
5.Aluminum 
• Others will not be familiar. 
• For example… Einsteinium, Americium, Nobelium
• Over 100 elements have been identified 
• 30 are important to living things 
• 90% of the mass of living things are 
composed of 4 elements 
1. Oxygen (O) 
2. Carbon (C) 
3. Hydrogen (H) 
4. Nitrogen (N)
• Elements have chemical 
symbols 
• Composed of one or two 
letters 
• Usually taken from letters 
in the common name. 
• Sometimes taken from 
Latin or Greek name 
• Aurum (Au) for gold 
• Natrium (Na) for 
sodium 
• Elements are also identified 
by their atomic number. 
• Elements are arranged in 
the Periodic Table.
Honors Biology Chapter 2 PowerPoint (Sections 2-1 & 2-2)
• The atom is the simplest particle of an 
element that retains all of the properties 
of that element. 
• In other words, an atom is one single 
“piece” of an element. 
• For example, the smallest amount of 
carbon = 1 atom of carbon 
• Atoms are too small to be observed by 
conventional means 
• Scientists show in model form 
– Models do not show exactly what an 
atom looks like. 
– Used to predict how they will act
• The atom is broken down into 2 
major components 
1. Nucleus - contains protons 
and neutrons 
2. Electron cloud - contains 
electrons
Honors Biology Chapter 2 PowerPoint (Sections 2-1 & 2-2)
• The nucleus has 99.999% of the mass of an atom 
but little volume 
• Contains protons and neutrons 
• Protons are positively (+) charged particles 
• Neutrons are particles with no charge (0) 
• All atoms of the SAME element have the SAME 
number of protons. 
• Atomic number = number of protons in the atom 
• Mass Number = total number of protons and 
neutrons in the atom 
Atomic Number 
Chemical Symbol 
Chemical Name 
Atomic Mass or Mass Number 
C 
6 
Carbon 
12
• Electrons have a negative charge (-) 
• Very low mass, but high energy 
• Electrons are NOT in the nucleus, but in the electron cloud 
• The net electrical charge of an atom is zero (not positive or 
negative) because the atom has an equal number of 
electrons (-) and protons (+). The equal but opposite charges 
cancel each other out. 
• Number of Electrons = Number of Protons = Atomic 
Number 
• Atoms can gain or lose electrons in chemical reactions and 
become ions (more on this later)
• Isotopes are atoms of the same element 
that have a different number of neutrons. 
• The Average atomic mass of an element 
takes into account the relative amounts of 
each isotope in the element.
• Electron cloud 
• We do not really know exactly where 
the electrons are at any time in the 
atom. We only know where they 
might be. 
• Fortunately, for most chemistry it 
doesn't really matter where the 
electron actually is, we only care 
about how much energy it has. 
• It’s convenient to think that electrons 
move around the nucleus in orbits, 
like the planets in the solar system. 
• Orbitals may be misleading about 
where an electron is, but they tell us 
how much energy it has. We call this 
the Energy Level of the electron.
• Bigger Orbit = Bigger Energy Level = Higher 
Energy 
• Energy levels can hold different numbers of 
electrons 
Level 1: 2 electrons 
Level 2: 8 electrons 
• The number of electrons in the outer energy level 
determines the characteristics of the element. 
• Energy Levels are usually not filled (except noble 
gases) 
• Goal: have outer energy level full
• Very few elements exist by themselves naturally, instead, 
they are usually combined with other elements. 
• Compounds are made up of atoms of two or more 
elements in fixed proportions 
– Chemical formula shows the kinds and proportions of 
atoms of each element that forms a particular 
compound (Ex: H20)
• Compounds are usually very different from the 
elements they form from 
– Sodium – a reactive, soft , silvery metal 
– Chlorine – a reactive, poisonous green gas 
– Sodium chloride (or table salt) – stable, 
colorless crystals 
+
Honors Biology Chapter 2 PowerPoint (Sections 2-1 & 2-2)
• Most atoms are not stable in their natural state, so they 
tend to react with other atoms in different ways to form 
compounds and become more stable. 
– Remember, the goal to stability is having a full outer energy level. 
This is accomplished when atoms bond together to form 
compounds. 
• Chemical bonds are the attractive forces that hold atoms 
together 
• Electrons from the outermost energy level of the atoms 
are SHARED or TRANSFERRED whenever a bond is 
made.
• Covalent bonds are formed from atoms sharing 
electrons. 
– Share 2 electrons (1 pair) = Single Covalent Bond 
– Share 4 electrons (2 pairs) = Double Covalent Bond 
– Share 6 electrons (3 pairs) = Triple Covalent Bond 
• Usually occurs between two non-metal elements. 
Nonmetals 
Metals 
The animation above shows what happens in the formation of a covalent bond. The 
individual atoms are atoms of chlorine with only their outer level of electrons shown. 
Note that each chlorine atom has only seven outer electrons, but really wants eight.
• Ionic bonds involve the transfer of electrons 
between atoms. 
• When an atom gains or loses an electron it is 
called and ion. 
• Usually occurs between a metal and a non-metal. 
Nonmetals 
Metals
• Transferring electrons causes charges ( + or -) to 
develop 
• Opposites attract and compounds are formed!
Honors Biology Chapter 2 PowerPoint (Sections 2-1 & 2-2)
• Living things are mostly water 
• Most reactions in living things occur in water solutions 
• Water has several unique properties that make it one 
of the most important compounds found in living things 
• A solution is a mixture in which one or more 
substances are dissolved in another 
• Solvents and solutes make up solutions 
1. Solute: part of a solution that is dissolved 
(Splenda) 
2. Solvent: part of the solution that material is 
dissolved in (coffee)
• Liquids, Solids, and Gases can 
all be used in solutions. 
 Solutions can be solids 
dissolved in liquids 
Ex. Salt water 
 Solutions can be gases 
dissolved in liquids 
Ex. carbonated beverages 
 Solutions can be made from 
two solids 
Ex. Brass is a solution 
containing copper and zinc 
.
• Solutions can have varying amount of solute 
dissolved in varying amounts of solvent 
 Concentration - A measurement of the 
amount of solute dissolved in a fixed 
amount of solvent 
 2% salt solution = 2g of salt in 100mL of 
water 
 12% salt solution = 12g of salt in 100mL of 
water 
 The more solute dissolved in solution, the 
higher the concentration 
 Saturated solution - A solution where no 
more solute can be dissolved in the solvent
• Aqueous solutions are solutions that have water as the 
solvent…(aq) 
• Aqueous solutions are universally important to living 
things. 
• Fish depend on oxygen dissolved in water to survive. 
• Most nutrients plants need are in aqueous solutions in 
moist soil. 
• Body cells exist in an aqueous solution and are filled with 
aqueous solution.
• Water is made of 2 hydrogen atoms and 1 oxygen atom (H20) 
• They are covalently bonded so they share electrons. 
• The oxygen is “greedy” and pulls the electrons closer to it. 
 The oxygen is therefore a little bit negative, and the 
hydrogens are a little bit positive. 
 Because of this uneven distribution of charge, water is 
called a polar molecule.
• The polar nature of water allows 
it to dissolve polar substances, 
such as sugars, ionic 
compounds, and some proteins. 
• Water does not dissolve 
nonpolar substances, such as 
fats like oil.
Honors Biology Chapter 2 PowerPoint (Sections 2-1 & 2-2)
• The polar nature of water 
causes water molecules to 
be attracted to one another. 
• Opposites attract: the 
oxygen of one molecule is 
attracted to a hydrogen of 
another. This attraction 
results in a hydrogen bond.
• Hydrogen bonds in water exert an attractive force strong enough that water 
“sticks” to itself and other substances! 
 Cohesion - An attractive force that holds molecules of a single 
substance together. 
- Ex: water molecules stick to each other 
 Cohesion causes “surface tension” or a thin “skin” on the surface of 
water. 
 Adhesion - An attractive force between two particles of different 
substances. 
- Ex: water molecules stick to glass molecules 
 Adhesion causes capillarity, which results in the rise of the surface of a 
liquid when in contact with a solid.
• Because of its hydrogen bonds, water has a high 
heat capacity, which means that water can absorb 
or release large amounts of energy in the form of 
heat with only a slight change in its temperature. 
• During a hot day, water can absorb heat (hydrogen 
bonds break) and cool the air. At night, the water 
cools (hydrogen bonds reform) and releases heat 
into the air. 
• Earth’s oceans stabilize global temperatures enough 
for life to exist. 
• Water’s high heat capacity allows your cells to keep 
an even temperature despite changes in the 
environment.
• Solid water is less 
dense than liquid 
water. 
– This is opposite of all 
other substances 
• Hydrogen bonding 
causes ice crystals to 
have large amounts 
of open space.
• When bodies of water freeze, they freeze 
from the top down and not the bottom up. 
• Ice insulates the water below from the cold 
air, which allows fish and other aquatic 
animals to survive under the icy surface.
• The alkalinity or acidity of a solution can determine 
the survival or death of organisms! 
• What do we mean when we say acidic and alkaline 
(basic)?
• Water molecules bump into each other and can actually break 
each other apart! This results in a hydroxide and hydrogen ion. 
• An ion is any atom(s) that have a positive or negative charge 
– Hydroxide ion is the OH- 
– Hydrogen ion is the H+ 
• The hydrogen ion (H+) can become attracted to the oxygen in 
another water molecule resulting in a hydronium ion. 
– Hydronium ion is the H3O+ 
• This process is called the ionization of water
Honors Biology Chapter 2 PowerPoint (Sections 2-1 & 2-2)
• The relative concentrations of hydronium (H3O+) 
and hydroxide (OH-) ions in a solution 
determines if it is an acid or a base. 
• Pure water contains an equal number of both, so 
it is a NEUTRAL solution.
• Some compounds, when they dissolve in water will 
separate and form H+ ions. These compounds are called 
acids. 
• H+ will react with H20 to form H30+ 
– When HCl gas dissolves in water, it breaks up into H+ 
and Cl- 
– H+ reacts with H20 to form H3O+. There is now more 
H3O+ ions than OH- ions. 
• There are always more H3O+ than OH- in acidic solutions. 
Some common examples: 
Hydrochloric Acid = HCl 
Sulfuric Acid = H2SO4 
Nitric Acid = HNO3 
Acetic Acid = HCH3OO 
Phosphoric Acid = H3PO4
• Called acidic 
• Acids tend to taste sour 
• Concentrated acids are very 
corrosive 
– HCl in your stomach helps to 
breakdown and digest proteins 
• Examples: Orange juice, vinegar, 
soda 
• Drinking acidic drinks over a long 
period of time can erode the tooth 
enamel!
• Some compounds, when they dissolve in water and 
separate will form OH- ions. These compounds are called 
Bases. 
• When the solid NaOH dissolves in water, it 
breaks up into Na+ and OH- 
• There are now more OH- than H3O+ ions. 
• There are always more OH- than H3O+ in basic 
solutions. 
Some common examples: 
Barium Hydroxide = Ba(OH)2 
Sodium Hydroxide = NaOH 
Potassium Hydroxide = KOH 
Calcium Hydroxide = 
Ca(OH)2
• Called alkaline 
• Slippery sensation when touched 
– Bases react with oils in skin to form soap 
• Bases have a bitter taste 
• Examples: Soap, antacids (Magnesium hydroxide 
and Aluminum hydroxide), ammonia
• Scientists have developed a 
scale for comparing hydronium 
and hydroxide ions in solution. 
• pH scale 
– Measures from 0 to 14 
– Below 7 is acidic 
– Above 7 is basic 
– 7 is neutral 
– Logarithmic scale (tenfold 
change per number) 
– pH of 4 is 100 times more 
acidic (H3O+) than a pH of 6
• How much more acidic is 
vinegar compared to urine? 
• Difference of 3 pH levels = 
10 x 10 x 10 = 1000 times 
more acidic!
Acid + Base = A Neutral Solution (water and a salt) 
Acid Base Water Salt 
HCl + NaOH H2O + NaCl 
HBr + KOH H2O + KBr
• Living systems are very sensitive to 
pH because enzymes can only 
function in very specific pH ranges. 
Uh oh, I think 
we’re gonna be in 
pH 4 soon! But how will we 
function? That is not 
acidic enough, Help!
• Living things use buffers to prevent pH from 
changing too much. 
• Buffers are chemical substances that 
neutralize small amounts of acids or bases in a 
solution. 
• Complex buffering systems keep you body’s 
pH values at the right level. 
• pH varies by body system 
 The pH of the human stomach is usually 
between 2 and 3. 
 Ideally, the pH of the blood should be 
maintained at 7.4. If the pH drops below 6.8 
or rises above 7.8, death may occur.
Honors Biology Chapter 2 PowerPoint (Sections 2-1 & 2-2)

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Honors Biology Chapter 2 PowerPoint (Sections 2-1 & 2-2)

  • 3. • Biosphere • Ecosystem • Community • Population • Organism • Organ system • Organ • Tissue • Cell • Molecule • Atom
  • 4. • Everything in the universe is made of matter. • Matter is anything that occupies space and has mass and is made of atoms! • Mass is the measurement of the amount of matter in an object. Matter in our galaxy
  • 5. • Elements are pure substances that cannot be broken down chemically into simpler kinds of matter. (some things don’t count, for example: protons, electrons, etc.) • Many elements will be very familiar to you. For example… 1.Helium 2.Oxygen 3.Gold 4.Platinum 5.Aluminum • Others will not be familiar. • For example… Einsteinium, Americium, Nobelium
  • 6. • Over 100 elements have been identified • 30 are important to living things • 90% of the mass of living things are composed of 4 elements 1. Oxygen (O) 2. Carbon (C) 3. Hydrogen (H) 4. Nitrogen (N)
  • 7. • Elements have chemical symbols • Composed of one or two letters • Usually taken from letters in the common name. • Sometimes taken from Latin or Greek name • Aurum (Au) for gold • Natrium (Na) for sodium • Elements are also identified by their atomic number. • Elements are arranged in the Periodic Table.
  • 9. • The atom is the simplest particle of an element that retains all of the properties of that element. • In other words, an atom is one single “piece” of an element. • For example, the smallest amount of carbon = 1 atom of carbon • Atoms are too small to be observed by conventional means • Scientists show in model form – Models do not show exactly what an atom looks like. – Used to predict how they will act
  • 10. • The atom is broken down into 2 major components 1. Nucleus - contains protons and neutrons 2. Electron cloud - contains electrons
  • 12. • The nucleus has 99.999% of the mass of an atom but little volume • Contains protons and neutrons • Protons are positively (+) charged particles • Neutrons are particles with no charge (0) • All atoms of the SAME element have the SAME number of protons. • Atomic number = number of protons in the atom • Mass Number = total number of protons and neutrons in the atom Atomic Number Chemical Symbol Chemical Name Atomic Mass or Mass Number C 6 Carbon 12
  • 13. • Electrons have a negative charge (-) • Very low mass, but high energy • Electrons are NOT in the nucleus, but in the electron cloud • The net electrical charge of an atom is zero (not positive or negative) because the atom has an equal number of electrons (-) and protons (+). The equal but opposite charges cancel each other out. • Number of Electrons = Number of Protons = Atomic Number • Atoms can gain or lose electrons in chemical reactions and become ions (more on this later)
  • 14. • Isotopes are atoms of the same element that have a different number of neutrons. • The Average atomic mass of an element takes into account the relative amounts of each isotope in the element.
  • 15. • Electron cloud • We do not really know exactly where the electrons are at any time in the atom. We only know where they might be. • Fortunately, for most chemistry it doesn't really matter where the electron actually is, we only care about how much energy it has. • It’s convenient to think that electrons move around the nucleus in orbits, like the planets in the solar system. • Orbitals may be misleading about where an electron is, but they tell us how much energy it has. We call this the Energy Level of the electron.
  • 16. • Bigger Orbit = Bigger Energy Level = Higher Energy • Energy levels can hold different numbers of electrons Level 1: 2 electrons Level 2: 8 electrons • The number of electrons in the outer energy level determines the characteristics of the element. • Energy Levels are usually not filled (except noble gases) • Goal: have outer energy level full
  • 17. • Very few elements exist by themselves naturally, instead, they are usually combined with other elements. • Compounds are made up of atoms of two or more elements in fixed proportions – Chemical formula shows the kinds and proportions of atoms of each element that forms a particular compound (Ex: H20)
  • 18. • Compounds are usually very different from the elements they form from – Sodium – a reactive, soft , silvery metal – Chlorine – a reactive, poisonous green gas – Sodium chloride (or table salt) – stable, colorless crystals +
  • 20. • Most atoms are not stable in their natural state, so they tend to react with other atoms in different ways to form compounds and become more stable. – Remember, the goal to stability is having a full outer energy level. This is accomplished when atoms bond together to form compounds. • Chemical bonds are the attractive forces that hold atoms together • Electrons from the outermost energy level of the atoms are SHARED or TRANSFERRED whenever a bond is made.
  • 21. • Covalent bonds are formed from atoms sharing electrons. – Share 2 electrons (1 pair) = Single Covalent Bond – Share 4 electrons (2 pairs) = Double Covalent Bond – Share 6 electrons (3 pairs) = Triple Covalent Bond • Usually occurs between two non-metal elements. Nonmetals Metals The animation above shows what happens in the formation of a covalent bond. The individual atoms are atoms of chlorine with only their outer level of electrons shown. Note that each chlorine atom has only seven outer electrons, but really wants eight.
  • 22. • Ionic bonds involve the transfer of electrons between atoms. • When an atom gains or loses an electron it is called and ion. • Usually occurs between a metal and a non-metal. Nonmetals Metals
  • 23. • Transferring electrons causes charges ( + or -) to develop • Opposites attract and compounds are formed!
  • 25. • Living things are mostly water • Most reactions in living things occur in water solutions • Water has several unique properties that make it one of the most important compounds found in living things • A solution is a mixture in which one or more substances are dissolved in another • Solvents and solutes make up solutions 1. Solute: part of a solution that is dissolved (Splenda) 2. Solvent: part of the solution that material is dissolved in (coffee)
  • 26. • Liquids, Solids, and Gases can all be used in solutions.  Solutions can be solids dissolved in liquids Ex. Salt water  Solutions can be gases dissolved in liquids Ex. carbonated beverages  Solutions can be made from two solids Ex. Brass is a solution containing copper and zinc .
  • 27. • Solutions can have varying amount of solute dissolved in varying amounts of solvent  Concentration - A measurement of the amount of solute dissolved in a fixed amount of solvent  2% salt solution = 2g of salt in 100mL of water  12% salt solution = 12g of salt in 100mL of water  The more solute dissolved in solution, the higher the concentration  Saturated solution - A solution where no more solute can be dissolved in the solvent
  • 28. • Aqueous solutions are solutions that have water as the solvent…(aq) • Aqueous solutions are universally important to living things. • Fish depend on oxygen dissolved in water to survive. • Most nutrients plants need are in aqueous solutions in moist soil. • Body cells exist in an aqueous solution and are filled with aqueous solution.
  • 29. • Water is made of 2 hydrogen atoms and 1 oxygen atom (H20) • They are covalently bonded so they share electrons. • The oxygen is “greedy” and pulls the electrons closer to it.  The oxygen is therefore a little bit negative, and the hydrogens are a little bit positive.  Because of this uneven distribution of charge, water is called a polar molecule.
  • 30. • The polar nature of water allows it to dissolve polar substances, such as sugars, ionic compounds, and some proteins. • Water does not dissolve nonpolar substances, such as fats like oil.
  • 32. • The polar nature of water causes water molecules to be attracted to one another. • Opposites attract: the oxygen of one molecule is attracted to a hydrogen of another. This attraction results in a hydrogen bond.
  • 33. • Hydrogen bonds in water exert an attractive force strong enough that water “sticks” to itself and other substances!  Cohesion - An attractive force that holds molecules of a single substance together. - Ex: water molecules stick to each other  Cohesion causes “surface tension” or a thin “skin” on the surface of water.  Adhesion - An attractive force between two particles of different substances. - Ex: water molecules stick to glass molecules  Adhesion causes capillarity, which results in the rise of the surface of a liquid when in contact with a solid.
  • 34. • Because of its hydrogen bonds, water has a high heat capacity, which means that water can absorb or release large amounts of energy in the form of heat with only a slight change in its temperature. • During a hot day, water can absorb heat (hydrogen bonds break) and cool the air. At night, the water cools (hydrogen bonds reform) and releases heat into the air. • Earth’s oceans stabilize global temperatures enough for life to exist. • Water’s high heat capacity allows your cells to keep an even temperature despite changes in the environment.
  • 35. • Solid water is less dense than liquid water. – This is opposite of all other substances • Hydrogen bonding causes ice crystals to have large amounts of open space.
  • 36. • When bodies of water freeze, they freeze from the top down and not the bottom up. • Ice insulates the water below from the cold air, which allows fish and other aquatic animals to survive under the icy surface.
  • 37. • The alkalinity or acidity of a solution can determine the survival or death of organisms! • What do we mean when we say acidic and alkaline (basic)?
  • 38. • Water molecules bump into each other and can actually break each other apart! This results in a hydroxide and hydrogen ion. • An ion is any atom(s) that have a positive or negative charge – Hydroxide ion is the OH- – Hydrogen ion is the H+ • The hydrogen ion (H+) can become attracted to the oxygen in another water molecule resulting in a hydronium ion. – Hydronium ion is the H3O+ • This process is called the ionization of water
  • 40. • The relative concentrations of hydronium (H3O+) and hydroxide (OH-) ions in a solution determines if it is an acid or a base. • Pure water contains an equal number of both, so it is a NEUTRAL solution.
  • 41. • Some compounds, when they dissolve in water will separate and form H+ ions. These compounds are called acids. • H+ will react with H20 to form H30+ – When HCl gas dissolves in water, it breaks up into H+ and Cl- – H+ reacts with H20 to form H3O+. There is now more H3O+ ions than OH- ions. • There are always more H3O+ than OH- in acidic solutions. Some common examples: Hydrochloric Acid = HCl Sulfuric Acid = H2SO4 Nitric Acid = HNO3 Acetic Acid = HCH3OO Phosphoric Acid = H3PO4
  • 42. • Called acidic • Acids tend to taste sour • Concentrated acids are very corrosive – HCl in your stomach helps to breakdown and digest proteins • Examples: Orange juice, vinegar, soda • Drinking acidic drinks over a long period of time can erode the tooth enamel!
  • 43. • Some compounds, when they dissolve in water and separate will form OH- ions. These compounds are called Bases. • When the solid NaOH dissolves in water, it breaks up into Na+ and OH- • There are now more OH- than H3O+ ions. • There are always more OH- than H3O+ in basic solutions. Some common examples: Barium Hydroxide = Ba(OH)2 Sodium Hydroxide = NaOH Potassium Hydroxide = KOH Calcium Hydroxide = Ca(OH)2
  • 44. • Called alkaline • Slippery sensation when touched – Bases react with oils in skin to form soap • Bases have a bitter taste • Examples: Soap, antacids (Magnesium hydroxide and Aluminum hydroxide), ammonia
  • 45. • Scientists have developed a scale for comparing hydronium and hydroxide ions in solution. • pH scale – Measures from 0 to 14 – Below 7 is acidic – Above 7 is basic – 7 is neutral – Logarithmic scale (tenfold change per number) – pH of 4 is 100 times more acidic (H3O+) than a pH of 6
  • 46. • How much more acidic is vinegar compared to urine? • Difference of 3 pH levels = 10 x 10 x 10 = 1000 times more acidic!
  • 47. Acid + Base = A Neutral Solution (water and a salt) Acid Base Water Salt HCl + NaOH H2O + NaCl HBr + KOH H2O + KBr
  • 48. • Living systems are very sensitive to pH because enzymes can only function in very specific pH ranges. Uh oh, I think we’re gonna be in pH 4 soon! But how will we function? That is not acidic enough, Help!
  • 49. • Living things use buffers to prevent pH from changing too much. • Buffers are chemical substances that neutralize small amounts of acids or bases in a solution. • Complex buffering systems keep you body’s pH values at the right level. • pH varies by body system  The pH of the human stomach is usually between 2 and 3.  Ideally, the pH of the blood should be maintained at 7.4. If the pH drops below 6.8 or rises above 7.8, death may occur.