Columns in HPLC
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This document provides information about different types of columns used in high performance liquid chromatography (HPLC). It discusses normal phase and reverse phase chromatography columns. It describes various column packing materials, particle sizes, dimensions, costs and specifications. It provides details on columns from several major manufacturers like Waters, Phenomenex, Agilent, GE Healthcare and others. Preparative chromatography is also briefly mentioned. Resources for further information are listed at the end.
Columns in HPLC
- 3. HELLO! Varad Bende TE Biotech Department of Biotechnology Jawaharlal Nehru Engineering College , Aurangabad MS 431003 Let us put a glance over Columns used in HPLC.
- 5. • The Brain of HPLC • Resolves a mixture into its different components • Available in different material packings and sizes according to the requirements
- 7. Analyte of Interest • Chemical Properties • Physical Properties Type of chromatography Purpose of chromatography Amount of Sample Precision needed
- 8. Advanced Chromatography Technologies Agela Technologies Agilent Applied Biosystems Beckman Coulter Bio-Rad Biotage ChiralTech Eksigent ES Industries Grace Discovery Sciences Hamilton Imtakt J & K Scientific Kromasil Life Technologies MAC-MOD Peeke Scientific PerkinElmer Phenomenex Restek Shiseido Shodex Sigma-Aldrich SiliCycle Thermo Fisher Scientific Tosoh Bioscience Valco Waters Zirchrom (Reference - www.labmanager.com)
- 9. Particle Size 2.5 to 10 μm 2.5 , 3.5 , 5 μm 2.7 μm Packing Material C4 C8 C18 Phenyl Amide C18 Cyano PFP T3 Phenyl C18+ C18 T3 C8 Technology Ethylene Bridged Hybrid • Charged Surface Hybrid(CSH) • High Strength Silica (HSS) Solid Core Technology Dimensions 75μm*5mm to 30*300 mm 1*5 mm to 50*250 mm 2..1*30 mm to 4.6*150 mm Cost ₹ 43,500 to 1,90,000 ₹ 34,000 to 11,00,000 ₹ 32,000 to 76,000 Specificatios • Analytical and Preparative • Robust • High pH stability (1-12) • BioSeparation • Analytical and Preparative • Reverse phase • Designed for selectivity • Highest analyte mass loading • High efficiency • Reverse Phase • Compatible with UPLC
- 12. Infinity Lab •2.1 * 50 mm to 4.6 * 250 mm •Particle Size 4 μm •Cost ₹ 20,000 to 1,00,000 •Packing Materials - C8 C18 PhenylHexyl •Long life • 2.1 * 50 mm to 4.6 * 250 mm • Particle Size 3.5 and 5 μm • Cost ₹ 20,000 to 1,00,000 • Packing Materials - C8 C18 PhenylHexyl PAH • Excellent chromatography with mixtures of polar and nonpolar analytes • Particle Size 3.5 and 5 μm • Cost ₹ 24,000 to 56,000 • Packing Materials - C8 C18 PhenylHexyl
- 14. Material Packings - C8 C18 Amide Cyano Silica Particle size – 2.7 – 5μm Cost 26,000 to 3,00,000 High purity silica Modern bonding processes Analytical and Preparative
- 15. TM • Packing Material – C5 C8 C18 • Particle Size – 3-5 um • Cost - 30,000 to 50,000 • Aqueous compatible
- 16. SupelcoSIL TM • Packing Material – C8 C18 Phenyl • Particle size – 40,000 to 70,000 • Silica Based • Micro to Preparative scale • High Quality
- 18. • Ultra-pure silica-based HPLC columns • Particle Size - 3 to 5μm • Available in C4 C5 C8 C18 PhenylHexyl Silica CN Amino • Cost – 5,000 to 50,000 • Wide pH stability for long column lifetime and method flexibility • Easy method scalability from Fast LC to Preparative and Bulk Purification
- 19. • Uses Core Shell Technology along with Ethane Cross Linking • 2.6 , 4 , 5μm • C8 C18 Polar • Analytical and preparative • Aqueous Stability • Improved peak shapes
- 20. • Accucore • Acclaim • BetaBasic • BioBasic • Hypersil BDS • Hypersil GOLD • Syncronis • Hypercarb • HyperRez • HyPURITY
- 21. Accucore™ LC Columns Particle Size - 2.6µm solid-core particles Material - C8 C18 PhenylHexyl C30 etc Dimensions - 2 * 50 mm to 4.6 * 150 mm Price - 20,000 to 50,000 Synchronis™ LC Columns • Highly pure, high surface area silica • Dense bonding and double endcapping • Stringent manufacturing and testing protocols- manufactured, packaged and tested in ISO9000 accredited facilities Columns: C18, C8, Phenyl, Amino, Silica Acclaim™ LCColumns Particle Size - 2.2µm Porous silica particles with advanced and innovative column bonding technologies Material - C8 C18 PhenylHexyl C30 etc Dimensions - 2 * 50 mm to 4.6 * 150 mm Price - 20,000 to 50,000
- 23. • Hybrid Silica based HPLC Columns • Multi-layered, organic/inorganic hybrid particle • Double Endcapped • Wider pH and Temperature Range • Material - C18 C8 Phenyl Silica • Particle Size - 3 , 5 , 10 , 50μm • Dimensions – 2*20 mm to 4.6*250 mm (30*250 mm in Preparative) • Cost – 20,000 to 50,000 (Upto 10,00,000 in Preparative)
- 24. CoreShell Columns by YMC • Particle Size – 2.7μm • Dimensions - 2.6*40 to 4.6*150 • Material - C8 and C18 • Cost - 21,000 to 40,000 • Coreshell Technology • Ultra fast separation with outstanding resolution • Excellent peak shape for basic and coordination compounds • Wide pH application range • Low column bleed, ideal for LC/MS
- 25. YMC Pro Columns • Particle size - 2 , 3 , 4μm • Materials - C8 C18 • High performance , reliable columns designed for the most demanded HPLC • Novel lewis acid/lewis base endcapping • Sharp and symmetrical peaks
- 26. Analyte of Interest • Chemical Properties • Physical Properties Type of chromatography Purpose of chromatography Amount of Sample Precision needed
- 28. • Waters • Agilent • Phenomenex • Sigma Aldrich • GE Life Sciences • Altech • Shimadzu • Fisher Scientific • YMC • Perkin Elmer (Reference – www.labx.com)
- 29. YMC, Japan • Waters • ThermoFisher Massachusetts USA• Phenomenex • Agilent California USA GE Healthcare UK Sigma Aldrich Missouri USA ACE Scottland
- 32. www.labmanager.com Web Resources www.labmanager.com www.labx.com www.waters.com www.crawfordscientific-secure.com http://www.agilent.com www.thermofisher.com www.ymc.co.jp www.phenomenex.com www.sigmaaldrich.com
- 33. THANKS! Any questions? You can find me at » varad93@rediffmail.com