CPCSEA guidelines ppt

By Dhruvi Parmar
M.Pharm 1st sem
CPCSEA Guidelines
GUIDED BY:
KHUSHBUTHAKOR

INTRODUCTION
The Committee for the Purpose of Control and
Supervision of Experiments on animals (CPCSEA)
is a statutory body formed by the act of the Indian
Parliament under the Prevention of cruelty to
animals act 1960.

CORE MEMBERS:
 Smt. Maneka Gandhi – Chair person
 Mr. A.K.Joshi- member Secretary
SUB-COMMITTEE MEMBERS:
 Dr. Manju Sharma (Secretary, Department of
biotechnology members)
 Dr vasanth Muthuswamy ( Sr. DDG, Indian Council
of Medical Research)

OBJECTIVES
 The goal of these guidelines is to promote the
human care of animal use in biomedical and
behavioural research and testing.
 To avoid unnecessary pain before, during and after
experiment.
 Guidelines for housing, care, breeding and
maintenance of animals should be provided.
 To regulate experimentation on the animal, the
committee has formulated “Breeding of &
experiments on animals(control & supervision)
rules, 1998” which were amended in 2001 & then in
2006.

FUNCTIONS
 Registration of establishments conducting animal
experimentation or breeding of animals for this purpose.
 Selection and appointment of nominees in the Institutional
Animal Ethics Committees of registered establishments.
 Approval of Animal House Facilities on the basis of reports of
inspections conducted by CPCSEA.
 Permission for conducting experiments involving use of
animals.
 Recommendation for import of animals for use in experiments.
 Action against establishments in case of violation of any legal
norm/stipulation.

QUARANTINE, STABILIZATION AND
SEPARATION
1. Quarantine is the separation of newly received
animals from those already in the facility until the
health and possibly the microbial status of the newly
received animals have been determined.
2. An Effective quarantine minimizes the chance for
introduction of pathogens into an established colony.
3. The duration at quarantine in small lab animals from
one week to one month to one week to one month and
large animals allowed up to 6 weeks.
4. For non-human primates the period varies from 2 to 3
months depending on the reaction of TB testing

5. Physical separation of animals by species is
recommended to prevent interspecies disease
transmission and to eliminate anxiety and possible
physiological and behavioural changes due to
interspecies conflict.
6. Different species should ideally be housed in different
rooms; however, cubicles, laminar-flow units, cages
that have filtered air or separate ventilation, and
isolators shall be suitable alternatives.

SURVEILLANCE, DIAGNOSIS, TREATMENT AND
CONTROL OF DISEASE
 All animals should be observed for signs of illness, injury,
or abnormal behaviour.
 Unexpected deaths and signs of illness should be
reported; Post-mortem examination should be reported
for timely delivery of veterinary medical care.
 If animals are known to be exposed to an infectious
agent the group should be kept intact and isolated during
the process of diagnosis, treatment, and control.
 Diagnosis clinical laboratory may be made available.

ANIMAL HUSBANDRY
1. Caging and outdoor housing:
 Caging should be designed carefully to facilitate
animal well being, and also to minimize experimental
variables
 Polypropylene, polycarbonates and stainless steel
cages should be used to house small lab animals.
 Outer housing should be accessible to all animals with
sufficient ventilation, furnishing and to prevent the
accumulation of waste materials.
2. Social environment:
 The social environment includes all interactions
among those able to communicate.
 Population density can affect reproduction,
Metabolism, immune response & behaviour.

 Non-human primate should have a ranging activites:
i. FOOD
ii. BEDDING
iii. WATER
iv. SANITATION & CLEANLINESS
3. Waste disposal:
 Waste should be removed regularly and frequently.
 Most preferred method of waste disposal is
incineration.
 Hazardous waste should be rendered safe by
sterilization and decontamination.
4. Pest control:
 Adaptation of programs designed to prevent, control
or eliminate the presence of or infestations by pests
are essential in an animal home environment.

5. Record Keeping:
• Animal House plans, which includes typical floor plan,
all fixtures etc.
• Animal House staff record - both technical and non -
technical
• Health record of staff and animals
• All SOPs relevant to experiments, care, breeding and
management of animals
• Breeding, stock, purchase and sales records
• Minutes of institutional Animals Ethics Committee
Meetings
• Records of experiments conducted with the number of
animals used (copy of Form D)
• Mortality, Post-mortem Record, wherever required.
• Clinical record of sick animals.
• Health monitoring Records.
• Rehabilitation Records, wherever required.

ANIMAL HUSBANDRY AND MANAGEMENT
(A).ANIMAL CARE AND TECHNICAL PERSONNEL
 Animal care programs require technical and
husbandry support.
 Institutions should employ people trained in
laboratory animal science or provide for both formal
and on-the-job training to ensure effective
implementation of the program.
 Institutions should have policies governing
experimentation with hazardous agents
 Personnel should change clothing as often as is
necessary to maintain personal hygiene.

(B)SANITATION AND CLEANLINESS
o Animal room, corridors, storage spaces and other
areas should be cleaned with appropriate detergents
and disinfectants.
o For larger animals, such as dogs, cats, and non-
human primates, soiled litter material should be
removed twice daily.
o Water bottles, sipper nozzles stoppers, and other
watering equipment should be wasted and then
sanitized be rinsing with water of at least 82.2˚C
(180˚F) or appropriated chemical agents to destroy
pathogenic organisms.
o Sanitation practices should be monitored appropriately

(B).VETERINARY CARE
 Adequate veterinary care must be provided and is
the responsibility of a veterinarian or a person who
has training or experience `in laboratory animal
sciences and medicine.
 Daily observation of animals can be accomplished
by someone other than a veterinarian:
 Animal must be observed regularly for sign of illness,
injury, or abnormal behaviour.
 Any anomalies must be reported to veterinarian.
 In case of contagious disease-animal must be isolated
from healthy animal.

(D).ANIMAL PROCUREMENT
 All animals must be acquired lawfully as per the
CPCSEA guidelines.
 A health surveillance program for screening
incoming animals should be carried out before
purchase to assess animal quality.
 Each consignment of animals should be inspected
for compliance with procurement specifications, and
the animals should be quarantined and stabilized
according to procedures appropriate for the species
and circumstances.

(E).PERSONNEL AND TRAINING
o The selection of animal facility staff, particularly the
staff working in animal rooms or involved in
transportation, is a critical component in the
management of an animal facility.
o The staff must be provided with all required
protective clothing while working in animal rooms.
o The persons working in animal house should not
eat, drink, smoke in animal room and have all
required vaccination, particularly against Tetanus
and other zoonotic diseases.

(F).TRANSPORT OF LABORATORY ANIMALS
 The main considerations for transport of animals
are, mode of transport, containers, animal density
in cages, food and water during transit, protection
from transit infections, injuries and stress.
 The food and water should be provided in suitable
containers or in suitable form so as to ensure that
they get adequate food and more particularly fluid
during transit.
 The transport of animals from one place to another
is very important and must be undertaken with care.

(G).STANDARD OPERATING PROCEDURES
(SOP’S):
A SOP should contain the following items:
 Name of the Author
 Title of the SOP
 Date of approval
 Reference of previous SOP on the same subject and date
(Issue number and Date)
 Location and distribution of SOP’s with sign of each recipient.
 Objectives
 Detailed information of the instruments used in relation with
animals with methodology (Model no., Serial no., Date of
commissioning, etc)
 The name of the manufacturer of the reagents and the
methodology of the analysis pertaining to animals
 Normal value of all parameters
 Hazard identification and risk assessment

(H).ANAESTHESIA AND EUTHANASIA:
1. ANESTHESIA:
• Anesthesia is a medical treatment/process that
prevents organism from feeling pain during surgery
• It must also be ensured that the anaesthesia is given for
the full duration of experiment and at no stage the
animal is conscious to perceive pain during the
procedure.
• Neuromuscular blocking agents must not be used
without adequate general anaesthesia.
2. EUTHANASIA:
• Euthanasia should be resorted to events where an
animal is required to be sacrificed to reduce suffering
or to limit spread of infections or for termination of an
experiment or for other ethical reasons.

 The method should in all cases meet the following
requirements:
 (a) Death, without causing anxiety, pain or distress
with minimum time lag phase.
 (b) Minimum physiological and psychological
disturbances.
 (c) Compatibility with the purpose of study and
minimum emotional effect on the operator.
 (d) Location should be separate from animal rooms
and free from environmental contaminants.
 Tranquilizers have to be administered to larger
species such as monkeys, dogs and cats before a
procedure of euthanasia.

CPCSEA guidelines ppt

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CPCSEA guidelines ppt