DNA BARCODING INTRODUCTION
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DNA barcoding is a method of species identification using a short section of DNA from a specific gene or genes. The premise is that a DNA sequence can be used to uniquely identify an organism by comparing it to a reference library of DNA sequences. For animals, the cytochrome c oxidase 1 gene is commonly used, while plants may use the rbcL, matK, or trnH-psbA genes. Fungi typically use the internal transcribed spacer region. The process involves isolating DNA from a sample, amplifying the target region via PCR, sequencing the results, and comparing the sequence to reference databases to identify the matching species. Applications include taxonomy, pest/disease control, food safety, conservation,
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DNA BARCODING INTRODUCTION
- 1. DNA BARCODING Dr. ABDULJALEEL K Assistant Professor Dept. of Zoology Government College, Kasaragod
- 2. Dr. Paul Hebert. Father of DNA barcoding https://www.univcan.ca/wp-content/uploads/2019/03/GEI-2018-winners-Paul-Hebert.jpg
- 3. DNA barcoding • A method of species identification using a short section of DNA from a specific gene or genes. • The premise of DNA barcoding is that by comparison with a reference library of such DNA sequence, an individual sequence can be used to uniquely identify an organism to species,
- 4. Animal barcoding studies • Use a region in the mitochondrial cytochrome c oxidase 1 gene (“CO1”). • 16 S rRNA etc
- 5. Plant barcoding studies • use one or a few plastid regions • e.g. rbcL and matK, and the non-coding spacer trnH-psbA) • the internal transcribed spacer (ITS) region of nuclear ribosomal DNA.
- 6. Fungal barcoding studies • Use the internal transcribed spacer (ITS) region in the nuclear ribosomal cistron.
- 7. Steps • 1: Isolate DNA from the sample • 2: Amplify the target DNA barcode region using PCR • 3: Sequence the PCR products • 4: Compare the resulting sequences against reference databases to find the matching species
- 12. Applications • Taxonomy and species identification • Pest and disease control • Food production and safety • Resource management • Conservation • Research • Education • Recreation.
- 13. References • https://ibol.org/about/dna-barcoding/ • https://en.m.wikipedia.org/wiki/DNA_barcoding • https://www.univcan.ca/wp-content/uploads/2019/03/GEI-2018-winners- Paul-Hebert.jpg