Ensuring a safe surgical environment requires proper cleaning, hand washing, and sterilization of instruments. This includes cleaning the operating room before and after cases, as well as in between patients. Proper hand washing, especially surgical hand washing, is critical to remove microorganisms. Instruments must be cleaned, disinfected, and sterilized based on their classification as critical, semi-critical, or non-critical. Various methods can be used for sterilization including steam, hydrogen peroxide gas, and dry heat. Adhering to protocols at each step helps minimize infection risk and ensure safety for both patients and staff.