Introduction to the light microscope
- 2. Parts of a binocular microscope
- 3. Binocular body Rotating Nose Piece (turret) Objective lenses Stage clips for slides Stage Fine focus Coarse focus Y axis Stage adjustment Condenser X axis knobs Light source Rheostat Base
- 4. Condenser Aperture iris diaphragm in condenser Rheostat Controls brightness Light source
- 5. Width control Individual focus Ocular Lenses
- 6. • Image is produced when light is passed through specimen. • Contrast is improved with staining techniques
- 7. Image Production in a Compound Light Microscope • Light from the source is focused on the specimen by the condenser lens. • It then enters the objective lens, where it is magnified to produce a real image. • The real image is magnified again by the ocular lens to produce a virtual image that is seen by the eye.
- 8. 10X • The amount of magnification is marked 10x X 40x = 400x on each lens 40X • Total Magnification = Objective X Ocular
- 9. • Resolution: clarity of image • Limit of resolution (resolving power): how far apart two points must be for the microscope to view them as being separate • Light microscope = 0.2 um
- 10. • NA = lens’s ability to “capture” light coming from specimen to make image • Oil immersion increases NA, therefore increases its resolving power.
- 11. Making a Wet Mount • Hold cover slip at 45 degree angle • Slowly lower cover slip to prevent bubbles • (click on title above to view animation)