IP GROUP PLC WORK PLACEMENT ON 3D PRESENTATION
- 2. “In simple terms it is the use of computer aided transfer process (3D printer) for patterning and assembling living and non living materials with a predefined 3D organization in order to produce bio-engineered structures that can be used in regenerative medicine and tissue engineering”
- 5. Bio printing represent a big paradigm shift when compared to traditional approaches in tissue engineering. Correct positioning of cell within a scaffold. ‘Layer by layer’ approach allows for precise organisation of individual elements of the tissue. Step towards ‘off the shelf organs/tissues’ for transplantation and drug testing.
- 6. The IP gorup life sciences team identified ‘3D tissue/bio printing’ as an area with significant potential to disrupt the healthcare market in the near future. Therefore a 2 week placement targeted to inform IP group investment strategy and due diligence in this sector was undertaken.
- 7. Map the technology space ◦ Who are the leaders in the field ? Academic groups, corporate R&D, spin outs, companies, etc. Who are the key people ? Area of expertise ? Collaborations ? ◦ How mature is each technology area ? Building blocks to commercialise the tech if any ? Technical challenges arisen ? ◦ What application are being targeted ? Tissue/ organs ? Now & future ? ◦ Any overlaps or obvious synergies in whats out there and current investments that IP group already have ? What's missing ?
- 8. Basis of assessment of individual companies and research institutes that are involved in this field. The Tech building blocks where divided into three processes that occur within most 3D bio-priting processes: 1) Pre-processing/ Inputs 2) Processing/ Printing 3) Post-processing/ Outputs
- 9. Input ◦ Cells Large amounts need to be cultured for ‘bio-ink’ Viability pre-printing Maturation time needs to be known beforehand. ◦ Scaffold/support material Structural support to cells but has to be non-adherent. Biocompatible and easy to remove or biodegradable. ◦ CAD model/’Blue print’ High resolution of imaging. Easy of translation to printable model Mapping of multiple cell types.
- 10. Printing ◦ Resolution Micro/nano level accuracy ◦ Fast Prevents cellular differentiation from normal phenotype or worse cell death. ◦ Approach Mechanical forces of individual printing techniques ? Adverse effects ? ◦ Vasculature Provides cells with correct nutrition Output ◦ Maturation Culture time before its ready for implantation Analysis of whether it behaves the way it should ?
- 11. Research groups and companies were ranked from 1-5 (5 being the best) in each of the following categories: 1) Capacity Size of group Papers published Grants/ collaborations 2) Readiness What stage are they at with idea/research/product Data produced / experiments Clinical trials ? 3) Technology ‘coolness’ My subjective opinion on how novel their idea/research/approach is and whether it is commercially viable. Capacity x Rediness x ‘Coolness’ = Overall
- 12. 3D functional organ/tissueLithography based techniques 2-photon polymerisation (2PP) Normal approach (stereo) University of Vienna 3D Culture Bio printing 3D gels 1) Rice University, Institute AMRI 2) Sydney University, Dentistry Projection approach (mask image based) University of California, San Diego (UCSD). Magnetic Levitation Other Beads Rainbow Biosciences + n3D biosciences (company) Cronim 3D (company) University of Tokoyo, Institue of industiral sciences Cell Approaches No Cells Droplets Vesicle No Scaffold Scaffold University of Manchester, Derby group Oxybio (company) Layer by layer Droplets Inkjet approachLaser assisted Laser induced forward transfer Laser guided direct writing University of Bordeaux, Bioeng dept, TEAL group Solid Scaffold Soft Scaffold Rigid Scaffold Needle array Other Porous Scaffold Cell In-growth Other Non Porous Direct cell printing Cyfuse Biomedical (company) University of Manchester, Derby group University of Sydney, Eng & info technology University of Korea (POSTECH) Multiple Cells Single Cell University of Edinborough, Inst of Bio chemistry, biophysics and bioengineering 1) University of MIT, Regen Medicine 2) Skin Print (company) 5) University of Ediborough 6) University of Tokyo - Matsunga Group Ranking system (overall) : 1-34 = potential but early stage 25-68 = Ones to look out for 69 – 100 = Ones likely to excel 3) University of Vienna, Dept of additive manufacturing 1) Organovo (company) 2) Wake forest University – Regen Med - AFIRM 3) Rice University – Inst of AMRI 4) Harvard University – WYSS Institute Spheriods
- 14. Most advanced Regenerative Medicine centres in the world. Working on 3D printing of: ◦ Kidneys ◦ Liver ◦ Blood vessels ◦ Skin Collaborating with Armed Forces Institute of Regenerative Medicine ◦ Skin regen for burns victim ◦ Facial, skull, genital and Urinary reconstructions
- 15. Developing laser and UV light based 3D bioprinting techniques, including laser direct ablation, UV nanoimprinting, and dynamic optical projection stereolithography (DOPsL) for biomaterials.
- 16. Gained interesting information on 3D biopritning world. Experience working within a life sciences team, whom have many different portfolios. Complying huge amounts of information into small pieces of digestible information to relay on. Working with complete freedom to tackle a problem on my own. Reflective writing with Critical analysis.
- 17. And …. That im pretty good at 5Ks.
- 18. THANKS FOR LISTENING !
Editor's Notes
- #16: The biofabrication technique uses a computer projection system and precisely controlled micromirrors to shine light on a selected area of a solution containing photo-sensitive biopolymers and cells. This photo-induced solidification process forms one layer of solid structure at a time, but in a continuous fashion.