Bio Printing and Bio Inks
- 1. Presented by – Sushant B. Jadhav (18PBT206) Under the guidance of – Prof. Vandana B. Patravale 1
- 2. The aim of 3D bioprinting is to construct, in vitro, tissues, organs and other biological systems that mimic their native counterparts The materials used consist of natural and synthetic polymers, living cells, drugs, growth factors 3D bioprinting is an interdisciplinary field, requiring knowledge from developmental biology, stem cell science, chemistry, computer science, and materials science 2
- 3. Deciding application Selection of cells Selection of biomaterial Formulating bioink Deciding technique of bioprinting Fabrication process Computer program Bioprinting Validation and application 3
- 5. Design Factors and the capabilities of Bioprinting Design Considerations in Bioprinting Bioprinting Fabrication Procedures Methods to enhance Bioprinting 5
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- 7. 7 Shape and Resolution Material Heterogeneity 1. Heterogeneous Cells 2. Heterogeneous Biomaterials 3. Graduated Growth Factors Cellular-Material Remolding Dynamism
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- 11. Integrating Native and Engineered Tissue Coordinating Cell-Material Interaction Smart Biomaterials 1. Self-Assembly Materials 2. Stimulus Responsive Hydrogel Power Simulation 11
- 12. 12 4. Bio- inks Hydrogels Photocrosslin kable inks Thermo- responsive inks Microcarriers Cell aggregates Cell pellets Tissue spheroids Tissue strands Decellularize d matrix components
- 13. 13 Natural and naturally- derived polymers Synthetic polymers Gelatin Pluronics Collagen poly(N- isopropylacrylamide) (PNIPAAM) Elastin Fibroin Fibrin Alginate Hyaluronic acid (HA)
- 14. Functionalization for improving mechanical integrity Functionalization for improving printability Functionalization for enhancing biocompatibility and bioactivity 14
- 15. 15 Figure :Schematic summary of various methods of functionalization
- 16. Tissue engineering and regenerative medicine Transplantation and clinical applications Drug testing and high-throughput screening Cancer research 16
- 17. 7.1 Pluronic F127 hydrogel characterization and biofabrication in cellularized constructs for tissue engineering applications Emilia Gioffredi et. al. developed a method for printing cellularised scaffolds from thermosensitive hydrogels. 17 Figure : A, B) FEG-SEM micrographs of a four-layered hydrogel scaffold (needle Ø 200 μm, fiber spacing 600 μm); C, D) transversal sections of the fabricated fibers.
- 18. 7.2 Tailoring mechanical properties of decellularized extracellular matrix bioink by vitamin B2-induced photo- crosslinking Jinah Jang et. al. developed two-step process to solidified bio- ink. 18 Figure : Schematic illustration of a two-step crosslinking mechanism that applies concurrent crosslinking of vitamin-B2-induced covalent crosslinking and thermal crosslinking
- 19. Bioprinting is one of the promising technology expanding its horizon Integration of bioprinting techniques to enhance the printing process is a good strategy Bio-inks are core of bio-printing Different bio-inks with different bio-printing qualities thus we need to choose one according to our need There is no idle bio-ink for printing thus each bio-ink can be functionalized for increasing its property according to need of technique Synchronization of bioprinting and bioinks can definitely aid researchers to develop new applications of it 19
- 20. Gudapati H, Dey M, Ozbolat I., 2016, “A comprehensive review on droplet-based bioprinting: Past, present and future”, Volume 102, Pages 20-42. Ji Eun Kim, Soo Hyun Kim, Youngmee Jung, 2016, “Current status of three-dimensional printing inks for soft tissue regeneration”, Volume 13, Pages 636-646. Jia Min Lee and Wai Yee Yeong, 2016, “Design and Printing Strategies in 3D Bioprinting of Cell-Hydrogels: A Review”, Adv. Healthcare Mater., Volume 5, Pages 2556-2565. Ilze Donderwinkel, Jan C. M. van Hest and Neil R. Cameron, 2017, “Bio-inks for 3D bioprinting: recent advances and future prospects”, Polym. Chem., Volume 8, Pages 4451-4471. Ozbolat IT, Yu Y., 2013, “Bioprinting toward organ fabrication: challenges and future trends”, IEEE Trans Biomed Eng, Volume 60, Pages 691-699. 20
- 21. 21 THANK YOU