Lab 4
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Cell counts are important for various biological applications and medical purposes. They can provide information about health conditions and disease progression. There are several methods for counting cells, including using a square reticle on the microscope eyepiece, calculating the area of the microscope field of view, or using a digital microscope. The document then describes the steps for counting cells using a square reticle and calculating the number of cells within a given area using the microscope's field of view. Precise cell counts are necessary for studies of microorganism growth rates, cell viability experiments, and other applications requiring determination of cell concentration.
Lab 4
- 1. November 8, 2015 PRACTICAL CELL BIOLOGY LAB 4 Cell counting Introduction Cell counts may be used to identify diseases and to monitor a person's health. They can alert a doctor to an issue in a person's body and prompt a doctor to perform appropriate tests. Many biological applications such as microbiology, cell culture, blood work and many others that use cells require that we determine cell concentration for our experiment. Here are several examples for the need for cell counting: In medicine, the concentration of various blood cells, such as red blood cells and white blood cells, can give crucial information regarding the health situation of a person. Similarly, the concentration of bacteria in the blood or in other bodyfluids can reveal information about the progress of an infectious disease and about the degree of success ofthe immune system. Studies that examine the growth rate of microorganisms (in other words:how fast they divide to create new cells) require cell counting. Measurements of cell viability, i.e. measuring and calculating the fraction of dead and live cells from animal tissues such as liver, kidney……….etc, for example cells exposed to poison.
- 2. November 8, 2015 PRACTICAL CELL BIOLOGY LAB 4 Methods of cell counting from fixed tissue on slide 1. By using square which mounted on ocular lens. a) By using stage micrometer, find the length of one side of square on ocular lens. b) Find area of the square. Area of a square is given by: A = a2 Where a = length of side c) Remove stage micrometer and place a slide on the stage of microscope which you want to count number of cells on the slide. d) Count cells within squares of the ocular lens e) Find number of cells in one millimeter square.
- 3. November 8, 2015 PRACTICAL CELL BIOLOGY LAB 4 2. By using field of the microscope a) By using stage micrometer, find the diameter of the field of microscope. b) Find area of the field. Radius = diameter / 2 The area of a circle is π (Pi) times the Radius squared, which is written: A = π × r2 c) Remove stage micrometer and place a slide on the stage of microscope which you want to count number of cells on the slide. d) Count cells within field of the ocular lens. e) Find number of cells in one millimeter square. 3. By using digital microscope