Miledna All
This document summarizes four key milestones in the development of DNA technologies: 1. The first use of electrophoresis to separate hydrolyzed RNA molecules, laying the foundation for techniques to analyze nucleic acids. 2. The first construction of recombinant DNA molecules in vitro using restriction enzymes, DNA ligase, and bacterial plasmids, enabling new DNA technologies. 3. The development of fluorescence in situ hybridization (FISH) allowing detection and localization of specific DNA sequences within cells using fluorescently labeled probes. 4. The discovery of restriction enzymes that cut DNA at specific recognition sequences, enabling recombinant DNA techniques and genomic mapping. These discoveries transformed molecular biology.
