Pcr array guide
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This document provides a buyer's guide for RT2 Profiler PCR Arrays, listing the compatible plate formats and recommended RT2 qPCR Master Mixes for different real-time PCR instruments. It shows that PCR arrays are available in 96-well or 384-well formats to fit standard or fast block instruments from ABI, Bio-Rad, Roche, and others. The guide specifies the number of 96-well or 384-well PCR arrays that can be run with each master mix purchase. It also describes RT2 RNA QC PCR Arrays for validating RNA samples prior to gene expression analysis.
Pcr array guide
- 1. PCR A Find the correct plate format and PCR master mix for the instrument in your laboratory. Instrument Model PCR Array Plate Format A ABI 7500 FAST 96-well Block C ABI 7900HT Standard 96-well Block A ABI 7900HT FAST 96-well Block C ABI 7900HT 384-well Block E ABI 5700 (Perkin Elmer) A ABI 7700 (Perkin Elmer) A ABI StepOnePlus C iCycler A iQ5 A MyiQ A CFX96 D Chromo 4 (MJ Research) A Opticon (2) (MJ Research) Bio-Rad A ABI 7500 Standard 96-well Block D Mx3005p A Mx3000p A Mx4000 D LightCycler 480 96-well Block F LightCycler 480 384-well Block G Mastercycler ep realplex A Master Mix Catalog # A ABI 7300 Applied Biosystems ABI 7000 Eppendorf Roche Stratagene rrayS RT2 Profiler™ PCR Array Buyer’s Guide: Standard 96-well Pack Sizes 96 Genes & Controls per Plate Y = Plate Format: A, C, D, or F Master Mix Catalog # 2-Pack (PAXX-###Y-2) 2-Pack (PA-01#) 12-Pack (PAXX-###Y-12) 12-Pack (PA-01#-12) 24-Pack (PAXX-###Y-24) 24-Four Pack (PA-01#-24) Standard 384-well Pack Sizes 96 Genes & Controls Replicated Four Times per Plate Y = Plate Format: E or G 4-Pack (PAXX-###Y-4) XX, Y, ###, Master Mix Catalog # 4 x 2-Packs (4 x PA-01#) Species: HS for Human, MM for Mouse, RN for Rat PCR Array Plate Format - see table above Denotes Array Topic - see PCR Array list at: www.SABiosciences.com/ArrayList.php PA-012 PA-011 PA-010 PA-012 PA-010 Inquire - email support@superarray.net PCR Array 384 HT 384-well Pack Sizes 384 genes + controls per plate Y = Plate Format: E or G Master Mix Catalog # 2-Pack (PAXX-3###Y-2) 2 x 2-Packs (2 x PA-01#) 12-Pack (PAXX-3###Y-12) 2 x 12-Packs (PA-01#-12) 24-Pack (PAXX-3###Y-24) 2 x 24-Packs (2 x PA-01#-24) If you have questions about matching the plate format and master mix to your instrument, or if you don't see your 96-well or 384-well instrument in this buyer's guide, please contact Technical Support at 888-503-3187 or +1 301-682-9200 for assistance.
- 2. RT2 Profiler™ PCR Array Buyer’s Guide PCR Array Reagents Applied Biosystems and Stratagene PA-012 12 6 PA-012-12 12 PA-012-24 RT² SYBR Green / ROX qPCR Master Mix Bio-Rad 1 24 Master Mix Number of 384-well PCR Arrays Served 2 Instrument Type Number of 96-well PCR Arrays Served RT² SYBR Green / Fluorescein qPCR Master Mix Catalog # 2 PA-011 6 PA-011-12 24 Other 96-well Systems 1 12 12 PA-011-24 2 1 PA-010 12 6 PA-010-12 24 RT² SYBR Green qPCR Master Mix 12 PA-010-24 For high sensitivity and full functionality of the control features on the PCR Array: RT2 First Strand Kit 12 C-03 2 RT RNA QC PCR Arrays are available for testing the suitability of your RNAs for use with real-time PCR-based methods. RT2 RNA QC PCR Arrays Description Size Catalog # RT2 Human RNA QC PCR Array 1 PCR Array for quality control of twelve (12) Human RNA samples PAHS-999Y RT2 Mouse RNA QC PCR Array 1 PCR Array for quality control of twelve (12) Mouse RNA samples PAMM-999Y RT2 Rat RNA QC PCR Array 1 PCR Array for quality control of twelve (12) Rat RNA samples PARN-999Y Y, Plate Type: A, C, D, E, F, or G - see cover page See web site for over 100 PCR Array Pathways: www.SABiosciences.com/ArrayList.php © 2008 SABiosciences Corporation All Rights Reserved. RT2Profiler ™ PCR Array, is a trademark of SABiosciences Corporation. SYBR® is a registered trademark of Molecular Probes, Inc. ABI®, ROX® and StepOnePLus® are a registered trademarks of ® ® ® ® ® ® Applera Corporation. CFX96 , Opticon , Chromo4 , iQ5 , iCycler and MyiQ are registered trademarks of Bio-Rad Laboratories. Mx3000P®, MX3005P®, and Mx4000® are registered trademarks of Stratagene. TaqMan® and LightCycler® are registered trademarks of Roche Molecular Systems. Mastercycler® is a trademark of Eppendorf, Inc. Focus on Your Pathway using qRT-PCR SABiosciences Corporation 6951 Executive Way Frederick, MD 21703 www.SABiosciences.com T 301.682.9200 888.503.3187 F 301.682.7300 888.465.9859