protein microarray
Download as PPTX, PDF34 likes6,170 views
Protein microarrays are high-throughput methods that allow researchers to study protein interactions and functions on a large scale. There are three main types of protein microarrays: analytical microarrays use antibodies to detect specific proteins in samples; functional microarrays examine protein-protein and other molecular interactions; and reverse-phase protein microarrays profile protein expression levels and post-translational modifications by immobilizing cell or tissue lysates. Protein microarrays have applications in diagnostics, proteomics, studying protein functions, and analyzing antibodies.
protein microarray
- 2. Protein microarray A protein microarray (protein chip)is a high throughput method used to track the interactions & activities of proteins,& to determine their functions,& determining function on a large scale. Protein microarrays,are miniaturised and parallel assay systems that contain small amounts of purified proteins in a high density format.
- 3. Types of Protein Microarrays Protein Microarrays Analytical Protein Microarray Functional Protein Microarray Reverse Phase protein Microarray
- 4. Analytical microarrays(or antibody microarrays) have antibodies arrays on solid surface,and are used to detect proteins in biological samples. Often a second is used to detect a protein that is captured by the antibody attached to the solid phase,in a principle similar to that of sandwich immunoassay, in which the first antibody is spotted on the array and then a captured antigen on the chip is detected with a second antibody that recognises a different part of antigen. Analytical Protein Microarray
- 5. Cont.....
- 6. Text Functional Protein Microarray Functional protein microarrays have recently been applied to many aspects of discovery based biology,including protein-protein,protein-lipid,protein-DNA,protein drug,&protein–peptide iinteractions. These can be used to identify enzyme substrates. These can also be used to detect antibodies in a biological specimen to profile an immune response.
- 7. Cont.....
- 8. Reverse Phase Protein Microarray In RPA, cellular or tissue lysates are immobilized on the slides. RPAs allow for the determination of the presence of altered proteins or other agents that may be the result of disease. Specifically, post translation modifications,which are typically altered as a result of disease can be detected using RPAs.
- 9. Applicaton There are five major areas where protein arrays are being applied- • diagnostics. • proteomics. • protein functional analysis. • antibody characterisation & treatment.