Proteomics presentation
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The document presents information on Fourier Transform Ion Cyclotron Resonance (FTICR), including that it was invented in 1974, uses magnetic and electric fields to determine ion mass-to-charge ratios based on cyclotron frequency, and has high resolution applications in fields like proteomics and isotopic analysis but also high costs.
Proteomics presentation
- 1. ESSORE UNIVERSITY OF SCIENCE & TECHNOLOGY A PRESENTATION ON Fourier Transform Ion Cyclotron Resonance Presented by MD ROBEL AHMED ROLL NO: 14 06 03 4TH YEAR 2ND SEMESTER DEPT. OF GENETIC ENGINEERING AND BIOTECHNYLOGY Presented to DR. MD. MASHIAR RAHMAN ASSISTANT PROFESSOR DEPT. OF GENETIC ENGINEERING AND BIOTECHNYLOGY DATE OF SUBMISSION: 15 SEPTEMBER 2018
- 3. INDEX History of invention Fourier Transform Cyclotron mechanism Principle Basic structure of the analyzer FTICR Mechanism Advantages & Disadvantages Applications References
- 4. HISTORY OF INVENTION Invented by Melvin B Comisarow and Alan G Marshall at the UNIVERSITY OF BRITISH COLUMBIA. First publication done in 1974 on the journal on chemical physics letter.
- 5. CYCLOTRON MECHANISM Magnetic field Electric field
- 7. Types of mass analyzer determining mass to charge ratio of ion It is based on cyclotron frequency of the ion in a fixed magnetic field Ions are trapped in a penning trap where they are excited(based on cyclotron frequency. While exciting they converted to larger cyclotron radius by an oscillating electric field orthogonal to magnetic field. After the removal the excitation field, ions are rotating at their cyclotron frequency (as a packet PRINCIPLE
- 8. BASIC STRUCTURE OF THE ANALYZER
- 9. Radio frequency source Data output Detector plate resistor amplifier ions Excitation plate RF FTICR MECHANISM
- 10. ANALYSIS OF THE RESULT Ѡ= 𝑞𝐵 𝑚 1 f = 𝑞𝐵 2¶𝑚 2 ESI FT-ICR mass spectrum of 20-|M ubiquitin in an aqueous 10-mM ammonium acetate solution, measured in terms of relative intensity versus m/z.
- 11. ADVANTAGES OF FTICR DISADVANTAGES OF FTICR High Accuracy Electrospray ionization Mass accuracy Extreme high resolution Costly(expensive at $1-2million for a standard instrument) Operation require highly skilled personnel
- 12. FIELD OF IMPLEMENTATION • High resolution is also useful in studying large macromolecules such as proteins with multiple charges, • Isotope analysis • Useful in analyzing phosphorylation states, O- or n-linked glycosylation, and sulfating