QT-Quick_Testing
- 1. “Half of All Covid Tests Are Positive in Mexico, Highest in the World” - Bloomberg 01.07.2020
- 2. The Latin American nation has stubbornly shunned wide-scale testing and instead runs exams only on the sickest of patients. Deputy Health Minister Hugo Lopez Gatell in late May said anything more would be “a waste of time, effort and resources.” - Bloomberg Source: https://www.bloomberg.com/news/articles/2020-07-01/half-of-all-covid-tests-are-positive-in-mexico-highest-in-world
- 3. https://coronavirus.jhu.edu/data/new-cases The first case of COVID-19 in Mexico was reported 129 days ago on 28.2.2020. Since then, the country has reported 252.165 cases, and 30.366 deaths.
- 4. It is evident, that in order to reopen their economies, countries must increase their testing capabilities. That is why we bring you ITQT - ISOTHERMAL QUICK TESTING. A COVID-19 testing process that is cheaper and faster than common RT-PCR Tests.
- 5. THE TEAM QT 1: NIKOLINA Field expert She brings the needed expertise in the Biochem environment QT 2: RALPH Networker Establishes connections to people in the right places to spread our message
- 6. UnternehmerTUM GmbH / 21.11.19 The IT-QT guideline provides detailed information about the steps displayed below to test for COVID-19 https://international.neb.com/protocols/2016/08/15/warmstart-colorimetric-lamp-2x-master-mix-typical-lamp-protocol-m1800 http://oxgen.ge/product/pcr-fragment-purification/ 0. Sample collection by medical staff. The patient sample is taken with a swab, packaged and labelled. Patient data is stored with a smartphone. QR code labels are used throughout the process. 1. Virus RNA extraction 2. QPCR RNA amplification is done with a reverse transcription and TUM certified isothermal LAMP molecular biology test kits in 30 mins using a heatblock instead of a thermocycler 3. machine result readout is ready after 1h with minimal hands on time. QR codes link the results to the patient data. Documentation happens by smartphone photo and is stored offline. Positive results should be confirmed with QPCR
- 7. But how does the LAMP processes compare to current manual procedures? RT-QPCR ● Bulky and cumbersome ● Specialised thermal cycles required (high demand) ● 4-8 h until result ● Requires skilled technicians ● Requires an additional RT-step ● Unstable reactions prone to inhibitors requiring purification steps ● Detects DNA ● Tested on patient samples LAMP ● Smaller, simpler, portable ● Only a heat block is required ● 1h until result ● Requires no specific skill ● can be performed directly on RNA ● Stable and inhibitors are tolerated, and thus purification steps are not required ● Detects DNA and RNA ● Less tested on patient samples
- 8. With the IT-QT guideline we hope to enable countries, to increase their testing capabilities and to become independent from scarce resources from foreign countries. This will enable them, to start controlling the COVID-19 pandemic and to reopen their economies in a safe way
- 9. Next Steps July 2020 Deliver Guideline for LAMP Process September 2020 Deliver Guideline to produce reagents independently October 2020 Secure funding to improve processes using lean production methods November 2020 Release guideline for optimal mobile lab construction JUL AUG SEP OKT NOV DEC August 2020 Establish network and launch website to promote our content
- 10. Appendix
- 11. UnternehmerTUM GmbH / 21.11.19 Micro.bio Lab.bio Safely isolate and extract coronaviruses using a mobile glovebox with negative pressure and autoclave to sterilize contaminated materials. The station is designed for SARS- Cov-2 RNA extraction and comes with a RNA extraction kit supply by Oxgen company. The station turns any tent, airport, or hospital room or any place you need into a fully functional biosafety level 2 facility. The station provides a heat block, pipettes, a fridge and freezer to keep your reagents fresh. The isothermal reaction can be controlled in temperature and duration with the heatblock. Diagnostic results are documented by a photo with the users smartphone. The convenient storage of consumables and the safe transportation of chemicals make it the ideal mobile lab platform for any environment. Our Bio.Kitchen Mobile Lab covers all processes for COVID-19 detection and safe sample handling UnternehmerTUM GmbH / 21.11.19 11 https://www.chaibio.com/openqpcr http://www.glovebox-systemtechnik.de/GS- Kunststoff-Glovebox.php all pictures are for illustration only. The final product may differ.