Techchallenge Covid-19 & Bio.Game
- 1. Tech Challenge Bio.Kitchen Challenges 1. Teach COVID a Lesson: AR guided COVID-19 Testing in a mobile Lab 2. Bio.Game: Build Tools to fast forward Evolution 23.04.2020
- 2. ootstrapping im Bio/Life-Scince Bereich “If I were a teenager today, I'd be hacking biology.” Bill Gates Bootstrapping biological innovation
- 3. Bio.Kitchen enables lean and agile development of life science innovation Biological innovation to date is not agile. Large investments and time is required to develop new prototypes until market readiness. Our mobile Bio.Kitchen brings agile innovation into the domains of chemistry, biology, pharma and medicine. In our courses we merge the organic, technical and digital worlds to generate sustainable generation of value following the UN “one health” concept, to give answers to the challenge of the 21. century: green economy, food and healthcare. Agility: We analyze technology, user needs and viability in an integrated and flexible process Transdisciplinarity: We work across disciplines High-Tech Hacking: We hack and recombine technologies creatively Rapid Prototyping: we can quickly iterate and test biological, hardware and software prototypes in less than a week Neutral Ground: We dont take any equity in our users inventions and open source our own technology Founder’s Mindset: We enable holistic education and personality development.
- 4. Bio.Kitchen is an open community lab for tech-talents and life science innovation our workstations: Lab.Bio - Micro.Bio - Syn.Bio - Play.Bio our service: biopreneur community - biotech hacking - open laboratory - educational courses Rüdiger Trojok Lab Lead & Biohacker Leo Sprengel Lab Assistant Dr. Dominik Böhler Head of Entrepreneurship & Tech Education
- 5. with our own mobile Tools & Tech we teach rapid prototyping & agile Innovation Open Source lab technology High-Tech Analytics Lab on a Chip Chemistry and Biology open reagents collection We lower administrative, logistic and regulatory hurdles to enable agile innovation in the life sciences. Our competent team gives practical workshops and provides a full equipped mobile lab infrastructure for chemistry, biology, medtech and material sciences. Participants In our workshops will be guided from idea to a functional prototype and then receive further coaching on how to form a startup and commercialize their ideas and technologies.
- 7. Biocommons Synbio Oath Read & Write DNA printing DNA Biological Transformation cradle to cradle economics Personalised Medicine phage therapy lectures Play.Bio SLA 3D printing Lego Biochips Future lab Bio-Computer workshop Biohacking pharma bioethics seminar biological rapid prototyping Syn.bio cell free system workshop Biosensors station Internet of Living Things workshop Lab.Bio fungi cultivation plant cultivation insect cultivation Microbiology for beginners Micro.Bio sequencing DNA Bio.Kitchen lab intro workshop yeast cultivation open source open licensing of high tech Biotic games bio tournament Biohacking workshops hacking evolution workshop prototype nature biodesign studies Microfluidics DIY automation workshop sci-tech concepts education format bootstrapping biotech startups personal phage therapy synthetic phage workshop advanced Microbiology Bio-Playstation workshop Copying DNA workshop one health strategy interplanetary Bioteleportation bacteria cultivation phage cultivation We offer a large range of technology workshops, materials and tools
- 8. Micro.bio Syn.bio mini & max Play.bio Lab.bio Safely grow, identify and select microorganisms using a mobile incubator and autoclave. Expression of transgenic proteins is possible with cell free systems. Syn.bio mini provides DNA analytic hardware. The Syn.Bio max station provides the Kilobaser and Nanopore technologies for sequencing and synthesis of DNA of DNA and comes with a computer to program and analyze DNA with a QPCR and our desktop tools. Our microfluidic toolbox enables you to 3D print microfluidic devices. Combine detectors for optical density measurement with actuators like pumps and valves and our open source fluidic bricks to rapid prototype your own Bio.Tournament setup. The station is equipped with a centrifuge, pipettes, a transportable fridge and freezer to keep your reagents fresh and usable anywhere. The convenient storage of consumables and the safe transportation of chemicals make it the ideal mobile lab platform. Our Bio.Kitchen Mobile Lab covers the most relevant processes for early-stage biological rapid prototyping within four work areas.
- 9. Our Bio.Kitchen Mobile Lab is a lab-in-a-box for events, education and innovation We developed four mobile laboratory stations: Lab.bio (comprised of 2 units), Micro.bio, Syn.bio and Play.bio, which can be easily equipped, transported and operated with minimal logistic effort. The mobile lab is ready to use within 24h, anywhere you want it to be. The full range of our courses can be offered at any preferred location. The lab storage solution is optimized for our equipment and courses (see previous slide) and is size compatible with Eurocontainers. Extensions of the table area can be added and stored conveniently for transportation. We offer new demonstration experiments and the Bio.Tournament program. Of course, the lab itself can be used independently as a technology support unit for any desired application, too.
- 10. COVID-19 Dance - North Africa lacks testing capacity and needs a simple test protocol. COVID-19 Dance consortium is building solution and independent supply chain for COVID-19 Testing in mobile labs using PT-qPCR and isothermal amplification. Consortial Lead: Concept Development and Project Management Production and manufacturing of mobile lab hardware. OxGEn produces RNA extraction reactions and gets validation by Georgian CDC Clinical pilot with diagnostic labs in May Pilot study to establish additional test capacity - Production of COVID-19 isothermal amplification - 30 mobile lab stations - 1.6 Million Tests in 6 months pilot study - Scalable technology for continental rollout - Sustainable reuse of technology after pandemic Patient sample Virus RNA Rapid Virus RNA extraction ~30min Isothermal Ampflication ~30min Reagents 1h Patient Diagnosis Northern Africa needs an immense increase in test capacity to fight off potential effects of COVID-19 Mai 2020 - Continuous improvement, field testing and education of healthcare professionals - December 2021 TUM produces a batch of isothermal amplification kits.
- 11. UnternehmerTUM GmbH / 21.11.19 AP5: Skalierung und Betrieb vor Ort AP4: Produktion von Test-Kits AP1: Produktion mobiler Labore AP2: Ausbildung von Trainern 8 200 ~35€ 30 300 25-30€ 30 800 <25€ # Labore Tests/Tag und Labor Kosten/Test In 3 Phasen können wir Testkapazität für bis zu 1.6 Millionen Tests bei Vollkosten <25€ pro Test schaffen. ● Bau von 8 Stationen ● Zukauf von Reagenzien und Maschinen für qPCR-Verfahren ● Probebetrieb von 8 mobilen Laboren vor Ort ● Erprobung Logistik April-Mai ‘20 Prototyping Juni-Juli ‘20 Testbetrieb, Produktion Reagenzien August-Dezember ‘20 Skalierung Betrieb vor Ort Bau und Betrieb Labore Entwicklung und Produktion Reagenzien KPIs AP3: Entwicklung und Validierung von vereinfachten Testverfahren: ● Optimierung oder Effizienzsteigerung der isothermalen Amplifikationsreaktion ● Klinische Vorstudie am MRI ● Selektion eines Produktionspartners für Test-Kits ● Schrittweise Skalierung auf 1.5 Millionen Kits ● Ausbildung von 12 Trainern ● Ausbildung von weiteren 24 Trainern ● Entwicklung Online-Content ● Produktion von qPCR Testkits mit OxGen, Tiflis, Georgien ● Bau von weiteren 22 Stationen ● Skalierung der isothermalen Reaktion ● Remoteunterstützung bei Bedarf ● Optimierung der Testkits auf den Einsatz in Nordafrika auf Basis der Forschungsergebnisse ● Kontinuierliche Produktion und Logistik
- 12. Enabling Corona Testing Stations
- 13. UnternehmerTUM GmbH / 21.11.19 Phase 1: qPCR test protocol give high quality results in 2h in the mobile lab https://www.chaibio.com/openqpcr 0. Sample collection by medical staff. The patient sample is taken with a swab, packaged and labelled. Patient data is stored with a smartphone. 1. Virus RNA extraction virus lysis and RNA purification using established protocols with a centrifuge in 1h. QR code labels are used throughout the process. 2. QPCR RNA amplification is done with a reverse transcription and QPCR protocol and CE certified molecular biology test kits in 2h. 3. machine result readout is ready after 2h. QR codes link the results to the patient data. Documentation happens by smartphone photo and is stored offline.
- 14. UnternehmerTUM GmbH / 21.11.19 Phase 2: quick-pre-test protocol give results in 1h without the need for thermocyclers or centrifuges https://international.neb.com/protocols/2016/08/15/warmstart-colorimetric-lamp-2x-master-mix-typical-lamp-protocol-m1800 http://oxgen.ge/product/pcr-fragment-purification/ 0. Sample collection by medical staff. The patient sample is taken with a swab, packaged and labelled. Patient data is stored with a smartphone. 1. Virus RNA extraction using a simplified virus lysis and RNA purification protocol in 30 mins. QR code labels are used throughout the process. 2. QPCR RNA amplification is done with a reverse transcription and TUM certified isothermal LAMP molecular biology test kits in 30mins. 3. machine result readout is ready after 1h with minimal hands on time. QR codes link the results to the patient data. Documentation happens by smartphone photo and is stored offline. Positive results should be confirmed with QPCR
- 15. UnternehmerTUM GmbH / 21.11.19 Phase 3: quick-pre-test protocol give results in 20mins without the need for thermocyclers or centrifuges https://international.neb.com/protocols/2016/08/15/warmstart-colorimetric-lamp-2x-master-mix-typical-lamp-protocol-m1800 http://oxgen.ge/product/pcr-fragment-purification/ 0. Sample collection by medical staff The patient sample is taken with a swab, packaged and labelled. Patient data is stored with a smartphone. 1. Isothermal RNA amplification is done with LAMP molecular biology test kit in a manual but high throughput method. 2. visual result detection is ready after 1h for photo documentation with minimal hands on time and lab equipment. Positive results should be confirmed with qPCR.
- 16. UnternehmerTUM GmbH / 21.11.19 Phase 4: automated quick-pre-test protocol give results with only 5 mins hands on time https://international.neb.com/protocols/2016/08/15/warmstart-colorimetric-lamp-2x-master-mix-typical-lamp-protocol-m1800 http://oxgen.ge/product/pcr-fragment-purification/ 0. Sample collection by medical staff The patient sample is taken with a swab, packaged and labelled. Patient data is stored by any state approved smartphone app for Corona pandemic control. 1. automated Isothermal RNA amplification is done with LAMP molecular biology test kit in a automated and high throughput method. No further lab equipment needed. 2. visual result detection is ready after 1h with 5mins hands on time. Results are automatically uploaded to any Corona pandemic control app.
- 17. UnternehmerTUM GmbH / 21.11.19 Micro.bio Lab.bio Safely isolate and extract coronaviruses using a mobile glovebox with underpressure and autoclave to sterilize contaminated materials. The station is designed for COVID-19 RNA extraction and comes with a RNA extraction kit supply by Oxgen company. The station turns any tent, airport, or hospital room or any place you need into a fully functional biosafety level 2 facility. The station provides a heat block, pipettes, a fridge and freezer to keep your reagents fresh. With a heatblock, the isothermal reaction can be controlled in temperature and duration. Diagnostic results are documented by a foto with the users smartphone. The convenient storage of consumables and the safe transportation of chemicals make it the ideal mobile lab platform for any environment. Our Bio.Kitchen Mobile Lab covers all processes for COVID-19 detection and safe sample handling UnternehmerTUM GmbH / 21.11.19 17 https://www.chaibio.com/openqpcr http://www.glovebox-systemtechnik.de/GS-Kunststoff-Glovebox.php all pictures are for illustration only. The final product may differ.
- 18. Our Bio.Kitchen Mobile Corona Diagnostic Lab is a ruggedized lab-in-a-box for pandemic response ▪ 3 mobile lab stations: Lab.bio (2 units), Micro.bio, Syn.bio (optional) ▪ Easily transported and operation - ready to use within ~24h, anywhere in EU ▪ Compatible to our molecular and microbiological lab tools ▪ Optimized equipment storage compatible with Eurocontainers ▪ Extendable table top area to a maximum of 7.2 m² (5 work spaces) ▪ On demand: mobile energy generator and voltage regulators
- 19. UnternehmerTUM GmbH / 21.11.19 With 1 station per 100 000 citizens our mobile lab allows to compensate the lack of testing capacity in Germany. Assumptions: ● Staffing: 3 clinical laboratory technician working in shifts 24/7 ● 36 samples handled per hour ● with QPCR testing hands on time 120 mins to result ● isothermal testing hands on time 60 mins to result ● with automation the diagnoses per day can be scaled significantly Maximum manual capacity in Phase 3: 800 diagnoses per station per 24h
- 20. Play.Bio Bio.Game Environmental Challenge - evolve your organisms to live in the microplastic habitat Bio.Game teams develop biotechnological innovations and playfully compete with each other by growing their organisms to explore a new habitat on a petri dish. Participants learn our unique rapid prototyping methodology to hack ecology and evolution to outsmart your opponent bacterial cultures. By building their own open source lab-on-a-chip “bio playstation” teams can test their ideas in a real-time biological competition against other teams. The Bio.Game can be hosted using our Micro.Bio and Play.Bio mobile lab stations. Help us to develop this biological gaming station. Lab.bio ● Evolve ● Select ● Expression Micro.Bio ● Cultivate ● Optimize ● Diversify Play.bio ● Automate ● Miniaturise ● Scale up play.bio
- 21. 21 Challenge 2: Bio-Tournament 21 A biological competition. Hack evolution and outsmart your opponent bacterial cultures. Advance your microfluidic workstation and overcome the opponents cells with phages and resistance breeding. Apply your setup in a real-time biological competition to take over as much space on the agar playground as possible.
- 22. Moore’s Law for Biology Exponentially grow the number of experiments per scientist Experiments per day 10.000.000 1.000.000 100.000 1.000 100 2009 2012 2015 2018 2021 2024
- 23. 23
- 24. Bio.Kitchen prices and offers ▪ take part in online and lab courses - mobile lab construction workshop - Micro-bio for beginners course - Corona diagnosis course - Rapid Prototyping Lab Automation course ▪ use our open innovation lab during this semester for free every monday afternoon ▪ receive free reagents and play the Bio.Game against the Bio.Kitchen team ▪ be a part in the first trial run of the mobile COVID-19 test lab Join our Deep Dive on Wednesday 1pm - 3pm on Zoom to get more information or contact me on Slack any time. Come by on monday afternoon to our Laboratory in: UnternehmerTUM GmbH Lichtenbergstr. 6 85748 Garching (Munich), Germany
- 25. Vielen Dank! UnternehmerTUM GmbH Lichtenbergstrasse 6 85748 Garching (Munich), Germany Tel.+49 (89) 18 94 69-0 www.unternehmertum.de UnternehmerTUM GmbH Lichtenbergstr. 6 85748 Garching (Munich), Germany +49-178-8835667 +995 598 26 67 61 trojok@unternehmertum.de Rüdiger Trojok Lab Lead & Biohacker