Reforestation through micropropagation
- 1. REFORESTATION THROUGH MICROPROPAGATION By Harish Kumar S M.Tech-Biotechnology
- 2. Microprogation • Budding shoots are culture inside test tubes & nourished on an agar gel with growth regulators to coax them into multiplying secondary shoots. • Then it transplanted into soil, these shoots eventually root and become whole, free-living plantlets that can be put in the field.
- 3. 1. Meristem culture 2. Callus culture 3. Suspension culture 4. Embryo culture Methods
- 7. Steps involved in micropropagation
- 8. Reforestation by micropropagation • Forest trees are renewable source of food, fuel wood, timber and other valuable non-timber products. • The ever increasing human & livestock populations demand for plant products; resulting in over exploitation of forest trees. • There is an urgent need for conservations of germplasm and also for propagation of a sustainable utilization of forest trees. • Micropropagation of tree species offers a rapid means of producing clonal planting stock for afforestation, woody biomass production & conservation of rare germplasm. • Therefore millions of plant species can be maintained inside culture vials in a small room and this is the suitable alternative to conventional methods of vegetative propagation of plants.
- 9. Factors affecting micropropagation • Genotype of the plant. • Physiological status of the explants. • Culture media. • Culture Environment -Light, temperature and composition of gas phase
- 10. Advantage • Able to produce many plants that are clone of each other. • Can able to produce disease free plants. • It is the only viable method of regenerating genetically modified cells or cells after protoplast fusion. • Greater number of plants can be produced per square meter and the propagules can be stored longer in a smaller area.
- 11. Disadvantage • High chances of contamination • Infected plant sample can produce infected progeny • Not all plant can be successfully micropropagated. • Some plants are very difficult to disinfect fungal organism • Genetic variation will be low
- 12. Application • High rate of plant propagation • Production of disease-free plants • Production of seeds in some crops • Automated micropropagation
- 13. Thank you