Selective Media
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This document discusses selective media used for bacterial growth. Selective media enhance the growth of particular bacteria while inhibiting other organisms. Some examples of selective media include Eosin Methylene Blue (EMB) agar, which selects for Gram-negative bacteria; MacConkey agar, which selects for lactose-fermenting bacteria; Mannitol salt agar, which selects for staphylococci; and Phenyl Ethyl Alcohol (PEA) agar, which inhibits most Gram-negative bacteria. Each type of selective media exploits the nutritional requirements or sensitivities of different classes of bacteria.
Selective Media
- 1. Bangabandhu Sheikh Mujibur Rahman Science & Technology University Md. Golam Rabbani Student ID: 16PHR052 Department of Pharmacy BSMRSTU Topic: Selective Media for Bacterial Growth
- 3. Contents Contents ďś Bacteriological Media ďś Types of Media ďś Selective Media ďś Types of Selective Media ďEosin Methylene Blue(EMB) agar based Selective Media ď MacConkey agar based Selective Media ď Mannitol salt agar based selective Media ď Phenyl Ethyl Alcohol (PEA) agar based Selective Media
- 4. ďIn bacteriological medium where describe, discuss, analyze about nutritional requirements for bacterial growth, cultivation and reproduction. Bacteriological Media
- 5. Types of Media ďśSelective Media ďśDifferential Media ďśAssay Media ďśMedia for Enumeration of bacteria ďśMedia for Characterization of Bacteria ďśMaintenance Media ďśSolid and Semisolid Media
- 6. Selective Media ďThese media provide nutrients that enhance the growth and predominance of a particular type of bacterium but do not enhance other types of organism that may be present.
- 7. Types of Selective Media There are some types of selective media- ďEosin Methylene Blue(EMB) agar based Selective Media ďMacConkey agar based Selective Media ďMannitol salt agar based selective Media ď Phenyl Ethyl Alcohol (PEA) agar based Selective Media
- 8. EMB agar based Selective Media ďśEosin Methylene Blue agar (EMB agar) contains the dyes eosin and methylene blue. They inhibit Gram-positive organisms. Such a medium is selective for Gram-negative species. Lactose-fermenting organisms such as S. enterica produce a black precipitate on EMB. http://www2.highlands.edu/academics/d ivisions/scipe/biology/labs/rome/selecti vedifferential.htm
- 9. MacConkey agar based Selective Media ďś MacConkey agar is similar to EMB agar in that it is also selective for Gram-negative species and differential with respect to lactose fermentation. MacConkey agar is used for the detection of coliforms and enteric pathogens based on their ability to ferment lactose. http://www2.highlands.edu/academi cs/divisions/scipe/biology/labs/rom e/selectivedifferential.htm
- 10. Mannitol agar based Selective Media ďśMannitol salt agar (7.5% NaCl) medium is a selective for staphylococci and differential with respect to mannitol fermentation. Growth of most bacteria without staphylococci, is inhibited by the high concentration of salt in the medium. http://www2.highlands.edu/academics/d ivisions/scipe/biology/labs/rome/selecti vedifferential.htm
- 11. PEA agar based Selective Media ďśPhenyl ethyl Alcohol (PEA) agar is a selective medium which inhibits the growth of most Gram negative organisms. Phenyl ethyl alcohol acts to disrupt lipid structure in the membrane of Gram negative organisms as well as inhibiting protein synthesis. http://www2.highlands.edu/academics/d ivisions/scipe/biology/labs/rome/selecti vedifferential.htm