![18
Vanquish Horizon Delivers Method Speed-Up
Standard Method Sequence Coverage
100%
100%
100%
100%
100%
Acclaim 120 C18 column, 2.1 x 250 mm, 2 - 45% ACN + 0.1% FA, 60℃.
5 10 15 20 25 [min]
Time (min)
14.64
20.23
10.592.73
10.78
14.52
7.99
2.70
6.97 9.43
5.20
1.86
4.45
6.02
3.35
1.13
3.12
4.12
2.43
1.08
30 min gradient
0.4 mL min-1
600 bar
20 min gradient
0.4 mL min-1
600 bar
13 min gradient
0.6 mL min-1
850 bar
8 min gradient
1.0 mL min-1
1150 bar
5 min gradient
1.1 mL min-1
1300 bar
• Vanquish used for high
throughput peptide mapping
• Gradient time reduced from 3 to
5 min with constant 100%
sequence coverage
• Thermo Scientific™ Q
Exactive™ HF hybrid
quadrupole-Orbitrap mass
spectrometer delivers enough
data points per
chromatographic peak
• Vanquish is able to speed up
peptide mapping significantly](https://image.slidesharecdn.com/lctouroverview-190214161716/85/Separate-Your-Science-LC-tour-introduction-17-320.jpg)
![20 Proprietary & Confidential
Experimental Results – Pre-Compression
Column: Hypersil Gold, 1.9 µm, 2.1 × 100 mm
System: Vanquish System
Mobile Phase: A–water
B–acetonitrile
Gradient:
Flow rate: 260 L/min
Pressure: 300 bar (max.)
Comp. Vol: 0 µL and 3 µL
Temperature: 25 ºC
Injection: 1 µL
Detection: pump pressure sensor
Time
[min]
% A %B
0 60 40
0.4 60 40
4.0 0 100
4.8 0 100
4.9 60 40
10.0 60 40
w/o pre-
compression
w/ pre-
compression
Inject command at 0.1 min to track the pressure drop
isocratic step from 0 to 0.4 min (cycle completed)
No pressure drop during injection
Why is this important?
• Reduced physical stress on column longer lifetime w/o
performance loss less work
• More stable flow in early gradient forming better retention time
reproducibility better results more confidence](https://image.slidesharecdn.com/lctouroverview-190214161716/85/Separate-Your-Science-LC-tour-introduction-19-320.jpg)
Separate Your Science LC tour introduction
- 1. The world leader in serving scienceProprietary & Confidential Separate Your Science Tour Oskari Aro (a.k.a. Tuomo Lukkari) 2019
- 2. 2 Proprietary & Confidential Agenda 9:00 Thermo Scientific liquid chromatography solutions Oskari Aro 9:30 Smarter workflows with Chromeleon Merja Castrén 10:00 Charged Aerosol Detectors - What to try after UV and MS fail? Jan Pettersson 10:30 UV and DAD detectors - an in-depth overview Jan Pettersson 11:10 Coffee 11:30 Column selectivity Naghmeh Berglund 11:50 Enhanced bioseparations for peptide mapping and Mabs Naghmeh Berglund 12:10 Lunch 13:00 How solvent quality affects your analysis Jan Pettersson 13:20 Advanced detection with mass spectrometry Tuomo Lukkari 13:50 Carbohydrates analysis Jan Pettersson 14:30 Tips and tricks for HPLC Jan Pettersson 15:10 Chat with experts, Vanquish HPLC demo 16:00 Close
- 3. 3 Proprietary & Confidential We enable our customers to make the world healthier, cleaner and safer. A Mission We are Proud of
- 4. 4 Proprietary & Confidential • ~$21 billion in revenues • >$900 million spent on R&D • >60,000 employees • 50 countries • 5 premier brands The World Leader in Serving Science
- 5. 5 Proprietary & Confidential Complementary Segments Create Competitive Advantage Analytical Instruments Life Sciences Solutions Laboratory Products and Services Specialty Diagnostics 27% 17% 22% 34%Microbiology Antimicrobial Susceptibility Testing Solutions QuantStudio Dx R qPCR Genetic Sciences BioPharma Services Laboratory Chemicals Bioproduction Cell Culture Reagents Enterprise and Instrument Services Biosciences Life Science Reagents Pathology Rotary Microtome Mass Spectrometry Fusion Lumos MS Chromatography Vanquish UHPLC Electron Microscopy Titan Krios TEM Reproductive Health Microarrays iCAP Triple Quad MS Clinical Diagnostics PCT Biomarkers Lab Equipment TSX ULT Freezers ImmunoDiagnostics ImmunoCAP Allergy and EliA Autoimmunity Tests ~$21B Revenue NOTE: Revenue and segment percentages based on LTM through Q1 2017. SeqStudio CE System Lab Consumables E1 ClipTip Pipette System Clinical Oncology Next-Gen Sequencing Transplant Diagnostics NXType High-Resolution Genotyping Chemical Analysis Gemini Handheld Analyzer
- 6. 6 Proprietary & Confidential Innovation in Orbitrap Technology Next Generation GC-MS Orbitrap™: Ten Years Young
- 7. 7 Proprietary & Confidential Increased flexibility in liquid chromatography Vanquish DUO UHPLC System Chromeleon 7.2 SMART Digest Kit MAbPac Columns Delivers exceptional performance, reliability and reproducibility Three workflows. Two flow paths. One integrated UHPLC solution. Complemented by kits, software and columns Thermo Scientific ISQ EC Mass Spec
- 8. 8 Proprietary & Confidential 40+ years of innovation in Ion Chromatography Thermo Scientific Dionex Ion Chromatography Portfolio: Peace of Mind Every Day Thermo Scientific Dionex Aquion IC System Thermo Scientific Dionex Integrion HPIC system Thermo Scientific Dionex ICS-4000 Thermo Scientific Dionex ICS-6000 HPIC Thermo Scientific ISQ EC Mass Spec
- 9. 9 Proprietary & Confidential Sample Prep, Kits and Consumables • MS Crosslinking reagents • Capillary flow HPLC columns • Metabolomics Standards Kits Software Instruments Orbitrap HRAM MS • Small molecule analysis • Protein analysis • Cloud Applications • Spectral Libraries Mass Spec Innovations in 2018 Triple Quadrupole LC-MS Orbitrap Fusion™ Lumos™ MS – Options TSQ Altis™ MS TSQ Quantis™ MS Q Exactive™ HF-X LC-MS Proteome Discoverer™ Software Compound Discoverer™ Software Biocrates AbsoluteIDQ p400HR Kit DSSO and DSBU MS-Cleavable Linkers EASY-Spray™ PepMap 150µm Column • UVPD • 1M • APD Food and Beverage Environmental Translational Research/ Precision Medicine Life Science Research “omics” Biopharmaceuticals Exactive™ GC Orbitrap™ GC-MS TSQ 9000 GC-MSISQ 7000 GC-MS
- 10. 10 Proprietary & Confidential NEW! Thermo Scientific ISQ EC/EM Single Quadrupole Mass Spectrometers • Mass range 10-1250 m/z • HESI only • Perfect for IC-MS or LC-MS • For ion chromatography (IC-MS) and liquid chromatography (LC-MS) • Built for routine analysis and long-term signal stability • Controlled by Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS) Thermo Scientific™ ISQ™ EC Single Quadruple Mass Spectrometer • Mass range 10-2000 m/z • HESI and APCI Options • Perfect for LC-MS Thermo Scientific™ ISQ™ EM Single Quadruple Mass Spectrometer
- 11. 12 Proprietary & Confidential Sample Preparation ProductsSample Handling Products Liquid Chromatography Columns Gas Chromatography Products Chromatography Consumables Product Overview Smart Digest kits Bio-LC columns Vials, Inserts & closures Accucore core-shell columns Small molecule columns Low-flow columns GC columns GC accessories GC reagents Gas filters Micro plates & mats Virtuoso Vial Identification System Sola SPE cartridges SPE cartridges Sample filtration
- 12. 13 Proprietary & Confidential Automated, photometric testing for water, wine, beer, juice, food and enzymes Discrete Industrial Analyzer Common Water Quality Tests Phosphate Chloride Nitrate Nitrite Ammonia Hardness and Alkalinity Fluoride Ready for immediate analysis; no reagent priming, no method changeover time Gallery Discrete Analyzers have very fast sample throughput from 200 – 350 tests/hour Achieves low detection levels (to ppb levels) using proven photometric technology
- 13. 14 Proprietary & Confidential Vanquish UHPLC
- 14. 15 620 bar Thermo ScientificTM UltiMateTM 3000 Basic Automated •Highly economic & reliable Up to 1000 bar 1500 bar Vanquish Horizon •High pressure binary solvent mixing •Industry-leading precision and accuracy •Two thermostatting modes •Unmatched detection sensitivity •Biocompatible •Integration of multiple detection technologies Vanquish Flex •High pressure binary and low pressure quaternary solvent mixing options •Two thermostatting modes •Biocompatible •Integration of multiple detection technologies Thermo ScientificTM UltiMateTM 3000 SD •Workhorse for standard HPLC applications Thermo ScientificTM UltiMateTM 3000 RS/BioRS •Specialty workflow support •Binary and quaternary UHPLC systems 800-1200 bar Thermo ScientificTM UltiMateTM 3000 RSLCnano Thermo Scientific™ EASY-nLC™ 1200 •UHPLC systems for Nano/Cap/Micro range •Ideal front-end for proteomics applications HPLC systems UHPLC systems Routine analysis Low-flow Flexibility, more performance and method development Research & HT Thermo Scientific HPLC Portfolio
- 15. 16 Vanquish Platform Thermo Scientific™ Vanquish™ Flex Quaternary system • 1000 bar Quaternary System • Medium Gradient Delay Volume UHPLC for resolution or method development Highest performance for best separation or throughput Thermo Scientific™ Vanquish™ Horizon system • 1500 bar Binary System • Ultra low Gradient Delay Volume Vanquish Systems – Comparison Thermo Scientific™ Vanquish™ Flex Binary system • 1000 bar Binary System • Low Gradient Delay Volume Throughput for targeted UHPLC separations • Improved retention time precision • Biocompatible by default • Increased sample capacity • Active and passive pre-heating • Many detection options • Platform-inherent robustness & maintenance features
- 16. 17 Column efficiency improvement - Van Deemter plots 5m 1.9m u (mm/s) H(m) 5 10 15 0 61 2 3 4 5 3m IncreasingColumnEfficiency(N) pd N 1 L N H Increasing Flow Rate Faster runs with better efficiency and without compromise in performance!
- 17. 18 Vanquish Horizon Delivers Method Speed-Up Standard Method Sequence Coverage 100% 100% 100% 100% 100% Acclaim 120 C18 column, 2.1 x 250 mm, 2 - 45% ACN + 0.1% FA, 60℃. 5 10 15 20 25 [min] Time (min) 14.64 20.23 10.592.73 10.78 14.52 7.99 2.70 6.97 9.43 5.20 1.86 4.45 6.02 3.35 1.13 3.12 4.12 2.43 1.08 30 min gradient 0.4 mL min-1 600 bar 20 min gradient 0.4 mL min-1 600 bar 13 min gradient 0.6 mL min-1 850 bar 8 min gradient 1.0 mL min-1 1150 bar 5 min gradient 1.1 mL min-1 1300 bar • Vanquish used for high throughput peptide mapping • Gradient time reduced from 3 to 5 min with constant 100% sequence coverage • Thermo Scientific™ Q Exactive™ HF hybrid quadrupole-Orbitrap mass spectrometer delivers enough data points per chromatographic peak • Vanquish is able to speed up peptide mapping significantly
- 18. 19 Proprietary & Confidential Vanquish – A Completely New Developed Autosampler • Metering device replaces syringe • Fine aspiration drive for ultra precise dosage • Sample pre-compression for outstanding retention time precision and column lifetime • Newly developed ceramic valve • Advanced air-to-air cooling • Extended sample capacity • 4 sample racks and/or well plates • Optimized fluidic design
- 19. 20 Proprietary & Confidential Experimental Results – Pre-Compression Column: Hypersil Gold, 1.9 µm, 2.1 × 100 mm System: Vanquish System Mobile Phase: A–water B–acetonitrile Gradient: Flow rate: 260 L/min Pressure: 300 bar (max.) Comp. Vol: 0 µL and 3 µL Temperature: 25 ºC Injection: 1 µL Detection: pump pressure sensor Time [min] % A %B 0 60 40 0.4 60 40 4.0 0 100 4.8 0 100 4.9 60 40 10.0 60 40 w/o pre- compression w/ pre- compression Inject command at 0.1 min to track the pressure drop isocratic step from 0 to 0.4 min (cycle completed) No pressure drop during injection Why is this important? • Reduced physical stress on column longer lifetime w/o performance loss less work • More stable flow in early gradient forming better retention time reproducibility better results more confidence
- 20. 21 Column Lifetime Stability Evaluation injection #1953 #1213 #404 Vanquish UHPLC Family: Intelligent Smart Inject feature with sample pre-compression enables excellent column performance up to 1950 injections! Vanquish Horizon & Vanquish Flex UHPLC Systems MAbPac SEC-1, 4x300 mm, 0.3 mL/min On Column Injection # Retention Time (min) Monomer Relative Peak Area (%) Monomer Peak Width @ 50% Height (min) Asymmetry (EP) Theoretical Plates (EP) 25 9.554 96.96 0.237 0.92 9032 404 9.558 97.21 0.240 0.92 8797 665 9.558 96.96 0.241 0.93 8736 1213 9.567 97.27 0.243 0.89 8604 1551 9.544 96.99 0.244 0.88 8460 1953 9.524 96.30 0.255 0.89 7737 85% initial column efficiency following 1866 mAb injections (1953 on-column injections)
- 21. 22 Proprietary & Confidential VAS – Injection Valve • Innovative new valve design by Thermo Scientific • Compact valve design for optimized flow path • Bio-compatible valve • Stator and rotor made of ceramics • Long-life DLC coating • Valve is available as an entire assembly • Valve is made for Viper fittings • Needle seat is directly installed on the injection valve • Integrated needle seat connection to the valve • Reduces extra column volume Port Connected Component 1 Inlet capillary from the pump 2 Outlet capillary to the column 3 Needle seat (pre-installed) 4 Wash port waste (pre-installed) 6 Metering device (pre-installed)
- 22. 23 Proprietary & Confidential • Bioinert 1/32″ capillary • Finger tight, correct fit every time! • Universal • Virtually zero-dead volume connections • Ease of use, one-piece design PEEK Seal Removable knurl Adapter (no ferrule!) Finger Tight Screw Bioinert 1/32″ capillary Biocompatible Viper™ Fingertight Fitting System
- 23. 24 Proprietary & Confidential Vanquish – Column Compartment • High temperature accuracy and precision to host 2 columns • Temperature range 5 – 120˚C • Support of forced air and still air operating modes • Actively controlled pre-heater • Upgradeable with up to two switching valves • Expandable for multiple columns and temperature zones
- 24. 25 Three Workflows. Two Flow Paths. One Integrated UHPLC Solution. Dual LC • A two channel LC system with standard footprint Tandem LC/LC-MS • System for efficient detector utilization Inverse gradient • System for advanced quantification capabilities