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Culturing of bacteria
In solid media
Bacteria:
 Bacteria is a single-celled (unicellular)
microorganism that does not have a nucleus or
any other membrane-bound organelles.
Purpose of culturing:
 Culturing is a method of multiplying microbial
organisms by letting them reproduce in
predetermined culture media under controlled
laboratory conditions. Microbial cultures are
foundational and basic diagnostic methods used
extensively s a research tool in molecular
biology.
Objectives:
 To become familiar with the necessary nutrients
and environmental factors for culturing of
microorganisms in laboratory
 To understand the decontamination or
sterilization process using an autoclave
 To learn the procedure used in preparing media
needs for culturing of microorganisms
Continued..
 Isolation of bacteria
 Maintenance of pure stock culture and standard
culture
 Enumeration of bacteria in samples
 Increasing the number of bacteria so as we get
them in visible form as colonies
Principles:
 Bacteria are cultured in sterilized, nutritionally
rich liquid or solid media
 The liquid media Broth is taken in test tube and
Solid media Agar is contained in Petri dish to form
Agar plate
 Cultivation of bacteria needs some material which
is suspected to contain bacteria
 On solid Agar plate Bacteria grows as colonies.
Each colony growing form a single bacteria
Required Materials:
 Equipment:
Chemicals:
 Ethyl alcohol
 Sodium chloride
 0.1M HCL
 0.1M NaOH
 Distilled water
 LB material
 Agar
 Tryptone and Yeast extract
Cultivation:
Cultivation is done by following steps:
Preparation of lab materials
Sterilization
Inoculation
incubation
Observation
Preparation of lab materials:
Agar mixed
in tube
Placed in
autoclave
Poured in
petri dishes
Cool at room
temperature
Sterilization:
 Glass wares are sterilized in hot air at 180°C for 3
hours and media in autoclave at 121°C for 15
minutes.
Inoculation:
Mortar and pestle Pipetting sample
Incubation:
 The inoculated plates are incubated at 37°C for
24 hours in an incubator.

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Slides of solid media

  • 2. Bacteria:  Bacteria is a single-celled (unicellular) microorganism that does not have a nucleus or any other membrane-bound organelles.
  • 3. Purpose of culturing:  Culturing is a method of multiplying microbial organisms by letting them reproduce in predetermined culture media under controlled laboratory conditions. Microbial cultures are foundational and basic diagnostic methods used extensively s a research tool in molecular biology.
  • 4. Objectives:  To become familiar with the necessary nutrients and environmental factors for culturing of microorganisms in laboratory  To understand the decontamination or sterilization process using an autoclave  To learn the procedure used in preparing media needs for culturing of microorganisms
  • 5. Continued..  Isolation of bacteria  Maintenance of pure stock culture and standard culture  Enumeration of bacteria in samples  Increasing the number of bacteria so as we get them in visible form as colonies
  • 6. Principles:  Bacteria are cultured in sterilized, nutritionally rich liquid or solid media  The liquid media Broth is taken in test tube and Solid media Agar is contained in Petri dish to form Agar plate  Cultivation of bacteria needs some material which is suspected to contain bacteria  On solid Agar plate Bacteria grows as colonies. Each colony growing form a single bacteria
  • 8. Chemicals:  Ethyl alcohol  Sodium chloride  0.1M HCL  0.1M NaOH  Distilled water  LB material  Agar  Tryptone and Yeast extract
  • 9. Cultivation: Cultivation is done by following steps: Preparation of lab materials Sterilization Inoculation incubation Observation
  • 10. Preparation of lab materials: Agar mixed in tube Placed in autoclave Poured in petri dishes Cool at room temperature
  • 11. Sterilization:  Glass wares are sterilized in hot air at 180°C for 3 hours and media in autoclave at 121°C for 15 minutes.
  • 12. Inoculation: Mortar and pestle Pipetting sample
  • 13. Incubation:  The inoculated plates are incubated at 37°C for 24 hours in an incubator.