This document discusses various methods for measuring biotic factors and biodiversity in ecosystems. It describes how species richness is simply the number of different species counted, while biodiversity combines both the number of species and their relative abundances. It provides Simpson's Biodiversity Index formula for calculating biodiversity. Population size can be estimated by throwing quadrats and scaling up based on total area. The Lincoln Index uses mark-recapture data to estimate animal population sizes. Other methods like measuring abundance, density, frequency, cover, and biomass are also discussed. The document considers potential issues with methods and ethical considerations.