Tools of biotechnology
- 2. Recombinant dna technologyRecombinant dna technology A technology which involves theA technology which involves the combining or rejoining of dnacombining or rejoining of dna fragment from two differentfragment from two different organismsorganisms
- 3. Basics steps in rDNA technologyBasics steps in rDNA technology 1.1.isolation of dna fragment containing aisolation of dna fragment containing a gene of interest.gene of interest. 2.Generation of a recombinant dna2.Generation of a recombinant dna molecule by insertion of dnamolecule by insertion of dna fragment into a carrier dna molecule.fragment into a carrier dna molecule. 3.3.transfer of the rDNA into an e.coli hosttransfer of the rDNA into an e.coli host cellcell 4.selection of only those host cells4.selection of only those host cells carrying the rDNA and allowing them tocarrying the rDNA and allowing them to multiplymultiply
- 4. Tools of Recombinant DNA techTools of Recombinant DNA tech 1.restriction enzymes1.restriction enzymes 2.vectors2.vectors 3.host cell3.host cell
- 5. RESTRICTION ENZYMERESTRICTION ENZYME The molecular scissorThe molecular scissor
- 6. These are the protein molecules also called as theThese are the protein molecules also called as the molecular scissor becouse they helpto cut the dna atmolecular scissor becouse they helpto cut the dna at specific sitesspecific sites properties:-properties:- 1.A restriction enzyme that1.A restriction enzyme that selectively recognise a specific DNAselectively recognise a specific DNA sequence and digest any DNAsequence and digest any DNA fragment containing that sequence.fragment containing that sequence. 2.A modification enzyme that2.A modification enzyme that adds a methyle grup to one or twoadds a methyle grup to one or two bases with in the sequencebases with in the sequence recognised by the enzyme.recognised by the enzyme.
- 7. Some restriction enzymesSome restriction enzymes
- 8. vectorsvectors Vehicles for cloningVehicles for cloning
- 9. plasmidsplasmids Plasmids are extracromosomal,self-Plasmids are extracromosomal,self- replicate,usually circular,doublereplicate,usually circular,double stranded DNA molecule foundstranded DNA molecule found naturally in many bacteria and innaturally in many bacteria and in some yeasts.some yeasts.
- 10. Some plasmidsSome plasmids 1.PBR322 2.puc 191.PBR322 2.puc 19
- 13. Thank for giving me thisThank for giving me this opportunityopportunity Have a nice dayHave a nice day