Types of rotors in centrifugation main three types
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Centrifuge rotors are classified into three main types - fixed angle , swinging-bucket, and vertical rotors, each with specific advantages for different applications.
Types of rotors in centrifugation main three types
- 1. •• Types Of Rotors ••
- 2. What is centrifugation.. A centrifuge is a mechanical device used for separating substances of different densities using the principle of centrifugal force. A common centrifuge is a container that is revolved at high speeds. The principle of separation is similar to that of sedimentation by gravity. But in a centrifuge the driving force is much higher because the force results from the rotation of the liquid in the container. In the case of sedimentation, the driving force comes from the difference in density between the solid particles and the medium in which the particles are suspended. In a centrifuge, on the other hand, separation is effected by a force that is 1000 to 100,000 times that of gravity. The principal components of a centrifuge are a rotor and motor. The rotor is connected to a central shaft and has, at the distal end, provision for holding the container. The shaft is connected to a motor, which can be operated at different speeds.
- 3. Types of centrifuge Rotors:- Swinging bucket rotor- Fixed angle rotor- Vertical tube rotor- Zonal rotor-
- 4. Swinging bucket Rotors:- *Description: Buckets swing out horizontally during centrifugation * Advantages: Ideal for separating substances with large density differences * Disadvantages: Longer run times
- 5. Fixed Angle Rotors:- * Description: Tubes held at a fixed angle * Advantages: Shorter run times, good for separating substances with small density differences * Disadvantages: Pellets may not be evenly distributed
- 6. Vertical Rotors:- * Description: Tubes held vertically * Advantages: Very short run times, ideal for density gradient centrifugation * Disadvantages: Specialized use
- 7. Zonal Rotor:- Advantages:- * Can separate a wide range of particles * High-resolution separations * Can process large volumes of sample Disadvantages:- * Complex to operate * Require specialized equipment and expertise
- 8. Importance of choosing the right rotor:- * Optimal Separation: The correct rotor ensures the best possible separation of your target substances, maximizing yield and purity. * Reduced Run Times: Matching the rotor to the separation needs minimizes centrifugation time, improving lab efficiency. * Sample Integrity: Using the appropriate rotor prevents damage to delicate samples due to excessive forces or improper pelleting. * Experimental Success: Selecting the right rotor is crucial for achieving accurate and reproducible results, leading to successful experiments. * Safety: Incorrect rotor selection can lead to rotor failure and potential safety hazards.
- 9. References:- Text book of botany practical by Bendre kumar. Image by:- https://www.google.com/gasearch?q=rotor&source=sh/x/gs/m2/5
- 10. Thank You