Next generation sequencing
Download as PPTX, PDF4 likes734 views
Next generation sequencing (NGS) relies on template preparation using methods like emulsion PCR or solid-phase amplification followed by sequencing and imaging techniques such as sequencing by synthesis (Illumina) or pyrosequencing (454). The sequenced data is then aligned and assembled into a genome. NGS provides faster and more affordable DNA sequencing compared to previous Sanger methods.
Next generation sequencing
- 1. الرحیم الرحمن هللا بسم
- 2. Next Generation Sequencing By: Ehsan Sepahi
- 3. A Timeline of genetics
- 6. Next Generation Sequencing • The automated Sanger method is considered as a ‘first-generation’ technology, and newer methods are referred to as next-generation sequencing (NGS) • These newer technologies constitute various strategies that rely on a combination of: • template preparation • sequencing and imaging • and genome alignment and assembly
- 7. Next Generation Sequencing Platforms
- 8. Template preparation • Clonally amplified templates • emulsion PCR (emPCR) • solid-phase amplification • Single-molecule templates • primer immobilized • template immobilized • polymerase immobilized
- 14. sequencing and imaging • Cyclic reversible termination (Sequencing by synthesis): Illumina, Helicos BioSciences • Sequencing by ligation: SOLiD • Pyrosequencing: 454 • Real-time sequencing: Pasific BioSciences • Ion Semiconductor: Ion Torrent
- 17. Pyrosequencing
- 21. A Timeline of genetics
- 22. First they ignore you, then they laugh at you, then they fight you, then you win. Mahatma Gandhi